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Restriction Enzyme Analysis of Lambda DNA DNA Fingerprinting of lambda DNA Purpose • 1. See the DNA • 2. Become familiar with -Restriction enzymes -Electrophoresis -Analysis of DNA bands Applications of the procedure • Used to identify bacteria and viruses based on the DNA finger printing of these organisms. • Genetic screening – electrophoresis is the first step. • Forensic medicine – electrophoresis is used in DNA finger printing. • Lambda Phage infects E.coli. • Its DNA is about 49,000 base pairs long • Restrictions enzymes are molecular scissors • Recognize specific sequence cut DNA make cuts in the DNA • Each enzyme recognizes different sequence. • • • • Gel is porous DNA fragments migrate through the gel Electric current push the DNA DNA is negatively charged because of the phosphates • Migrate from negative to positive • Smaller fragments migrate faster • Each band has many copies of a fragment. DNA Fingerprinting 1 The dark blue lines are DNA fragments trapped in the gel. DNA fingerprinting is some times used to identify microbes.