Download Chapter 10 #1

Molecular Biology of the Gene
DNA Lesson Part 1
Dr. Wilson Muse
Schoolcraft College
10.1 Experiments showed that DNA
is the genetic material
– Frederick Griffith discovered that a
“transforming factor” could be transferred into a
bacterial cell
– Disease-causing bacteria were killed by heat
– Harmless bacteria were incubated with heat-killed
bacteria
– Some harmless cells were converted to diseasecausing bacteria, a process called transformation
– The disease-causing characteristic was inherited
by descendants of the transformed cells
Copyright © 2009 Pearson Education, Inc.
10.1 Experiments showed that DNA
is the genetic material
– Alfred Hershey and Martha Chase used
bacteriophages to show that DNA is the genetic
material
– Bacteriophages are viruses that infect bacterial cells
– Phages were labeled with radioactive sulfur to detect
proteins or radioactive phosphorus to detect DNA
– Bacteria were infected with either type of labeled
phage to determine which substance was injected into
cells and which remained outside
Copyright © 2009 Pearson Education, Inc.
10.1 Experiments showed that DNA
is the genetic material
– The sulfur-labeled protein stayed with the phages
outside the bacterial cell, while the phosphoruslabeled DNA was detected inside cells
– Cells with phosphorus-labeled DNA produced new
bacteriophages with radioactivity in DNA but not in
protein
Animation: Hershey-Chase Experiment
Animation: Phage T2 Reproductive Cycle
Copyright © 2009 Pearson Education, Inc.
Head
DNA
Tail
Tail fiber
Head
DNA
Tail
Tail fiber
Radioactive
protein
Phage
Bacterium
Empty
protein shell
Radioactivity
in liquid
Phage
DNA
DNA
Batch 1
Radioactive
protein
Centrifuge
Pellet
2 Agitate in a blender to
1 Mix radioactively
labeled phages with
bacteria. The phages
infect the bacterial cells.
Batch 2
Radioactive
DNA
separate phages
outside the bacteria
from the cells and
their contents.
3 Centrifuge the mixture
so bacteria form a
pellet at the bottom of
the test tube.
4 Measure the
radioactivity in
the pellet and
the liquid.
Radioactive
DNA
Centrifuge
Pellet
Radioactivity
in pellet
Phage
Radioactive
protein
Bacterium
Phage
DNA
DNA
Batch 1
Radioactive
protein
2 Agitate in a blender to
1 Mix radioactively
labeled phages with
bacteria. The phages
infect the bacterial cells.
Batch 2
Radioactive
DNA
Empty
protein shell
separate phages
outside the bacteria
from the cells and
their contents.
Radioactive
DNA
Empty
protein shell
Radioactivity
in liquid
Phage
DNA
Centrifuge
Pellet
3 Centrifuge the mixture
so bacteria form a
pellet at the bottom of
the test tube.
4 Measure the
radioactivity in
the pellet and
the liquid.
Centrifuge
Pellet
Radioactivity
in pellet
Phage attaches
to bacterial cell.
Phage injects DNA.
Phage DNA directs host
cell to make more phage
DNA and protein parts.
New phages assemble.
Cell lyses and
releases new phages.
DNA
• DNA is often called
the blueprint of life.
• In simple terms,
DNA contains the
instructions for
making proteins
within the cell.
Nucleotides
O
O -P O
O
O
O -P O
O
One deoxyribose together with its
phosphate and base make a
nucleotide.
O
O -P O
O
Phosphate
Nitrogenous
base
O
C
C
C
O Deoxyribose
10.2 DNA and RNA are polymers
of nucleotides
– The monomer unit of DNA and RNA is the
nucleotide, containing
– Nitrogenous base
– 5-carbon sugar
– Phosphate group
Copyright © 2009 Pearson Education, Inc.
– DNA and RNA are polymers called
polynucleotides
– A sugar-phosphate backbone is formed by
covalent bonding between the phosphate of one
nucleotide and the sugar of the next nucleotide
– Nitrogenous bases extend from the sugarphosphate backbone
Animation: DNA and RNA Structure
Copyright © 2009 Pearson Education, Inc.
Sugar-phosphate backbone
Phosphate group
Nitrogenous base
Sugar
Nitrogenous base
(A, G, C, or T)
DNA nucleotide
Phosphate
group
Thymine (T)
Sugar
(deoxyribose)
DNA nucleotide
DNA polynucleotide
Nitrogenous base
(A, G, C, or T)
Phosphate
group
Thymine (T)
Sugar
(deoxyribose)
Thymine (T)
Cytosine (C)
Pyrimidines
Adenine (A)
Guanine (G)
Purines
Nitrogenous base
(A, G, C, or U)
Phosphate
group
Uracil (U)
Sugar
(ribose)
Hydrogen Bonds, cont.
• When making
hydrogen bonds,
cytosine always pairs
up with guanine,
• And adenine always
pairs up with
thymine.
• (Adenine and thymine
are shown here.)
O
N
O
C
C
C C
N
C
C
CC
N
N
N
C
N
O
• The bases attract each
other because of
hydrogen bonds.
• Hydrogen bonds are
weak but there are
millions and millions
of them in a single
molecule of DNA.
• (The bonds between
cytosine and guanine
are shown here.)
C
N
Hydrogen Bonds
Twist
Hydrogen bond
Base
pair
Ribbon model
Partial chemical structure
Computer model
Base
pair
Ribbon model
Hydrogen bond
Partial chemical structure
10.4 DNA replication depends on
specific base pairing
– DNA replication follows a semiconservative
model
– The two DNA strands separate
– Each strand is used as a pattern to produce a
complementary strand, using specific base pairing
– Each new DNA helix has one old strand with one
new strand
Animation: DNA Replication Overview
Copyright © 2009 Pearson Education, Inc.
Two Stranded DNA
• Remember, DNA
has two strands
that fit together
something like a
zipper.
• The teeth are the
nitrogenous bases
but why do they
stick together?
DNA by the numbers
• Each cell has about 2 m
of DNA.
• The average human has
75 trillion cells.
• The average human has
enough DNA to go from
the earth to the sun
more than 400 times.
• DNA has a diameter of
only 0.000000002 m.
The earth is 150 billion m
or 93 million miles from
the sun.
DNA REPLICATION
Makin’ copies ........
Copyright © 2009 Pearson Education, Inc.
10.4 DNA replication depends on
specific base pairing
– DNA replication follows a semiconservative
model
– The two DNA strands separate
– Each strand is used as a pattern to produce a
complementary strand, using specific base pairing
– Each new DNA helix has one old strand with one
new strand
Animation: DNA Replication Overview
Copyright © 2009 Pearson Education, Inc.
Parental
molecule
of DNA
Nucleotides
Parental
molecule
of DNA
Both parental
strands serve
as templates
Nucleotides
Parental
molecule
of DNA
Both parental
strands serve
as templates
Two identical
daughter
molecules of DNA
10.5 DNA replication proceeds in two directions at many
sites simultaneously
– DNA replication begins at the origins of
replication
– DNA unwinds at the origin to produce a “bubble”
– Replication proceeds in both directions from the
origin
– Replication ends when products from the bubbles
merge with each other
– DNA replication occurs in the 5’ 3’ direction
– Replication is continuous on the 3’ 5’ template
– Replication is discontinuous on the 5’
template, forming short segments
Copyright © 2009 Pearson Education, Inc.
3’
10.5 DNA replication proceeds in
two directions at many sites
simultaneously
– Proteins involved in DNA replication
– DNA polymerase adds nucleotides to a growing
chain
– DNA ligase joins small fragments into a
continuous chain
Animation: Origins of Replication
Animation: Leading Strand
Animation: Lagging Strand
Animation: DNA Replication Review
Copyright © 2009 Pearson Education, Inc.
Origin of replication
Parental strand
Daughter strand
Bubble
Two daughter DNA molecules
5 end
P
5
4
3
2
1
P
3 end
2
3
1
4
5
P
P
P
P
P
P
3 end
5 end
DNA polymerase
molecule
5
3
3
5
Daughter strand
synthesized
continuously
Parental DNA
3
5
5
3
DNA ligase
Overall direction of replication
Daughter
strand
synthesized
in pieces
THE FLOW OF GENETIC
INFORMATION FROM DNA
TO RNA TO PROTEIN
Copyright © 2009 Pearson Education, Inc.
10.6 The DNA genotype is
expressed as proteins, which
provide the molecular basis for
phenotypic traits
– A gene is a sequence of DNA that directs the
synthesis of a specific protein
– DNA is transcribed into RNA
– RNA is translated into protein
– The presence and action of proteins determine
the phenotype of an organism
Copyright © 2009 Pearson Education, Inc.
10.6 The DNA genotype is
expressed as proteins, which
provide the molecular basis for
– Demonstratingphenotypic
the connectionstraits
between genes
and proteins
– The one gene–one enzyme hypothesis was based
on studies of inherited metabolic diseases
– The one gene–one protein hypothesis expands the
relationship to proteins other than enzymes
– The one gene–one polypeptide hypothesis
recognizes that some proteins are composed of
multiple polypeptides
Copyright © 2009 Pearson Education, Inc.
DNA
Nucleus
Cytoplasm
DNA
Transcription
RNA
Nucleus
Cytoplasm
DNA
Transcription
RNA
Nucleus
Cytoplasm
Translation
Protein