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The Diversity of Life Classification, Viruses, Prokaryotes, Protists and Fungi Magnet: Parts of Chapters 20-23 Honors: Parts of Chapters 17 -20 Classification of organisms • Taxonomy-Discipline of Bio that deals with identifying, naming, classifying, organisms – Aristotle- Grouped organisms as plants or animals Grouped animals based on habitat; plants based on structure (morphology). Believed species were fixed • Linnaeus-Father of taxonomy. Classified species based on natural relationships ( behavior, structure and habitat) • Systematics is a broader science that deals with taxonomy and evolutionary history • Binomial nomenclature-2 word Latin name • Taxons: Domain, Kingdom, Phylum, Class, Order, Family, Genus, species • Species-group of organisms that are able to produce viable offspring • Phyla in plants are called divisions • Dichotomous keys How are relationships determined? • Evolutionary history (phylogeny). Cladisticsclassifies organisms according to the order they diverged from a common ancestor. See cladograms (phylogenic trees)- p. 354. Sequences orders of organisms based on derived characters that evolved with respect to a common outgroup • Development and Behavior • Biochem (Nucleic acids and amino acids) and Genetics • HW- What is a molecular clock? Traditional (“old”)5 Kingdom System • “Older” classification system (before domains): Kingdoms Monera, Protista, Fungi. Plantae, Animalia • But now we have added domains….Monerans are now divided into 2 domains-Archeae and Bacteria. 3rd domain is Eukarya. According to the cladogram on p. 354 • , which 2 domains are more closely related? • Some classification systems are now dividing K. Protista into 3 kingdoms Evidence for the 3 domain system • Distinct differences in the rRNA sequence between 2 groups of prokaryotes • DNA sequencing data • Membrane structure • Cell wall structure: bacteria cell wall is made of peptidoglycan. Archaea have proteins in their walls similar to the ones found in our membranes • STUDY TABLE 20.3!!!!!!!! Viruses • Made of protein coat (capsid) and nucleic acid • 5-300 nm (nm is a billionth of a meter) • Why aren’t they considered “living”? • No “cure”. Some can be prevented by vaccination • Ex- influenza, cold, measles, mumps, HIV, hepatitis, chicken pox, herpes Reproduction • Intracellular parasites • Virus attaches to host cell using their coat’s proteins and the host’s cell membrane receptors. Viral genome then enters host cell • Viruses can be made of DNA or RNA---HIV is a retrovirus made of RNA • Lytic cycle(active-lysis) vs. Lysogenic (inactivevirus hidden as prophage). HW-What is a prion? Give an example Bacteria SHAPES • Cocci-spheres • Spirillum (spirochete)-spiral (helical) • Bacillum-rod-shaped Typical bacterial cell • • • • Know structure p. 63 Unicellular Prokaryotic Large circular chromosome; plasmids in nucleiod region • Cell walls (peptidoglycan); Many secrete sticky substance that forms capsule outside wall. Both surround DNA. • Usually 1-10 um long • Cell wall prevents osmotic rupture. Penicillin breaks down cell wall and allows rupture Bacteria continued • Some use O2—others are anaerobes (may be obligate or facultative) • Some are flagellated • Fimbriae (once called nonsexual pili)- help bacteria to adhere to surfaces. • Pili (sexual)--used for conjugation • Reproduce asexually by binary fission • Endospore- resistant structure with a thick, protective coat protecting a bacterium inside. Can survive for years before rehydrating. P. 369 • Some bacteria have an additional outer “coat” containing lipid. Those that have it are not able to absorb a “dye” called a gram stain and are called gram -. Those without it (gram +) can absorb it and appear purple. Technique is often used in (medical) labs to differentiate types and narrow down possible diseases . Prokaryotes are the foundation of life on earth • Decompose dead organisms – Decomposers, saprobes, saprophytes • Perform nitrogen fixation • Live in our digestive system and are also used in the food industry – Cheese, yogurt, etc • Used to decompose waste in sewage • Disesase causing bacteria – Usually produce toxins. Ex- bacteria that causes botulism (paralyzes nerve cells) Some Prokaryotes Cause Disease • Bacterial Examples: cholera, diptheria, leprosy, Lyme disease, meningitis, the plague, pneumonia, sphylisis, tetanus, tuberculosis, strep throat. See p. 375 • Antibiotics are the most effective means of fighting bacterial infections • **No known Archaea cause disease Major Groups of Archaea • Extremophiles-3 types • Methanogens- are poisoned by oxygen;use CO2 as the electron acceptor in respiration; produces methane as a waste product • Halophiles- lives in very saline places • Thermophiles (aka hyperthermophiles) Archaea • Used to be grouped with bacteria and called monerans • Now believed eukaryotes “split” from archaeal line of descent • Archaea and Eukarya share some of the same ribosomal proteins and similar tRNA • Archaea have “unusual” lipids in membrane that allow them to live under extreme conditions • Cell walls composed of polysacc and some only are entirely protein. **A few recently discovered have no wall (not on test, just FYI) The Origin of the Eukaryotic Cell • Eukaryotic cells arose through a combination of 2 processes: -membrane infolding- produced all the membrane-bound organelles except the mitochondrion and the choloroplasts. – Endosymbiosis-Mitochondria and chloroplasts believed to once be prokaryotic cells that were ingested or absorbed by eukaryotic cell. Kingdom Protista • • • • • Domain Eukarya Very diverse group of organisms Most are unicellular The most elaborate cells of all the kingdoms Most are aerobic & use mitochondria for respiration • Some are autotrophs, some are heterotrophs • 3 types: ingestive, absorptive, photosynthetic. Kingdom Protista • Motility: flagella, cilia, pseudopodia • Some reproduce sexually, some reproduce asexually - the haploid stage is the main vegetative stage of most protists; only the zygote is diploid. Zygotes undergo meiosis and become haploid (see life cycle in book) • Can form cysts that survive harsh conditions • Most are aquatic (plankton). What adaptation prevents them from lysing in water? Protozoa – animal-like protists • Heterotrophic and ingestive; Grouped by their means of locomotion (only know these): 1-ciliophorans (P. Ciliophora) – move with _______; examples include Paramecium & Stentor & Blepharisma **paramecium have a macronucleus (for everyday metabolism) and micronucleus (reproduction) 2 -zooflagellates (P. Zoomastigophora) – move with ________; examples include Giardia, Trypanasoma 3 -sarcondines (**some texts: P. Sarcodina; others: P. Rhizopoda) – move with _______; examples include amoeba 4 -sporozoans (P. Apicomplexa/ P. Sporozoa) – don't move; parasitic; Plasmodium Algae – the plant-like protists • Autotrophic • Classified by the pigments they contain • All of the algae contain chlorophyll (photosynthetic), but some contain different types of chlorophyll and accessory pigments, causing them to appear other colors than green. The Plant-Like Protists • P. Chlorophyta – green algae Chlamydomonas, Volvox, some seaweeds, sea lettuce • P. Chrysophyta – golden-brown algae -Ex: Diatoms! Contain silica (**some books are classifying this differently now, but I am still going with this) • P. Euglenophyta – Ex: Euglena (are photosynthetic, but can ingest if too deep in water to get light) • P. Dinoflagellata/ P. Pyrrophyta -dinoflagellates – cause red tide (toxic to fish) More Plant-Like Protists • P. Phaeophyta – the brown algae -include the largest seaweeds, the kelps (**multicellular) • P. Rhodophyta – the red algae -include the red seaweeds, some encrusted and common in coral reefs The Fungus Like Protists • Unicellular, heterotrophic, absorbative (usually feed on decaying matter) • Cell walls mainly made of cellulose (like plants). No chitin, which is found in true fungal walls • Most known as slime molds or water molds • When food is not plentiful, they produce spore producing structures (sporangia) and the wind disperses the spores • Ex- slime molds Lichens • Look similar to some species of moss, but are not. • Lichens are symbiotic associations between a fungus (often an ascomycete) and green algae or cyanobacteria • The fungus usually give lichens “shelter” (optimal environment) which gives rise to their shape • Alga provides the fungus with food • Fungus provides a suitable physical environment for growth What is a fungus? • • • • Heterotrophic Most are muticellular Nonphotosynthetic Digest food outside bodies (using enzymes) and absorb it • some are saprophyteslive off of dead organic matter) • Cell walls made of chitin. Structure and Function of Multicellular Fungi • Composed of tiny filaments called hyphae • Hyphae are long strings of cells. Mass called mycelium. Some species can grow a km of hyphae/day! • Can reproduce asexually by releasing haploid spores • Unicellular fungi-yeast • Multicellular ex: mushrooms, molds Life cycle • See supplement for life cycle of mushroom • Stages of a mushroom (Basidiomycetes): – Dikaryotic (contains 2 haploid nuclei/cell) – Diploid- Haploid nuclei fuse in fruiting body of mushroom forming diploid zygote – Haploid-Zygote undergoes meiosis and forms haploid spores. Spores then germinate and fuse. Classification • Phyla: – Zygomycota- Common mold(ex-Rhizopus). Reproduce by conjugation – Ascomycota- Sac fungi; Contain ascus which are sac like structures that contain spores. Ex-Yeast, mildew – Basidiomycota-Have fruiting bodies (ex-mushroom’s cap). Contain basidium which are spore bearing repro. structures on the gills of the mushroom caps – Deuteromycota: Imperfect fungi. Sexual repro. has not been observed. Ex: Penicillium, ringworm, athletes foot Zygomycota Ascomycota Basidiomycota Deutermycota Mycorrhizae (“fungus roots”) • Mutualistic associations of plant roots and fungi • Almost all vascular plants have mychorrhizae Ecological Impacts of Fungi • Decomposers! Important nutrient recyclers • Some are pathogens, such as ringworm and athletes foot.Plant pathogens--Dutch elm disease, Chestnut blight • some produce deadly toxins-ex-some species of mushrooms • we use them for their antibiotics-penicillium. Produce enzymes that rupture bacterial cell walls