Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Zinc finger nuclease wikipedia , lookup
DNA repair protein XRCC4 wikipedia , lookup
Microsatellite wikipedia , lookup
Homologous recombination wikipedia , lookup
United Kingdom National DNA Database wikipedia , lookup
DNA polymerase wikipedia , lookup
DNA nanotechnology wikipedia , lookup
DNA replication wikipedia , lookup
DNA Structure and Function Chapter 13 13.1 Discovery Of DNA Function Griffith Discovers Transformation • 1928 • Attempting to develop a vaccine • Isolated two strains of Streptococcus pneumoniae – Rough strain was harmless – Smooth strain was pathogenic Griffith Discovers Transformation 1. Mice injected with live cells of harmless strain R. 2. Mice injected with live cells of killer strain S. 3. Mice injected with heat-killed S cells. 4. Mice injected with live R cells plus heatkilled S cells. Mice live. No live R cells in their blood. Mice die. Live S cells in their blood. Mice live. No live S cells in their blood. Mice die. Live S cells in their blood. Figure 13.3 Page 218 Transformation • What happened in the fourth experiment? • The harmless R cells had been transformed by material from the dead S cells • Descendents of the transformed cells were also pathogenic Oswald & Avery • What is the transforming material? • Cell extracts treated with proteindigesting enzymes could still transform bacteria • Cell extracts treated with DNA-digesting enzymes lost their transforming ability • Concluded that DNA, not protein, transforms bacteria Bacteriophages • Viruses that infect bacteria • Consist of protein and DNA • Inject their hereditary material into bacteria bacterial cell wall plasma membrane cytoplasm Figure 13.4b Page 219 Hershey & Chase’s Experiments • Created labeled bacteriophages – Radioactive sulfur – Radioactive phosphorus • Allowed labeled viruses to infect bacteria • Asked: Where are the radioactive labels after infection? virus particle labeled with 35S Hershey and Chase Results virus particle labeled with 32P bacterial cell (cutaway view) label outside cell Figure 13.5 Page 219 label inside cell 13.2 DNA Structure Nucleotides in DNA • Each nucleotide consists of – Deoxyribose (5-carbon sugar) – Phosphate group – A nitrogen-containing base • Four bases – Adenine, Guanine, Thymine, Cytosine Nucleotide Bases ADENINE (A) phosphate group GUANINE (G) deoxyribose THYMINE (T) CYTOSINE (C) Figure 13.6 Page 220 Composition of DNA • Chargaff showed: – Amount of adenine relative to guanine differs among species – Amount of adenine always equals amount of thymine and amount of guanine always equals amount of cytosine A=T and G=C Watson-Crick Model • DNA consists of two nucleotide strands • Strands run in opposite directions • Strands are held together by hydrogen bonds between bases • A binds with T and C with G • Molecule is a double helix Watson-Crick Model Figure 13.7 Page 221 13.4 DNA Replication and Repair Structure Helps Explain How It Duplicates • DNA is two nucleotide strands held together by hydrogen bonds • Hydrogen bonds between two strands are easily broken • Each single strand then serves as template for new strand DNA Replication • Each parent strand remains intact • Every DNA molecule is half new old old new “old” and half “new” Figure 13.9 Page 222 Base Pairing during Replication Each old strand serves as the template for complementary new strand Figure 13.10 Page 223 Enzymes in Replication • Enzymes unwind the two strands • DNA polymerase attaches complementary nucleotides • DNA ligase fills in gaps • Enzymes wind two strands together A Closer Look at Strand Assembly Energy for strand assembly is provided by removal of two phosphate groups from free nucleotides newly forming DNA strand one parent DNA strand Figure 13.10 Page 223 Continuous and Discontinuous Assembly Strands can only be assembled in the 5’ to 3’ direction Figure 13.10 Page 223 DNA Repair • Mistakes can occur during replication • DNA polymerase can read correct sequence from complementary strand and, together with DNA ligase, can repair mistakes in incorrect strand