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a b Supplemental Figure 1: Treemaps of GO enrichment results at DAG25. (a): Treemap of biological processes at DAG25 using GO enrichment results. At least 2-fold change genes at DAG25 used for GO enrichment analysis at http://omicslab.genetics.ac.cn/GOEAST/index.php, and the GO enrichment results together with their p-values (<0.05) utilized for the treemap analysis at http://revigo.irb.hr. The size of each area calculated according to the formula abs(log10(p-value)). (b): Treemap of molecular functions at DAG25 using GO enrichment results. At least 2-fold change genes at DAG25 used for GO enrichment analysis at http://omicslab.genetics.ac.cn/GOEAST/index.php, and the GO enrichment results together with their p-values (<0.05) utilized for the treemap analysis at http://revigo.irb.hr. The size of each area calculated according to the formula abs(log10(p-value)). 300 Genes in sublocation 250 200 150 DAG60 100 DAG25 50 0 Supplemental Figure 2: Distributions of at least 2-fold change regulated genes in different locations of cell at DAG60 (blue bar) and DAG25 (red bar). Supplemental Figure 3: Heatmap presentations of the genes with at least 4-fold change at DAG60 in the four samples (60W, 60M, 25W and 25M). Gene expression abundance indicated by different colors, with yellow represented strong signal and blue represented weak signal. Genes used for this presentation and Fig. 4 were listed in the Supplemental Table 2. a b Supplemental Figure 4: Prediction of protein interactions in the upregulated genes (at least 2-fold change) (a) and down-regulated genes (at least 2-fold change) (b) of htd3 at DAG60. The results predicted based on the BAR Rice Interactions Viewer (http://bar.utoronto.ca/interactions/cgibin/rice_interactions_viewer.cgi).