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Transcript
Genetic Transformation of Plants: Methods and Approaches to Develop Transgenic plants Steps in producing Transgenic -A source of foreign DNA containing the desired gene -A vector that carries the gene -Means to introduce the vector into host plant Methods to create Transgenics - Agrobacterium mediated transformation - Electroporation - Biolistic methods for direct entry - Protoplast fusion Agrobacterium mediated Transgenic Steps in T-DNA integration Plant wound --> phenolics --> sense by VirA--> signal passed to VirG --> VirG activates expression of other vir genes --> the products of vir genes help to excise TDNA from the Ti-plamsid
