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Dazl shRNA construct design
Dann C T et al. PNAS 2006;103:11246-11251
©2006 by National Academy of Sciences
Transgene expression and DAZL knockdown
Dann C T et al. PNAS 2006;103:11246-11251
©2006 by National Academy of Sciences
Analysis of Dazl-shRNA phenotype
Dann C T et al. PNAS 2006;103:11246-11251
©2006 by National Academy of Sciences
Reverse genetics in the rat: Advantages to using
in vivo RNAi over traditional knock-out methods
• Because of the small size of the sequences encoding shRNAs, these
sequences can be cloned in lentiviral vectors
• Production of transgenic founders with lentivirus is easy and efficient
• In vivo RNAi allows to generate animals with different degrees of gene
deficiency. An allelic series of hypomorphs is often more informative for
modeling human disease phenotypes
• Conditional in vivo RNAi approach is now feasable in the rat using tissue
specific promoters (RNA polymerase II promoters) driving the expression
of shRNAs (embedded in microRNAs)