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Figure
more
then
3 shows
rapidly,
becomes
lineor
produce
100 grams
o growth
curve
as the conidia
and
remains
of
so until
hyphoe,
Brown,
I.R.
and
0.
J.
design
II to
wet
Health
Service
Traineerunder
Washington
University,
St.
Convenient
for a typical
germinate.
12 hours,
weight,
grant
Louis,
Gillie.
at
12 hours
from
and
the
a growth
to be more
diameter
plugs.
of the
These
London,
tube,
shown
convenient
dental
W. I.
ends
rolls
and
in the
for the
the
chimney
(Numbers
Although
4 and
pyrex
tubing
I) ore
III
From
talks
to other
that
the
Ryan,
the
supplied
by
normally
Cottrell
supplied
we
of growth
of the
cotton
and
ends
wool
Co.,
to sufficiently
would
Public
Biology,
under-
tube
designed
Tatum
is still
widely
which
we believe
after
The form
of dental
use
S.
workers
form
rate
culture
(U.
Molecular
of
and
growth
This
Neurosporo
and
slowly,
the
of medium.
original
Beadle
illustration,
reasons.
ot first
to occur.
Laboratory
stand
permits
is not
liters
Busch
accompanying
following
increases
is apprwched,
begins
3-i/2
2G-714).--Adolphus
MO.
tube.
weight
branching
by
have
designed
a year’s
use
dry
germination
when
A
of growth
The
culture.
As maximum
allows
rolls
used.
more
We
than
easy cleaning
os convenient
l5/17
Charlotte
precise
inside
Street,
diameters
Inside
diomerer
8mm
/
--f---’
2.5cm
4
to
tubing.
make
numbers
of tubes
was the
make
the use of specially
The
the
tubes
straight
tube.
diation
ond
chimney
the
Tubes
in the
of glass
racks
sterilized
may
*,’
30cm
also
allows
have
also
been
of
the
these
hove
diameters
may
found
convenient
be obtained
by
when
dealing
feet,
small
unnecessary.
gloss
blobs
attached
to the
and
IO mls
of sterile
medium
access
constructed
tube
oven
for
is undesirable.
point
toking
with
4
)I+-#cm+;
os o convenient
easy
12 mm
\x---*
wool
rolls,
which
we
in o dry
be used
ends
middle
cotton
feature
5mm
feet
for
samples
chimneys
These
obtaining
at
tubes
end
have
been
the
University
and
overheads.
for
of
or
use
found
of
the
for
large
tube;
is pipetted
sampler
of mycelium
one
sides
osking
with
through
conidio.
conidia
The
from
in situations
to be slightly
these
all
ports
where
less
coniconven-
to handle.
The
The
of
provision
constructed
plugged
The
form
of the
ient
are
chimney.
use of
extra
An
diameter
-
fy
I+4cm --)A
os we hove
specified
specially
selected
I1
Inside
Gloss
cost
gloss
West
cost
Mains
of these
tubes
about
9d and
Rood,
Emerson,
5.
The
in the maintenance
Neurospora
crosso,
re-isolation
ofslime
ond
from
siderable
always
periods.
The
been
successful,
the
for
rest
the
following
us by
of the
o glassblower
cost
covered
in
labor
is approximately
--Institute
6 shillings.
of
Animal
Genetics,
9, Scotland.
Stocks
use of heterocaryonr
of slime
stocks
strains
by
the
Edinburgh
hyphol
recovered
have
os mode
o method
of
for
the
active
heterocaryons.
hyphal
growth
recovery
presumably
method.
of
of
rapidly
growth
essential
that
hove
habit
the slime
because
and
con
Neurospora
become
cases,
with
under
e.g.,
making
of such
heterocoryonr
ore in the minority.
under
slime
conditions
frequent
to their
maintenance.
been
produced
between
be maintained,
component
slime
nuclei
c~(1sso
inviable
phenotypes
in which
transfer
The heterocaryons
slime
strains
refrigeration,
for
by plating
conidia
In such
cases slime
and
conhas
has
not
been
1
The
heterocaryon
is inoculated
nutritional
element
by the slime
parent
sorbose
to
plats
the
which
and
the
standard
ore
when
colonies
ore
Fox,
the
to
filter
D. J.
is plated
and
of high
in part
media
growth
medium
requirement
time
some
may
spheroplosts
be expected
N.S.
Biology,
Rapid
F.
California
Institute
of
light
from
a Black
the
other
We
have
the
original
con
measured
be
make
found
it
This
principally
when
and
the
particular
Using
Laboratories,
this
table,
corrections
The University,
Laycock,
M.
D.
Boulter.
crosso
H. G.
The
separation
ore
acids,
it
with
numbers
large
from
this
after
50%
hove
of
and
gel
which
by
in
-
136,
to
work
may
incubation
out
gels
according
by
be expensive
time
several
Thurman
vividly,
This
resulting
will
to
the
Distillation
and
due
into
method
of
Ornstein
(Fox,
o Thunberg
Industries,
Thurman
and
Eastman
and
the
com-
o pole
blue
spectrophotometer.
Once
interference
the
localized
from
extinction
at
Z.
to construct
310,
280
384,
1941).
o Nomogram
observed
E280
and
absorption
the
from
E2@
any errors
mg.
protein
Boulter,
peaks.--The
to the
arising
by the
is in fact
due
conversion
electrophoresis
technique
tube.
Davis
Kodak
and
Co.,
Biochem.
four
ond
1962)
J.,X,
Science
The
gel
of
iroenzymes.
results
starch
adenine
d) ease
Polyacrylamide
(Omstein
(Tsoo,
using
advantages
electrophoresis
min.
os opposed
to E-20
hr.
volume
of incubation
medium
nicotinamide
bonds,
least
similar
over
Botanical
homogenates
electrophoresis.
time
is 40
only
a small
for
at
reports
gel
latter
defined
Trao
that
contain
communication
the
Hartley
demonstrated
crosso
requirement
clearly
fit
is
This
it has been
under
ultra-violet
Biochem.
when
elution
columns
when
cases
to the
thus eliminating
conversion
to
gel
1962)
electrophoresis
and require
to the
in sharp,
readily
Products
Boulter
are a)
ore smaller
the
usually
to meowre
some
starch
42,
Neurospora
electro-
studies
with
dehydrogenoses
b) polyacrylomide
gels
this
since
Fox,
mode
Using
Neurospora
California.
laborious
Christian,
of o
Grant,
establishing
In such
examination
converting
polyacrylamide
c) shorter
for
fractions,
of
Inasmuch
purification.
of
Preprinted
o table
been
of malic
polyocrylamide
for enzyme
using
starch,
carried
of
stand
chromatographic
in a spectrophotometer.
Owing
and
o pure
o U.S.P.H.S.
with
on S. P. 500
in pyrex
test tubes.
would
hove
given,
inherent
in direct
determined
by
very
fluoresce
is customary
(Worburg
in
components.
Pasadena,
curve.
visual
spectrophotometer.
phoresis.
tally
proteins
is measurable
ore contained
correction
and
of over
Liverpool.
Kblmark
isoenzymes
by
nucleic
figures
that
the
component
alone
acid
composition
proteins
V.,
dehydrogenase
The
in
dealing
Christian
protein
amino
port
become
elution
by rapid
sphero-
wool
supple-
osmotic
concentration,
the slime
component
procedure
con
on the
fractions
dark.
accurately
profitable,
in
equation.
The only
assumption
here
is that the extraneous
their
components.
time
be converted
to mg.
protein
E280 may ot ony future
or
corrected
for
in the
o Worburg-Christian
Warburg
Warburg-Christian
nucleic
acids
lamp
separated
any concentration
even
when
the fractions
compounds,
rnp and
value
of E280 that the
the variation
of aromatic
factor
U. V.
detect
absorbing
260
Glass
the eye con
is satisfactory
absorption
and
and ore widely
the protein-containing
both
Thigh
in only
position
of proteins
from
to measure
the E280 mp
procedure
color,
and
This method
results
os in
Technology,
customary
and
glass
Persistent
slime
growth
of the sphero-
further
frequently
ond
that
required
IO per cent
hyphae
component.
carried
GT6174,
The
heterocaryotic
to permit
oil
in the
with
by adding
by filtration
through
osmotic
concentration,
in liquid
media
if os is present
Grant,
locali-
of
slime
as this
hove
lacking
supplemented
obtained
is removed
of standard
ploting,
chromatogmphic
ore few
in number
possible
to localize
concentration,
consists
of the
tested
eluotes.
ponents
found
osmotic
advantageous
before
so far
by on
of
D.
liquid
Hyphol
on agor
It is sometimes
produce
described
thot
Boulter,
in
on
growth
plates.
)--Division
of proteins
the
a second
(Supported
GM-0965.
in such
heterocaryons
t-es
from
heterocoryon.
zation
from
Caution:
os pure os strains
behovioaifferent
medium
composition.
to sotisfy
and
slime.
N. 1. H.
in
of spheroplosts
picked
a liquid
Growth
slime
necessary,
suspension,
persistent
into
by the hyphol
parent
of the heterocoryon
but
one.
High
osmotic
concentration
is ordinarily
medium.
largely
suspension
mented,
plost
required
if it has
of
gel
dinucleotide,
incubation
electrophoresis
onaerobiwas
Davis,
Disc Electrophoresis,
with
the modification
introduced
29,
1963).
from
to