Download RESEARCH NOTES Davis, R.H.

RESEARCH
Davis,
R.H.
mutants
Lethality
of
associated
with
Neurosporo
o factor
from
NOTES
orginine
In
wild
lacking
type.
yielded
ratios
after
factor,
isolation
derived
org-I2
mutant.
We
citrulline,
of
have
and
probably
2 wild:
to medium
from
the
been
able
ornithine,
activity
“lethal
org-12.
Another
absolute
ot
not
been
able
UM-300.
were
consistent
o normal
to show
by disappearance
from
of
It is not
concentrations
yet
to uptake
I should
or other
mutants,
on
Emerson,
S.
of
genetic
Genetic
isolations
characters
from
are
characters
of the
and
UM-300
if
too
miserable
recover
strains
hyphal
nature
and
Genetics,
to our
was
in
stock
able
to
of derepression.
analyzed
press,
the
pathway
of wild
rate
system
th an
and
extent
latter
UM-300.
is the
l/4
rate
wild
of elevation
The
double
“lethal
of
could
destruction).
type;
mechanism
per ml
mutant,
” progeny
not
be
arginine
this
of arginine
an arginine
concentration
be noted
thot
I mg orginine
is
ornithine
I low
arginine
normal
little
of arginine,
The
exogenously-provided
or o rapid
in our
very
that another
with
the
basis of high
of I high to
gives
of
regularly
grew
a low level
activity.
endogenously-synthesized
UM-300
73a
suggested
associated
is denoted
arg-12
mutant
and
Davis
crosses
type
but
ratio
(arg-m
or destruction)
mutants
1963),
germinate,
described
t o on
UM-300
transport
in
Neurospora
(arg-12:
On the
segregation
and
effects
thot
loss
system
UM-300
possible,
in
We
measured
was
mycelio
hove
when
is deficient,
medium
will
of
the
slime
appreciate
osmotic
-unknown,
without
OS,
fz, and
-hyphal
growth:
with
persistent
plasmodioid
(OS,
From
slime
publication
only
and
support
test
the
the
cultures
it separated
phenotypes.
for,
the
(see
Pub.
cleanly
IR,
which
was
mutant),
and
has extremely
by
slime
flows
wild
type.
with
arginine
were
between
(Proc.
XI
Because
to
cite
Int.
Cong.
more
data
this
note
Botany,
supplied
Neurospora
to the
Fungal
Information
1962)
little
with
was
Conknown
parental
in
spontaneous
poor
surface
instead
of germ
the
germination
growth
tubes
in
inherited
The
parent
(sg,
of slime),
linkage
Arcospore
habit7
but
of the
genes
irradiated
write
University
that
three
independently
of the slime
phenotype.
present
Neither
in
studied
of
together
It is now known
persistent
expression
group
Stadler
above.)
950,
internal
relationships
wishing
of slime
Center
acid
o normal
genetic
permission.--Deportment
time
the
experiences
investigators
NAS-NRC
germinate
does
with
ference,
linkage
than
amino
comparable
thot
Stock
that
other
poorly
had
ond
Genetics
o morphological
heat activation,
sg usually
forms
for
except
with
hyphal
yet i nsufficient
more
have
only
case reported
by D.
R.
with
the one described
be cited
At
the
strains
(A recent
similarities
slime
phenotype
orginine;
workers
to exchange
note
to concentrate
arginine,
since
some units
os external
concentrations.
concentrates
I should
the
inability
in the
is for
other
crassa.
are:
is scheduled
of
transcarbamylase
ml.
The progeny
for the
“Ietholity”when
arginine
imperfect
whether
soon,
and that
Michigan.
(fz
, linkage
group
unknown,
germinates
carrying
class
the
hypothesis
by
transport
which
heterocaryons
essential
to,
involved
isolates
the
and
to know
Neurosporo
basis
the
slower
and other
mutants
of this type.
vol.
I, p. 52) appears
to hove
variant
characters
the
is ornithine,
interested
be available
oil
UM-300
has o complete
hove
not been
measured
specific
for more
recent
results,
of Michigan,
Ann Arbor,
--fuzzy
group
of an
on analysis
arg-12.
be
UM-300
Genetics,
in
(through
medium
whether
arginine
how
the
is much
mutants
carrying
These
results
suggest
that
is not the case.
It should
UM-300,
clear
of
is it known
will
the
the medium.
destruction
growth
mutants
uptake
such
was
for
mutant
(because
that
cross
of o strain
with
Thwaites,
by way
x 74o
concentration
be concentrated
added
to
that
rapid
crosses
and
lost
of
ornithine
stock
used is abnormal
in having
o high
ornithine
transcorbamylase
responsible
in
could
regard
factor
The
levels,
of o 7%
The
observed
crossed
to
These
results
”
course
pgm arginine
per
was responsible
arginine
progeny
observed.
maintained
the
low
” category
UM-300,
when
200
parent,
of
the
was
I “lethal.
containing
wild
type
to show
that
the 73a
and also
in having
o consequence
transcarbamylase,
I arg-12:
the
eventually
change
some ---os fz
phenotypes.
of such
in
a
--No.
sg isolates
it has been
possible
by vegetative
selection
to
No genetic
difference
has yet been
established
between
A fuller
account
with
descriptions
of the unit
fz sg isolates.
3, Vol.
34
(1963/64)
of
Genetico.
(Supported
in port
by
on