SPA1: A New Genetic Locus Involved in Phytochrome A
... these genes encode negative regulators of light signaling that normally are active in the dark and inactivated by light (McNellis et al., 1994; Misera et al., 1994). However, recently, dominant mutants at two apparently novel loci have been described as well (Kim et al., 1996). Because mutations at ...
... these genes encode negative regulators of light signaling that normally are active in the dark and inactivated by light (McNellis et al., 1994; Misera et al., 1994). However, recently, dominant mutants at two apparently novel loci have been described as well (Kim et al., 1996). Because mutations at ...
Analysis of large and small colony L5178Y tk−/− mouse lymphoma
... observed in the many mutants analyzed, suggesting that loss of the entire 77t7b allele is not uncommon in tkr1' mutants. The molecular distinction between large and small colony mutants was not clear, since 71fc7b appeared to be completely lost in many mutants in both size classes. Cancer is a genet ...
... observed in the many mutants analyzed, suggesting that loss of the entire 77t7b allele is not uncommon in tkr1' mutants. The molecular distinction between large and small colony mutants was not clear, since 71fc7b appeared to be completely lost in many mutants in both size classes. Cancer is a genet ...
Novel p53 mutants selected in BRCA
... suppress transformation of primary rat embryo ®broblasts (REFs), perhaps by virtue of compromised ability to transactivate Bax or other p53 target genes, but also demonstrates a modest gain of function transforming phenotype (Crook et al., 1994; Rowan et al., 1996). Interestingly, mice null for Bax ...
... suppress transformation of primary rat embryo ®broblasts (REFs), perhaps by virtue of compromised ability to transactivate Bax or other p53 target genes, but also demonstrates a modest gain of function transforming phenotype (Crook et al., 1994; Rowan et al., 1996). Interestingly, mice null for Bax ...
SCARFACE in plant vein patterning - Development
... (Lincoln et al., 1990), axr2-1 (Timpte et al., 1992), and axr4 −2 (Hobbie and Estelle, 1995). From crosses of sfc-1 to aux1-7 and axr13, we identified F3 families in which all individuals (except the sfc-1 homozygotes) were resistant to 2×10−7 M 2,4-D. All seedlings within these families were presum ...
... (Lincoln et al., 1990), axr2-1 (Timpte et al., 1992), and axr4 −2 (Hobbie and Estelle, 1995). From crosses of sfc-1 to aux1-7 and axr13, we identified F3 families in which all individuals (except the sfc-1 homozygotes) were resistant to 2×10−7 M 2,4-D. All seedlings within these families were presum ...
Two Classes of sir3 Mutants Enhance the sir1
... were rescued from their chromosomal locations by standard methods of gap repair (Rothstein 1991). The gap-repaired plasmids that rescued the sir3-eso mutants were made by introducing pJR273, digested with BamHI and ClaI, into strains LPY238 and LPY222 for pLP465 and pLP468, respectively; and from pL ...
... were rescued from their chromosomal locations by standard methods of gap repair (Rothstein 1991). The gap-repaired plasmids that rescued the sir3-eso mutants were made by introducing pJR273, digested with BamHI and ClaI, into strains LPY238 and LPY222 for pLP465 and pLP468, respectively; and from pL ...
i A Thesis Entitled A Visual Screen for Centriolar Mutants in
... in the next section, then migrate away from the centriole leaving a unique cytoplasmic cilium in its wake (Enjolras et al., 2012; Basiri et al., 2014; Malicki and Avidor-Reiss, 2014). Shortly after the cilium begins to elongate, an unusual centriolar precursor called the proximal centriole-like (PCL ...
... in the next section, then migrate away from the centriole leaving a unique cytoplasmic cilium in its wake (Enjolras et al., 2012; Basiri et al., 2014; Malicki and Avidor-Reiss, 2014). Shortly after the cilium begins to elongate, an unusual centriolar precursor called the proximal centriole-like (PCL ...
Cell Death and Unbalanced Growth` in Neurospora
... Mutant strains of the ascomycete Neurospora crassa can be divided into two major types when conidiospores are incubated in a minimal medium. The first type will survive for relatively long periods on an agar plate containing minimal medium and will then respond to the addition of its growth requirem ...
... Mutant strains of the ascomycete Neurospora crassa can be divided into two major types when conidiospores are incubated in a minimal medium. The first type will survive for relatively long periods on an agar plate containing minimal medium and will then respond to the addition of its growth requirem ...
Journal of Bacteriology
... transcriptional level. Growth of PCL1445 at low temperature resulted in an increased level of putisolvins, and mutant analyses showed that this requires dnaK and dnaJ but not grpE. In addition, putisolvin biosynthesis of PCL1445 was found to be dependent on the GacA/GacS two-component signaling syst ...
... transcriptional level. Growth of PCL1445 at low temperature resulted in an increased level of putisolvins, and mutant analyses showed that this requires dnaK and dnaJ but not grpE. In addition, putisolvin biosynthesis of PCL1445 was found to be dependent on the GacA/GacS two-component signaling syst ...
Canadian journal of Microbiology
... cyanide, and siderophores, that may contribute to its biocontrol ability. In the gacS mutant background, generation of these products was markedly reduced or delayed with the exception of siderophores, which were elevated. Not surprisingly, this mutant was unable to protect canola from disease incit ...
... cyanide, and siderophores, that may contribute to its biocontrol ability. In the gacS mutant background, generation of these products was markedly reduced or delayed with the exception of siderophores, which were elevated. Not surprisingly, this mutant was unable to protect canola from disease incit ...
THE GENETICS OF CAENORΉABDITIS ELEGANS
... Mutants with a dominant effect are rare in C. elegans and the vast majority of the mutants found are recessive. These emerge in the F2 progeny, from the segregation of any F 1 heterozygotes. By this stage, the plate, initiated by a single parent, contains a very large number of individuals (up to 10 ...
... Mutants with a dominant effect are rare in C. elegans and the vast majority of the mutants found are recessive. These emerge in the F2 progeny, from the segregation of any F 1 heterozygotes. By this stage, the plate, initiated by a single parent, contains a very large number of individuals (up to 10 ...
The genetics of Caenorhabditis elegans.
... In most of the experiments, mutants have come from the clones produced by mutagenized adults. Although the F, progeny are heterozygous for induced mutations, a detectable fraction are abnormal in appearance or movement. Such variants have been picked with the intention of isolating dominant or semid ...
... In most of the experiments, mutants have come from the clones produced by mutagenized adults. Although the F, progeny are heterozygous for induced mutations, a detectable fraction are abnormal in appearance or movement. Such variants have been picked with the intention of isolating dominant or semid ...
A NEW TAIL-SHORT MUTATION IN THE MOUSE
... A large breeding colony of normal C females mated to normal A males was maintained for production purposes at the institute. Data from 329 females, involving 11,104 offspring gives a litter mean of 7.74. No genetically abnormal-tailed Fi mice were observed; although two independently different spont ...
... A large breeding colony of normal C females mated to normal A males was maintained for production purposes at the institute. Data from 329 females, involving 11,104 offspring gives a litter mean of 7.74. No genetically abnormal-tailed Fi mice were observed; although two independently different spont ...
hirota - Genetics
... Among mutants originally classified as galT mutants, there are some which are not complemented by F gal with deletions in the galK cistron and which map in galK (Figure 1). For instance, W3101 ( g a l T - I ) and W3991 ( g a l T - l ) map at different sites on galK. It is known that galK mutants ten ...
... Among mutants originally classified as galT mutants, there are some which are not complemented by F gal with deletions in the galK cistron and which map in galK (Figure 1). For instance, W3101 ( g a l T - I ) and W3991 ( g a l T - l ) map at different sites on galK. It is known that galK mutants ten ...
Anaerobic Growth of Escherichia coli K12 with
... were isolated after enriching for strains lacking the ability to use fumarate as the terminal electron acceptor unless supplied with supplements of 2-succinylbenzoate (SB) or 1,4dihydroxy-2-naphthoate (DHN).In addition, a partially characterized mutant (JRG860, S5 of Lambden & Guest, 1976) and the t ...
... were isolated after enriching for strains lacking the ability to use fumarate as the terminal electron acceptor unless supplied with supplements of 2-succinylbenzoate (SB) or 1,4dihydroxy-2-naphthoate (DHN).In addition, a partially characterized mutant (JRG860, S5 of Lambden & Guest, 1976) and the t ...
Heterozygote Advantage: The Effect of Artificial Selection in
... negative pleiotropic effects and consequently overall detrimental impacts on heterozygous fitness in a wild population. As examples in pets, lack of a tail as in Manx cats or hairlessness as in Mexican hairless dogs are artificially selected for but presumably would be selected against in heterozygo ...
... negative pleiotropic effects and consequently overall detrimental impacts on heterozygous fitness in a wild population. As examples in pets, lack of a tail as in Manx cats or hairlessness as in Mexican hairless dogs are artificially selected for but presumably would be selected against in heterozygo ...
Five components of the ethylene-response pathway
... mutants, and ein5 to different concentrations of the ethylene precursor ACC were compared (Fig. 1B). In the absence of the hormone, the mutant plants were indistinguishable from wild type. Conversely, at both high and low concentrations of the hormone, the mutant seedlings showed a significant degre ...
... mutants, and ein5 to different concentrations of the ethylene precursor ACC were compared (Fig. 1B). In the absence of the hormone, the mutant plants were indistinguishable from wild type. Conversely, at both high and low concentrations of the hormone, the mutant seedlings showed a significant degre ...
The Mutant of sll1961, Which Encodes a Putative
... Many cyanobacterial mutants with different photosystem stoichiometry from that of the wild type were reported (Wilde et al., 1995, 2001; Mann et al., 2000; Shen et al., 2002; Kufryk and Vermaas, 2003; Yu et al., 2003; Wang et al., 2004). The deletion of genes encoding the subunits of PSI or PSII may ...
... Many cyanobacterial mutants with different photosystem stoichiometry from that of the wild type were reported (Wilde et al., 1995, 2001; Mann et al., 2000; Shen et al., 2002; Kufryk and Vermaas, 2003; Yu et al., 2003; Wang et al., 2004). The deletion of genes encoding the subunits of PSI or PSII may ...
The axr4 auxin-resistant mutants of Arabidopsis thaliana define a
... screened 8100 T-DNA transformed lines of ecotype Wassilewskija (Feldmann, 1991) in pools of 100 families for seedlings able to elongate roots on agar containing 1 x 10.7 M 2,4-dichlorophenoxyacetic acid (2,4-D) (for details see Experimental procedures). Growth of wild-type seedling roots was almost ...
... screened 8100 T-DNA transformed lines of ecotype Wassilewskija (Feldmann, 1991) in pools of 100 families for seedlings able to elongate roots on agar containing 1 x 10.7 M 2,4-dichlorophenoxyacetic acid (2,4-D) (for details see Experimental procedures). Growth of wild-type seedling roots was almost ...
A genetic screen in zebrafish identifies the mutants
... generate a collection of over 400 zebrafish lines that are mutated in 315 recessive embryonic lethal genes. The gene bearing the mutagenic insert has been cloned for each line and we estimate that we have achieved ~22% saturation (Amsterdam et al., 1999). As many human genetic diseases are attribute ...
... generate a collection of over 400 zebrafish lines that are mutated in 315 recessive embryonic lethal genes. The gene bearing the mutagenic insert has been cloned for each line and we estimate that we have achieved ~22% saturation (Amsterdam et al., 1999). As many human genetic diseases are attribute ...
Transductional Analysis of Arginineless Mutants in Proteus rnirabilis
... treated with lysates prepared on homologous and wild-type bacteria respectively were included. Results were read after 48 hr. Selection of transductants. Wild-type transductants were selected on minimal medium and auxotrophic donor-type transductants on appropriately supplemented minimal medium (Len ...
... treated with lysates prepared on homologous and wild-type bacteria respectively were included. Results were read after 48 hr. Selection of transductants. Wild-type transductants were selected on minimal medium and auxotrophic donor-type transductants on appropriately supplemented minimal medium (Len ...
Mutations of genes in synthesis of the
... phs4, respectively, of which two mutant alleles were identified in phs2 (phs2-1 and phs2-2) and phs4 (phs4-1 and phs4-2; see Table 1). In addition, we later identified another two phs3 alleles by the zebra phenotype (see below). Molecular cloning of PHS1 through PHS4 genes Genetic analysis suggested ...
... phs4, respectively, of which two mutant alleles were identified in phs2 (phs2-1 and phs2-2) and phs4 (phs4-1 and phs4-2; see Table 1). In addition, we later identified another two phs3 alleles by the zebra phenotype (see below). Molecular cloning of PHS1 through PHS4 genes Genetic analysis suggested ...
Käfer, E. and D. Luk
... Results for UV, mitomycin C (MC) and HIS are summarized in Table 1. All new mutants were found to be cross-sensitive to some mutagenic agents and are, therefore, likely to be defective in DNA repair rather than uptake or metabolism of MMS. Most of the mutants listed in Tables 1 and 2 have been induc ...
... Results for UV, mitomycin C (MC) and HIS are summarized in Table 1. All new mutants were found to be cross-sensitive to some mutagenic agents and are, therefore, likely to be defective in DNA repair rather than uptake or metabolism of MMS. Most of the mutants listed in Tables 1 and 2 have been induc ...
View - OhioLINK Electronic Theses and Dissertations Center
... seven to nine stem cells referred to as the hub (de Cuevas and Matunis, 2011). Each stem cell asymmetrically divides to form one daughter cell, or spermatagonium, and one stem cell, keeping the hub replenished at all times (Kiger et al., 2001). The spermatagonium undergoes four mitotic divisions wit ...
... seven to nine stem cells referred to as the hub (de Cuevas and Matunis, 2011). Each stem cell asymmetrically divides to form one daughter cell, or spermatagonium, and one stem cell, keeping the hub replenished at all times (Kiger et al., 2001). The spermatagonium undergoes four mitotic divisions wit ...
Functional cooperation between the non-paralogous
... lumbar region than at the cervical and the thoracic level (Barone, 1986), (ii) all lumbar vertebrae are very similar to each other, lateral and spinous processes (the more useful features for vertebral rank identification) having a smooth gradient of size and shape from the first lumbar vertebra to ...
... lumbar region than at the cervical and the thoracic level (Barone, 1986), (ii) all lumbar vertebrae are very similar to each other, lateral and spinous processes (the more useful features for vertebral rank identification) having a smooth gradient of size and shape from the first lumbar vertebra to ...