Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Discovery and development of antiandrogens wikipedia , lookup
Toxicodynamics wikipedia , lookup
Discovery and development of angiotensin receptor blockers wikipedia , lookup
Drug design wikipedia , lookup
Cannabinoid receptor antagonist wikipedia , lookup
NK1 receptor antagonist wikipedia , lookup
NMDA receptor wikipedia , lookup
Psychopharmacology wikipedia , lookup
Neuropsychopharmacology wikipedia , lookup
Reminder: Henry Lester’s “office” hours outside the Red Door Mon, 1:15-2 PM, Fri 1:15-2 PM Bi 150 Lecture 7 Monday, October 12, 2015 Postsynaptic acetylcholine receptors; Synaptic transmission is a δ-function in time; Channel blockers Chapters 9, 12 1 Timing of synaptic events “Synaptic delay”, between the peak of the action potential and the start of transmitter release, is ~ 0.5 ms. Delay between the peak of the Ca2+ current and the beginning of the EPSP is ~ 0.2 ms (more at lower temperature). Most of the “synaptic delay” is caused in opening of Ca2+ channels during the action potential. mV The size and timing of the EPSP’s can be modulated by prolonging the action potential. Figure 12-1 2 Endplate Potential is not Regenerative (contrast to Action Potential) The current source for the epp is restricted to the endplate, so the size of the potential decays with distance from the endplate (space constant ~ 1 mm). Figure 9-5 3 electrical transmission in axons: electric field open closed Past lectures: V-gated Na+ channels V-gated K+ channels V-gated Ca2+ channels chemical transmission at synapses: [neurotransmitter] closed open Today: ACh-gated excitatory cation (Na+ / K+ / Ca2+) channels, Future: GABA and glycine-gated inhibitory anion (Cl- channels) Future: Glutamate-gated excitatory (Na+ / K+ / Ca2+) channels 4 From the previous lecture Many basic principles of chemical transmission were discovered at the neuromuscular junction (nerve-muscle synapse, endplate); acetylcholine is the transmitter. Figure 9-1 5 From previous lectures Fine structure of the nerve-muscle synapse 0.3 µm Incl. acetylcholinesterase ACh receptors Figure 9-1 6 The family of nicotinic ACh Receptors The mammalian genome contains 10 subunits (1 through 10) and 3 subunits (1 through 4), γ, δ, and ε subunits. Nicotinic receptors always contain at least two subunits. Muscle: (α1)2β1εδ Major brain ACh receptors: α4β2 (stoichiometry uncertain); (α7)5 Mouse Midbrain dopaminergic cells (coronal section, tyrosine hydroxylase stain) Substantia nigra pars compacta (controls motion); lost in Parkinson’s Disease A10 Ventral tegmental area (VTA, controls reward) Who introduced nicotine to European culture? Hint: which Holiday is today? Substantia nigra pars reticulata (GABAergic) These neurons express many types of nAChR subunits and combinations 7 Nicotinic Acetylcholine Receptor (Unwin, 2005) ~ 2200 amino acids in 5 chains (“subunits”), Binding region MW ~ 2.5 x 106 Membrane region Colored by secondary structure Colored by subunit (chain) Cytosolic region 8 The AChBP interfacial “aromatic box” occupied by nicotine (Sixma, 2004) Showing the cation-p interaction Y198 C2 W149 B Y93 A Y190 C1 non-W55 D (Muscle Nicotinic numbering) 9 How do Nicotinic Receptors Transduce Agonist Binding into Channel Gating? Swivel? acetylcholine or nicotine acetylcholine or nicotine Miyazawa & Unwin, Nature 2003 CLOSED Twist? Corringer et al., J Physiol 2010 OPEN In any case, the permeant ions experience a water-like environment CLOSED Hydrophobic Leu (Gate) OPEN Polar (Ser or Thr) –OH side chains (charge selectivity occurs in > 2 regions) 11 The Reversal Potential (Erev = EEPSP) for the synaptic potential (or current): not a True Equilibrium / Nernst Potential Membrane potential +80 ENa +60 +40 Most excitatory ligandgated channels allow flux of Na+ and K+ (sometimes Ca2+), and Erev ~ -5 mV. Positive to Erev, agonist pushes voltage more negative. outside GEPSP EEPSP (~ -5 mV) GK EK (-90 mV) cytosol = inside +20 -5 -20 At Erev , the agonist has little effect on membrane potential. -50 Resting -80 potential EK -100 Negative to Erev, agonist pushes voltage more positive. resting potential: K+ channels open Excitatory postsynaptic responses: Na+ / K+ channels open too EKGK + EEPSPGEPSP DV = GK + GEPSP Desensitization Occurs at Many Ligand-Gated Channels Free Energy unbound voltage-clamp trace from Xenopus oocyte expressing α4β2 nicotinic receptors agonist Bound states with increasing affinity “closed/ resting” “activated” Highest affinity “desensitized” Reaction Coordinate 106 channels nicotine 20 sec Time course of Postsynaptic Activation: Back to Feynman’s Idea A sensitive electronic ammeter a nicotinic acetylcholine receptor exposed to acetylcholine 5 pA = 104 ions/ms 20 ms dynamic range: 5 ms to 5 min 1 part in 108 14 How ”tight” is the gigaohm seal? 1. Electrically tight See next slide 1 mm Alberts 11-31 © Garland 15 How ”tight” is the gigaohm seal? 1. Electrically tight pipette wall R = rl/A R ~ 109 W; r = resistivity = 22 W-cm; l = length = 10 mm; A = area = 10 mm x t (thickness); Therefore t ~ 2 x 10-11 m, or less than 1 Å! t membrane 16 How ”tight” is the gigaohm seal? acetylcholine in the pipette opens channels in the pipette 2. Chemically tight acetylcholine outside the pipette opens channels outside the pipette The seal compartmentalizes molecules. Molecules outside the pipette do not mix with molecules inside the pipette 17 Neither Kandel nor Alberts describe Sigworth’s beautiful circuits sensitive electronic ammeter A Little Alberts 12-22D 18 © Garland from Lecture 2 Max Delbruck Carver Mead Richard Feynman “If you want to measure small, noisy signals, I have a Senior who can help” H. A. L 19 Fred Sigworth ‘74 and Apostol’s Clock Fred Sigworth’s Web page at Yale http://bbs.yale.edu/people/fred_sigworth.profile Ma 1A: http://www.amazon.com/Calculus-Vol-One-VariableIntroductionAlgebra/dp/0471000051/ref=sr_1_1?s=books&ie=UTF8&qid =1381358906&sr=1-1&keywords=apostol 20 Statistical analysis of single-molecule events channel opens now we synchronize artificially on the opening event n =1 0 At a synapse, the pulse of transmitter is ~ a d-function, and all postsynaptic channels open nearly synchronously Like Figure 9-10 21 Statistical analysis of single-molecule events n =1 0 Counts/bin Counts/bin counts/bin open time histogram time constant = Time time 1 k 21 closed time time const Time time 22 Molecular lifetimes from Chem 1b Lecture Series #5 (Heath) 23 Synaptic integration 1A. Molecular lifetimes (more in later lectures) Concentration of acetylcholine at nerve-muscle synapse (because of acetylcholinesterase, turnover time ~ 100 μs) high State 1 closed State 2 k21 all molecules begin here at t= 0 open 0 units: s-1 Number of open channels ms 24 Lidocaine, an example of a drug that blocks voltage-gated or ligand-gated channels Alkyl substituents: may adjust charge density of amine. Affects membrane permeation. Also affects binding to the receptor. Charged amine: may bind to charged groups or π electrons on the protein Amide: hydrolyzed to terminate drug action Alky groups affect both membrane permeation and receptor binding Aromatic: may bind to nonpolar groups on the receptor protein. Single-molecule recordings with a lidocaine analog 5 pA Acetylcholine only 20 ms Acetylcholine + blocking drug (QX-222, analog of lidocaine) 26 Drug interactions at the nicotinic acetylcholine receptor Some drugs compete with acetylcholine Some drugs bind on the axis ~ 100 Å (10 nm) 27 Model or scheme normal function State 1 State 2 k21 open closed units: M-1s-1 units: s-1 simple block k21 closed Functioning channel (1, 2) all molecules begin here at t= 0 open k23 = k+[Drug] drug blocked Drug-blocked channel (3) 28 current time constant = 1/k21 none time constant = 1/(k21+ k23) Functioning channel Drug-blocked channel 29 Stylized single-channel records for faster- and slow-binding blocking drugs Open (2) Closed (1) blocked (3) binds unbinds 30 Lidocaine blocks Na+ Channels from inside the cell inside Functioning channel “Trapped” or “Use-Dependent” Blocker lidocaine-H+ lidocaine-H+ lidocaine 31 Procainamide, a use-dependent blocker stimuli Action potentials fail impulses (voltage) channel population (currents) threshold pronounced block at brief intervals little block at long intervals 32 inside Functioning channel “Trapped” or “Use-Dependent” Blocker 33 Na+ channel blockers in medicine Local anesthetics Dental surgery (lidocaine) Sunburn medications Antiarrhythmics (heart) “use-dependent blocker” example: (procainamide) Antiepileptics / anticonvulsants (brain) “use-dependent blocker” (phenytoin = Dilantin® ) 34 Superfamilies of ligand-gated ion channels that are synaptic receptors A. ACh, Serotonin 5-HT3, GABA, (invert. GluCl, dopamine, tyrosine) receptor-channels Most ^ Figure 10-7 35 Reminder: Henry Lester’s “office” hours outside the Red Door Mon, 1:15-2 PM, Fri 1:15-2 PM End of Lecture 7 Interested in learning more about how drugs open and block ion channels? That’s part of neuropharmacology (Bi 155, Winter 2017) 36