Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Slide 1
Document related concepts
Zinc finger nuclease wikipedia , lookup
DNA vaccination wikipedia , lookup
Stem cell controversy wikipedia , lookup
Stem-cell niche wikipedia , lookup
Cre-Lox recombination wikipedia , lookup
Stem cell laws and policy in the United States wikipedia , lookup
Gene therapy wikipedia , lookup
Molecular cloning wikipedia , lookup
Somatic cell nuclear transfer wikipedia , lookup
Embryonic stem cell wikipedia , lookup
Cellular differentiation wikipedia , lookup
Gene prediction wikipedia , lookup
Community fingerprinting wikipedia , lookup
Induced pluripotent stem cell wikipedia , lookup
Hematopoietic stem cell wikipedia , lookup
Stem-cell therapy wikipedia , lookup
Therapeutic gene modulation wikipedia , lookup
Genetic engineering wikipedia , lookup
Epigenetics in stem-cell differentiation wikipedia , lookup
Genome editing wikipedia , lookup
Site-specific recombinase technology wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
Transcript
Lecture 20 Methods to produce transgenic animals What are Transgenic Organisms? Transgenic organisms are organisms that are injected with foreign DNA from another organism http://www.unc.edu/~unclng/cow.jpg How are they created? DNA Microinjection Retrovirusmediated gene transfer Embryonic stem cell-mediated gene transfer DNA Microinjection Direct microinjection of a chosen gene construct from another member of the same species or from a different species, into the pronucleus of a fertilized ovum. Retrovirus-mediated gene transfer Retroviruses are used as vectors Infects host cells Not all cells carry the retrovirus The retrovirus must integrate into the germ cells to work. Embryonic stem cell-mediated gene transfer Insertion of desired DNA sequence into an in vitro culture of embryonic stem (ES) cells. Stem cells can give rise to a complete organism. The cells are then incorporated into an embryo at the blastocyst stage of development.
