... culture of embryonic stem (ES) cells.
Stem cells can give rise to a complete organism.
The cells are then incorporated into an embryo at the
blastocyst stage of development.
Biological characterization and in vitro culture of human
... epithelium. While, spermatogenesis in rodents and primates, including humans, has
been considered dissimilar with important biological differences in the identity and
behavior of stem cells, recent studies from our laboratory and others recently
challenged this con ...
View Press Release
... squirrel (AGS) neural stem cells for research use and provides Neuronascent with license
fees and royalty income.
The Arctic ground squirrel is the only known mammal capable of lowering its body
temperature to below freezing during hibernation. Scientists believe that this tolerance
makes AGS cells ...
Cell Specialization and Levels of Organization
... • Embryonic stem cells are found in embryos
that are less than a week old
• In the lab these totipotent stem cells are able
to keep dividing for up to a year without
– Can make any one of the 300 cell types found in
an adult human
Techniques for Obtaining Stem Cells: Article 2 Therapeutic Cloning
... Therapeutic cloning has been negatively associated with the idea of human reproductive cloning
but the majority of scientists do not support the reproductive uses of this procedure in humans.
A somatic cell is any cell in the body not involved in gamete production or the gametes. Skin
cells, bone ce ...
new biotechnology PowerPoint
... animals to create the
antibodies so less ethical
• Antibodies can be ‘tailor
made’ to lock onto a
massive range of
different antigens like
pathogens and proteins
that cause allergic
Multicellular Organisms - Thomas A. Stewart Secondary School
... All multicellular organisms
begin as stem cells. These are
unspecialized cells capable of
developing into many different
types of cell.
Stem cells found in embryos are
called embryonic stem cells
and develop into all the different
types of cell in the body.
In the earliest stages of
development, ste ...
“Science will soon create the perfect human
... human being; however, it does have its hurdles to clear, suggesting that scientific perfection may
not be in the near future.
Aldous Huxley’s novel Brave New World prophecies the creation of the perfect human.
A dystopian novel, Huxley’s prediction depicts a very real future. In the novel, humans ar ...
Press Release - MWM
... Already twenty years ago, researchers showed that primordial germ cells (PGCs) could be induced
into pluripotency by the mere modulation of the culture conditions. Recently, Hans Schöler’s
research group succeeded in converting adult germline stem cells (GSCs) from mouse testicular cells
into plurip ...
... Buzgo M., Greplova J., Soural M., Bezdekova D. , Lukasova V. , Mickova A. , Lytvynets A. , Hlavac J. , Electrospun PVAPEG-biotin Immunonanofibers with Controlled Decay (Poster at Nancon, 2012)
Bezděková D., Buzgo M., Lukášová V.: Plasmatic modification of PVA nanofibers to enhance adhesion and proli ...
Pancreatic cancer breakthrough: scientists turn cancer cells into
... A new research study has shown that pancreatic cancer cells can be coaxed to revert
back toward normal cells by introducing a protein called E47. E47 binds to specific DNA
sequences and controls genes involved in growth and differentiation. The research
provides hope for a new treatment approach for ...
Adult neural stem cells, which are commonly thought of as
... down very early during embryonic development. It’s almost as if the embryo is
planning for the future.”
The study was published on June 18 in Cell.
Adult stem cells are found in organ systems throughout the body. Most are
undifferentiated, meaning they have the potential to develop into several
Stem Cell Therapy for Post-Polio Syndrome - Post
... cord. Muscles such as the paraspinal or hip muscles, if damaged, can result in
more disability than more distant muscles, like the calf. So it may be possible
to have a positive impact on muscles at or above the hip, where they cause the
most disability if weakened. Regardless, there are several pro ...
I want to be the first person to use stem cells to help fix an eye.
... Psychology, Anatomy courses,
Embryology and Genetics.
I will obtain my bachelors of science.
In graduate studies I will take…
Masters in Science (that includes
specialized courses in all science
PhD in Science (specific to physics and/or
Stem Cell Line Glossary Adult stem cells: Also known as somatic
... Stem Cell Line Glossary
Adult stem cells:
Also known as somatic stem cells or tissue specific stem cells. Found in both juveniles and adults, these stem cells
are capable of dividing or self-renewing indefinitely and generate all the cell types of the organ from which they
A pr ...
Name: Date: Friday January 13th 2012 Biology Chapter 3 and 4 Test
... in diameter, and one maple tree has a trunk that is 3 metres in diameter. Which tree is older?
Explain your answer. [3 marks]
There are two cylinders of lateral meristem within their stems. The outer cylinder
produces cork to replace old epidermal cells. The inner cylinder produces phloem on the
Honors Biology Botany Lab Practical Review
... Monocot leaf: id as monocot, id vascular bundle, bulliform cells, guard cells, cuticle
Dicot leaf: id as dicot, id cuticle, palisade mesophyll, epidermis, stomata, guard cells,
Be able to id the following leaf environments: mesophytic, xerophytic, hydrophytic
Monocot stem: id as mon ...
Cellular Dynamics International, Inc. 525 Science Drive Madison, WI
... A recognized leader in the development and manufacture of cell therapies, Dr. Hei has published
extensively in the area of developing stem cells and cell-based therapies.
At Waisman Biomanufacturing, Dr. Hei was responsible for establishing a biotherapeutics and cell
therapy contract manufacturing f ...
Differentiation and containment of derived pancreatic beta cells
... States, according to the American Diabetes Association. Currently, the only permanent cure for T1D is a
pancreatic or islet transplant. With a large shortage of available donors, our work is progressing research
towards alternative therapies by differentiating induced pluripotent stem cells (iPSCs) ...
Stem Cells and Ethics
... Blood cells
Each cell type has a special
task and structure
Each cell has the same DNA,
but only a section of it is
Separation, functional activity measurements
... QUANTITATIVE (REAL-TIME) PCR (qPCR/qRT-PCR)
cells RNA isolation
RNA reverse transcription (RT-PCR) cDNA
cDNA polymerase chain reaction (PCR)
determination of quantity
(investigation of gene activation on protein level WB)
Sample preparation, probe labeling and hybridization for experiment-1
... In order to obtain the regulated expression of dominant negative mutant RhoG in Hela cell, RhoG17
cDNA was cloned into pcDNA4/TO vector for expression. Two petri dishes/construct (10 cm each)
of T-Rex-HeLa cells (Invitrogen) were transfected with Fugene (Roche) pcDNA4/TO vector or
vector with contai ...
Stem-cell niche refers to a microenvironment where stem cells are found, which interacts with stem cells to regulate cell fate. The word 'niche' can be in reference to the in vivo or in vitro stem-cell microenvironment. During embryonic development, various niche factors act on embryonic stem cells to alter gene expression, and induce their proliferation or differentiation for the development of the fetus. Within the human body, stem-cell niches maintain adult stem cells in a quiescent state, but after tissue injury, the surrounding micro-environment actively signals to stem cells to promote either self-renewal or differentiation to form new tissues. Several factors are important to regulate stem-cell characteristics within the niche: cell–cell interactions between stem cells, as well as interactions between stem cells and neighbouring differentiated cells, interactions between stem cells and adhesion molecules, extracellular matrix components, the oxygen tension, growth factors, cytokines, and the physicochemical nature of the environment including the pH, ionic strength (e.g. Ca2+ concentration) and metabolites, like ATP, are also important. The stem cells and niche may induce each other during development and reciprocally signal to maintain each other during adulthood.Scientists are studying the various components of the niche and trying to replicate the in vivo niche conditions in vitro. This is because for regenerative therapies, cell proliferation and differentiation must be controlled in flasks or plates, so that sufficient quantity of the proper cell type are produced prior to being introduced back into the patient for therapy.Human embryonic stem cells are often grown in fibroblastic growth factor-2 containing, fetal bovine serum supplemented media. They are grown on a feeder layer of cells, which is believed to be supportive in maintaining the pluripotent characteristics of embryonic stem cells. However, even these conditions may not truly mimic in vivo niche conditions.Adult stem cells remain in an undifferentiated state throughout adult life. However, when they are cultured in vitro, they often undergo an 'aging' process in which their morphology is changed and their proliferative capacity is decreased. It is believed that correct culturing conditions of adult stem cells needs to be improved so that adult stem cells can maintain their stemness over time.A Nature Insight review defines niche as follows:""Stem-cell populations are established in 'niches' — specific anatomic locations that regulate how they participate in tissue generation, maintenance and repair. The niche saves stem cells from depletion, while protecting the host from over-exuberant stem-cell proliferation. It constitutes a basic unit of tissue physiology, integrating signals that mediate the balanced response of stem cells to the needs of organisms. Yet the niche may also induce pathologies by imposing aberrant function on stem cells or other targets. The interplay between stem cells and their niche creates the dynamic system necessary for sustaining tissues, and for the ultimate design of stem-cell therapeutics ... The simple location of stem cells is not sufficient to define a niche. The niche must have both anatomic and functional dimensions""