Download ImmunO™ Rabbit, Anti-S19 Ribosomal Protein Catalog #: 63659 Lot

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Certificate of Analysis
ImmunO™
Rabbit, Anti-S19 Ribosomal Protein
Catalog #: 63659
Lot #: 6033F
Form:Lyophilized. The antibody (100 ug) is supplied in PBS with 1.0% BSA as a stabilizer and 0.1% Proclin as preservative.
Reconstitution:Reconstitute with 100 ul of distilled or de-ionized water.
Concentration:1 ug/ul
Preparation:Rabbits were immunized with S19 recombinant protein. The antibody was purified from rabbit serum by Protein G
affinity chromatography.
Applications:Immunohistochemistry
ELISA
Working
Dilution:1-5 ug/ml for Immunohistochemistry
0.5-2.0 ug/ml for ELISA
1-5 ug/ml Immunoblotting
It is recommended that the individual lab obtain their own optimal dilution for their particular assay.
General
Information:The S19 ribosomal protein is composed of 145 amino acids and has a molecular weight of 16 kD. It is a
cross-linked homo-dimer from apoptosis cells. This protein shows monocyte chemotactic activity and is related to rheumatoid
arthritis.
Storage:Store at -20oC. After reconstitution, aliquot and store at -20oC.
References:1. Nishiura, H., et al. Monocyte chemotactic factor in rheumatoid arthritis synovial tissue. Probably a cross-linked
derivative of S19 ribosomal protein. J. Bio. Chem. 271(2): 878-882 (1996).
2. Yamamoto, T., et al. Molecular mechanisms to form leukocyte infiltration patterns distinct between synovial tissue and fluid of
rheumatoid arthritis. Seminars Thrombos. Hemost. 22(6): 507-511 (1996).
3. Horino, Kei, et al. A Monocyte Chemotactic Factor, S19 Ribosomal Protein Dimer in Phagocytic Clearance of Apoptotic Cells.
Lab Invest. 78: 603-617 (1998).
4. Draptchinskaia, Ntalia, et al. The gene encoding ribosomal protein S19 is mutated in Diamond-Blackfan anaemia. Nature
Genetics 21: 169-175 (1999).
5. Shrestha, Arjun, et al., Acquired Immune Response as a Consequence of the Macrophage-Dependent Apoptotic Cell
Clearance and Role of the Monocyte Chemotactic S19 Ribosomal Protein Dimer in this Connection. Laboratory Investigation
79(12): 1629-1642 (1999).
Approved by: Joseph Dietz, Ph.D
Quality Control Director
Control # R1205