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About I am studying for a M.Sc. Science Communication at Imperial College London. One of my courses was to design a science communication website. I have a blog (external link 1 = http://penblogsandbuildsawebsite.wordpress.com/) describing the construction process for this web page. Since I have a degree in Biological Sciences with honours Plant Science from the University of Edinburgh, genetic modification (GM) in plants seemed the logical choice. It is part of the reason I became interested in science communication. It is a controversial topic, which is yet again in the mainstream media. I wanted to design a website that reflected my thoughts on GM. I am not trying to present a definitive opinion on GM, simply reflect what I have been taught during my degree. I would also like to add an international dimension since I have lived in both the UK and USA. What is GM? All organisms have genes, made up of DNA. Genetic modification (GM) describes the process by which an organisms’ genetic material has been altered. On this site, I will focus on what that means within the plant kingdom. GM can be achieved through the insertion of genes. These genes can be from plants within the same species, or from different species. The ultimate goal is to have a plant with desirable traits. There are several techniques for GM, or plant transformation. Descriptions can be found on the “How are GM plants made?” <link> page. §When it was first used / discovered The first GM plant was created in 1983 [1]. It was a transgenic <definition> tobacco plant [1]. This was made possible after Watson, Crick, Franklin and James discovered that DNA was the smallest unit of life. Scientists began working with bacteria [2]. Several decades of research lead up to the first plant transformation [2]. [1] http://news.bbc.co.uk/1/hi/special_report/1999/02/99/food_under_the_micro scope/280868.stm [2] http://www.whoinventedit.net/who-invented-genetic-engineering.html §How it is different to cloning Cloning produces an identical copy to the original. This is different to GM which produces a plant with new genetic material. The techniques used are also different; cloning uses an adult cell which is placed in an ovum with the nucleus removed (definition). A small electrical charge is passed through which starts the cell cycle. How are GM plants made? §Information about the main techniques used in plant transformation: There are two main techniques for plant transformation: Biogun <link> Agrobacterium tumefaciens <link> I will explain these techniques in slightly more detail, but I will focus on A. tumefaciens. This is because it is the more efficient and elegant technique. It also has an interesting story rooted in plant pathology. §Bio gun The biogun is pretty much how it sounds. A solution containing genetic material (definition) is ‘shot’ at plant cells. The force of the action opens microscopic holes where the DNA can enter. §Agrobacterium tumefaciens This bacterium, in the wild, causes a plant disease called crown gall (definition). The clever bacterial mechanism is exploited for plant transformation. For more information, please follow the link to the A. tumefaciens page <link>. For both processes, this is by no means a definite event; most of the DNA will not enter the cell. Even if it does, only a fraction will be taken up into the plant genome (definition). Of those cells which take up the new DNA, only a small number will be viable. The DNA may enter at a point that disrupts a vital plant process or in an exon (definition) sequence. A. tumefaciens §More about the bacteria: §Diagram to show the bacterial cell §More about the disease it causes in nature (which is called Crown Gall disease) §Why it was selected for plant transformation Uses of GM plants §Where GM plants are used §Why use GM plants – link to current / future concerns §Crop resistance §Drought resistance §Benefits of GM to farmers §Statistics about disease crop losses §Benefits to consumers §Example of Golden Rice™ Where are GM plants used? §What countries allow GM plants to be grown §Stance of: UK, USA, EU §Links to pages that have the official information §I think it is important to have links where appropriate. Any good sci comm site should have links to sources as it helps give credibility to what is being said if you can see the original information. §What kind of products are on sale here that may be GM Concerns and benefits of GM §Concerns §Quote from Steve Jones’ BBC impartiality report §Benefits §Primary benefits §Secondary benefits §Tertiary benefits §This will link to the clone beef stories from a few years ago. There was some confusion over the difference and GM was brought into the debate under the umbrella of “Frankenfoods”