Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Immune system wikipedia , lookup
Lymphopoiesis wikipedia , lookup
Psychoneuroimmunology wikipedia , lookup
Adaptive immune system wikipedia , lookup
Molecular mimicry wikipedia , lookup
Monoclonal antibody wikipedia , lookup
Cancer immunotherapy wikipedia , lookup
Polyclonal B cell response wikipedia , lookup
Adoptive cell transfer wikipedia , lookup
Innate immune system wikipedia , lookup
98405 Dioxin and Host Susceptibility to Infection Vivek Mehta Mentor: Sastry Gollapudi Dioxin, an environmental pollutant, increases host susceptibility to infection, but little is known about the mechanisms by which this occurs. Recent research has identified a family of molecules known as the Toll-like receptors (TLR’s) that play a key role in pathogen recognition and initiation of inflammatory and immune response. The purpose of this study was to determine the effect dioxin might have on the expression of TLR’s and TLR signaling. A monocytic cell line (U937, ATCC) was cultured with various concentrations of Dioxin (1 ng/ml, 10 ng/ml, 50 ng/ml, 100 ng/ml) for 2 weeks. Cells were stained phycoerythrin (PE) labeled monoclonal antibodies against TLR 2, TLR 4 and isotype control antibody. Dioxin treated and control cells were stimulated with lipopolysaccharde (LPS, 10 ug/ml), an agent known to trigger cell activation via TLR 4. Control and activated cells were permeabilized and stained with PE labeled anti-TNF- antibody. 10,000 stained cells were acquired and analyzed by FACScan flow cytometer. TLR positive cells, TLR receptor density, as well as the percentage of TNF- producing cells were determined using cell quest software program. Dioxin, in a concentration-dependent manner, inhibited the expression of TLR 2 and TLR 4. Altered expression of TLR 4 was associated with a decreased production of TNF- These data suggest that dioxin may increase host susceptibility to infection by modulating TLR expression and TLR signaling.