Download MCQ- V-Semester 2015 - KLE College of Pharmacy

KLEU College of Pharmacy, Belagavi
B.Pharm V-Semester (RS2) (2015-16)
Pharmaceutical Biotechnology- Multiple Choice Questions
SI.
No.
Content
Answer
Unit- I/ Applied Microbial Technology
1.
2
3
4
5
6
7
8
9
10
11
12
Unicellular organism:
A)Yeast
C) Protozoa
Viruses are:
A) Parasite
C) Obligate intracellular parasite
B) Bacteria
D) Virus
B) Saprophite
D) Obligate intracellular
saprophite
Alcohol is produced form:
A) Yeast
C) Bacteria
B) Mold
D) Actinomycetes
The Gram stain and acid-fast stain are based on chemical properties of the:
A) Plasma membrane
B) Outer membrane
C) Cell walls
D) Periplasmic space
Primary screening of organic acid and organic
amine producing organisms identified by:
A) pH indicating dye
B) Crowded plate technique
C) Dilution method
D) Gradient plate technique
Microorganism isolation is:
A) Purification of culture
B) Introduction of inoculums
C) Separation of a single colony
D) To grow microorganisms on
surface
Streptomycin is produced from:
A) Streptomyces griseus
B) Micromonospora purpurea
C) Streptomyces aureofaciens
D) Streptomyces erythreus
Pencillin is produced from the organism:
A) Penicillium chrysogenum
C) Penicillium griseofulvin
Streptomycin is an:
A) Aminoglycoside
C) Quinolones
Actinomycetes are:
A) Eukaryotes
B) C) Quinolones
B) Cephalosporium acrimonium
D) Streptomyces venezuelae
B) Beta lactam antibiotics
D) Macrolide
C) Beta lactam antibiotics
D) Macrolide
The best medium for the production of Penicillin is
A) Nutrient agar
B) Corn steep liquor
C) Sulfite waste liquor
D) Whey
Penicillin is recovered from the fermentation
broth by:
A) Precipitation
B) Solvent extraction
C) Chromatography
D) Adsorption on charcoal
13
14
15
16
17
18
19
Riboflavin is produced from:
A) Clostridium butyricum
B) Acetobacter suboxydans
C) Saccharomyces cerevisae
D) Torula lactosa
Penicillin is discovered by:
A) Robert koch
B) Edward jenner
C) Louis pastuer
D) Alexander fleming’s
Vitamin c is produced by fermentation using the
microorganism:
A) Acetobacter suboxydans
B) Aerobacter aerogenes
C) Saccharomyces pombe
D) Saccharomyces cerevisiae
Penicillin is sterilized by:
A) Moist heat
C) Autoclave
Streptomycin is produced from:
A) Molds
C) Virus
Industrial production of streptomycin involves:
A) Anaerobic submerged technique
C) Anaerobic Surface culture
Highest yield of streptomycin requires:
B) Dry heat
D) Chemical agent
B) Protozoa
D) Actinomycetes
B) Aerobic submerged technique
D) Aerobic surface culture
A) Nitrogen source
C) Carbon source
20
21
22
B) Meat extract
D) Minerals
Proteolytic activity of Streptomyces griseous releases :
A) Ammonia
B) Carbon
C) Calcium
D) Glucose
Streptomycin is available as:
A) Sulphate form
B) Hydrochloride form
C) Sodium salt form
D) Base form
Industrial production of streptomycin involves:
A) Anaerobic submerged technique
C) Anaerobic Surface culture
23
Enzymes responsible for alcoholic fermentation:
B) Zymase
A) Ketolase
C) Peroxidase
24
B) Aerobic submerged
technique
D) Aerobic surface culture
D) Oxidase
Which type of spores are produced sexually?:
A) Conidiospores
B) Sporangiospores
C) Ascospores
D) Chalmidospores
25
26
27
28
In the citric acid production, the pH to be maintained in the fermentor is:
A) 3.5
B)5.0 to 6.0
C) 8.0 to 9.0
D) 1.0 to 6.0
Cobalt is added as precursor in the fermentation of :
A) Vitamin B 12
B) Vitamin C
C) Vitamin B 6
D) Vitamin D
The required temperature for the production of
citric acid is:
A) 10oC – 80oC
B) 30oC – 50oC
C) 20oC – 50oC
D) 25oC – 30oC
The by-product during streptomycin production is:
A) Vitamin A
B)Vitamin B2
C) Proline
29
β-lactum ring is present in:
A) Erythromycin Disk plate method
30
33
34
35
36
D) Lactic acid
The raw material for citric acid production is:
A) Corn
C) Starch
32
B) Streptomycin
C) Penicillin
D) Tetracyclines
Aspergillus niger is used generally for the production of:
B) Penicillin
A) Ethanol
C) Citric acid
31
D) Vitamin B 12
B) Molasses
D) Whey
Citric acid is produced in aerobic conditions by the fungi:
A) Aspergillus niger
B) Saccharomyces
C) Agaricus bisporus
D) Chrysogenum notatum
Phages are:
A) Bactria
B) Fungi
C) Protozoa
D) Virus
Citric acid is used as:
A) Acidulant
B) As an antioxidant
C) As preservative
D) Pickling agent
In the production of ethanol industrially the yeast used is:
A) K.pneumoniae
B) Kluyreromyces fragilis
D) Aspergillus niger
C) S. cerevisiae
Clostridium acetobutylicum is used for the production of:
B) Ethanol
A) Acetone – Butanol
C) Vitamin-B12
D) citric acid
37
38
39
40
41
42
43
44
45
Citric acid is used as:
A) Flavoring agent in food
C) As preservative
B) As an antioxidant
D) Pickling agent
The most common substrate used in distilleries for the production of ethanol is:
A) Molasses
B) Corn meal
C) Soya meal
D) Ground gram
Industrial Production of Vitamin-B12 is from:
A) Propionibacterium sps.
B) Pseudomonas sps.
C) Pseudomonas sps.+ Propionibacterium sps.
D) Candida sps
The penicillin produced in large scale submerged fermentation is:
A) Penicillin A
B) Penicillin G
C) Penicillin D
D) PAP
The pH to be maintained for the production of penicillin is:
A) 7.5
B) 6.5
C) 8.0
D) 5.0
Unit _II / Enzyme Technology
Support material used for immobilization of enzyme by adsorption method:
A) Cellulose
B) Gelatin
C)
D) Glutaraldehyde
Alginate
Advantage of immobilization of enzyme:
A) High rate of reaction
B) less stable
C) Continuous use
D) Low rate of reaction
Medium containing all nutrient s in excess but controlling one nutrient is referred
as :
A) Turbidostat
B) Chemostat
C) Continuous culture
D) batch culture
Methods used to get immobilized enzymes:
A) Adsorption
B) Absorption
C) Chemical reaction
D) Ion exchange
Unit –III/Bioprocess Technology
46
47
48
49
50
51
52
53
54
First Pasteur conducted fermentation experiments in:
A) Milk
C) Fruit juices
C) Fruit juices
D) Milk and fruit juices
Fixed bed fermentor is also known as:
A) Immobilized cell fermentor
B) Fed batch fermentor
C) Tower fermentor
D) Cyclone column fermentor
Tower fermentors are used for the production
of:
A) Enzymes
B) Beer
C) Antibiotic
D) Vitamins
Downstream processing involves:
A) Purification
B) extraction
C) Production
D) extraction and purification
Upstream processing involves:
A) Development & production
B) medium selection
C) Production
D) recovery of product
The capacity of laboratory fermentor is:
A) 12-15 lt
B) 2 lt
C) 500 lt
D) 10 lt
The overheating of fermentor during fermentation is controlled by
A) Cooling agent
B) Steam
C) Cool air
D) Air bubbling
In fermentor the top portion left without broth is called:
A) Shaft
B )Impeller
C) Sparger
D) Head space
The purification and recovery of product after fermentation is called:
A) Upstream processing
B) Downstream processing
C) Surface fermentation
D) Submerged fermentation
55
56
57
Production of desirable product in the fermentor
is called:
A) Upstream processing
B) Downstream processing
C) Surface fermentation
D) Submerged fermentation
Batch fermentation is also called:
A) Open system
B) Fed batch
C) Closed system
D) Continuous fed batch
Antifoam agent is
A) Silicon compound
B) Corn oil
B) Soyabean oil
C) All the above
Unit- IV/Genetic Engineering
58
59
Restriction endonuclease is employed for cutting:
A) A single stranded DNA
B) Double stranded DNA
C) RNA fragment Anaerobic Surface culture
D) mRNA culture
Restriction endonucleases are:
A) Used in genetic engineering for uniting two
DNA molecules
B) Used for in vitro DNA
synthesis
C) Present in mammalian cells for degeneration D) Synthesized by bacteria for
of DNA of dead cells
their defense
60
61
62
63
Structure of DNA was given:
A) Kornberg
B) Nirenberg
C) Watson and Crick
D) Holley and Nirenberg
The basic unit of a nucleic acid is
A) Pentose sugar
B) Nucleoid
C) Nucleoside
D) Nucleotide
The enzyme taking part in joining two ends of DNA is:
A) Ligase
B) Polymerase
C) Gyrase
D) Helicase
In callus culture, roots can be induced by the supply of:
A) Auxin and no cytokinin
B) Higher concentration of auxin
and lower concentration of
cytokinin
C) Higher concentration of cytokinin and lower D) Both auxin and cytokinin in
concentration of auxin
equal proportions
64
65
66
67
68
69
70
71
In double helix of DNA, the two DNA strands are:
A) Coiled around a common axis
B) Coiled around each other
C) Coiled differently
D) Coiled over protein sheath
It is now possible to breed plants and animals with desired characters through:
A) Genetic engineering
B) Chromosome engineering
C) Ikebana technique
D) Bonsai technique
Plasmids are vectors for gene cloning because they
A) Replicate freely outside bacterial cells
B) Self replicate in bacterial
cells
C) Can be multiplied in cultures
D) Can be multiplied in
laboratories using enzymes
DNA is composed of repeating units:
A) Ribonucleosides
B) Deoxyribonucleosides
C) Ribonucleotides
D)Deoxyribonucleotides
The gene transfer occurs by:
A) Transformation
B) Transduction
C) Conjugation
D) Cell fusion
Gene is segment of:
A) RNA
B) DNA
C) RNA or DNA
D) Both DNA and RNA
Bacterial plasmid contains:
A) RNA
B) RNA + protein
C) DNA
D) Photosynthetic structures
Choose the correct statement:
A) DNA is hereditary material
B) RNA is hereditary material
C) DNA is hereditary material but where it is
absent RNA can function as hereditary material
D) Both DNA and RNA are
hereditary materials
72
73
74
75
76
77
78
79
80
In recombinant DNA technology a plasmid vector is cleaved by:
A) Modified DNA ligase
B) A heated alkaline solution
C) The same enzyme that cleave the donor
DNA
D) The different enzyme other
than that cleave the donor DNA
The most common plasmid vector used in genetic engineering is:
A) PBR 328
B) PBR 322
C) PBR 325
D) PBR 330
Eco RI is an
A) Ligase
B) Polymerase
C) Restriction enzyme
D) Gyrase
Extranuclear genetic material is found in:
A) Plastid and nucleus
B) Mitochondria and plastids
C) Nucleus and cytoplasm
D) Mitochondria and nucleus
The molecular formulae of deoxyribose sugar and ribose sugar respectively are:
A) C5 H10 O4 and C5H10O6
B) C5 H10 O4 and C5H10O5
C) C5 H10 O5 and C5H10O4
D) C5 H10 O5 and C6H10O4
DNA differs from RNA in:
A) Presence of deoxyribose sugar
B) Presence of thymine base
C) Property of replication
D) All the above
RNA contains:
A) Hexose
C) Fructose
B) Ribose
D) Glucose
Similarity between DNA and RNA is that both have:
A) Similar sugars
C) Similar pyrimidines
B) Similar mode of replication
D) Polymers of nucleotides
A small, circular DNA molecule used as a vector to transmit foreign DNA is :
A) Plasmid
B) Liposome
C) Ring chromosome
D) Primer
81
82
83
84
85
86
87
88
89
The first drug produced using recombinant DNA technology was:
A) Streptokinase
B) Insulin
C) Erythropietin
D) Human growth hormone
The polymerase chain reaction is used to:
A) Create millions of copies of an interesting
piece of DNA
B) Make more copies of DNA
polymerase
C) Speed the rate of DNA replication in cells
D) Copy protein into RNA
Genetic engineering manipulates gene products at the level of:
A) The protein
B) Amino acids
C) DNA
D) RNA
A molecule that consists of a piece of DNA from one organism combined with the
DNA from a member of another species is called:
A) Transgenic DNA
B) Bioengineered DNA
C) Recombinant DNA
D) Restricted DNA
The functions of plasmid are:
A) DNA replication
B) Protein synthesis
B) Protein synthesis
D) Genetic engineering
Annealing in genetic engineering is:
A) Joining of DNA
B) Cutting of DNA
C) Joining of RNA
D) Slicing of RNA
Which of the following bacteria is not a source of Restriction endonuclease
A) Haemophilus influenzae
B) Escherichia coli
C) Agarobacterium tumefaciens
D) Bacillus amyloli
The first step in polymerase chain reaction (PCR) is
A) Denaturation
B) Primer extension
D) Annealing
E) Cooling
What is another name for a restriction enzyme?:
A) Vector
B) Plasmid
C) Restriction endonucleases
D) Restriction sequence
90
91
92
93
94
95
96
97
Unit-V/Biologicals and Biopharmaceuticals
In tissue / bacterial culture nutrients are sterilized through:
A) Water bath at 200° C
B) Dry air oven at 200° C
C) Dehumidifier
D) Autoclave
In tissue culture, callus can be induced to form shoot or root by altering the ratio of:
A) Auxin to cytokinin
B) Cytokinin to ethylene
C) Auxin to gibberellin
D) Gibberellin to cytokinin
Genetically engineered bacteria are being used in commercial production of:
A) Melatonin
B) Testosterone
C) Human insulin
D) Thyroxin
A totipotent cell means:
A) An undifferentiated cell capable of
developing into a system or entire plant
B) An undifferentiated cell
capable of developing into an
organ Testosterone
C) An undifferentiated cell capable of
developing into complete embryo
D) Cell which lacks
the capability differentiate into
an organ or system
Callus is
A) Tissue that forms embryo
B) An insoluble carbohydrate
C) Tissue that grows to form embryoid
D) Unorganized actively
dividing mass of cells
Development of shoot and root in tissue culture is determined by:
A) Cytokinin and auxin
B) Enzymes
C) Temperature
D) Plant nutrients
Raising of plants from a small tissue in culture is known as:
A) Macro production
C) Tissue culture
B) Micro propagation
D) Mass production
Which of the following is not tool of genetic engineering?:
A) Vectors
C) Foreign DNA
B) Enzymes
D) GMO
98
99
100
101
102
103
104
105
106
Industrially important Antibiotic producing organisms shall be isolated by:
A) Disk plate method
C) Direct plate method
B) Serial dilution method
D) Crowded plate method
Vaccines are prepared from killed microbes, they are
A) Inactivated (killed) vaccine
C) Autogenous vaccine
B) Attenuated vaccines
D) Toxoids
Vaccines prepared from toxins and chemicals are:
A) Cellular vaccines
C) Attenuated vaccines
B) Sub-cellular vaccines
D) Heterologous vaccines
Hybridoma technique was first discovered by:
A) Kohler and Milstein
C) Herelle
B) Robert Koch
D) Land Steiner
The capacity of a given strain of microbial species to produce disease is known as:
A) Pathogen
C) Infection
B) Virulence
D) Disease
Term vaccine was coined by:
A) Robert Koch
B) Needham
B) Pasteur
D) Land Steiner
The bacterial cells are at their metabolic peak during:
A) Lag phase
C) Stationary
B) Log
D) Decline
Toxins or enzymes which are not produced by streptococcus pyrogens:
A) Hyaluronidase
C) Hemolysin
B) Phosphate
D) Streptokinase
Attenuation means:
A) Killing of the bacteria (microorganism)
C) Inactivation of bacteria
B) More activating the bacteria
D) killing and inactivation of
bacteria
107
108
109
110
111
The method used to maintain a continuous culture:
A) Chemostat
C) Thermostat
B) Autostat
D) Chemostat and thermostat
The organisms which can grow both in presence and absence of oxygen:
A) Aerobes
C) Faculative anaerobes
B) Anaerobes
D) Strict aerobes
In which medium the hydridoma cells grow selectively?:
A) Polyethylene glycol
C) Hypoxanthine aminopterin
thyminine
B) phosphoribosyl transferase
D) Molasses
The enzymes which are commonly usedin genetic engineering are:
A) Exonuclease and ligase
C) Ligase and polymerase
B) Restriction endonuclease and polymerase
D) Restriction endonuclease and
ligase
A successful hybridoma was produced by fusing:
A) Plasma cells and plasmids
B) Plasma cells and myeloma cells
112
113
114
C) Myeloma cells and plasmids
D) Plasma cells and bacterial
cell
Plasmids are ideal vectors for gene cloning as:
A) They can be multiplied by culturing
Hyalauronidase
C) They can replicate freely
outside the bacterial cell
B) They can be multiplied in the laboratory
using enzymes
D) They are self replicating
within the bacterial cell of the
DNA
Self-replicating, small circular DNA molecules present in bacterial cell are known:
A) Plasmids
C) Plasmomeros
B) Cosmids
D) Plastids
Monoclonal antibodies are produced by:
A) Hybridoma technology
B) Biotechnology
C) Fermentation
Technology
D) Biosynthesis
115
Enzymes are chemically:
A) Lipids
B) Proteins
116
B) Polyclonal antibodies
B) Fungi
119
120
121
122
C) Proteins
D) Antigens
Phyto-pharmaceuticals are produced from:
A) Bacteria
118
D) Fats
Hybridoma technique is used for:
A) Monoclonal antibodies
117
C) Carbohydrates
C) Plants
D) Animals
Protoplast fusion involves:
A) Fusion of DNA
C) Fusion of cells without cell
wall
B) Fusion of nucleus
D) Fusion of Cells with cell wall
Enzymes used in the Protoplast isolation:
A) Pectinase and Cellulase
C) Amylase and pectinase
B) Catalase and Lipase
D) Zymase and Amylase
Bio-pharmaceuticlas are:
A) Proteins or nucleic acids
C) Amino acids or antibiotics
B) Lipids or carbohydrates
D) Amino acids or fats
Biopharmaceuticals currently produced by genetic engineering in either:
A) E coli or animal cell
C) Virus or animal cell
B) Plant or animal cell
D) E coli or virus
Erythropoietin is secreted by:
A) Cells in macula densa
C) Juxtaglomerular cells
B) Cells in the proximal tubules
D) Cells in peritubular capillary
bed
123
124
125
126
127
128
Unit- VI/Medicinal Plant Biotechnology and Animal Cell Technology
The enzymes required to obtain wall-free / naked protoplasts are:
A) Cellulase and proteinse
B) Cellulase and Pectinase
C) Cellulase and Amylase
D) Amylase and Pectinase
Polyethylene glycol is:
A) Fusogenic material
B) Electro fusion stimulant
C) Callus stimulant
D) differentiation stimulant
Plant tissue culture technique is a redefined
method of:
A) Vegetative propagation
B) Asexual reproduction
C) Hybridization
D) Selection
Somatic hybridization is achieved through:
A) Grafting
B) Protoplast fusion
C) Conjugation
D) Recombinant DNA technology
The first transgenic crop was:
A) Pea
B) Tobacco
C) Flax
D) Cotton
The technique of hybridoma formation is from:
A) Spleen cell – Myeloma cell
C) Myeloma cell – Myeloma cell
129
130
B) Spleen cell – Spleen cell
D) kidney cell - Myeloma cell
The transgenic animals are those which have:
A) Foreign DNA in some of their cells
B) Foreign DNA in all of
C) Foreign RNA in all of their cells
D) Foreign RNA in some of their
cells
Polyethylene glycol method is used for
A) Energy production from sewage
B) Gene transfer without a vector
C) Seedless fruit production
D) Biodiesel production
131
132
133
134
Which part would be most suitable for raising virus free plants for micropropagation
A) Meristem
B) Node
C) Bark
D) Vascular tissues
Transgenic animals are for the improvement in quality of
A) Milk
B) Meat
C) Egg
D) All the above
The first crop plant sequenced is,
A) Rice
B) Tobacco
C) Maize
D) Cotton
DNA finger printing was developed by
A) Francis Crick
B) Khorana
C)Alec Jeffery
D) James Watson
Unit-VII /Proteomics and Genomics
135
136
137
138
The proteome is
A) only useful to be studied in conjunction with
the phenomena
B)refers to the entire complement
of proteins
C) is what functional genomics is primarily
interested in understanding
D) is now most commonly
studied using RNA microarrays
A Map Unit refers to
A) the relative distance between genes on a
chromosome
B) the chromosomes that
exchange parts during meiosis
C) the percentage of recombination
D) A and C
Genetics is the science of
A) Genes
B) Heredity
C) the variation of organisms
D) genes, heredity & the variation
of organisms
Genomics is the study of
A) genetic patterns of genome of species
B) genes
C) genes synthesizing proteins
D) genes as diagnostic tools
E)
139
140
141
142
Proteomics is the study of
A) codons
B) proteins
C) proteins and genes
D) genes
Human genome project was completed in
A) January 2003
B) April 2013
C) April 2003
D) January 2013
Proteomics was coined by
A) Robert Koch
B) Marc wilkins
C) Thomas Rederick
D) Elizabeth Bugie
Genomics was coined by
A) Marc wilkins
B) Alexander Fleming
C) Thomas Rederick
D) Albert Schatz
Unit-VIII / Nanotechnology and Nanomedicine
143
144
Who coined the word 'nanotechnology'?
A) Eric Drexler
B) Richard Feynmann
C) Sumio Tijima
D) Richard Smalley
Which formulation contains nanoparticles prepared by using biologically processed
metal ores.
A) Homeopathic medicines
B) Modern antibiotics
C) Ayurvedic 'Bhasmas'
145
146
D) Modern cosmetics
The processing of separation, consolidation and deformation of materials by one
atom or one molecule is called as:
A) Biotechnology.
B) Physics.
C) Nanobiotechnology.
D) Chemistry.
Which is the surface science and physical chemistry that focuses on fabrication of
structures in carbon, silicon or other materials?
A) Wet nanobiotechnology
B) Computational
nanobiotechnology
C) Drug nanobiotechnology
D) Nan biotechnology
147
148
149
150
Which metal is used with nanoparticles for antibiotic delivery?
A) Gold
B) Titanium
C) Zinc
D) Silver
Nano shells are used in the treatment of which of the following disease?
A) Alzheimer’s
B) Cancer
C) HIV
D) Parkinson’s
A Nano biological recognition component, which is involved in interacting with
the analyte molecule is called as:
A) Biosensor
B) Probe
C) Nanobiosensor
D) Quantum Dots
Tiny semiconductor Nanoparticles with fascinating light-emitting properties are
called as:
A) Nanoparticles
B) Nanopores
C) Bucky Balls
D) Quantum Dots