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Transcript
Prod. Code J 4145
NH
H 3C
H 3C
O
O
H 3C
HO
CH3
Cell-permeable steroidal alkaloid
with teratogenic effects; induces
cyclopia by blocking Shh signaling
(IC50 ~500–700 nM in s12 cells).
References
1
Williams, J.A., et al., Identification of a small molecule inhibitor of the
hedgehog signaling pathway: effects on basal cell carcinoma-like lesions.
Proc. Natl. Acad. Sci. USA, 100, 4616-4621 (2003).
2. Mistretta, C.M., et al., Cyclopamine and jervine in embryonic rat tongue
cultures demonstrate a role for Shh signaling in taste papilla development
and patterning: fungiform papillae double in number and form in novel
locations in dorsal lingual epithelium. Dev. Biol., 254, 1-18 (2003).
Prod. Code: S 4944
Clone Name: 171018
Product Form: Purified rat immunoglobulin
Immunogen: Purified, E. coli-derived, recombinant mouse Sonic
hedgehog (Shh) N-terminal peptide (amino acids
25-198)
Isotype: rat IgG2a
Species Cross Reactivity: Human and mouse Shh
Sonic hedgehog (Shh) is an important cell signaling molecule
expressed during embryonic development. Shh is involved in the
patterning of the developing embryonic nervous system, somite
and limb. The N-terminal peptide of Shh is released by autoproteolysis and functions through interactions with a multicomponent receptor complex containing the transmembrane
proteins, Patched and Smoothened. Shh protein is expressed in
key embryonic tissues such as the Hensen’s node, zone of polarizing activity in the posterior limb bud, notochord, and floor
plate of the neural tube.
New Product Highlights
inhibitor; cyclopamine analog
9
Monoclonal Anti-Sonic Hedgehog
(Shh), N-Terminal
Prod. Code H 5163
Transcriptional target in hedgehog signaling; novel regulatory
protein that binds with all three mammalian hedgehogs (Shh,
Dhh, and Ihh). The biological activity is measured by its ability to
inhibit Sonic hedgehog (Shh) induction of alkaline phosphatase
production in C3H10T1/2 fibroblasts.
References
1. Echelard, Y., et al., Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity. Cell, 75,
1417-1430 (1993).
2. Weed, M., et al., The role of sonic hedgehog in vertebrate development.
Matrix Biol., 16, 53-58 (1997).
3. Ingham, P.W. and McMahon, A.P., et al., Hedgehog signaling in animal
development: paradigms and principles. Genes Dev., 15, 3059-3087 (2001).
References
1. Chuang, P.T. and McMahon, A.P., Vertebrate hedgehog signaling modulated by induction of a hedgehog-binding protein. Nature, 397, 617-621 (1999).
2. Ingham, P.W. and McMahon, A.P., et al., Hedgehog signaling in animal
development: paradigms and principles. Genes Dev., 15, 3059-3087 (2001).
Sodium stibogluconate: Irreversible protein
tyrosine phosphatase (PTP) inhibitor
Related Products
Product Name
Cyclopamine
Description
Hedgehog signaling pathway inhibitor
Sonic Hedgehog Peptide, mouse, recombinant
Prod. Code
C 4116
Prod. Code S 5319
S 0191
Inhibits in vitro PTPases Src homology PTPase1 (SHP-1; 10 µg/ml), SHPO
O
2 (100 µg/ml), and PTP1B (100
O
O
OOC
Sb
Sb
O
O - O
µg/ml), but not mitogen-activated
O
OH
H
H
OH
OH
protein kinase phosphatase-1
3Na+
(MKP1), a dual-specificity phosphatase. Inhibition of activity was determined by measuring
dephosphorylation of a synthetic phosphotyrosine peptide by the
GST/phosphatase fusion protein [1]. Routinely used for the treatment of Leishmaniasis in humans, a parasitic disease spread by
the bite of sand flies infected with the protozoa Leishmania
donovani [2].
HO
TOFA: Potent, reversible, cell-permeable and
competitive acetyl-CoA carboxylase (ACC) inhibitor
Prod. Code T 6575
Acetyl-CoA carboxylase (ACC) is a key
enzyme involved in fatty acid biosynthesis. TOFA has been shown to
dose-dependently inhibit fatty acid synthesis in human breast
cancer cell line MCF7 displaying an IC50 value of 4 µM.
HOOC
O
O(CH2)13CH3
References
1. Landree, L.E., et al., C75, a fatty acid synthase inhibitor, modulates AMPactivated protein kinase to alter neuronal energy metabolism. J. Biol.
Chem., 279, 3817-3827 (2004).
2. Thupari, J.N., et al., Fatty acid synthase inhibition in human breast cancer
cells leads to malonyl-CoA-induced inhibition of fatty acid oxidation and
cytotoxicity. Biochem. Biophys. Res. Commun., 285, 217-223 (2001).
HO
COO-
References
1. Pathak, M.K. and Yi, T., Sodium stibogluconate is a potent inhibitor of
protein tyrosine phosphatases and augments cytokine responses in hemopoietic cell lines. J. Immunol., 167, 3391-3397 (2001).
2. Wyllie, S., et al., Dual action of antimonial drugs on thiol redox metabolism in the human pathogen Leishmania donovani. J. Biol. Chem., 279,
39925-39932 (2004).
Related Products
Product Name
Dephostatin
Descriptor
Prod. Code
CD45-associated tyrosine
phosphatase inhibitor
D 8065
NCS 95397
Cdc2 phosphatase inhibitor
N 1786
Sodium orthovanadate
Tyrosine phosphatase inhibitor
S 6508
®
Hedgehog-interacting Protein (Hip), mouse,
recombinant, expressed in NSO cells
Celltransmissions Vol 21, No. 1, 2005 Order: 1-800-325-3010 Technical Service: 1-800-325-5832 sigma-aldrich.com/cellsignaling
Applications: Immunoblotting, immunohistochemistry,
neutralization, and ELISA (capture)
®
Jervine: Sonic hedgehog (Shh) signaling pathway