Download EB nucleic acids AP

AP Biology
Nucleic Acids
 Function:

store & transmit hereditary information
 Examples:
RNA (ribonucleic acid)
 DNA (deoxyribonucleic acid)

 Structure:

AP Biology
monomers = nucleotides
Nucleotides
 3 parts
nitrogen base (C-N ring)
 pentose sugar (5C)

 ribose in RNA
 deoxyribose in DNA

AP Biology
PO4 group
2005-2006
Types of nucleotides
 2 types of nucleotides
different Nitrogen bases
 purines

 double ring N base
 adenine (A)
 guanine (G)

pyrimidines
 single ring N base
 cytosine (C)
 thymine (T)
AP Biology
 uracil (U)
2005-2006
Building the polymer
AP Biology
2005-2006
Nucleic polymer
 Backbone
sugar to PO4 bond
 phosphodiester bond

 new base added to sugar of
previous base
 polymer grows in one direction

N bases hang off the
sugar-phosphate backbone
Why is this important?
AP Biology
2005-2006
RNA & DNA
 RNA

single nucleotide chain
 DNA

double nucleotide chain
 N bases bond in pairs
across chains

spiraled in a double helix
 double helix 1st proposed as structure of DNA
in 1953 by James Watson & Francis Crick
(just celebrated 60th anniversary)
AP Biology
Pairing of nucleotides
 Nucleotides bond between
DNA strands
H bonds
 purine :: pyrimidine
 A :: T

 2 H bonds

G :: C
 3 H bonds
Why is this important?
AP Biology
2005-2006
Information polymer
 Function

series of bases encodes information
 like the letters of a book

stored information is passed
from parent to offspring
 need to copy accurately

stored information = genes
 genetic information
AP Biology
2005-2006
AP Biology
2005-2006
DNA molecule
 Double helix

H bonds between bases
join the 2 strands
 A :: T
 C :: G
Why is it important
that the strands are
bonded by H bonds?
AP Biology
Copying DNA
 Replication

2 strands of DNA helix are
complementary
 have one, can build other
 have one, can rebuild the
whole
why is this a good
system?
 when in the life of a cell
does replication occur?

 mitosis
AP Biology
 meiosis
DNA replication
“It has not escaped our notice that
the specific pairing we have
postulated immediately suggests a
possible copying mechanism for the
genetic material.”
James Watson
Francis Crick
1953
AP Biology
2005-2006
1953 | 1962
Watson and Crick … and others…
AP Biology
2005-2006
Maurice Wilkins… and…
AP Biology
1953 | 1962
2005-2006
Rosalind Franklin (1920-1958)
AP Biology
Interesting note…
 Ratio of A-T::G-C
affects stability
of DNA molecule


2 H bonds vs. 3 H bonds
biotech procedures
 more G-C =
need higher T° to
separate strands

high T° organisms
 many G-C

parasites
 many A-T (don’t know why)
AP Biology
Macromolecule
Review
AP Biology
Carbohydrates
 Structure / monomer

monosaccharide
 Function
energy
 raw materials
 energy storage
 structural compounds

glycosidic bond
 Examples

AP Biology
glucose, starch, cellulose, glycogen
Lipids
 Structure / building block

glycerol, fatty acid, cholesterol, H-C chains
 Function
energy storage
 membranes
 hormones

 Examples

AP Biology
ester bond (in a fat)
fat, phospholipids, steroids
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Proteins
 Structure / monomer
amino acids
 levels of structure

 Function
enzymes
 transport
 signals

defense
◆ structure
◆ receptors
◆
peptide bond
 Examples

AP Biology
digestive enzymes, membrane
channels, insulin hormone, actin
Know the Differences Between
Levels of Protein Structure!
Denaturation of Proteins
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Changing Tertiary Structure…
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Denaturation and the Active Site
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Nucleic acids
 Structure / monomer

nucleotide
 Function

information storage
& transfer
 Examples

AP Biology
DNA, RNA
phosphodiester bond
Why does ethanol precipitate DNA?
 DNA is negatively charged because of




phosphate backbone
Ion-dipole interactions between water
and phosphate stabilize DNA structure
Lots of ethanol removes water of
hydration around phosphates
Na+ ions (from salt) step in and force
changes in DNA structure, leading to
precipitation
Wiki article on ethanol precipitation
very helpful
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Denatured (precipitated) DNA
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Grid In Question on Nucleic Acids
 A scientist extracts DNA from the
nucleus of cells and sequences it. The
scientist determines that 27% of the
nucleotide bases are guanine. What
percentage of the bases are thymine?
 In DNA, G=C and A=T
 If 27% are G, then 27% are C: 54% G=C
 Therefore 46% of bases are A=T;
 23% thymine and 23% adenine
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Practice Grid-in 2
 A sample of DNA contains 21%
adenine. What percentage of the DNA is
cytosine?
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