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Download pNZ:vig Vector information: IRES
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Transcript
pNZ:vig Vector information: IRES-incorporated lactococcal bicistronic vector Location of features Cytomegalovirus promoter: 14-669 VP2 gene from UPM 04190:6982110* EMCV IRES: 2086- 2660 Zoanthus sp. GFP: 2661- 3353 Bovine growth hormone polyA signal: 3381-3614 repC: 4339-4548 repA: 4816-5514 chloramphenicol resistance gene: 5973-6623 Propagation in Lactococcus lactis Suitable host strain: NZ9000 and other NICE system vectors derivatives Selection marker: plasmids confer resistance towards chloramphenicol ( 7.5 µg/ml) in L. lactis Description of the Invention The foreign DNA inserts are introduced into lactococcal vector in this manner; eukaryotic promoter, followed by the VP2 gene (coding for VP2 protein from infectious bursal disease virus, the first gene to be transcribed by the promoter), IRES, gfp gene (gene encoding for green fluorescent protein, the second gene to be transcribed by IRES) and transcription terminator signal polyA . The IRES element must be placed between 2 cistronic regions. The transcription initiated by eukaryotic promoter will promptly transcribe the VP2 gene, IRES element and the gfp gene. The translation initiation will both occur in the cap region of the VP2 gene and IRES structure. Both genes can replace with any gene of interest. 1
