Download pNZ:vig Vector information: IRES

pNZ:vig Vector information: IRES-incorporated lactococcal bicistronic vector
Location of features

Cytomegalovirus promoter: 14-669

VP2 gene from UPM 04190:6982110*

EMCV IRES: 2086- 2660

Zoanthus sp. GFP: 2661- 3353

Bovine growth hormone polyA
signal: 3381-3614

repC: 4339-4548

repA: 4816-5514

chloramphenicol resistance gene:
5973-6623
Propagation in Lactococcus lactis

Suitable host strain: NZ9000 and other NICE system vectors derivatives

Selection marker: plasmids confer resistance towards chloramphenicol ( 7.5 µg/ml) in
L. lactis
Description of the Invention
The foreign DNA inserts are introduced into lactococcal vector in this manner; eukaryotic
promoter, followed by the VP2 gene (coding for VP2 protein from infectious bursal disease
virus, the first gene to be transcribed by the promoter), IRES, gfp gene (gene encoding for
green fluorescent protein, the second gene to be transcribed by IRES) and transcription
terminator signal polyA . The IRES element must be placed between 2 cistronic regions. The
transcription initiated by eukaryotic promoter will promptly transcribe the VP2 gene, IRES
element and the gfp gene. The translation initiation will both occur in the cap region of the
VP2 gene and IRES structure. Both genes can replace with any gene of interest.
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