Download 10 - LifeSciTRC

Application Exercise
Date: November 14, 2014
Time: 8 a.m. to 9 a.m.
Presenter: Brian N. Griffith, Ph.D.
What is the energy cost to switching from aerobic to
anaerobic metabolism
A.
C.
100 % reduction
95% reduction in
energy
25%
10
A.
25%
B.
B.
50 % reduction
D.
5% reduction
25%
C.
25%
D.
Points
15. 1,500
14. 1,400
13. 1,300
12. 1,200
11. 1,100
10. 1,000
9. 900
8. 800
7. 700
6. 600
5. 500
4. 400
3. 300
2. 200
1. 100
Normoxic
Ischemia
Energy
Sources
Reperfusion
Baseline
Glucose
ATP
Fatty
acids
NAD+
ATP
Cell Homeostasis
NAD+
ATP
NAD+
Allostasis
What metabolite(s) is produced in cardiomyocytes
undergoing anaerobic metabolism
A. Citrate
B. Acetyl-CoA
C. Lactate
D. Ethanol
0%
10
A.
0%
B.
0%
C.
0%
D.
Points
15. 1,500
14. 1,400
13. 1,300
12. 1,200
11. 1,100
10. 1,000
9. 900
8. 800
7. 700
6. 600
5. 500
4. 400
3. 300
2. 200
1. 100
Cardiac failure cause by an MI is attributed to:
B. MPTP opening
A. Necrosis and Apoptosis
D. Increase in
intracellular calcium
C. All of them
0%
10
A.
0%
B.
0%
C.
0%
D.
Points
15. 1,500
14. 1,400
13. 1,300
12. 1,200
11. 1,100
10. 1,000
9. 900
8. 800
7. 700
6. 600
5. 500
4. 400
3. 300
2. 200
1. 100
Reperfusion Injury
1. Myocardial Infarct:
2. Loss of Aerobic Respiration (Ox-Phos)
3. Increase in Lactic Acid Production
4. Decrease in ATP
5. Decrease in Adenine Nucleotide (Purine Degradation Pathway)
6. Increase in intracellular Ca+2
7. The Overall Effect is Cell Damage and Eventually cell Death.
Which protein complex is directly reduced by
dihydroquinol?
B. Complex III
A. Complex I
C. Complex II
0%
10
A.
D. Complex IV
0%
B.
0%
C.
0%
D.
Points
15. 1,500
14. 1,400
13. 1,300
12. 1,200
11. 1,100
10. 1,000
9. 900
8. 800
7. 700
6. 600
5. 500
4. 400
3. 300
2. 200
1. 100
What is the primary effect of carbon monoxide
consumption?
A. It inhibits complex IV
B. It increases NADH and
FADH2 synthesis
C. It decreases
complex I activity
D. It inhibits ATP synthesis
0%
10
A.
0%
B.
0%
C.
0%
D.
Points
15. 1,500
14. 1,400
13. 1,300
12. 1,200
11. 1,100
10. 1,000
9. 900
8. 800
7. 700
6. 600
5. 500
4. 400
3. 300
2. 200
1. 100
Participant Scores
Points
Participant
Points
Participant
Ischemia-Reperfusion injury can cause which of the
following?
A.Lactic acid production
B.ATP production
C. A decrease in pH
0%
10
A.
D. ROS production
0%
B.
0%
C.
0%
D.
Points
15. 1,500
14. 1,400
13. 1,300
12. 1,200
11. 1,100
10. 1,000
9. 900
8. 800
7. 700
6. 600
5. 500
4. 400
3. 300
2. 200
1. 100
Reperfusion Injury
1. Increase in ROS (Reactive Oxygen Species)
2. NADPH, Xanthine Oxidase, Complex I & III
3. Inhibition of Ox-Phos allows ubiquinone to form ubisemiubiquinone.
4. Opening of the mitochondrial permeability transition pore (MPTP).
Opening of MPTP can serve as an uncoupler to Ox-Phos
5. Opening of MPTP allows cytochrome C to leak out of the
mitochondria (APOPTOSIS)
6. The Overall Effect is Cell Damage and Eventually cell Death.
Which of the following enzymes is directly
responsible for removing the free radical
superoxide?
A. SOD
B. Catalase
C. Vitamin E
D. Gluthione
0%
10
A.
0%
B.
0%
C.
0%
D.
Points
15. 1,500
14. 1,400
13. 1,300
12. 1,200
11. 1,100
10. 1,000
9. 900
8. 800
7. 700
6. 600
5. 500
4. 400
3. 300
2. 200
1. 100
A 25-year-old patient with chronic fatigue caused by
iron deficiency anemia can best be explained by
which of the following mechanisms?
A. A decrease in oxphos
B. A decrease in CoQ
D. A mutation in
Hemoglobin
C. A mutation in SOD
0%
10
A.
0%
B.
0%
C.
0%
D.
Points
15. 1,500
14. 1,400
13. 1,300
12. 1,200
11. 1,100
10. 1,000
9. 900
8. 800
7. 700
6. 600
5. 500
4. 400
3. 300
2. 200
1. 100
A 52-year-old stock broker commits suicide using
cyanide (CN-). Which of the following mechanisms is
NOT caused by cyanide poising?
A. OX-Phos will be
inhibited
B. Some complexes will
be oxidized
C. NADH will be oxidized
D. ATP production will
be decreased
0%
10
A.
0%
B.
0%
C.
0%
D.
Points
15. 1,500
14. 1,400
13. 1,300
12. 1,200
11. 1,100
10. 1,000
9. 900
8. 800
7. 700
6. 600
5. 500
4. 400
3. 300
2. 200
1. 100
A newly discovered inhibitor was found to cause the
accumulation of NADH+, reduced CoQ, but not reduced
cytochrome c. The inhibitior most likely:
A. Complex I
B. Complex IV
C. Complex II
D. Complex III
0%
10
A.
0%
B.
0%
C.
0%
D.
Points
15. 1,500
14. 1,400
13. 1,300
12. 1,200
11. 1,100
10. 1,000
9. 900
8. 800
7. 700
6. 600
5. 500
4. 400
3. 300
2. 200
1. 100
Participant Scores
Points
Participant
Points
Participant
What are the biochemical uses of ATP in a
myocardial cell?
A. ROS production
B. Na/K gradients
D. Fatty acid synthesis
C. Oxidation of LDLs
0%
10
A.
0%
B.
0%
C.
0%
D.
Points
15. 1,500
14. 1,400
13. 1,300
12. 1,200
11. 1,100
10. 1,000
9. 900
8. 800
7. 700
6. 600
5. 500
4. 400
3. 300
2. 200
1. 100
What is ischemia?
A. Hypoxia causes ischemia
C. Lack of blood flow
0%
10
A.
B. A form of cell death
D. Lack of Carbon dioxide
0%
B.
0%
C.
0%
D.
Points
15. 1,500
14. 1,400
13. 1,300
12. 1,200
11. 1,100
10. 1,000
9. 900
8. 800
7. 700
6. 600
5. 500
4. 400
3. 300
2. 200
1. 100
Which of the following are thought to protect heart
cells against irreversible ischemia-induced injury?
A. Anaerobic Metabolism
B. Closing of MPTP pore
C. Decrease in pH
D. All of the above
0%
10
A.
0%
B.
0%
C.
0%
D.
Points
15. 1,500
14. 1,400
13. 1,300
12. 1,200
11. 1,100
10. 1,000
9. 900
8. 800
7. 700
6. 600
5. 500
4. 400
3. 300
2. 200
1. 100
One of the following statements is NOT true. During
a heart attack and when the cell is oxygen starved,
anaerobic glycolysis will
A. Be activated by low pO2
B. Produce ATP by Ox-Phos
C. Produce lactate
0%
10
A.
D. Result in lower pH
0%
B.
0%
C.
0%
D.
Points
15. 1,500
14. 1,400
13. 1,300
12. 1,200
11. 1,100
10. 1,000
9. 900
8. 800
7. 700
6. 600
5. 500
4. 400
3. 300
2. 200
1. 100
Which of the following enzymes is responsible for
the conversion of H2O2 to H2O.
A. Oxygen reductase
B. SOD
C. Cytochrome C
D. Catalase
0%
10
A.
0%
B.
0%
C.
0%
D.
Points
15. 1,500
14. 1,400
13. 1,300
12. 1,200
11. 1,100
10. 1,000
9. 900
8. 800
7. 700
6. 600
5. 500
4. 400
3. 300
2. 200
1. 100
Participant Scores
Points
Participant
Points
Participant
And the winner is: