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B. Insect/machine combinations C. Synthetic Transplants D. Animal Surrogate Transplants E. Engineered Plants/Animals Types of Biotechnology Applications 1. Blue Biotechnology - Marine and aquatic applications - rare 2. Green Biotechnology -agricultural applications -micropropagation -transgenic plants (Roundup ready beans, Bt Corn) 3. Red Biotechnology - Medical applications Ex. Antibiotics, genetic manipulations 4. White Biotechnology - Industrial applications Ex. Catalysts and enzymes Review of the Cell and Chemistry Basic Organic Chemistry Monomer: a subunit of a larger macromolecue Polymer: a large macromolecule made of monomers 4 Most Important Biological Polymers 1. Lipids: greasy compounds that do not dissolve in water Monomer: fatty acids Important Lipids: 1. Steroids 2. Phospholipids: cell membrane 2. Carbohydrates: twice the hydrogen atoms as oxygen Monomer: monosaccharides: simple sugars (C6H12O6) Disaccharides: 12 carbon, double sugars Polysaccharides: chain of monosaccharides (cellulose; starch) 3. Proteins: polymer of many amino acids 4. Nucleic Acids: - Monomers: - Large macromolecules found in the nucleus of cells - Contain genetic information - Deoxyribonucleic acid (DNA) - Ribonucleic Acid (RNA) - Four bases: Adenine (A), thymine (T), cytosine (C), Guanine (G) Cell Theory – 3 postulates 1. All cells come from pre-existing cells 2. Cells are the basic unit of life 3. All living organisms are composed of cells Two Types of Cells 1. Prokaryotic Cells: small simple cells -lack a nucleus -DNA called chromatin 2. Eukaryotic Cells: complex cells -membrane bound nucleus -DNA in nucleus -complex organelles Cell Organelles: Cell’s “organs” 1. Cell Membrane: FX: regulates what enters/leaves the cell -phospholipid bi-layer - 2. Nucleus: FX: 1. store DNA 2. control center of the cell 3. Cytoplasm: gel like substance in the cell FX: site of all cell activities 4. Endoplasmic Reticulum Two Types 1. Rough ER: makes and transport proteins (has ribosomes) 2. Smooth ER: transport proteins, makes steroids 5. Ribosomes: small organelles FX: produce proteins -500,000 per cell 6. Mitochondria: rod shaped; double membrane FX: Produce energy molecules – adenosine triphosphate (ATP) “Powerhouse” of the cell 7. Golgi Apparatus: flat sacs FX: Package, sort, and transport proteins 8. Lysosome: FX: to break down foreign contaminants Filled with enzymes 9. Microtubules FX: support the cell shape Cytoskeleton 10. Centrioles: Cylindrical structures FX: assist in cell division Other Organelles -Chloroplast: used for capturing sunlight for photosynthesis -Cilia: small hair like structures used for movement -Flagella: “tail” used for locomotion Cellular Respiration -set of metabolic reactions -Produces ATP (adenosine triphosphate) -breaks down carbs Major Components: A. Glycolysis B. Krebs Cycle C. Electron Transport D. Oxidative Phosphorylation Glycolysis -occurs in cytoplasm -aerobic or anaerobic Net Reaction: Glucose 2 pyruvate2 ATP Krebs Cycle - Takes pyruvate - Creates electron carriers - Produces NADH, FADH2, NADPH - Occurs in mitochondria Electron Transport Chain - Moves electrons through mitochondrial membrane - Uses protienaceous electron carriers - Used to move protons out of mitochonria - Produces chemical gradient - Moves ATP synthetase Oxidative Phosphorylation - Requires Adenosine Diphosphate (ADP) and inorganic phosphate - Protons cause ATP Synthase to bond ADP and Pi together