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The Characterization of Myeloid Cell Subsets in Innate and Adaptive Immune Response against Listeria monocytogenes Infection in skin draining Lymph node Jiyoun Min, Suk-jo Kang* Department of Biological Sciences, KAIST, South Korea Innate and adaptive immunity are well designed defense system against to infection of pathogen. Dendritic cells (DCs) play major role in activation of immune response by capturing, processing and presenting antigen to naïve T cell in lymphoid organs. DCs are important player in the protective immunity to Listeria monocytogenes, intracellular bacteria. Although studies on the roles of DC in immunity are in progress, definite classification of DC subsets is still unclear. Furthermore, innate immune responses are not defined well compared to adaptive immune response against Listeria. In particular, immunity in secondary lymphoid organ such as lymph node (LN), there are much more complicated network among immune cells. Therefore I focused on the induction of immune responses in draining-LN against Listeria infection through skin. Myeloid cell subsets can be distinguished with their surface markers. Dendritic cell of skindraining lymph node is subdivided into two groups based on their location; resident DC which reside in LN and migratory DC which migrate from skin to LN. Although previous studies showed that migratory DC involves five subtypes of DC, but these classification is not clear yet. Therefore I further distinguished them by using knock out and bone marrow chimeric mice. After Listeria infection through skin, various immune cells as well DCs infiltrated to draining LN, some of these cells formed clusters and produced cytokine. We characterized kinetics of these responses by flow cytometry and immunochemistry experiments to define their roles in discriminative immune responses against Listeria infection.