Download The Characterization of Myeloid Cell Subsets in Innate and Adaptive

The Characterization of Myeloid Cell Subsets in Innate and Adaptive
Immune Response against Listeria monocytogenes Infection in skin
draining Lymph node
Jiyoun Min, Suk-jo Kang*
Department of Biological Sciences, KAIST, South Korea
Innate and adaptive immunity are well designed defense system against to infection of
pathogen. Dendritic cells (DCs) play major role in activation of immune response by
capturing, processing and presenting antigen to naïve T cell in lymphoid organs. DCs are
important player in the protective immunity to Listeria monocytogenes, intracellular bacteria.
Although studies on the roles of DC in immunity are in progress, definite classification of DC
subsets is still unclear. Furthermore, innate immune responses are not defined well compared
to adaptive immune response against Listeria. In particular, immunity in secondary lymphoid
organ such as lymph node (LN), there are much more complicated network among immune
cells. Therefore I focused on the induction of immune responses in draining-LN against
Listeria infection through skin.
Myeloid cell subsets can be distinguished with their surface markers. Dendritic cell of skindraining lymph node is subdivided into two groups based on their location; resident DC
which reside in LN and migratory DC which migrate from skin to LN. Although previous
studies showed that migratory DC involves five subtypes of DC, but these classification is
not clear yet. Therefore I further distinguished them by using knock out and bone marrow
chimeric mice.
After Listeria infection through skin, various immune cells as well DCs infiltrated to
draining LN, some of these cells formed clusters and produced cytokine. We characterized
kinetics of these responses by flow cytometry and immunochemistry experiments to define
their roles in discriminative immune responses against Listeria infection.