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Biology 231 Anatomy and Physiology I Sylvania Laboratory Survival Guide Table of Contents Lab Topic (Exercise in Marieb Manual): page: (in lab packet) Safety Guidelines 2 Disposal Guidelines 3 The Language of Anatomy (Exercise 1, page 1) Organ Systems Overview (Exercise 2, page 10) The Microscope (Exercise 3, page 21) The Cell Anatomy and Division (Exercise 4, page 30 and 5A, page 40) Histology Study Guide (Exercise 6A, page 48) Integumentary System and Membranes (Exercises 7 and 8, beginning on page 67) Bone Histology, Axial & Appendicular Skeleton (Exercises 9, 10, 11, 12, 13 beginning on page 81) Muscles Study Guide (Exercise 14, 15, beginning on page 132) and Nerve Histology (Exercise 17, beginning on page 185) 5 p.1 Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide 7 8 9 12 17 18 24 33 BI 231 Human Anatomy and Physiology Survival Guide Updated August, 2005 1. Upon entering the laboratory, please locate the exits, fire extinguisher, eyewash station, and clean up materials for chemical spills. The location of fire blanket, safety kit, and showers will be demonstrated by your instructor. 2. Read the general laboratory directions and any objectives before coming to lab. 3. Food and drink, including water, is prohibited in laboratory. This is per Federal laboratory guidelines and per College Safety Policy. Do not chew gum, use tobacco products of any kind, store food or apply cosmetics in the laboratory. No drink containers of any kind may be on the benches. 4. Please keep all personal materials off the working area. Store backpacks and purses at the rear of the laboratory, not beside or under benches. Some laboratory spaces have shelving in rear for this purpose. 5. For your safety, please restrain long hair, loose fitting clothing and dangling jewelry. Hair ties are available, ask your instructor. Hats and bare midriffs are not acceptable in the laboratory. Shoes, not sandals, must be worn at all times in laboratory. You may wear a laboratory apron or lab coat if you desire, but it is not required. 6. We do not wish to invade your privacy, but for your safety if you are pregnant, taking immunosuppressive drugs or who have any other medical conditions (e.g. diabetes, immunological defect) that might necessitate special precautions in the laboratory must inform the instructor immediately. If you know you have an allergy to latex or chemicals, please inform instructor. 7. Decontaminate work surfaces at the beginning of every lab period using Amphyl solution. Decontaminate bench following any practical quiz, when given, and after labs involving the dissection of preserved material. 8. Use safety goggles in all experiments in which solutions or chemicals are heated or when instructed to do so. Never leave heat sources unattended: hot plates or Bunsen burners. 9. Wear disposable gloves when handling blood and other body fluids or when touching items or surfaces soiled with blood or other body fluids such as saliva and urine. (NOTE: cover open cuts or scrapes with a sterile bandage before donning gloves.) Wash hands immediately after removing gloves. 10. Keep all liquids away from the edge of the lab bench to avoid spills. Immediately notify your instructor of any spills. Keep test tubes in racks provided, except when necessary to transfer to water baths or hot plate. You will be advised of the proper clean-up procedures for any spill. 11. Report all chemical or liquid spills and all accidents, such as cuts or burns, no matter how minor, to the instructor immediately. 12. Use mechanical pipetting devices only. Mouth pipetting is prohibited. Students who do not comply with these safety guidelines will be excluded from the Laboratory p.2 Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide Disposal of contaminated materials: 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. Place disposable materials such as gloves, mouth pieces, swabs, toothpicks and paper toweling that have come into contact with blood or other body fluids into a disposable Autoclave bag for decontamination by autoclaving. This bucket is not for general trash. Place glassware contaminated with blood and other body fluids directly into a labeled bucket of 10% Bleach solution. ONLY glass or plastic-ware is to be placed in this bucket, no trash. Sharp’s container is for used lancets only. It is bright red. When using disposable lancets do not replace their covers. Properly label glassware and slides, using china markers provided. Wear disposable gloves when handling blood and other body fluids or when touching items or surfaces soiled with blood or other body fluids such as saliva and urine. (NOTE: cover open cuts or scrapes with a sterile bandage before donning gloves.) Wash hands immediately after removing gloves. Wear disposable gloves when handling or dissecting specimens fixed with formaldehyde or stored in Carosafe/Wardsafe. Wear disposable gloves when handling chemicals denoted as hazardous or carcinogenic by your instructor. Read labels on dropper bottles provided for an experiment, they will indicate the need for gloves or goggles, etc. Upon request, detailed written information is available on every chemical used (MSDS). Ask your instructor. No pen or pencil is to be used at any time on any model or bone. The bones are fragile, hard to replace and used by hundreds of students every year. To protect them and keep them in the best condition, please use pipe cleaners and probes provided instead of a writing instrument. Probes may be used on models as well. The bones are very difficult and costly to replace, as are the models and may take a long time to replace. At the end of an experiment: clean glassware and place where designated. Remove china marker labels at this time. return solutions & chemicals to designated area. Do not put solutions or chemicals in cupboards! You cannot work alone or unsupervised in the laboratory. Microscopes should be cleaned before returning to numbered cabinet. Be sure objectives are clean, use lens paper. Place objectives into storage position, and return to the storage cabinet. Be sure cord has been coiled and restrained. Your instructor may require microscope be checked before you put it away. Be sure it is in assigned cupboard. Please replace your prepared slides into the box from which they came (slides and boxes are numbered), so students using them after you will be able to find the same slide. Clean slide with Kimwipes if dirty or covered with oil, before placing back in box. If you break a slide, please, inform you instructor so the slide can be replaced. Please be aware that there are hundreds of dollars worth of slides in each box and handle the boxes with care when carrying to and from your work bench. Be sure all paper towels used in cleaning lab benches and washing hands are disposed of in trash container provided. Students who do not comply with these safety guidelines and directions will be excluded from the Laboratory p.3 Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide When specimens in laboratory such as human bones (or other body parts), they may seem like inanimate objects that are far removed from a living organism. We ask that you remain alert to the fact specimens were once parts of humans (or other living creatures). Please handle these body parts with respect and care. p.4 Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide Language of Anatomy (Exercise 1) 1. Define gross anatomy; differentiate it from Histology. ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ 2. Describe the anatomical position. ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ 3. Be able to describe, define, and locate the following regions: Abdominal Antebrachial Antecubital Axillary Brachial Buccal Carpal Cervical Deltoid Digital Femoral Frontal Inguinal Mammary Mental Nasal Oral Orbital Palmar Patellar Pedal Pelvic Pubic Sternal Tarsal Thoracic Umbilical Acromial Calcaneal Cephalic Dorsum Gluteal Lumbar Occipital Olecranal Otic Perineal Plantar Popliteal Sacral Scapular Vertebral 4. Define the following terms: Superior Inferior Anterior Posterior Medial Lateral Cephalic / Cephalad Caudal Dorsal Ventral Superficial Deep Proximal Distal p.5 Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide Note: these terms are also used for hollow organs: the upper esophagus is proximal, the lower esophagus is more distal. 5. Describe the following anatomical planes; give alternate terms if possible. Frontal Midsagittal Parasagittal Transverse Which plane could never cut through the kidneys? What is a longitudinal incision through the small intestine? 6. 7. Locate the following body cavities. Use your Tortora textbook (lecture text) for this: it provides better illustrations and gives more detail! a. Dorsal b. Ventral: subdivided into thoracic and abdominopelvic. The thoracic cavity contains smaller cavities inside: Name three. Here are your hints: a. Which cavity is in between the lungs and contains the heart, trachea, and esophagus? b. Which cavity surrounds the heart, and is located in between the parietal and visceral pericardium? c. Which cavity surrounds the lungs, and is located in between the parietal and visceral pleura? d. Which cavity is in the abdominal cavity, and is in between the parietal and visceral peritoneum? e. Is the the liver actually in this cavity, or is it better to say that the liver is surrounded by this cavity? (Think of a down coat. When you put the coat on, are you in the coat? Are you in the cavity containing the down?) 8. Define pericarditis_____________________________________________________ 9. Locate the right upper quadrant, right lower quadrant, left upper quadrant, left lower quadrant. 10. Locate the 9 abdominal regions. Pay the most attention to the midline ones: a. epigastric region b. umbilical region c. hypogastric (=suprapubic) region Often these midline regions are used with the quadrants. Example: The pain of appendicitis begins in the periumbilical region (peri=around) and shifts to the right lower quadrant. Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.6 Organ Systems Overview (Exercise 2) 1. 2. 3. 4. Name the human organ systems, and learn the chief functions of each organ system. List two or three organs of each system. Identify these organs on models, rats, and/or cadaver if available. Be able to identify the following organs and structures: a. Thymus b. Heart c. Lungs d. Parietal Pleura e. Visceral Pleura f. Trachea g. Bronchi h. Esophagus i. Diaphragm j. Stomach k. Small Intestine l. Mesentery m. Greater Omentum n. Large Intestine: Identify Cecum (first part of large intestine) o. Pancreas p. Spleen q. Liver r. Kidneys s. Adrenals t. Ureter u. Urinary Bladder v. Male: Scrotum, testes, vas deferens, seminal vesicles, prostate, penis w. Female: Ovaries, fallopian tubes, uterine horns in rat (don't call these fallopian tubes), uterus in human notes: Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.7 The Microscope (Exercise 3) 1. Learn the care of the miscroscope, as described in your lab manual. Which objective should never be used with oil? 2. Learn the parts of the microscope, such as a. ocular lenses, objectives lenses b. nosepiece, arm, stage c. substage light, iris diaphragm lever, condenser d. coarse adjustment knob, fine adjustment knob e. power switch, light control 3. Know the formula for calculating magnification: Total magnification = (magnification of ocular) x (magnification of objective). Example: the total magnification is 450x when using a 10x ocular is 10x and 45x objective. 4. Learn how to focus the microscope using the 10x, 45x (or 40x depending on your scope), and 100x objective lens. 5. Learn how to perform a wet mount of cheek scrapings. (Follow instructor’s directions.) Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.8 Exercise 4 The Cell: Anatomy and Division Be able to identify (on model) and know basic functions of the following cellular structures: nucleus chromatin: what is the difference between this and chromosomes? nucleolus plasma membrane ribosomes smooth endoplasmic reticulum rough endoplasmic reticulum Golgi apparatus Lysosomes Peroxisomes Mitochondria Centrioles What's the difference between mitosis and cytokinesis? 3. Be able to identify (from both microscopic slides of onion root and whitefish blastula) the different phases of the cell cycle: a. interphase b. prophase c. metaphase d. anaphase e. telophase 4. In anaphase, are the structures that are separating called chromatids or daughter chromosomes? 5. Which phase of the cell cycle has chromatin? 6. What is the name for the arrangement of microtubules that helps separate chromosomes? Hint: it is attached to the centrioles. 7. Which phase of mitosis involves chromosomes lined up along the equatorial plane? 8. Cytokinesis begins during late_____________ and extends through (and slightly beyond) _________________ Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.9 The Cell (Exercise 5A) Transport Mechanisms and Cell Permeability 1. Define the two passive cell processes: a. Diffusion b. Filtration 2. What is the driving force of diffusion? 3. What determines the direction of diffusion? 4. What is the difference between simple diffusion and osmosis? 5. Diffusion of dye through agar gel: If methylene blue has a molecular weight of 320 and potassium permanganate has a weight of 158, which should diffuse faster through agar? _________________________________ 6. Observing diffusion through nonliving membranes. Our three artificial cells will be constructed from sealed tubes. The tubing is a semipermeable membrane (like a cell membrane). The “intracellular fluid,” inside the sac and the “extracellular fluid” in the beaker is different in each of our three artificial cells. Sac 1: 40% glucose in the sac, in a beaker of distilled water weight of sac before hour long soak: _________________________________ weight of sac after hour long soak: _________________________________ net change: _________________________________ Result of Benedict's test of beaker fluid: _________________________________ What color does the blue benedict's reagant change to after boiling it (if a sugar such as glucose is present)? _________________________________ Conclusion regarding diffusion of glucose through sac: _________________________________ Sac 3: 10% NaCl in the sac, in a beaker of distilled water weight of sac before hour long soak: _________________________________ weight of sac after hour long soak: _________________________________ net change: _________________________________ Results of testing beaker fluid with Silver Nitrate (AgNO3): Did white precipitate form?___________ Conclusion regarding diffusion of NaCl through sac: _________________________________ Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.10 Sac 4: 1% starch in the sac, in a beaker of 40% glucose weight of sac before hour long soak:_________________________________ weight of sac after hour long soak: _________________________________ net change: _________________________________ Results of testing beaker fluid with Lugol's solution: _________________________________ (What color does Lugol's solution=IKI change to in presence of starch?)_____________ Did starch diffuse through the sac? _________________________________ What conclusions can you make about the relative sizes of glucose, starch, and NaCl? __________________________________________________________________ Looking at the concentrations of the various solutions, what seems to make water diffuse the fastest? ________________________________________________________________________ ____________________________________________________________ 7. Define the following terms: isotonic_________________________________________________________________ hypertonic_______________________________________________________________ crenation________________________________________________________________ hypotonic________________________________________________________________ hemolysis________________________________________________________________ Note: Isotonic saline has the same salinity as plasma; it is 0.9%NaCl. Would you call 0.45% hypertonic or hypotonic? _________________________________ What about 1.5%NaCl? _________________________________ What are the three types of endocytosis? (Distinguish between the three). ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ _______________________________________ Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.11 Histology Study Guide (Exercise 6) We will limit our focus to epithelial and connective tissue because we will study nervous and muscle tissue later this year. Answer the following questions: 1. Rearrange the following from highest to lowest order of magnitude: atoms, cells, tissues, organs, molecules ________________________________________________________________________ 2. List the distinguishing features of epithelial tissue: ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ _______________________________________________________________________ 3. Describe the way that epithelial tissues are named 1st part of name:_______________________________________________________________ 2nd part of name:______________________________________________________________ 4. Name the two epithelial tissues that do not follow the naming process in #3, and explain the meaning of their names: _______________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ 5. On your own paper, draw a concept map connecting the following terms with their functions: squamous cells, cuboidal cells, columnar cells 6. For each epithelial tissue type, in the left hand column below its name, draw a brief sketch of its appearance and write a short phrase reminding you of what it looks like. For example, underneath a sketch of simple squamous epithelium write “fried eggs”. In the middle column write down the functions the type is especially suited for and why. For example, for simple squamous write: “allows easy diffusion because of thin shape.” In the right hand column list one representative location that helps you remember its function(s). Also, be able to identify these tissues from microscope slides. Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.12 Epithelial Tissue, sketch, and reminder of what it looks like simple squamous Functions this type is especially suited for and why. One location that helps you remember its function simple cuboidal nonciliated simple columnar ciliated simple columnar ciliated pseudostratified columnar non-keratinized stratified squamous keratinized stratified squamous Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.13 transitional 7. List the distinguishing features of connective tissue, especially focusing on how it differs from epithelial tissue: _______________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ 8. Differentiate between matrix, ground substance, and fibers. a. Matrix: _______________________________________________________ b. Ground Substance: ______________________________________________ c. Fibers:________________________________________________________ 9. If you put Libby’s fruit in Jell-O and added celery fibers, what would be included in the: a. connective tissue?________________________________________________ b. matrix? ________________________________________________________ c. fibers? _________________________________________________________ d. ground substance?________________________________________________ 10. For the 3 fiber types in connective tissue, in the first column draw the fiber(s). Sketch the fiber and briefly describe some structure in your life that they remind you of. In the middle column write down the functions the type of fiber is especially suited for and why. In the third column list a location of the fibers that helps you remember them. Be able to identify these from a slide of loose areolar tissue. fiber type, sketch, and reminder Elastic function(s) & why fiber suited for this example of where found reticular collagen Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.14 11. Give an example of fluid, gel, and solid ground substance. a. fluid________________________________ b. gel_________________________________ c. solid________________________________ 12. List the functions of the following cells a. fibroblasts_________________________________________________________ _________________________________________________________________ b. mesenchymal_______________________________________________________ __________________________________________________________________ c. adipocytes_________________________________________________________ __________________________________________________________________ d. macrophages_______________________________________________________ __________________________________________________________________ e. plasma cells __________________________________________________________________ __________________________________________________________________ f. mast cells __________________________________________________________________ __________________________________________________________________ 13. For each connective tissue type, in the left hand column below its name, draw a brief sketch of its appearance; label cells, ground substance, & fibers. In the middle column write down the functions the type of connective tissue is especially suited for and why. For example, for adipose tissue write: “energy storage because of huge fat droplets inside cells; insulation because of the thick fatty layer under the skin.” In the right hand column list one representative location that helps you remember its function(s). Connective Tissue type, sketch cells, matrix Functions this type is especially suited for and why. One location that helps you remember its function loose areolar Adipose Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.15 dense regular dense irregular Elastic hyaline cartilage bone blood If your instructor has time, also look slides of : Muscle tissue: skeletal, smooth, and cardiac Nervous tissue: look at multipolar neuron Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.16 Integumentary System and Membranes (Exercises 7 and 8) 1. Identify from model, microscope slide, pictures: a. Epidermis: subdivided into stratum corneum, stratum granulosum, stratum spinosum, stratum basale. Note: also see a stratum lucidum in palms and soles. b. Dermis: subdivided into papillary and reticular dermis c. Hypodermis=superficial fascia: note adipose tissue d. Hair root e. Hair shaft f. Hair follicle: note the enlarged, deepest region called the hair bulb. Also note the projection of dermis at the base of the bulb: the papilla. g. Eccrine sweat gland h. Sebaceous gland i. Arrector pili muscle j. Pacinian corpuscle (look at fetal palm slide) 2. From a surface view of the fingernail a. free edge b. body c. lateeral fold d. lunula e. cuticle 3. From a sagittal section, note the a. root of the nail b. matrix 4. What is the difference between eccrine sweat glands and apocrine sweat glands? Exercise 8: Body Membranes 1. Define mucous membranes. 2. Note the histology of trachea, esophagus, and small intestine: which is lined with ciliated epithelium? 3. How do serous membranes differ from mucous membranes? 4. Are synovial membranes lined with connective tissue or epithelium? Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.17 Bone Histology, Axial & Appendicular Skeleton (Exercises 9, 10, 11, 12, 13) Part 1: Bone-Histology Models and Slides: 1. Spaces: canaliculi, Haversion Canal, lacunae, Volkman's Canal Which way do vessels in Haversion Canals run as opposed to Volkman's? 2. Cells: Osteocytes, Osteoblasts, Osteoclasts What are the functions of each? 3. Coverings: Periosteum, endosteum 4. Types of bone: compact (also called cortical bone), trabecular 5. Marrow types, where located: red, yellow 6. Special structures: trabeculae Long bone structure: from long bone, identify 1. Diaphysis, epiphyses, metaphyses, epiphyseal plate(disk) Is there an epiphyseal plate in adults? Explain. 2. Medullary cavity, endosteum, periosteum 3. Red vs. yellow marrow Part 2: Axial Skeleton 1. Know its components: skull, spine, thoracic cage, hyoid bone 2. Cranial Bones: recognize from inside & outside a. Frontal b. Parietals-pair c. Temporals-pair d. Occipital e. Sphenoid f. Ethmoid g. What is the difference between cranial bones and facial bones? 3. Cranium-Sutures: a. Sagittal b. Coronal c. Squamosal d. Lambdoidal e. Wormian (sutural) bones: these are small islands of bone that fill gaps in sutures; they are not always present. Look in the lambdoidal sutures. 4. Cranium-Special Landmarks, processes, structures, foramina, fontanels a. Orbit: identify all of the bones in the orbit b. Mandibular fossa c. External acoustic (auditory) meatus d. Styloid, mastoid processes Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.18 e. Greater wing of sphenoid f. Fontanels (The anterior fontanel is easily visible.) 5. Cranium-Floor a. Bones (See your textbook.) b. Fossae: Anterior, middle, and posterior cranial fossae; hypophyseal(shallow depression in middle of sella turcica) c. Cribiform plate, cristae galli d. Sella turcica 1. Hypophyseal fossa 2. Dorsum sella(ridge on posterior aspect of sella) e. Carotid canal f. Jugular foramen g. Foramen Magnum h. Occipital Condyles 6. Face: Bones a. Maxilla b. Mandible c. Palatine-2 d. Zygomatics-2 e. Lacrimal-2 f. Nasals-2 g. Vomer h. Superior, Middle, & Inferior Nasal Conchae i. Mandible 7. Face: special processes, structures a. Alveoli(tooth sockets) b. Perpendicular plate of ethmoid c. Mandible: body, rami, coronoid process, mandibular condyle, angle d. Paranasal sinuses: frontal, maxillary, sphenoidal, ethmoid air cells e. Zygomatic arch 8. Spine: 33 separate bones in fetus & infant; in children & adults it's made of 26 bones: coccyx( 4 fused vertebrae), sacrum (5 fused vertebrae), & 24 individual vertebrae. Know number of vertebrae in each part of back (7 cervical, 12 thoracic, 5 lumbar) a. Direction of curves in cervical (concave posterior), thoracic (convex posterior), lumbar (concave posterior) regions 9. Structures of Typical Vertebra a. Body, vertebral foramen, spinous process, transverse process, superior & inferior articular processes b. vertebral arch: made of laminae & pedicles c. intervertebral foraminae between vertebrae d. transverse foraminae in cervical vertebrae e. facets: on super & inferior articular processes: smooth surfaces for joints between processes Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.19 10. Intervertebral Disk: annulus fibrosus (fibrocartilage), nucleus pulposus 11. Cervical vertebrae a. How many(7), direction of curve- concave posterior, note have foraminae in transverse processes: what for?. Note bifid spinous processes b. C1=atlas: superior articular facet for articulation with occipital condyle c. C2=axis(know odontoid process) 12. Thoracic Vertebrae a. How many-12, direction of curve-convex posterior b. long spinous processes, point inferiorly 13. Lumbar a. How many-5, direction of curve-concave posterior, have thick bodies& spinous processes 14. Sacrum: convex posterior, sacral promontory, sacroiliac joints 15. Coccyx-4 rudimentary vertabrae 16. Thoracic cage = Bony Thorax a. Sternum: 1. manubrium 2. sternal angle: separates manubrium from body 3. body 4. xiphoid process (cartilage when young, ossifies about age 40) b. Ribs: 1. true: 1st 7 pairs;, cartilage of rib(costal cartilage) articulates with sternum 2. false: 8-10, cartilage of rib articulates with cartilage above, while lowest 2 pairs are floating false ribs 17. Note how the ribs attach to the vertebrae. Looking at them from the posterior side of the back, there is an acute angle created between the 12th rib and the spine due to the downward direction of the 12th rib as it heads anteriorly. This angle is the costovertebral angle. Tenderness upon palpation of the costovertebral angle (performed with one finger) is a physical sign associated with pyelonephritis, which is a kidney infection. 18. Hyoid Bone: attachment for muscles of tongue, neck, & pharynx Part 3: Appendicular Skeleton : Upper & Lower extremities, Shoulder Girdle, Pelvic Girdle clavicle acromial and sternal ends scapula spine medial, lateral, & superior borders and angles (superior, lateral, inferior) acromion or acromial process coracoid process fossa (supraspinous, infraspinous & subscapular) Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.20 glenoid fossa (cavity) scapular notch humerus head greater & lesser tubercle intertubercular (bicipital groove or sulcus) anatomical & surgical necks deltoid tubercle groove for the radial nerve trochlea capitulum olecranon fossa medial & lateral epicondyles radial fossa & coronoid fossa supracondylar ridges (medial and lateral)—region proximal to each epicondyle ulna head of the ulna styloid process of the ulna olecranon process coranoid process semilunar or trochlear notch radial notch of the ulna interosseous ridge of the ulna radius radial head radial tubercle (tuberosity) ulnar notch of the radius interosseous ridge of the radius styloid process of the radius carpals a. proximal row, lateral to medial: scaphoid, lunate, triquetrum, pisiform b. distal row, lateral to medial: trapezium, trapezoid, capitate, hamate metacarpals phalanges proximal, middle, distal (on an articulated hand) os coxae (ilium, ischium, pubis), acetabulum, obturator foramen ilium spines (anterior-superior, anterior-inferior, posterior-superior, posteriorinferior) iliac crest; iliac fossa (on the anterior surface) body of the ilium auricular surface greater sciatic notch arcuate line inferior gluteal line Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.21 ischium ischial tuberosity spine (ischial or sciatic) body of the ischium superior & inferior rami lesser sciatic notch pubis symphysis, body of the pubis superior & inferior rami pubic tubercle pubic arch pubic angle femur head of the femur neck of the femur greater & lesser trochanter intertrochanteric line and crest linea aspera fovea capitus medial and lateral condyles of the femur adductor tubercle—located proximal to medial epicondyle medial & lateral epicondyles of the femur patellar surface intercondylar notch or fossa gluteal tubercle patella medial and lateral articular surfaces tibia medial & lateral condyles of the tibia intercondylar eminence tibial tuberosity fibular surface of the tibia diaphysis medial malleolus anterior crest of the tibia fibular notch tibular articular surface for the talus fibula head of the fibula styloid process (apex) of the fibula lateral malleolus fibular articular surface for the talus tarsals talus-bears body's weight, calcaneus, navicular, cuboid, 1st-3rd cuneiforms metatarsals, phalanges (proximal, middle, distal)—on articulated foot only Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.22 OTHER IMPORTANT STRUCTURES anterior, middle, and posterior cranial fossa—skull with calveria removed compact and cancellous bone pelvis—be able to distinguish between male & female and know characteristics of each type 1. Male pelvis: vertical iliac bones, pubic arch <90 degrees, deep iliac fossa, heart shaped pelvic inlet 2. Female pelvis: less vertical iliacs, arch >90, shallow iliac fossa, round pelvic brim(inlet) Part 4: Articulations and Body Movements (Exercise 13): 1. Knee a. b. c. d. medial & lateral collateral ligaments(=tibial & fibular collaterals) anterior & posterior cruciates medial & lateral menisci Quadriceps tendon, patellar ligament 2. Joint Movements: a. flexion and extension b. hyperextension c. dorsiflexion and plantar flexion of ankles d. abduction and adduction e. circumduction f. pronation and supination g. inversion and eversion Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.23 Muscles Study Guide (Exercise 14-15) and Nerve Histology (Exercise 17) 1. Complete the following table: Muscle type # nuclei per cell Visible striations locations voluntary or involuntary cell shape Cardiac Skeletal Smooth 2. histology of skeletal muscle a. cross section of muscle b. longitudinal section of individual cells c. sarcomere d. neuromuscular junction Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.24 MUSCLE ORIGIN INSERTION ACTION NERVE MUSCLES OF THE PECTORAL REGION AND ANTERIOR ABDOMINAL WALL Pectoralis major Lateral lip of intertubular groove of humerus Pectoralis minor Medial half of clavicle, sternum, costal cartilages, aponeurosis of external abdominal oblique Anterior surface of ribs 3 to 5 Subclavius Cartilage of first rib Inferior groove of clavicle Flexes, abducts, and medially rotates humerus, draws body upward in climbing Draws scapula down and forward and elevates ribs Draws shoulder down and forward External abdominal oblique External surface of lower 8 ribs Anterior half of iliac crest and linea alba Compresses abdomen, rotates and flexes vertebral column Internal abdominal oblique Lateral half of linguinal ligament, anterior iliac crest and thoracolumbar fascia Lateral third of inguinal ligament, anterior iliac crest, and thoracolumbar fascia Pubis symphysis and crest of pubis Lower four ribs, linea alba and by conjoined tendon to pubis Compresses abdomen, rotates and flexes vertebral column Linea alba, and by conjoined tendon to pubis Compresses abdomen and depresses ribs Xiphoid process and cartilages of ribs 5 to 7 Tenses abdominal wall and flexes vertebral column Transverse abdominis Rectus abdominus Coracoid process of scapula Lateral and medial pectoral Medial pectoral Nerve to subclavius Intercostals 8 to 12, iliohypogastric, ilioinguinal Intercostals 8 to 12, iliohypogastric, ilioinguinal Intercostals 7 to 12, iliohypogastric, ilioinguinal Intercostal 7 to 12 SUPERFICIAL MUSCLES OF THE BACK Trapezius Latissimus dorsi Levator scapula Rhomboid major Rhomboid minor Serratus anterior External occipital protuberance, superior nuchal line, nuchal ligament, and spinous process of cervical 7 through 12 Spinous processes of lower 6 thoracic vertebrae, thoracolumbar fascia, crest of ilium Transverse processes of cervical 1 through 4 Spinous process of thoracic 2 through 5 and supraspinous ligament Spinous processes of cervical 7 through thoracic 1 Lateral surface of upper 8 ribs Anterior border of scapular spine, acromion process, lateral third of posterior clavicle Floor of intertubercular groove of humerus Medial border above spine of scapula Medial border below spine of scapula Medial border of scapula at root of spine Anterior lip of medial border of scapula Adducts, rotates, and elevates scapula Spinal accessory XI, C3, and C4 Adducts, extends, rotates humerus medially, draws shoulder down and backward Draws scapula medially and depresses shoulder Adducts and laterally rotates scapula Thoracodorsal Adducts and laterally rotates scapula Dorsal scapular Holds scapula to chest wall, rotates scapula in raising humerus Long thoracic Doral scapular Dorsal scapular Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.25 MUSCLE ORIGIN INSERTION ACTION NERVE MUSCLES OF THE SHOULDER AND ARM Subscapularis Medial part of subscapular fossa Lesser tubercle of humerus Rotates humerus medially Supraspinatus Medial part of supraspinous fossa Abducts humerus Infraspinatus Medial part of infraspinous fossa Rotates humerus laterally Suprascapular Teres major Axillary border at inferior angle of scapula Axillary border of scapula Superior portion of greater tubercle of humerus Middle portion of greater tubercle of humerus Medial lip of intertubular groove of humerus Inferior portion of greater tubercle of humerus Deltoid tubercle of humerus Upper and lower subscapularis Suprascapular Lower subscapular Olecranon process of ulna Adducts, extends, and rotates humerus medially Laterally rotates humerus and adducts it Abducts humerus; aids in flexion, extension, and adduction Extends humerus and ulna Coronoid process of ulna Flexes ulna Musculocutaneous and radial Musculocutaneous Teres minor Deltoid Circumflex Triceps brachii Long head Lateral head Medial head Brachialis Anterior surface, lateral clavicle, acromion process and spine of scapula Long head, infraglenoid tubercle; lateral head, proximal portion of posterior humerus; medial head, distal half of posterior humerus Distal two-thirds of front of humerus Circumflex Coracobrachialis Coracoid process of scapula Middle third of humerus Flexes and adducts humerus Biceps brachii Long head Short head Anconeus Long head, supraglenoid tubercle; short head, coracoid process scapula Tuberosity of radius and bicipital aponeurosis Flexes radius and humerus, and supinates hand Musculocutaneous Lateral epicondyle of humerus Olecranon process, posterior surface of ulna Weak extensor of humerous Radial Radial MUSCLES OF ANT. FOREARM Pronator quadratus Flexor digitorum profundus Flexor pollicis longus Flexor digitorum superficialis Distal fourth of ulna Anterior and medial surfaces of ulna and interosseous membrane Middle half of radius, interosseous membrane, coronoid process of ulna Medial epicondyle of humerus and coronoid process of ulna Distal fourth of radius Distal phalanges of fingers Pronates hand Flexes fingers and wrist Median Median and ulnar Distal phalanx of thumb Flexes thumb Median Middle phalanges of fingers Flexes fingers and wrist Median Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.26 MUSCLE ORIGIN INSERTION Flexor carpi radialis Medial epicondyle of humerus Base of second metacarpal Flexor carpi ulnaris Medial epicondyle of humerus, olecranon process, & posterior ulna Medial epicondyle of humerus and coronoid process of ulna Pisiform, hamate, and fifth metacarpal Middle portion of radius Pronator teres ACTION NERVE Flexes wrist and elbow and abducts wrist Flexes and adducts wrist Median Pronates hand and flexes radius Median Ulna MUSCLES OF ANTEROLATERAL FOREARM & DORSUM OF HAND Extensor indicis Extensor pollicis longus Extensor pollicis brevis Abductor pollicis longus Supinator Brachioradialis Extensor carpi radialis longus Extensor carpi radialis brevis Extensor digitorum communis Extensor carpi ulnaris Exten. digiti minimi Posterior surface of ulna and interosseous membrane Middle third of ulna and interosseous membrane Middle third of radius and interosseous membrane Posterior surface of ulna and radius, and interosseous membrane Lateral epicondyle of humerus, supinator crest of ulna Lateral supracondylar ridge and lateral intermuscular septum Lateral supracondylar ridge of humerus and intermuscular septem Lateral epicondyle of humerus Dorsal extensor expansion of index finger Base of distal phalanx of thumb Extends index finger Radial Extends thumb and abducts hand Radial Base of proximal phalanx of thumb Extends thumb and abducts hand Radial First metacarpal Abducts thumb and hand Radial Lateral surface and posterior border of radius Styloid process of radius Supinates hand Radial Flexes radius Radial Second metacarpal Extends and abducts hand Radial Third metacarpal Extends and abducts wrist Radial Lateral epicondyle of humerus Into distal phalanx by 4 tendons Extends fingers and wrist joint Radial Lateral epicondyle of humerus and posterior border of ulna Lateral epicondyle of humerus Fifth metacarpal Extends and adducts hand Radial Extensor expansion of little finger Extends little finger Radial MUSCLES OF THE PALMAR SURFACE OF THE HAND MUSCLE Flexor pollicis brevis Abductor pollicis brevis Flexor digiti minimi ORIGIN Flexor retinaculum and trapezium Flexor retinaculum, scaphoid and trapezium Flexor retinaculum and hook of hamate INSERTION ACTION NERVE Proximal phalanx of thumb Proximal phalanx of thumb Flexes thumb Abducts thumb and aids in flexion Median Median Proximal phalanx of fifth digit Flexes fifth digit Ulnar Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.27 MUSCLE Abductor digiti minimi ORIGIN INSERTION Pisiform and tendon of flexor carpi ulnaris Proximal phalanx of fith digit ACTION NERVE Abducts fifth digit Ulnar Obturator MUSCLES OF ANT. HIP AND THIGH Adductor brevis Inferior pubic ramus Adductor magnus Pubic arch and ischial tuberosity Upper part of linea aspera and pectineal line Linea aspera and adductor tubercle Adductor longus Between pubic rami near symphysis Middle third of linea aspera Pectineus Gracilis Quadriceps femoris Rectus femoris Vastus lateralis Pectineal line of pubis Inferior pubis near symphysis Anterior inferior iliac spine and upper margin of acetabulum Intertrochanteric line and linea aspera of femur Intertrochanteric line and linea aspera of femur Upper shaft of femur Anterior superior iliac spine Iliac crest Pecinteal line of femur Upper portion of tibia Tibial tuberosity Adducts, flexes, and medially rotates femur Adducts, flexes, and laterally and medially rotates femur Adducts, flexes & medially rotates femur Adducts femur Adducts, flexes, medially rotates tibia Extends tibia and flexes femur Tibial tuberosity Extends tibia Femoral Tibial tuberosity Extends tibia Femoral Tibial tuberosity Medial margin of tibial tuberosity Iliotibial tract Extends tibia Flexes both femur and tibia Tenses fascia lata Femoral Femoral Superior gluteal Vastus medialia Vastus intermedius Sartorius Tensor fasciae latae Obturator & sciatic Obturator Obturator femoral Obturator Femoral MUSCLES OF THE GLUTEAL REGION AND POSTERIOR THIGH Gluteus minimis Lower portion of ilium Piriformis Pelvic surface of sacrum Gluteus medius Middle portion of ilium Greater trochanter and capsule of hip joint Greater trochanter of femur Abducts and medially rotates femur Superior gluteal Rotates femur laterally Oblique ridge on greater trochanter of femur Abducts and medially rotates femur Twigs from sacral one and two Superior gluteal Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.28 MUSCLE Gluteus maximus Biceps femoris Long head Short head Semimembranosus Semitendinosus ORIGIN INSERTION ACTION Upper portion of ilium, the sacrum and coccyx Long head, ischial tuberosity; short head, supracondylar ridge of femur Gluteal tuberosity and iliotibial tract Principal extensor and powerful rotator of femur Flexes leg and rotates leg laterally; long head extends femur Inferior gluteal Ischial tuberosity Medial condyle of tibia Sciatic Ischial tuberosity, fused with long head of biceps Tuberosity of tibia Extends thigh, flexes and medially rotates tibia Extends thigh and flexes tibia Head of fibula and lateral condyle of tibia NERVE Sciatic Sciatic INTERNAL MUSCLES OF THE THIGH & GLUTEAL REGIONS Quadratus lumborum Iliolumbar ligament and crest of ilium Psoas Major Transverse processes of bodies of lumbar vertebrae Lower border of 12th rib, transverse process of upper lumbar vertebrae Lesser trochanter of femur with iliacus Depresses rib cage inferiorly and abducts trunk to same side Flexes and laterally rotates femur Nerve to quadratus lumborum Major by 2nd and 3rd lumbar Flexes vertebral column Minor Iliacus Bodies of 12th thoracic and 1st lumbar vertebrae Iliac fossa and lateral margin of sacrum Gemelli Superior Inferior Obturator internus Ischial spine Quadratus femoris Ischial tuberosity Margin of obturator foramen Pectineal line on ilium Minor by 1st lumbar Femoral Lesser trochanter of femur with psoas major Tendon of obturator internus to greater trochanter Flexes and laterally rotates femur Rotates femur laterally Twigs from sacral plexus Medial surface of greater trochanter of femur Greater trochanter and shaft of femur Rotates femur medially and aids abduction Rotates femur laterally Twigs from lumbar 5 and sacral 1 Twigs from lumbar 4 and 5, and sacral 1 Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.29 MUSCLE ORIGIN INSERTION ACTION NERVE MUSCLES OF THE POSTERIOR LEG Tibialis posterior Interosseous membrane and tibia and fibula on either side Flexor hallucis longus Distal two-thirds of fibula and intermuscular septum Middle half of tibia Flexor digitorum longus Popliteus Plantaris Soleus Gastrocnemius Lateral condyle of femur Popliteal surface of femur Upper third of fibula and soleal line of tibia Medial and lateral condyles of femur Navicular, with slips to cuneiform; cuboid; second, third, and fourth metatarsals Distal phalanx of great toe Adducts and inverts foot and aids in plantar flexion Tibial Flexes great toe Tibial By four tendons into distal phalanges of lateral four toes Flexes lateral four toes Tibial Posterior tibia above soleal line Medial calcaneus With gastrocnemius into calcaneus via calcaneal tendon With soleus into calcaneus via calcaneal tendon Flexes tibia and rotates femur laterally Flexes foot Flexes foot Tibial Tibial Tibial Flexes tibia and plantar; flexes foot Tibial MUSCLES OF THE ANTEROLATERAL LEG AND DORSUM OF THE FOOT Tibialis anterior Extensor hallucis longus Extensor digitorum longus Peroneus tertius (fibularis) Peroneus brevis (fibularis) Peroneus longus (fibularis) Extensor digitorum brevis Extensor hallucis brevis Upper half of tibia and interosseous membrane Middle half of fibula and interosseous membrane Tibia, proximal three-fourths of fibula, & interosseous membrane Distal third of fibula and interosseos membrane Lower two-thirds of fibula Upper two-thirds of fibula and intermuscular septa Dorsal surface of calcaneus Distal lateral surface of calcaneus First cuneiform and first metatarsal Extends and inverts foot Deep peroneal Distal phalanx of great toe Extends great toe Deep peroneal Tendons to middle & terminal phalanges of four lateral toes by extensor expansion Fifth metatarsal Extends toes Deep peroneal Extends and everts foot Deep peroneal Fifth metatarsal Everts and abducts foot and aids in plantar flexion Everts and aids in plantar flexion Extends toes Superficial peroneal Superficial peroneal Deep peroneal Extends toes Deep peroneal First cuneiform and first metatarsal By four tendons into extensor expansion Proximal phalanx of great toe Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.30 MUSCLE ORIGIN INSERTION ACTION NERVE MUSCLES OF THE SOLE OF THE FOOT Flexor hallucis brevis Abductor hallucis Quadratus plantae Flexor digitorum brevis Flexor digiti minimi brevis Abductor digiti minimi Navicular and cuneiform bones, and long plantar ligament Calcaneus and plantar aponeurosis Calcaneus and long plantar ligament Calcaneus and plantar aponeurosis Fifth metatarsal and sheath of peroneus longus tendon Calcaneus and plantar aponeurosis 2 tendons to medial & lateral sides of proximal phalanx of great toe Proximal phalanx of great toe Into tendons of flexor digitorum longus By four tendons into middle phalanx of lateral four toes Proximal phalanx of little toe Flexes great toe Flexes little toe Lateral plantar Proximal phalanx of little toe Abducts little toe Lateral plantar Abducts great toe Supports action of tendon of flexor digitorum longus Flexes lateral four toes Medial and lateral plantar Medial plantar Lateral plantar Medial plantar MUSCLES OF THE FACE Buccinator Depressor anguli oris Depressor labii inferioris Frontalis Levator anguli oris Mentalis Nasalis Orbicularis oculi Orbicularis oris Zygomaticus Temporalis Masseter Pterygomandibular raphe and alveolor processes of mandible and maxilla Oblique line of mandible Mandible next to mental foramen Angle of mouth into orbicular orbis Compresses cheek during mastication Facial VII Angle of mouth Lower lip Draws angle of mouth up and back Draws lower lip down Facial VII Facial VII Epicranial aponeurosis Skin over forehead Facial VIi Maxilla adjacent to canine fossa Incisor fossa of mandible Maxilla adjacent to canine and incisor teeth Medial orbital margin, medial palpebral ligament and zygomatic bones By muscular skips to surround muscles Zygomatic arch Temporal fossa and fascia Zygomatic process and arch Corner of mouth Skin over chin Side of nose above nostril Elevates eyebrows and wrinkles skin of forehead Elevates corner of mouth Elevates and protrudes lip Draws margin of nostril towards septum Closes eyelids and depresses skin of forehead Closes and purses lips Elevates corner of mouth Elevates and retracts mandible Elevates mandible and closes jaw Skin surrounding eye, and lateral palpebral ligament Muscles interlace to surround mouth Corner of mouth Coronoid process of mandible Angle and ramus of mandible Facial VII Facial VII Facial VII Facial VII Facial VII Facial VII Trigeminal V Trigeminal V Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.31 SUPERFICIAL MUSCULATURE OF THE NECK MUSCLE ORIGIN INSERTION Omohyoid Thyrohyoid Body of hyoid bone Oblique line of thyroid cartilage Medial tip of suprascapular notch Greater horn of hyoid bone Sternothyroid Sternohyoid Posterior surface of manubrium Posterior surface of manubrium, and medial clavicle Styloid process Mylohyoid line of mandible Oblique line of thyroid cartilage Hyoid bone Lower border of mandible near midline Intermediate tendon Mastoid notch of temporal bone Intermediate tendon Manubrium and medial third of clavicle Mastoid process Stylohyoid Mylohyoid Digastric (biventer) anterior belly Digastric (biventer) Posterior belly Sternocleidomastoid Hyoid bone Hyoid bone and median raphe ACTION NERVE Depresses hyoid bone Depresses hyoid bone, elevates thyroid cartilage Depresses thyroid cartilage Depresses hyoid bone Ansa cervicalis Ansa cervicalis Moves hyoid bone up and back Elevates hyoid bone and floor of mouth, depresses mandible Elevates hyoid bone and base of tongue, depresses mandible Moves hyoid bone back Facial VII Trigeminal V Rotates head to same side, elevates chin, and flexes vertebral column C2 and C2, spinal accessory, XI Ansa cervicalis Ansa cervicalis Trigeminal V Facial VII DEEP MUSCLES OF THE BACK Erector Spinae (Sacrospinalis) --iliocoastalis thoracicus lumborum --longissimus dorsi --spinalis dorsi Sacrum, iliac crest, spinous processes of lumbar vertebrae and T11,12 Angle of the last 6 or 7 ribs Extension of vertebral column Spinal nerves dorsal rami Sacrum, iliac crest, spinous processes of lumbar vertebrae and T11,12 Sacrum, iliac crest, spinous processes of lumbar vertebrae and T11,12 Transverse processes of thoracic vertebrae Spinous processes of upper thoracic vertebrae Extension of vertebral column Spinal nerves dorsal rami Spinal nerves dorsal rami Extension of vertebral column MUSCLES OF THORACIC WALL External intercostal Internal intercostal Inferior border of rib above Superior border of rib below Superior border of rib below Inferior border of rib above Elevates rib cage/inspiration Depresses rib cage/expiration Intercostal Intercostal Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.32 Exercise 17: Histology of Nervous Tissue 3. Know the basic cell structure of a multipolar neuron: a. Cell body b. Nucleus c. Nucleolus d. Axon hillock e. Dendrite f. Axon g. Schwann cells and myelin sheath h. Nodes of Ranvier i. Telodendria j. Axon Terminals=Synaptic end bulbs 4. 5. 6. 7. 8. Distinguish between unipolar, bipolar, and multipolar neurons What is another word for a sensory neuron? What is another word for a motor neuron? What are association, or interneurons? Learn the histology of nerves, and identify the following structures on a microscope slides; drawings; and models if available in lab: a. epineurium b. perineurium c. endoneurium d. fascicle e. axon f. myelin sheath Biology 231, Anatomy and Physiology I, Sylvania Laboratory Survival Guide p.33