* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Review Problems #2 (Enzyme Review, Phosphatases
Biochemical cascade wikipedia , lookup
Photosynthesis wikipedia , lookup
Biosequestration wikipedia , lookup
Microbial metabolism wikipedia , lookup
Photosynthetic reaction centre wikipedia , lookup
Catalytic triad wikipedia , lookup
Nucleic acid analogue wikipedia , lookup
Ribosomally synthesized and post-translationally modified peptides wikipedia , lookup
Butyric acid wikipedia , lookup
Isotopic labeling wikipedia , lookup
Point mutation wikipedia , lookup
Proteolysis wikipedia , lookup
Fatty acid metabolism wikipedia , lookup
Fatty acid synthesis wikipedia , lookup
Protein structure prediction wikipedia , lookup
Peptide synthesis wikipedia , lookup
Metalloprotein wikipedia , lookup
Citric acid cycle wikipedia , lookup
Genetic code wikipedia , lookup
Biochemistry wikipedia , lookup
Review Problems (Amino acid Biosynthesis) Week 10 1) The carbons of phenylalanine and tyrosine are derived from what glucogenic molecules? 2) The branchpoint for aromatic amino acid biosynthesis is chorismate. What is the structure of chorismate? What are the three immediate products derived from chorismate that constitute the first unique steps in the synthesis of the three aromatic amino acids? 3) From where are the two carbons of the five-membered ring of tryptophan derived? Be specific. (Show the molecule and highlight the carbons that are used.) The carbons that are not used are converted into what compound? 4) The nonaromatic carbons of tryptophan are derived from what precursor? What cofactor is involved in this reaction? Show the mechanism of this transformation. 5) Roundup (glyphosate) inhibits biosynthesis of aromatic amino acids. Which step does it inhibit? Why does this inhibitor have little effect on humans? Considering that glyphosate is an incredibly effective herbicide, can you give a likely reason for why this compound is not an effective antibiotic. 6) Which transformation in aromatic amino acid biosynthesis requires NAD+, but does not yield NADH as a product. Explain the role of this cofactor. (Hint: this is similar to the reaction catalyzed by S-adenosylhomocysteine hydrolase). 7) What are the carbon-containing precursors used for the synthesis of valine and isoleucine? What cofactor is common to both these biosynthetic pathways? Explain its role and draw a mechanism for the transformation. 8) The biosynthesis of valine and isoleucine have a rearrangement step that is very similar. Draw this rearrangement (curly arrows please). 9) Leucine is derived from the same pathway that generates valine. What additional carbon source is used in this synthesis? The later stages of leucine’s biosynthetic pathway are similar to what other pathway? 10) Threonine is derived from what non-proteinacious amino acid? Which common amino acid is the ultimate source of this molecule? 11) The methyl group of methionine is derived from what common amino acid? What is the byproduct of this one carbon transfer? What cofactor catalyzes this carbon cleavage? What is the acceptor of this carbon? The actual agent that ultimately transfers the carbon is which cofactor? 12) Alpha-ketoglutarate provides the carbon skeleton for which amino acids? 13) Two different amidation methods are used to install side chain amides in amino acids. Describe these two methods and match them to the relevant amino acid. Why do you think this difference exists? Where are similar activation strategies employed in biosynthesis? 14) Which amino acids derive their carbon skeletons completely from oxaloacetate? 15) Which amino acid is derived from oxaloacetate and pyruvate? What carbon piece is lost in this process? What cofactor plays a role in this process? Draw a general mechanism for this reaction. What molecule plays a role as a protecting group in this pathway? 16) Serine is derived from which glucogenic precursor? 17) Inhibition of a key enzyme activity by the end product of a biosynthetic pathway is known as what? 18) Why is it useful to have multiple isozymes of enzymes that comprise common pathways to multiple amino acids? 19) What molecules provide the carbon and nitrogen sources for histidine. Please delineate which atoms in the starting materials wind up in which places in histidine. 20) What enzyme is responsible for the fixation of nitrogen? 21) How many electrons are required to reduce nitrogen gas to ammonia? How many electrons are required in the biological reduction? If these are not the same, explain why. 22) What are the ultimate biological molecules that act as acceptors of ammonia in higher organisms? What enzymes mediate this(ese) transformation(s)? What are the energetic requirements for this(ese) reaction(s)? 23) What nine amino acids cannot be synthesized in humans? 24) Draw the degradative pathways for threonine. 25) What cofactor is used for hydroxylation of phenylalanine. Draw the mechanism of this reaction. 26) Outline the degradative pathway for phenyalanine. 27) Outline the degradative pathway for lysine. 28) The indole ring of trp is degraded into which molecule? What amino acid is derived from the first three carbons of trp? How does this explain whether trp is ketogenic or glucogenic?