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Molecular Biology Medicine Part I SWITH Ettore Sansavini Health Science Foundation – ONLUS Lugo (Ravenna), Italy Carlo Ventura Professor of Molecular Biology University of Bologna, Italy Now please shift to the cell world and Regenerative Medicine Body regeneration • We regenerate most of our body approximately every 3 months. • So, our body has inherent regeneration potential and we are naturally targeted to self-healing • Moreover a population of resident stem cells, that can drive a regeneration process, is distributed across the body tissues Each individual is a small piece of nature • We live in a multitude of physical signals and fields: electromagnetic fields, Higgs field, gravitational field…… • The human body is unceasingly penetrated and crossed by a number of cosmic particles, energy waves, electrically charged or not. Each individual is a small piece of nature Our body generates a seeming infinity of rhythms and vibrations expressing the cells’ molecular dynamics, now recordable at the atomic and molecular levels. Different cells may have different languages. If you record a culture of healthy cells, old cells, cancer cells you can capture different languages. Through these reciprocal physical signals, (besides many biological messages), the cells are able to organize their decisions (activation of specific functions, migration, differentiation….. apoptosis) We live in an active physical environment In conclusion, the body’s cells both generate electrical microcurrents and are sensitive to external physical fields and acoustic or subsonic vibrations, and can react with profound functional changes, including trans-differentiation, until clocking back to a time zero in which all kind of decisions are still possible Novel Findings Reprogramming human adult stem cells and human non-stem somatic cells (skin fibroblasts) into embryonic like stem cells, capable to differentiate along cardiogenic, vasculogenic, skeletal myogenic and neurogenic lineages Reversing human Stem Cell Aging Reprogramming Cancer Stem Cells Ultimate goal To reprogram the tissue resident stem cells with no need for stem cell/tissue transplantation, by a: non-invasive, inexpensive, personalized, and easily deliverable on large-scale bases strategy. Cells Have a Vision Cells are able to detect the orientation of others by signals that penetrated glass but not a thin metallic film. These signals are carried by electromagnetic radiation FROM THE CELL SURFACE TO NUCLEUS, A WORLD OF COHERENT VIBRATIONS Labeling with fluorescent tubulin Released MTs move away from the12 centrosome, out of the nuclear region and translocate to the cytoplasm Sahu S et al, 2013 Sahu S et al, 2013 New Concepts on Biomolecular Recognition All Life is About Rhythms and Their Timely Patterning 17 18 Symphony of life, revealed: New imaging technique captures vibrations of proteins, tiny motions critical to human life Like the strings on a violin or the pipes of an organ, the proteins in the human body vibrate in different patterns, scientists have long suspected. Now, a new study provides what researchers say is the first conclusive evidence that this is true. This graphic visualizes the vibrations in lysozyme as it is excited by terahertz light (depicted by the red wave arrow). Such vibrations, long thought to exist, have never before been described in such detail, said lead researcher Andrea Markelz, ( University of Buffalo). terahertz near-field microscopy NATURE COMMUNICATIONS | ARTICLE Optical measurements of long-range protein vibrations Gheorghe Acbas, Katherine A. Niessen, Edward H. Snell & A.G. Markelz Nature Communications 5, Article number: 3076 doi:10.1038/Published 16 January 2014 a singular vibrational entity in a web makes it exhibiting connectedness 20 Atomic Force Microscopy (AFM) Single Kinesin Imaging on a Single Microtubule Schaap IAT et al, 2011 Connectedness is an Evolving Form of Recognition Masha Dimitri Equistasi consists in a rectangular plate measuring 1062060.5 mm and with a weight of 0.17 gr (Figure 1). The device is exclusively composed by nanotechnology fibers that transform the body temperature into mechanical vibratory energy (<0.8N, 9000 Hz) able to generate a variation of muscle length of max 0.02 mm [36], by far within the safety limit (0.12 mm) found to be harmful for human muscles Interrogation of Nanomechanical Signatures During Stem Cell Development (“Nanomechanical characterization of cellular activity - Sonocytology”) Vibrations arise from the integration of various oscillatory rhythms, from nanomechanical properties of sub cellular structures up to the cell surface Sound from single yeast cell 22 °C 26 °C 30 °C Dead cell In both the philosophical and visual sense, ‘seeing is believing’ does not apply to the emerging new discoveries in Science, for which there is nothing even remotely visible to create proof of existence. On the atomic and molecular scale, data are recorded by sensing and probing in a very abstract manner, which requires complex and approximate interpretations. We are now facing a novel path where visualization and creation of a narrative becomes necessary to describe what is sensed, not seen. We have growing needs of separating the “informational content” of Life from its “material substrate.” “Information” and is deployment into a multifaceted “knowledge” are the essence of Life. SWITH (Stem Wave Institute for Tissue Healing), Ettore Sansavini Health Science Foundation, Lugo (Ravenna), Italy Carlo Ventura Francesca Bianchi Claudia Cavallini Elena Olivi Riccardo Tassinari SONOLAB, Bologna, Italy Maurizio Villa Rinaldi-Fontani Institute, Florence, Italy Salvatore Rinaldi Vania Fontani Alessandro Castagna Department of Biomedical Sciences University of Sassari, Italy Margherita Maioli Valentina Basoli Sara Santaniello Gianfranco Pigliaru Sara Gualini Department of Chemistry and Biochemistry UCLA, LA, USA James K. Gimzewski Sarah Cross Andrew Pelling Pasteur Institute, Paris UNESCO, Paris Luc Montagnier Cell Transplant Center Diabetes Research Institute (DRI) WorldWide DRI Federation University of Miami, USA Camillo Ricordi Carlo Tremolada INFN, Italy Emilio del Giudice Giuseppe Vitiello Alberto Tedeschi