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Transcript
AS Biology Unit 1
page 1
Edexcel AS Biology Teacher 1
Contents
Specification
Biological Molecules
DNA
Viruses
Cell Division
Sexual Reproduction
Classification and Evolution
Water
Carbohydrates
Lipids
Proteins
Enzymes
DNA
Gene Expression
Gene Mutations
Viruses
Viral Diseases
Cell cycle and Mitosis
Meiosis
Chromosome Mutations
Sexual reproduction in Mammals
Sexual reproduction in Plants
Classification
Natural Selection
Speciation
Biodiversity
These notes may be used freely by A level biology students and teachers,
and they may be copied and edited.
Please do not use these materials for commercial purposes.
I would be interested to hear of any comments and corrections.
Neil C Millar ([email protected])
Head of Biology, Heckmondwike Grammar School
High Street, Heckmondwike, WF16 0AH
July 2015
HGS Biology A-level notes
NCM/2/16
AS Biology Unit 1
page 2
Biology Teacher 1 Specification
1.01 Water
The importance of the dipole nature of water leading
to hydrogen bonding and the significance of the
following to organisms: high specific heat capacity;
polar solvent; surface tension; incompressibility;
maximum density at 4 °C.
The structure of DNA, including the structure of the
nucleotides (purines and pyrimidines), base pairing, the
two sugar-phosphate backbones, phosphodiester
bonds and hydrogen bonds. How DNA is replicated
semi-conservatively, including the role of DNA
helicase, polymerase and ligase.
1.02 Carbohydrates.
The
difference
between
monosaccharides,
disaccharides and polysaccharides. The structure of
the hexose glucose (alpha and beta) and the pentose
ribose. How monosaccharides (glucose, fructose,
galactose) join to form disaccharides (sucrose, lactose
and maltose) and polysaccharides (starch formed from
amylose and amylopectin; glycogen) through
condensation reactions forming glycosidic bonds, and
how these can be split through hydrolysis reactions.
How the structure of glucose, starch, glycogen and
cellulose relates to their function.
1.07 Gene Expression
A gene is a sequence of bases on a DNA molecule
coding for a sequence of amino acids in a polypeptide
chain. The structure of mRNA including nucleotides,
the sugar phosphate backbone and the role of
hydrogen bonds. The structure of tRNA, including
nucleotides, the role of hydrogen bonds and the
anticodon.
1.03 Lipids
How a triglyceride is synthesised, including the
formation of ester bonds during condensation
reactions between glycerol and three fatty acids. The
differences between saturated and unsaturated lipids.
How the structure of lipids relates to their role in
energy storage, waterproofing and insulation. How
the structure and properties of phospholipids relate to
their function in cell membranes.
1.04 Proteins
The structure of an amino acid (structures of specific
amino acids are not required). The formation of
polypeptides and proteins (as amino acid monomers
linked by peptide bonds in condensation reactions).
The role of ionic, hydrogen and disulphide bonding in
the structure of proteins. The significance of the
primary, secondary, tertiary and quaternary structure
of a protein in determining the properties of fibrous
and globular proteins, including collagen and
haemoglobin. How the structure of collagen and
haemoglobin are related to their function.
1.05 Enzymes
Enzymes are catalysts that reduce activation energy.
Enzymes catalyse a wide range of intracellular
reactions as well as extracellular ones. The structure
of enzymes as globular proteins. The concepts of
specificity and the induced fit hypothesis.
How the initial rate of enzyme activity can be
measured and why this is important. Temperature,
pH, substrate and enzyme concentration affect the
rate of enzyme activity. Enzymes can be affected by
competitive, non-competitive and end-product
inhibition.
1.06 DNA
HGS Biology A-level notes
The nature of the genetic code, including triplets
coding for amino acids, start and stop codons,
degenerate and non-overlapping nature, and that not
all the genome codes for proteins. The processes of
transcription in the nucleus and translation at the
ribosome, including the role of sense and anti-sense
DNA, mRNA, tRNA and the ribosomes.
1.08 Gene Mutations
The term gene mutation as illustrated by base
deletions, insertions and substitutions. The effect of
point mutations on amino acid sequences, as
illustrated by sickle cell anaemia in humans.
1.09 Viruses
The classification of viruses is based on structure and
nucleic acid types as illustrated by λ (lambda) phage
(DNA), tobacco mosaic virus and Ebola (RNA) and
human immunodeficiency virus (RNA retrovirus). The
lytic cycle of a virus and latency.
1.10 Viral Diseases
Viruses are not living cells and so antivirals must work
by inhibiting virus replication. as viruses can be
difficult to treat once infection has occurred, the focus
of disease control should be on preventing the spread,
as exemplified by the 2014 Ebola outbreak in West
Africa. Be able to evaluate the ethical implications of
using untested drugs during epidemics.
1.11 Cell Cycle and Mitosis
The cell cycle is a regulated process in which cells
divide into two identical daughter cells, and that this
process consists of three main stages: interphase,
mitosis and cytokinesis. What happens to genetic
material during the cell cycle, including the stages of
mitosis. Mitosis contributes to growth, repair and
asexual reproduction.
1.12 Meiosis
Meiosis results in haploid gametes, including the stages
of meiosis. Meiosis results in genetic variation through
NCM/2/16
AS Biology Unit 1
recombination of alleles, including
assortment and crossing over.
page 3
independent
1.13 Chromosome Mutations
What chromosome mutations are, as illustrated by
translocations. How non-disjunction can lead to
polysomy, including Down’s syndrome, and
monosomy, including Turner’s syndrome.
1.14 Sexual Reproduction in Humans
The processes of oogenesis and spermatogenesis. The
events of fertilisation from the first contact between
the gametes to the fusion of nuclei. The early
development of the embryo to blastocyst stage.
1.15 Sexual Reproduction in Plants
How a pollen grain forms in the anther and the
embryo sac forms in the ovule. How the male nuclei
formed by division of the generative nucleus in the
pollen grain reach the embryo sac, including the roles
of the tube nucleus, pollen tube and enzymes. The
process of double fertilisation inside the embryo sac
to form a triploid endosperm and a zygote.
1.16 Classification
The limitations of the definition of a species as a group
of organisms with similar characteristics that
interbreed to produce fertile offspring. Why it is
often difficult to assign organisms to any one species
or to identify new species.
DNA sequencing and bioinformatics can be used to
distinguish
between
species
and
determine
evolutionary relationships. How gel electrophoresis
can be used to distinguish between species and
determine evolutionary relationships.
HGS Biology A-level notes
The classification system consists of a hierarchy of
domain, kingdom, phylum, class, order, family, genus
and species. The evidence for the three-domain model
of classification as an alternative to the five-kingdom
model and the role of the scientific community in
validating this evidence.
1.17 Natural selection
Organisms occupy niches according to physiological,
behavioural and anatomical adaptations. Evolution can
come about through natural selection acting on
variation bringing about adaptations. Reproductive
isolation can lead to allopatric and sympatric
speciation. The role of scientific journals, the peer
review process and scientific conferences in validating
new evidence supporting the accepted scientific
theory of evolution. There is an evolutionary race
between pathogens and the development of medicines
to treat the diseases they cause.
1.18 Biodiversity
Biodiversity can be assessed at different scales:
 within a habitat at the species level using a formula
to calculate an index of diversity
 within a species at the genetic level by looking at
the variety of alleles in the gene pool of a
population.
1.19 Conservation
The ethical and economic reasons (ecosystem
services) for the maintenance of biodiversity. The
principles of ex-situ (zoos and seed banks) and in-situ
conservation (protected habitats), and the issues
surrounding each method.
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BLANK PAGE
HGS Biology A-level notes
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AS Biology Unit 1
page 3
Biological Molecules
Living things are made up of thousands and thousands of different chemicals. These chemicals are called
organic because they contain the element carbon. In science organic compounds contain carbon–carbon
bonds, while inorganic compounds don’t. There are four important types of organic molecules found in
living organisms: carbohydrates, lipids, proteins, and nucleic acids (DNA). These molecules are mostly
polymers, very large molecules made up from very many small molecules, called monomers. Between them
these four groups make up 93% of the dry mass of living organisms, the remaining 7% comprising small
organic molecules (like vitamins) and inorganic ions.
Group name
Elements
Monomers
Polymers
% dry mass of a cell
Carbohydrates
CHO
monosaccharides
polysaccharides
15
Lipids
CHOP
fatty acids + glycerol*
triglycerides*
10
Proteins
CHONS
amino acids
polypeptides
50
Nucleic acids
CHONP
nucleotides
polynucleotides
18
* Triglycerides are not polymers, since they are formed from just four molecules, not many (see p10).
We'll study each of these groups in turn.
Chemical Bonds
In biochemistry there are three important types of chemical bond.
Covalent bonds are strong. They are the main bonds holding the atoms together in
the organic molecules in living organisms. Because they are strong, covalent bonds
don’t break or form spontaneously at the temperatures found in living cells. So in
biology covalent bonds are always made or broken by the action of enzymes.
Covalent bonds are represented by solid lines in chemical structures.
Ionic Bonds are fairly strong. They are formed between a positive ion (such as
NH+3 ) and a negative ion (such as
COO ). They are not common in biology since
ionic compounds dissociate in solution, but ionic bonds are sometimes found inside
protein molecules.
Hydrogen bonds are much weaker. They are formed between an atom (usually
hydrogen) with a slight positive charge (denoted +) and an atom (usually oxygen
or nitrogen) with a slight negative charge (denoted –). Because hydrogen bonds
are weak they can break and form spontaneously at the temperatures found in
living cells without needing enzymes. Hydrogen bonds are represented by dotted
lines in chemical structures.
HGS Biology A-level notes
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page 4
Water
Life on Earth evolved in the water, and all life still depends on water. At least 80% of the total mass of living
organisms is water. Water molecules are a charged dipole, with the oxygen atom being slightly negative (-)
and the hydrogen atoms being slightly positive (+). These opposite charges attract each other, forming
hydrogen bonds that bind water molecules loosely together.
This dipole property of water gives it many specific properties that have important implications in biology.
1. Water is an extremely good solvent. The water dipoles will stick to the atoms in almost all
crystalline solids, causing them to dissolve. Substances are often transported around living organisms as
solutes in aqueous solution (e.g. in blood or sap) and almost all the chemical reactions of life take place
in solution.

Charged or polar molecules such as salts, sugars, amino acids dissolve readily in water and so are
called hydrophilic ("water loving").

Uncharged or non-polar molecules such as lipids do not dissolve so well in water and are called
hydrophobic ("water hating").
Many important biological molecules ionise when they dissolve (e.g. acetic acid
acetate- + H+), so
the names of the acid and ionised forms (acetic acid and acetate in this example) are often used loosely
and interchangeably, which can cause confusion. You will come across many examples of two names
referring to the same substance, e.g. phosphoric acid and phosphate, lactic acid and lactate, citric acid
and citrate, pyruvic acid and pyruvate, aspartic acid and aspartate, etc. The ionised form is the one found
in living cells.
2. Water has a High Specific Heat. Water has a high specific heat capacity, which means that it takes a
lot of energy to heat, so water does not change temperature very easily. This minimises fluctuations in
temperature inside cells, and it also means that sea temperature is remarkably constant.
3. Water has a High Latent Heat. Water requires a lot of energy to change state from a liquid into a
gas, since so many hydrogen bonds have to be broken. So as water evaporates it extracts heat from
around it, and this is used to cool animals (sweating and panting) and plants (transpiration). Water also
must lose a lot of heat to change state from a liquid to a solid. This means it is difficult to freeze water,
so ice crystals are less likely to form inside cells.
HGS Biology A-level notes
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AS Biology Unit 1
page 5
4. Water is cohesive and adhesive.

Cohesion means that water molecules "stick together" due to their hydrogen bonds. This explains
why long columns of water can be sucked up tall trees by transpiration without breaking. It also
explains surface tension, which allows small animals to walk on water.

Adhesion means that water molecules stick to other surfaces, such as xylem vessels. This explains
capillary action (where water will be drawn along a narrow tube) and the meniscus on test tube
walls.
5. Water is most dense at 4°C. Most substances get denser as they cool down, and the solid form is
denser than the liquid form. Water is unique in that the solid state (ice) is less dense that the liquid
state, and in fact water is most dense at 4°C. This property causes several important effects:

Ice floats on water, so as the air temperature cools, bodies of water freeze from the surface,
forming a layer of ice with liquid water underneath. The surface ice layer acts as an insulator so
the water underneath does not freeze, allowing aquatic organisms to survive when the ambient
temperature is below zero, and even throughout long ice ages.

The expansion of water as it freezes causes freeze-thaw erosion of rocks, which results in the
formation of soil, without which there could be no terrestrial plant life.

Cold water sinks below warm water, and warm water rises above cold water, which gives rise
to many ocean currents.
6. Water is incompressible. The hydrogen bonds hold water molecules closer together than other
liquids, so water is very incompressible, since the molecules can’t be pushed any closer. So if a force is
applied to water, the water will move rather than squash, which allows blood to be pumped round a
body. The incompressibility is also used to make plant cells turgid and give eyes their shape.
HGS Biology A-level notes
NCM/2/16
AS Biology Unit 1
page 6
Carbohydrates
Carbohydrates contain only the elements carbon, hydrogen and oxygen. The group includes monomers,
dimers and polymers, as shown in this diagram:
Monosaccharides
Monosaccharides all have the formula (CH2O)n, where n can be 3-7.
 Hexose sugars have six carbon atoms, so have the formula C6H12O6. Hexose sugars include glucose,
galactose and fructose. These are isomers, with the same chemical formula (C6H12O6), but different
structural formulae. In animals glucose is the main transport sugar in the blood, and its concentration in
the blood is carefully controlled.
 Pentose sugars have five carbon atoms, so have the formula C5H10O5. Pentose sugars include ribose
and deoxyribose (found in nucleic acids and ATP) and ribulose (which occurs in photosynthesis).
 Triose sugars have three carbon atoms, so have the formula C3H6O3. Triose sugars are found in
respiration and photosynthesis.
Structural formula for Ribose
(C5H10O5)
Structural formula for -Glucose
(C6H12O6)
You need to know
these formulae!
HGS Biology A-level notes
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AS Biology Unit 1
page 7
Disaccharides
Disaccharides are formed when two monosaccharides are joined together by a glycosidic bond (C–O–C).
The reaction involves the formation of a molecule of water (H2O):
O
HO
O
OH
HO
O
OH
O
H2O
O
HO
OH
glycosidic bond
This shows two glucose molecules joining together to form the disaccharide maltose. This kind of reaction,
where two molecules combine into one bigger molecule, is called a condensation reaction. The reverse
process, where a large molecule is broken into smaller ones by reacting with water, is called a hydrolysis
reaction.
In general:
 polymerisation reactions are condensations
 breakdown reactions are hydrolyses
There are three common disaccharides:
Maltose (or malt sugar) is glucose–glucose. It is formed on digestion
O
of starch by amylase, because this enzyme breaks starch down into
two-glucose units. Brewing beer starts with malt, which is a maltose
O
Glucose
Glucose
O
HO
OH
solution made from germinated barley.
Sucrose (or cane sugar) is glucose–fructose. It is common in plants
O
because it is less reactive than glucose, and it is their main transport
sugar. It is the common table sugar that you put in your tea.
O
Glucose
O
HO
Fructose
O
Lactose (or milk sugar) is galactose–glucose. It is found only in
mammalian milk, and is the main source of energy for infant
O
HO
Galactose
O
Glucose
OH
mammals.
HGS Biology A-level notes
NCM/2/16
AS Biology Unit 1
page 8
Polysaccharides
Polysaccharides are chains of many glucose monomers (often 1000s) joined together by glycosidic bonds.
The main polysaccharides are starch, glycogen and cellulose.
1. Starch is the plant storage polysaccharide. It is insoluble and forms starch granules inside many plant
cells. Being insoluble means starch does not change the water potential of cells, so does not cause the
cells to take up water by osmosis. It is not a pure substance, but is a mixture of amylose and
amylopectin. Amylose is poly-(1-4) glucose, so is a long glucose chain that coils up into a helix held
together by hydrogen bonds.
Amylopectin is poly 1-4 glucose with some 1-6 branches. This gives it a more open molecular structure
than amylose. Because it has more ends, it can be broken more quickly than amylose by amylase
enzymes. Both amylose and amylopectin are broken down by the enzyme amylase into maltose, though
at different rates.
2. Glycogen is the animal storage polysaccharide and is
found mainly in muscle and liver cells. It is similar in
structure to amylopectin but with more branches, and
is sometimes called animal starch. Glycogen is broken
down
to
glucose
by
the
enzyme
glycogen
phosphorylase, and since there are so many ends, many
enzyme molecules can work simultaneously, so
glycogen can be broken down very quickly.
HGS Biology A-level notes
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AS Biology Unit 1
page 9
3. Cellulose is only found in plants, where it is the main component of cell walls. It is poly (1-4) glucose,
but with a different isomer of glucose. Starch and glycogen contain -glucose, while cellulose contains glucose, with a different position of the hydroxyl group on carbon 1. This means that in a cellulose chain
alternate glucose molecules are inverted.
This apparently tiny difference makes a huge difference in structure and properties. The  bond is
flexible so starch molecules can coil up, but the  bond is rigid, so cellulose molecules form straight
chains. Hundreds of these chains are linked together by hydrogen bonds between the chains to form
cellulose microfibrils. These microfibrils are very strong and rigid, and give strength to plant cells, and
therefore to young plants and also to materials such as paper, cotton and sellotape.
The -glycosidic bond cannot be broken by amylase, but requires a specific cellulase enzyme. The only
organisms that possess a cellulase enzyme are bacteria, so herbivorous animals, like cows and termites
whose diet is mainly cellulose, have mutualistic bacteria in their guts so that they can digest cellulose.
Carnivores and omnivores cannot digest cellulose, and in humans it is referred to as fibre.
Starch and Glycogen
Cellulose
 glycosidic bonds
 glycosidic bonds
flexible chains
straight chains
H bonds within each chain, forming helix
H bonds between chains, forming microfibrils
Can form H-bonds with water, so can be soluble
Can't form H bonds with water, so insoluble
Reacts with iodine to form blue-black complex
Doesn't react with iodine
Easy to digest
Difficult to digest
Storage role
Structural role
HGS Biology A-level notes
NCM/2/16
AS Biology Unit 1
page 10
Lipids
Lipids are a mixed group of hydrophobic compounds composed of the elements carbon, hydrogen, oxygen
and sometime phosphorus (CHOP). The most common lipids are triglycerides and phospholipids.
Triglycerides
Triglycerides, or triacylglycerols, are commonly known as fats or oils. They are made of glycerol and fatty
acids.
Glycerol is a small, 3-carbon molecule with
three alcohol (OH) groups.
Fatty acids are long molecules made of a nonpolar hydrocarbon chain with a polar carboxyl
acid group at one end. The hydrocarbon chain
can be from 14 to 22 CH2 units long. Because
the length of the hydrocarbon chain can vary it
is sometimes called an R group, so the formula
of a fatty acid can be written as R-COOH.
One molecule of glycerol joins together with three fatty acid molecules by ester bonds to form a
triglyceride molecule, in another condensation polymerisation reaction:
HGS Biology A-level notes
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page 11
Triglycerides are found in fatty (or adipose) tissue. They are used for:
 Energy storage. Triglyceride respiration yields more energy per unit mass than other compounds, so
adipose tissue is used a long-term energy store. However, triglycerides can't be mobilised quickly since
they are so insoluble, so are no good for quick energy requirements. Tissues that need energy quickly
(like muscles) instead use glycogen.
 Insulation. Adipose tissue under the skin (sub-cutaneous) is used to insulate warm-blooded mammals
against heat loss e.g. blubber in whales. A fatty myelin sheath electrically insulates nerve cells so the
electrical impulses travel faster.
 Waterproofing. Mammals’ fur and birds’ feathers contain the lipid lanolin for waterproofing. Insect
exoskeletons contain waxy lipids to stop water loss, and plants have a lipid waxy cuticle to reduce water
loss.
Saturated and Unsaturated Fats
 If the fatty acid chains in a triglyceride have no C=C double bonds, then they
are called saturated fatty acids (i.e. saturated with hydrogen). Triglycerides
with saturated fatty acids have a high melting point and tend to be found in
warm-blooded animals. At room temperature they are solids (fats), e.g. butter,
H
H
H
H
C
C
C
C
H
H
H
H
saturated
lard.
 If the fatty acid chains in a triglyceride do have C=C double bonds they are
called unsaturated fatty acids (i.e. unsaturated with hydrogen). Fatty acids with
H
H
H
H
Triglycerides with unsaturated fatty acids have a low melting point and tend to
C
C
C
C
be found in cold-blooded animals and plants. At room temperature they are
H
more than one double bond are called poly-unsaturated fatty acids (PUFAs).
liquids (oils), e.g. fish oil, vegetable oils. An “omega number” is sometimes used
H
unsaturated
to denote the position of a double bond, e.g. omega-3 fatty acids.
HGS Biology A-level notes
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page 12
Phospholipids
Lipids have a very low density, so the body fat of water mammals helps them to float easily Phospholipids
have a similar structure to triglycerides, but with a phosphate group in place of one fatty acid chain. There
may also be other groups attached to the phosphate. Phospholipids have a polar hydrophilic "head" (the
negatively-charged phosphate group) and two non-polar hydrophobic "tails" (the fatty acid chains).
or
This mixture of properties is fundamental to biology, for
phospholipids are the main components of cell membranes. When
mixed with water, phospholipids form droplet spheres with a
double-layered phospholipid bilayer. The hydrophilic heads facing
the water and the hydrophobic tails facing each other. This traps a
compartment of water in the middle separated from the external
water by the hydrophobic sphere. This naturally-occurring
structure is called a liposome, and is similar to a membrane
surrounding a cell (see pxx).
HGS Biology A-level notes
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page 13
Proteins
Proteins are the most complex and most diverse group of biological compounds. They have an astonishing
range of different functions, as this list shows.
structure
e.g. collagen (bone, cartilage, tendon), keratin (hair), actin (muscle)
enzymes
e.g. amylase, pepsin, catalase, etc (>10,000 others)
transport
e.g. haemoglobin (oxygen), transferrin (iron)
pumps
e.g. Na+K+ pump in cell membranes
motors
e.g. myosin (muscle), kinesin (cilia)
hormones
e.g. insulin, glucagon
receptors
e.g. rhodopsin (light receptor in retina)
antibodies
e.g. immunoglobulins
storage
e.g. albumins in eggs and blood, caesin in milk
blood clotting
e.g. thrombin, fibrin
lubrication
e.g. glycoproteins in synovial fluid
toxins
e.g. cholera toxin
antifreeze
e.g. glycoproteins in arctic flea
and many more!
Amino Acids
Proteins are made of amino acids. Amino acids
are made of the five elements C H O N S.
Amino acids are so-called because they
contain both an amino group and an acid
group. The general structure of an amino acid
molecule is shown on the right. There is a
central carbon atom (called the "alpha carbon",
C), with four different chemical groups
attached to it:
1. a hydrogen atom
2. a basic amino group (NH2 or NH+3 )
3. an acidic carboxyl group (COOH or
COO )
4. a variable "R" group (or side chain)
HGS Biology A-level notes
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There are 20 different R groups, and so 20 different amino acids. Since each R group is slightly different,
each amino acid has different properties, and this in turn means that proteins can have a wide range of
properties. The table on the next page shows the 20 different R groups, grouped by property, which gives
an idea of the range of properties. You do not need to learn these, but it is interesting to see the different
structures, and you should be familiar with the amino acid names. You may already have heard of some,
such as the food additive monosodium glutamate, which is simply the sodium salt of the amino acid
glutamate. There are 3-letter and 1-letter abbreviations for each amino acid.
Polypeptides
Amino acids are joined together by peptide bonds. The reaction involves the formation of a molecule of
water in another condensation polymerisation reaction:
When two amino acids join together a dipeptide is formed. Three amino acids form a tripeptide. Many
amino acids form a polypeptide. e.g.:
In a polypeptide there is always one end with a free amino (NH2) group, called the N-terminus, and one
end with a free carboxyl (COOH) group, called the C-terminus.
In a protein the polypeptide chain may be many hundreds of amino acids long. Amino acid polymerisation
to form polypeptides is part of protein synthesis. It takes place in ribosomes, and is special because it
requires an RNA template. The sequence of amino acids in a polypeptide chain is determined by the
sequence of the bases in DNA. Protein synthesis is studied in detail on pxx.
HGS Biology A-level notes
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page 15
The Twenty Amino Acid R-Groups
Simple R groups
Basic R groups
Glycine
Gly G
Lysine
Lys K
Alanine
Ala A
Arginine
Arg R
Valine
Val V
Histidine
His H
Leucine
Leu L
Asparagine
Asn N
Isoleucine
Ile I
Glutamine
Gln Q
Hydroxyl R groups
Acidic R groups
Serine
Ser S
Aspartate
Asp D
Threonine
Thr T
Glutamate
Glu E
Sulphur R groups
Ringed R groups
Cysteine
Cys C
Phenylalanine
Phe F
Methionine
Met M
Tyrosine
Tyr Y
Cyclic R group
Proline
Pro P
HGS Biology A-level notes
Tryptophan
Trp W
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page 16
Protein Structure
Polypeptides are just strings of amino acids, but they fold up and combine to form the complex and welldefined three-dimensional structure of working proteins. To help to understand protein structure, it is
broken down into four levels:
1. Primary Structure
This is just the sequence of amino acids in the polypeptide chain, so is not really a structure at all.
However, the primary structure does determine the rest of the protein structure. Most polypeptide chains
contain hundreds or even thousands of amino acids.
2. Secondary Structure
This is the most basic level of protein folding, and consists of a few basic motifs
that are found in almost all proteins. The secondary structure is held together by
hydrogen bonds between the carboxyl groups and the amino groups in the
polypeptide backbone. The two most common secondary structure motifs are
the -helix and the -sheet.
The -helix. The polypeptide chain is wound
round to form a helix. It is held together by
hydrogen bonds running parallel with the long
helical axis. There are so many hydrogen bonds
that this is a very stable and strong structure. Do
not confuse the -helix of proteins with the
famous double helix of DNA – helices are common
structures throughout biology.
The -sheet. The polypeptide chain zig-zags back
and forward forming a sheet of antiparallel strands.
Once again it is held together by hydrogen bonds.
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3. Tertiary Structure
This is the complete structure formed by the folding up of a
polypeptide chain. Every protein has a unique tertiary structure,
which is responsible for its properties and function. For example
the shape of the active site in an enzyme is due to its tertiary
structure. The tertiary structure is held together by bonds
between the R groups of the amino acids in the protein, and so
depends on what the sequence of amino acids is. These bonds
include:

hydrogen bonds, which are weak but numerous.

Ionic bonds (or salt bridges) between oppositely-charged R-groups e.g. NH+3 in lysine or arginine with
COO in aspartate or glutamate. These ionic bonds are stronger than hydrogen bonds but weaker
than covalent bonds.

covalent S–S bonds called sulphur bridges or disulphide bonds between two cysteine amino acids,
which are much strong and not easily broken.
4. Quaternary Structure
Almost all working proteins are actually composed of more than one polypeptide chain, and the quaternary
-S
Haemoglobin consists of four chains arranged in a
tetrahedral (pyramid) structure.
-S-S-
-S
-
structure is the arrangement of the different chains. There are a huge variety of quaternary structures e.g.:
Antibodies comprise four chains
arranged in a Y-shape.
Collagen consists of three chains in
a triple helix structure.
The enzyme ATP synthase is composed of
22 chains forming a rotating motor.
Actin consists of hundreds of globular chains
arranged in a long double helix.
These four structures are not real stages in the formation of a protein, but are simply a convenient
classification that scientists invented to help them to understand proteins. In fact proteins fold into all these
structures at the same time, as they are synthesised.
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The final three-dimensional shape of a protein can be classified as globular or fibrous.
Globular Proteins e.g. Haemoglobin
The vast majority of proteins are globular, i.e. they have a compact, roughly spherical structure. This group
includes enzymes, membrane proteins, receptors, transport proteins and storage proteins.
Haemoglobin is a globular protein found in red blood cells. One
molecule is composed of four globular polypeptide chains called
globins. There are two  chains with 141 amino acids each and
two  chains with 146 amino acids each, giving a total of 574
amino acids. Each chain contains a small non-polypeptide group
called haem, which has an iron atom at its centre. The haem
groups are attached to the globin polypeptide chains by covalent
sulphur bridges.
One oxygen molecule (O2) can bind to each iron atom, so a
haemoglobin molecule can bind up to four O2 molecules. The
polypeptide chains provide a suitable environment for the oxygen
molecules to bind reversibly, so haemoglobin acts as an effective
oxygen transport protein.
Fibrous Proteins e.g. Collagen
Fibrous proteins are long and thin, like ropes. They tend to have structural roles, such as collagen (bone),
keratin (hair), tubulin (cytoskeleton), actin (muscle), fibrin (blood clots) and fibroin (silk). They are always
composed of many polypeptide chains.
Collagen is a fibrous protein found in bone and
cartilage. A single molecule consists of three
long polypeptide chains linked by numerous
hydrogen bonds and wrapped round each other
in a triple helix. Each polypeptide chain is about
10,000 amino acids long and contains a simple
repeating sequence of just three amino acids (e.g. Gly-Pro-Ala). Many collagen molecules bind together to
form a fibril, and many of these fibrils link to form fibres. These collagen fibres have high tensile strength
and give strength and flexibility to cartilage, ligaments, tendons, bone, skin and blood vessels. Collagen is
also found in the cornea and lens of the eye. The synthesis of collagen requires vitamin C, which is why
vitamin C deficiency causes the disease scurvy, where connective tissue breaks down.
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Protein Denaturing
Since the secondary, tertiary and quaternary structures are largely held together by hydrogen bonds, the
three-dimensional structure of proteins is lost if the hydrogen bonds break. The polypeptide chain just folds
up into a random coil and the protein loses its function. This is called denaturing, and happens at
temperatures above about 50°C or at very low or high pH. Covalent bonds are not broken under these
conditions, so the primary structure is maintained (as are sulphur bridges).
Egg albumin denaturing
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Enzymes
Enzymes are biological catalysts. There are about 40,000 different enzymes in living cells, each controlling a
different chemical reaction. They increase the rate of reactions by a factor of between 106 to 1012 times,
allowing the chemical reactions that make life possible to take place at normal temperatures. They were
discovered in fermenting yeast in 1900 by Buchner, and the name enzyme means "in yeast".
 Intracellular enzymes catalyse all the metabolic reactions inside cells and organelles (such as
respiration, photosynthesis, DNA replication and protein synthesis). They also act as motors, membrane
pumps and receptors.
 Extracellular enzymes are synthesised inside cells but then exported out of cells and catalyse
reactions outside the cell. Digestive enzymes are the best example: they are synthesised in the cells of
the pancreas, but are secreted out of these cells and work in the lumen of the intestine.
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How do enzymes work?
There are three ways of thinking about enzyme catalysis. They all describe the same process, though in
different ways, and you should know about each of them.
1. Enzymes Manipulate the Substrate in the Active Site
Enzymes are proteins, and their function is determined by their complex 3-dimentional structure. The
reaction takes place in a small part of the enzyme called the active site, while the rest of the protein acts as
"scaffolding". The substrate molecule binds to the active site and the product is released.
active site
substrate
R-groups of amino
acids at the active site
substrate
protein
chain
Lysozyme – whole molecule
Close-up of substrate binding to
amino acids in the active site
The shape of an enzyme’s active site is complementary to the shape of the substrate molecule. This is called
the lock and key model, since the active site is like a lock and the substrate is like a key fitting into the lock.
The shape and properties of the active site are given by the amino acids around it, which form weak
hydrogen and ionic bonds with the substrate molecule. This means the active site can bind one substrate
only, in other words the enzyme is specific for that one reaction. Molecules with a different shape won’t fit
the active site, just as the wrong key won’t fit into a lock.
The lock and key analogy is quite good, but it doesn’t reflect the fact that enzyme molecules are flexible
(unlike locks). When a substrate binds, the whole enzyme changes shape, distorting the substrate molecule
in the active site and turning it into the product. For example if a bond in the substrate is to be broken,
that bond might be stretched by the enzyme, making it more likely to break. Alternatively if a bond is to be
made between two molecules, the two molecules can be held in exactly the right position and orientation
and “pushed” together, making the bond more likely to form. The enzyme can also make the local
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conditions inside the active site quite different from those outside (such as pH, water concentration,
charge), so that the reaction is more likely to happen. This flexible model is called the induced fit model,
and is a more accurate description of enzyme action.
Many enzymes also have small non-protein molecules called coenzymes at their active sites to help bind to
the substrate. Many of these are derived from dietary vitamins, which is why vitamins are so important.
2. Enzymes Take an Alternative Reaction Pathway
In any chemical reaction, a substrate (S) is converted into a product (P):
S
P
(There may be more than one substrate and more than one product, but that doesn't matter here.) In an
enzyme-catalysed reaction, the substrate first binds to the active site of the enzyme to form an enzymesubstrate (ES) complex, then the substrate is converted into product while attached to the enzyme, and
finally the product is released. This mechanism can be shown as:
E+S
ES
EP
E+P
The enzyme is then free to start again. The end result is the same (S
so that the S
P), but a different route is taken,
P reaction as such never takes place. In by-passing this step, and splitting the reaction up
into many small steps rather than one big step, the reaction can be made to happen much more quickly.
3. Enzymes Lower the Activation Energy
The way enzymes work can also be shown by considering
the energy changes that take place during a chemical
reaction. We shall consider a reaction where the product
has a lower energy than the substrate, so the substrate
naturally turns into product (in other words the
equilibrium lies in the direction of the product). Before it
can change into product, the substrate must overcome an
"energy barrier" called the activation energy (EA). The
larger the activation energy, the slower the reaction will
be because only a few substrate molecules will by chance have sufficient energy to overcome the activation
energy barrier. Imagine pushing boulders over a hump before they can roll downhill, and you have the idea.
Most physiological reactions have large activation energies, so they simply don't happen on a useful time
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scale. Enzymes dramatically reduce the activation energy of a reaction, so that most molecules can easily
get over the activation energy barrier and quickly turn into product.
For example for the breakdown of hydrogen peroxide (2H2O2
2H2O + O2):
 EA = 86 kJ mol-1 with no catalyst
 EA = 62 kJ mol-1 with an inorganic catalyst of iron filings
 EA = 1 kJ mol-1 in the presence of the enzyme peroxidase (catalase).
Active sites and binding sites
Enzymes and receptors are both protein molecules that work in similar ways. They have specific threedimensional shapes with a site where another molecule can bind.
Enzymes have an active site. The molecule that Receptors have a binding site. The molecule that
binds (the substrate) is changed and released as a binds (the ligand) is released unchanged.
different molecule (the product).
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Measuring the Rate of Enzyme Reactions
1. Firstly you need a signal to measure that shows the progress of the
reaction. The signal should change with either substrate or product
concentration, and it should preferably be something that can be
measured continuously. Typical signals include colour changes, pH
changes, mass changes, gas production, volume changes or turbidity
changes. If the reaction has none of these properties, it can sometimes
be linked to a second reaction that does generate one of these changes.
2. If you mix the substrate with enzyme and measure the signal, you will
obtain a time-course. If the signal is proportional to substrate
concentration it will start high and decrease, while if the signal is
proportional to product it will start low and increase. In both cases the
time-course will be curved (actually an exponential curve).
3. How do you obtain a rate from this time-course? One thing that is not
a good idea is to measure the time taken for the reaction, for as the
time-course shows it is very difficult to say when the reaction actually
ends: it just gradually approaches the end-point. The rate is in fact the
slope (or gradient) of the time-course, so we can see that the rate (and
slope) decreases as the reaction proceeds. The best measurement is
the initial rate - that is the initial slope of the time-course. This also
means you don't need to record the whole time-course, but simply take
one measurement a short time after mixing.
4. Repeat this initial rate measurement under different conditions (such as
different temperatures or substrate concentrations) and then plot a
graph of rate vs. the factor. Each point on this second graph is taken
from a separate initial rate measurement (or better still is an average of
several initial rate measurements under the same conditions). Draw a
smooth curve through the points.
Be careful not to confuse the two kinds of graph (the time-course and rate graphs) when interpreting data.
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Factors that Affect the Rate of Enzyme Reactions
1. Temperature
All chemical reactions get faster as the temperature increases, but with
enzyme reactions this is only true up to a certain temperature, above which
the rate slows down again. This optimum temperature is about 40°C for
mammalian enzymes but there are enzymes that work best at very different
temperatures, e.g. enzymes from the arctic snow flea work at -10°C, and
enzymes from thermophilic bacteria work at 90°C.
Up to the optimum temperature the rate increases geometrically with temperature (i.e. it's a curve, not a
straight line). The rate increases because the enzyme and substrate molecules both have more kinetic
energy so collide more often, and also because more molecules have sufficient energy to overcome the
(greatly reduced) activation energy. The rate is not zero at 0°C, so enzymes still work in the fridge (and
food still goes off), but they work slowly. Enzymes can even work in ice, though the rate is extremely slow
due to the very slow diffusion of enzyme and substrate molecules through the ice lattice.
This increase in rate with temperature would continue indefinitely except that the enzyme molecule itself is
affected by temperature. Above about 40°C there is enough thermal energy to break the weak hydrogen
bonds holding the secondary, tertiary and quaternary structures of the enzyme together, so the enzyme
(and especially the active site) loses its specific shape to become a random coil. The substrate can no longer
bind, and the reaction is no longer catalysed. This denaturation is usually irreversible. The optimum
temperature of enzymes is normally about 40°C because that is the temperature at which hydrogen bonds
break. This is also the reason why mammals and birds maintain their body temperature at around 40°C.
Remember that only the weak hydrogen bonds not peptide bonds are broken at these mild temperatures;
to break strong covalent bonds you need to boil in concentrated acid for many hours.
2. pH
Enzymes have an optimum pH at which they work fastest. For most enzymes
this is about pH 7-8 (physiological pH of most cells), but a few enzymes can
work at extreme pH, such as protease enzymes in animal stomachs, which
have an optimum of pH 1. The pH affects the charge of the R-groups of the
amino acids at the active site. For example carboxyl R-groups are uncharged
(COOH) in acid pH but negatively charged (COO–) in alkali pH. Similarly

amino R-groups are positively charged ( NH3 ) in acidic pH but uncharged (NH2) in alkali pH. These changes
can affect the shape as well as the charge of the active site, so the substrate can no longer bind and the
reaction isn't catalysed.
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3. Enzyme concentration
As the enzyme concentration increases the rate of the reaction increases
linearly, because there are more enzyme molecules available to catalyse the
reaction. At very high enzyme concentration the substrate concentration
may become rate-limiting, so the rate stops increasing. Normally enzymes
are present in cells in rather low concentrations.
4. Substrate concentration
The rate of an enzyme-catalysed reaction shows a curved dependence on
substrate concentration. As the substrate concentration increases, the rate
increases because more substrate molecules can collide with enzyme
molecules, so more reactions will take place. At higher concentrations the
enzyme active sites become saturated with substrate, so there are few free
enzyme molecules, so adding more substrate doesn't make much difference
(though it will increase the rate of E–S collisions).
5. Inhibitors
Inhibitors inhibit the activity of enzymes, reducing the rate of their reactions.
They are found naturally but are also used artificially as drugs, pesticides and
research tools. Inhibitors that bind fairly weakly and can be washed out are
called reversible inhibitors, while those that bind tightly and cannot be washed
out are called irreversible inhibitors.
There are two kinds of reversible inhibitors:
 Competitive Inhibitors are molecules with a
similar
structure
to
the
normal
substrate
molecule, and can fit into the active site of the
enzyme. They therefore compete with the
substrate for the active site, so the reaction is
slower. However, if the substrate concentration
is increased high enough the substrate will outcompete the inhibitor and the rate can approach a normal rate. The sulphonamide anti-bacterial drugs
are competitive inhibitors.
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 Non-competitive Inhibitors are molecules with a
quite different in structure from the substrate
molecule and do not fit into the active site.
They bind to another part of the enzyme
molecule, changing the shape of the whole
enzyme, including the active site, so that it can
no longer bind substrate molecules. Noncompetitive inhibitors therefore simply reduce
the amount of active enzyme (just like decreasing the enzyme concentration). Poisons like cyanide,
heavy metal ions and some insecticides are all non-competitive inhibitors.
The two types of inhibitor can be distinguished experimentally by carrying out a substrate vs. rate
experiment in the presence and absence of the inhibitor. If the inhibition is reduced at high substrate
concentration then the inhibitor is a competitive one.
Metabolic Pathways and End-Product Inhibition
The thousands of reactions taking place in every cell are arranged into metabolic pathways. The product of
one reaction is the substrate for the next, and each step has its own enzyme. These pathways only operate
when needed, so are under tight control. One form of control is end-product inhibition. If the final product
builds up then it inhibits the first enzyme in the pathways (usually by non-competitive inhibition), thus
reducing its own production. If the concentration of the final product is too low, then the first enzyme is no
longer inhibited, the pathway runs again, and more end product is made. This is a form of negative
feedback.
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DNA
DNA and its close relative RNA are perhaps the most important molecules in biology. They contain the
instructions that make every single living organism on the planet, and yet it is only in the past 50 years that
we have begun to understand them. DNA stands for deoxyribonucleic acid and RNA for ribonucleic acid,
and they are called nucleic acids because they are weak acids, first found in the nuclei of cells. They are
polymers, composed of monomers called nucleotides.
Nucleotides
Nucleotides contain the elements CHONP, and have three parts to them:
or more simply:
2-
 A phosphate group (PO4 ), which is negatively charged, and gives nucleic acids their acidic properties.
 A pentose sugar. If carbon 2' has a hydroxyl group attached then the sugar is ribose, found in RNA. If
the carbon 2' just has a hydrogen atom attached instead (as shown), then the sugar is deoxyribose,
found in DNA.
 A nitrogenous base. These are small organic basic groups that contain the elements CHON, so are
often called nitrogenous bases. There are four different bases in DNA nucleotides, called Adenine (A),
Cytosine (C), Guanine (G) and Thymine (T). RNA nucleotides also have four bases, but instead of
thymine RNA has Uracil (U). Adenine and Guanine have a double ring and are called purines while
thymine and cytosine have single rings and are called pyrimidines (you don't need to know their
structures).
The diagrams show that each base can form hydrogen bonds with another specific base to form
complementary base pairs. The pairs are always formed between a purine and pyrimidine (A with T and G
with C), so the two base pairs are the same size. Since there are four bases, there are four different
nucleotides:
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Base: Adenine (A)
Nucleotide: Adenosine
Cytosine (C)
Cytidine
Guanine (G)
Guanosine
Thymine (T)
Thymidine
The nucleotide above is shown with a single phosphate group, but in fact nucleotides can have one, two or
three phosphate groups. So for instance you can have adenosine monophosphate (AMP), adenosine
diphosphate (ADP) and adenosine triphosphate (ATP). These nucleotides are very common in cells and
have many roles other than just part of DNA. For example, ATP is used as the energy transfer molecule,
while AMP and GTP are used as messenger chemicals.
Nucleotide Polymerisation
Nucleotides polymerise by forming phosphodiester bonds between
carbon 3' of the sugar and an oxygen atom of the phosphate. This is
a condensation reaction. The bases do not take part in the
polymerisation, so there is a sugar-phosphate backbone with the
bases extending off it. This means that the nucleotides can join
together in any order along the chain. Two nucleotides form a
dinucleotide, three form
a trinucleotide, a few form an
oligonucleotide, and many form a polynucleotide.
A polynucleotide has a free phosphate group at one end, called the
5' end because the phosphate is attached to carbon 5' of the sugar,
and a free OH group at the other end, called the 3' end because it's
on carbon 3' of the sugar. The terms 3' and 5' are often used to
denote the different ends of a DNA molecule.
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Structure of DNA
The three-dimensional structure of DNA was discovered in 1953 by Watson and Crick in Cambridge, using
experimental data of Wilkins and Franklin in London, for which work they won a Nobel Prize. The main
features of the structure are:
 DNA is double-stranded, so there are two polynucleotide stands alongside each other. The strands are
antiparallel, i.e. they run in opposite directions.
 The two strands are wound round each other to form a double helix (not a spiral, despite what some
textbooks say).
 The two strands are joined together by hydrogen bonds between the bases. The bases therefore form
base pairs, which are like rungs of a ladder.
 The base pairs are specific. A only binds to T (and T with A), and C only binds to G (and G with C).
These are called complementary base pairs. This means that whatever the sequence of bases along one
strand, the sequence of bases on the other stand must be complementary to it. (Incidentally,
complementary, which means matching, is different from complimentary, which means being nice.)
 DNA is a very long molecule, so it can store a great deal of information (a single human DNA molecule
has 3 billion base-pairs and is 1m long). The bases are protected on the inside of the molecule and the
billions of hydrogen bonds holding the two strands together make DNA a very stable molecule.
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Function of DNA
DNA contains genes, and genes control characteristics. A gene is simply a section of DNA around 10002000 base pairs long. The four bases can appear in any order along the DNA molecule, so their sequence
can encode information, like writing with a 4-letter alphabet. DNA doesn’t control characteristics directly,
but instead DNA controls characteristics by controlling protein synthesis. In fact a gene is defined as a
sequence of bases on a DNA molecule coding for a sequence of amino acids in a polypeptide chain.
It is the numerous proteins in a cell (mostly enzymes) that control what the cell does, and therefore the
characteristics of the organism.
So there is an old and a new definition of a gene that say the same thing:
A gene is an inherited factor that
controls a particular characteristic.
A gene is a section of DNA that
codes for a particular polypeptide.
This process of making proteins and so controlling characteristics is called gene expression (because the
gene "expresses" itself). Expression is split into two steps: transcription and translation, so this diagram
summarises what DNA does:
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The Genome
No one knows exactly how many genes we humans have to control all our characteristics, but the current
best estimate is around 20 thousand. The sum of all the genes in an organism is called the genome, and this
table shows the estimated number of genes in different organisms:
Species
phage 
Eschericia coli
Saccharomyces cerevisiae
Caenorhabditis elegans
Drosophila melaogaster
Homo sapiens
Common name
virus
bacterium
Yeast
nematode worm
fruit fly
human
length of DNA (kbp)*
48
4 639
13 500
90 000
165 000
3 150 000
no of genes
60
4 000
6 000
~10 000
~10 000
~20 000
*kbp = kilo base pairs, i.e. thousands of nucleotide monomers.
Coding and Non-Coding DNA
Surprisingly, a lot of the DNA in eukaryotes does not code for polypeptides. In fact, only about 2% of the
DNA in a eukaryotic cell is coding DNA. The rest, called non-coding DNA, does not form genes. There are
two kinds of non-coding DNA:
 Non-coding regions of DNA within a gene are called introns (for interruption sequences), while the
coding parts of DNA are called exons (for expressed sequences). All eukaryotic genes have introns, and
they are usually longer than the exons, so genes are often much longer than they really need to be!
 Non-coding regions of DNA between genes are called satellite DNA. Satellite DNA often contains
simple base sequences repeated many times (sometime thousands of times).
Non-coding DNA was originally termed junk DNA, but in fact it probably serves many different functions.
 Some non-coding DNA is structural, helping to coil the DNA molecule into chromosomes.
 Some non-coding DNA has a control function, regulating when genes are expressed.
 Some non-coding DNA is involved in DNA replication.
 Some non-coding DNA contains unused copies of genes (pseudogenes).
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Replication of DNA
DNA is copied, or replicated, before every cell division, so that one identical copy can go to each daughter
cell. The method of DNA replication is obvious from its structure: the double helix unzips and two new
strands are built up by complementary base-pairing onto the two old strands.
1. Replication starts at a specific sequence on the DNA molecule called the replication origin.
2. The enzyme DNA helicase unwinds and separates the two strands of DNA, breaking the hydrogen
bonds between the base pairs.
3. The new DNA is built up from the four nucleotides (A, C, G and T) that are present in the nucleoplasm.
These nucleotides attach themselves to the bases on the old strands by complementary base pairing.
Where there is a T base, only an A nucleotide will bind, and so on.
4. The enzyme DNA polymerase joins the new nucleotides to each other by strong covalent
phosphodiester bonds, forming the sugar-phosphate backbone. This enzyme is enormously complex and
contains 18 subunits.
5. The two strands wind up to form a double helix.
6. The two new DNA molecules are identical to the old molecule. Each new DNA molecule contains one
"new" strand and one "old" strand.
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Replication Forks and DNA Ligase
In eukaryotes replication is speeded up by taking place at many sites along the DNA simultaneously. These
multiple sites of replication lead to structures called replication forks:
As the polymerase enzymes from two forks meet, there is always a gap, or nick, formed, where adjacent
nucleotides cannot be joined by polymerase. These nicks are joined (or ligated) by another enzyme, DNA
ligase, which makes phosphodiester bonds to complete the sugar-phosphate backbone of the new stands.
Occasionally, mistakes are made in replication, i.e. an incorrect base pair (such as A-C) is formed. To allow
for this, there are repair enzymes that follow DNA polymerase along the new DNA molecules, checking
for mismatched base pairs. Incorrect nucleotides are cut out and replaced, and repaired with DNA ligase
again.
Even with multiple origins, DNA replication can take a few hours, and in fact this limits the speed of cell
division. One reason bacteria can reproduce so fast is that they have a relatively small amount of DNA.
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The Meselson-Stahl Experiment
This replication mechanism is called semi-conservative replication, because each new DNA molecule contains
one new strand and one old strand. This need not be the case, and alternative theories included conservative
replication and dispersive replication:
The evidence for the semi-conservative method came from an elegant experiment performed in 1958 by
Matthew Meselson and Franklin Stahl. They used the bacterium E. coli together with the technique of
density gradient centrifugation, which separates molecules on the basis of their density.
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RNA
As we have seen, gene expression is split into two parts – transcription and translation, and it involves the
other nucleic acid – RNA (ribonucleic acid). RNA is a nucleic acid like DNA, but with 4 differences:
 RNA is made of ribose nucleotides instead of deoxyribose nucleotides
 RNA has the base uracil instead of thymine (so the four bases in RNA are A, U, C and G).
 RNA is single stranded (though it can fold into 3-dimentional structures)
 RNA is shorter than DNA
There are three kinds of RNA, with three different jobs:
Messenger RNA (mRNA)
mRNA carries the "message" that codes for a particular protein from the nucleus (where the DNA master
copy is) to the cytoplasm (where proteins are synthesised). It is single stranded and just long enough to
contain one gene only (about 1000 nucleotides). It has a short lifetime and is degraded soon after it is used.
Transfer RNA (tRNA)
tRNA is an “adapter” that matches amino acids to their codon. tRNA is
only about 80 nucleotides long, and it folds up by complementary base
pairing to form a looped clover-leaf structure. At one end of the
molecule there is always the base sequence ACC, where the amino acid
binds. On the middle loop there is a triplet nucleotide sequence called
the anticodon. There are 64 different tRNA molecules, each with a
different anticodon sequence complementary to the 64 different codons.
The amino acids are attached to their tRNA molecule by specific
aminoacyl tRNA synthase enzymes. These are highly specific, so that each
amino acid is attached to a tRNA adapter with the appropriate anticodon.
Ribosomal RNA (rRNA)
rRNA together with proteins forms ribosomes, which are the site of
mRNA translation and protein synthesis. Ribosomes have two subunits,
small and large, and are assembled in the nucleolus of the nucleus and
exported into the cytoplasm. rRNA is coded for by numerous genes in
many different chromosomes. Ribosomes free in the cytoplasm make
proteins for use in the cell, while those attached to the RER make proteins for export.
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Transcription - RNA Synthesis
DNA never leaves the nucleus, but proteins are synthesised in the cytoplasm, so a copy of each gene is
made to carry the “message” from the nucleus to the cytoplasm. This copy is mRNA, and the process of
copying is called transcription. As we have seen, eukaryotic genes contain non-coding sequences (introns)
as well as the coding sequences (exons). The introns need to be removed before the mRNA can be
translated into protein. This removal is called post-transcriptional modification.
1. The start of each gene on DNA is marked by a special sequence of bases called the promoter. The
enzyme RNA polymerase binds to the DNA molecule at the promoter. RNA polymerase is a huge
protein complex, consisting of 17 separate proteins, each with many polypeptide chains. One of these
proteins is a helicase enzyme, and it unwinds the DNA double helix, but only for a short length (about
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17 base pairs) within the RNA polymerase enzyme. Thus unwound region of DNA is called the
transcription bubble.
2. Only one of these separated DNA strands is used to make RNA. This strand is called the template
strand (see box below for more on terminology) because it acts as the template on which the new RNA
molecule is built. The other DNA strand is not used in transcription. The new RNA molecule is built up
from the four ribose nucleotides (A, C, G and U) in the nucleoplasm. The ribose nucleotides bind to the
bases on the DNA template strand by complementary base pairing, just as in DNA replication.
The mRNA strand will be used to code for a polypeptide, so the sequence of bases on mRNA is called
the sense sequence. The template DNA strand that is copied is therefore called the DNA antisense
strand, since its sequence is complementary to the RNA sequence. The other DNA strand is not used in
transcription. Since its sequence is complementary to the antisense DNA strand (and the same as the
RNA strand), it is called the DNA sense strand.
3. The new RNA nucleotides are joined to each other by strong covalent phosphodiester bonds by
another part of the RNA polymerase enzyme.
4. The RNA polymerase enzyme moves along the DNA molecules, unwinding the DNA in front and
rewinding it behind. Only about 8 RNA base pairs remain attached to the DNA template strand inside
the RNA polymerase active site, since the mRNA molecule peels off from the DNA as it is made. When
the polymerase enzyme reaches the end of the gene transcription stops, and the RNA polymerase
enzyme detached from the DNA. The mRNA molecule is thus just the length of the gene.
5. The initial mRNA that is transcribed is called the primary transcript or pre-mRNA. Pre-mRNA is an
exact copy of the gene on the DNA, so it contains exons and introns.
6. The introns in the mRNA are cut out and the exons are joined together by enzymes in a process called
splicing. Some of this splicing is done by the RNA intron itself, acting as an RNA enzyme. The recent
discovery of these RNA enzymes, or ribozymes, illustrates what a diverse and important molecule RNA
is. Other splicing is performed by RNA/protein complexes called snurps.
7. The result is a shorter mature RNA containing only exons. The introns are broken down. This
completes the post-transcriptional modification.
8. The mRNA diffuses out of the nucleus through a nuclear pore into the cytoplasm. There, it attaches to
ribosomes for translation. It usually doesn't have far to go to find a ribosome, as many are attached to
the rough endoplasmic reticulum, which is contiguous with the nuclear envelope.
Prokaryotic DNA does not have introns, so mRNA processing is not needed.
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The Genetic Code
There are 20 different amino acids and only 4 different bases, so the bases are read in groups of three. This
gives 43 or 64 combinations, more than enough to code for 20 amino acids. A triplet of three bases coding
for an amino acid is called a codon, and the meaning of each of the 64 codons is called the genetic code.
Because there are more codons than amino acids, most amino acids are coded for by more than codon.
For example CCA, CCT, CCC and CCG all code for the amino acid glycine. Some codons also mark the
beginning and end of a gene.
There are several interesting points from this code:
 The code is degenerate, i.e. there is often more than one codon for an amino acid.
 The degeneracy is on the third base of the codon, which is therefore less important than the others.
 One codon means "start" i.e. the start of the gene sequence. It is AUG, which also codes for
methionine. Thus all proteins start with methionine (although it may be removed later). AUG in the
middle of a gene simply codes for methionine.
 Three codons mean "stop" i.e. the end of the gene sequence. They do not code for amino acids.
 The code is non-overlapping, e.g. the sequence UGCACG contains only the two codons UGC and
ACG. It can never be read as the overlapping codons UGC, GCA, CAC, ACG.
The process of synthesising proteins is called translation because it translates from the 4-base DNA
language to the 20-amino acid protein language.
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Translation - Protein Synthesis
1. A ribosome attaches to the mRNA at an initiation
codon (AUG). The ribosome encloses two codons.
2. The first tRNA molecule with an amino acid attached
(met-tRNA) diffuses to the ribosome. Its anticodon
attaches to the first mRNA codon by complementary
base pairing.
3. The next amino acid-tRNA attaches to the adjacent
mRNA
codon
(CUG,
leu
in
this
case)
by
complementary base pairing.
4. The bond between the amino acid and the tRNA is
cut and a peptide bond is formed between the two
amino acids. These operations are catalysed by
enzymes in the ribosome called ribozymes.
5. The ribosome moves along one codon so that a new
amino acid-tRNA can attach. The free tRNA molecule
leaves to collect another amino acid. The cycle
repeats from step 3.
6. The polypeptide chain elongates one amino acid at a
time, and peels away from the ribosome, folding up
into a protein as it goes. This continues for hundreds
of amino acids until a stop codon is reached, when
the ribosome falls apart, releasing the finished protein.
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A single piece of mRNA can be translated by many ribosomes simultaneously, so many protein molecules
can be made from one mRNA molecule. A group of ribosomes all attached to one piece of mRNA is called
a polyribosome, or a polysome.
Post-Translational Modification
In eukaryotes, proteins often need to be altered before they become fully functional. Because this happens
after translation, it is called post-translational modification. Modifications are carried out by other enzymes
and include: chain cutting, adding methyl or phosphate groups to amino acids, adding sugars (to make
glycoproteins) or lipids (to make lipoproteins).
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Mutations
A mutation is a change in DNA in a cell (a change in genotype). A mutation may have no effect on
phenotype or it may may a major effect, including death. There are many different types of mutation,
categorised into two groups: gene mutations and chromosome mutations:
Here, we shall look at gene mutations. On p xx we shall look at chromosome mutations.
Gene Mutations
Gene mutations are changes in DNA base sequences due to a base-pairing error during DNA replication.
DNA is a very stable molecule, and it doesn't suddenly change without reason, but bases can change when
DNA is being replicated. Normally replication is extremely accurate, and there are even error-checking
procedures in place to ensure accuracy, but very occasionally mistakes do occur (such as a T–C base pair).
There are three kinds of gene mutation, shown in this diagram:
 Substitution mutations only affect one amino acid, so tend to have less severe effects. In fact if the
substitution is on the third base of a codon it may have no effect at all, because the third base often
doesn't affect the amino acid coded for (e.g. all codons beginning with CC code for proline). These are
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called silent mutations. However, if a mutation leads to a premature stop codon the protein will be
incomplete and certainly non-functional. This is called a nonsense mutation.
 Deletion and insertion mutations have more serious effects because they are frame shift mutations i.e.
they change the codon reading frame even though they don't change the actual sequence of bases. So all
amino acids "downstream" of the mutation are wrong, and the protein is completely wrong and nonfunctional. However, the effect of a deletion can be cancelled out by a near-by insertion, or by two
more deletions, because these will restore the reading frame. A similar argument holds for a
substitution.
Impact on phenotype
Most mutations have no phenotypic effect because they occur in non-coding DNA, or the mutated gene is
not expressed in this cell, or the mutation doesn't change the amino acid. These are called silent mutations,
and we all have a few of these.
Mutations in coding DNA that is expressed will change the cell’s phenotype like this:
change in
base of
DNA
different
codon
different
amino acid
in protein
change in
primary structure
of protein
change in secondary
and tertiary
structure of protein
change in
protein
function
change in
cell
function
Many of the proteins in cells are enzymes, and most changes in enzymes will stop them working (because
there are far more ways of making an inactive enzyme than there are of making a working one). When an
enzyme stops working a reaction in a cell doesn't happen, so the cell's function is changed. It's just possible
(though unlikely) that a mutation could make a modified enzyme that actually worked faster than the
original enzyme. This means cell's function could be improved.
Since mutations change genes, they give rise to new alleles (i.e. different versions of genes). A cell with the
original, functional gene has one allele, while a cell with a mutated, non-functional version of the same gene
has a different allele. For example in a flower a “red” allele might encode a functional enzyme that makes a
red pigment, while a “white” allele might encode a non-functional enzyme so the flower stays white.
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Sickle Cell Anaemia
We saw on px that haemoglobin is a protein made of four polypeptide chains. A single-base mutation in the
gene for one of these chains causes the disease sickle cell anaemia.
Normal haemoglobin
DNA
ACT CCT
mRNA
UGA GGA
Polyeptide Thr Pro
GAG
CUC
Glu
GAG
CUC
Glu
TCT
AGA
Ser
Sickle cell haemoglobin
DNA
ACT CCT GTG
mRNA
UGA GGA CAC
Polyeptide Thr Pro Val
GAG
CUC
Glu
TCT
AGA
Ser
Although this substitution mutation only changes a single amino acid, this slightly changes the shape of the
whole haemoglobin molecule, and causes them to link together to form long chains, distorting the red
blood cells into sickle shapes. These sickled red blood cells are less flexible than normal cells, so can block
capillaries and arterioles, causing cell death and sever pain. Sickle cells are also destroyed by the spleen
faster than they can be made, so not enough oxygen can be carried in the blood (anaemia). Without
treatment this phenotype is fatal in early childhood, though modern medical intervention can extend life
expectancy to 50.
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Viruses
Viruses are the smallest and most abundant of biological entities. They are considered biological since they
contain DNA, can reproduce themselves and evolve by natural selection. However they are not considered
to be living organisms because they are not made of cells, and cannot do any of the basic characteristics of
life on their own. They are in fact more like complex chemicals than simple living organisms. Viruses are
obligate parasites that can only reproduce inside host cells, which get damaged in the process, leading to
disease. Viruses are thought to have arisen from lengths of DNA that became separated from their cells.
Virus Structure
Viruses have very simple structures, usually made of just three components:
 Nucleic acid, which can be DNA or RNA, and single or double-stranded. The nucleic acid typically codes
for 5-100 proteins (by comparison, the bacterium E. coli has about 4000 genes).
 A protein coat called a capsid, made of subunits called capsomeres.
Because capsids are composed of many repeating subunits, they tend
to have simple geometrical shapes, such as a helix or icosahedron
(20 triangular faces).
 A lipid envelope, not made by the virus itself, but derived from a host cell membrane. The lipid envelope
may contain matrix proteins, which attach the capsid to the envelope, and glycoproteins, which attach
the virus to host cells.
A complete virus particle outside a cell is called a virion. Virions are too small to see with a light
microscope and were first seen in the 1930s using the electron microscope. Once inside a host cell virions
dismantle into their separate parts so that the virus can be reproduced.
We need to know about four viruses:
Structure
Nucleic
Acid
Phage 
complex
ss DNA
TMV
Helix
ss RNA
(+ve sense)
6.4 kb
3 genes
Tobacco
plants
Ebola virus
Enveloped
helix
ss RNA
(-ve sense)
19 kb
7 genes
Human cells
HIV
enveloped
icosahedron
ss RNA
(retrovirus)
10 kb
9 genes
Human
white blood
cells
HGS Biology A-level notes
Genome
size
6.4 kb
70 genes
Host cell
E.coli
bacteria
Disease
-
Life Cycle
lysogenic
and lytic
Tobacco
mosaic
disease
haemorrhagic
budding
fever
AIDS
latency and
budding
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Phage Lambda
Bacterial viruses are known as bacteriophages (or just
phages), which means “bacteria eaters”. Phage Lambda ()
infects the bacterium E.coli, which lives in human intestines.
Like many phages, it has a complex structure combining
icosahedral and helical capsids. The head contains doublestranded DNA.
Tobacco Mosaic Virus (TMV)
Tobacco mosaic virus (TMV) was the first virus to
be discovered, and is one of the smallest. It infects a
wide variety plants (not just tobacco), where it
causes a mosaic-like mottling of the leaves and causes a great deal of economic damage. TMV has a very
simple structure: just a coil of single-stranded RNA surrounded by a helical capsid made of 2130
capsomeres.
Ebola Virus
Ebola virus is a human virus that
causes severe and often fatal
hemorrhagic fever. It is a very
large
virion
negative-sense
consisting
of
a
single-stranded
RNA surrounded by a large flexible helical capsid 80nm in dameter, which in turn is enclosed in a lipid
envelope. The virus usually coils up.
Human Immunodeficiency Virus (HIV)
The human immunodeficiency virus (HIV) infects
the white blood cells of the immune system and
causes the fatal disease AIDS. HIV comprises
single-stranded RNA, surrounded by an icosahedral
capsid, which is in turn surrounded by a sphere of
proteins attached to a lipid envelope.
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Viral Nucleic Acid and Reproductive Cycles
Unlike cells (which all contain double-stranded DNA), viruses contain a variety of nucleic acid types,
including single and double-stranded DNA and RNA. Indeed viruses are usually classified by their type of
nucleic acid, as this table shows:
Classification
Nucleic Acid Type
Group
examples
1
Double-stranded DNA
Phage , smallpox, chicken pox, herpes
2
Single-stranded DNA
Parvo-virus
3
Double-stranded RNA
Reovirus
4
Single-stranded RNA (positive sense)
TMV, rhinovirus (common cold), polio
5
Single-stranded RNA (negative sense)
Ebola, measles, mumps, flu
6
Single-stranded RNA (retrovirus)
HIV, leukemia
70% of viruses contain RNA, but there are different ways in which this RNA is used to make proteins:

Positive sense RNA has a sequence that can be translated directly by ribosomes to synthesise proteins,
in other words the viral RNA is mRNA.

Negative sense RNA has the complementary sequence to mRNA, so a complementary mRNA copy
has to be made using a special RNA-directed RNA polymerase enzyme.

Retroviruses have a unique enzyme called reverse transcriptase, which makes double-stranded DNA
from a single-stranded RNA template (in other words the reverse of transcription). This DNA can
then be integrated into the host cell’s genomic DNA.
Viruses can only reproduce inside a host cell. They rely on the host cell’s ribosomes, enzymes, nucleotides,
amino acids, tRNA and even ATP to synthesise new viral nucleic acids and proteins. Different viruses have
different reproductive cycles depending on their type of nucleic acid. The different ways these nucleic acids
are used in the reproductive cycles of the four viruses are shown below.
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Phage  Reproductive Cycle
When phage infects bacterial cells the capsid remains outside the cell and injects the DNA into the
cytoplasm. There are now two different pathways the virus can follow.

In the lysogenic pathway the phage DNA is integrated into the bacterium’s own DNA, where it
becomes a latent prophage. In this latent state the virus doesn’t cause any harm, but is replicated along
with the bacterial DNA every time the cell divides. At some environmental signal the phage switches
to the lytic pathway.

In the lytic pathway the prophage separates from the bacterial DNA. The viral DNA is replicated,
transcribed and translated using bacterial enzymes and ribosomes, to make thousands of phage capsid
proteins. These proteins spontaneously assemble to form hundreds of capsids, which burst out of the
cell. This causes cell lysis and kills the cell.
TMV Reproductive Cycle
TMV first infects plants through damage to cell walls and the capsid is disassembled. Because the viral
single-stranded RNA is positive sense it can be used directly as mRNA. This mRNA is translated into capsid
proteins using plant cell ribosomes and amino acids, and the RNA is also replicated. The virus re-assembles
and spreads throughout the plant through plasmodesmata between cells, and through the phloem vessels.
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Ebola Reproductive Cycle
The Ebola virus can infect a variety of human cells, including blood and liver cells. The viral envelope fuses
with the cell membrane releasing the capsid into the cytoplasm. The negative-sense single-stranded RNA
molecule is used a template for the viral enzyme RNA-directed RNA polymerase to make many copies of
mRNA. This mRNA is translated using human cell ribosomes and amino acids to make viral capsid proteins.
The virus reassembles and exits the cell through budding, collecting the envelope of host cell membrane as
it leaves. This kills the cell.
HIV Reproductive Cycle
HIV infects T-lymphocyte cells of the human immune system. Virus particles cells attach to the lymphocyte
membrane then the envelope and cell membrane fuse together, releasing the capsid inside the cytoplasm.
HIV is a retrovirus, which means it can copy its RNA to make double-stranded DNA using the viral enzyme
reverse transcriptase. This DNA is integrated into the nuclear DNA, where it can remain latent for many
years. This is another example of latency, like phage . At an environmental trigger the viral DNA is
suddenly expressed, making viral proteins, which assemble to form virions, which exit the cell by budding,
killing the cell.
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Viral Diseases
All viruses cause disease. Since viruses cannot reproduce on their own, they must infect host cells to
reproduce, and this reproduction inevitably harms or kills the host cell. Viruses cause disease in plants,
animals, fungi and even bacteria. Human viral diseases are common and vary from harmless to fatal.
Disease
common cold
influenza
measles
Rubella (German measles)
Mumps
Chickenpox
hepatitis B
Ebola haemorrhagic fever
AIDS
Virus
Rhinovirus
Myovirus
Paramyxovirus
Rubella virus
paramyxovirus
Varicella zoster virus
DNA virus
Ebola virus
HIV
Treating Viral Diseases
It is easy to kill organisms (for example using heat, acid, high-energy radiation or bleach), but none of these
methods can be used once a pathogen has already infected a human host. Treating diseases requires
selectively toxic drugs that kill the pathogen but not the host cells. For example bacteria can be killed using
antibiotics that target enzymes only found in bacteria, and fungi can be killed using antifungals that target
enzymes only found in fungi.
But viruses are not living cells and do not carry out metabolic reactions, which could be targeted by
antiviral drugs. The only time viruses are active is when they reproduce inside a host cell. Viral
reproduction mostly uses host cell enzymes, but there are a few viral enzymes that can be targeted. For
example (you don’t need to learn these, just appreciate them):

AZT and acyclovir are nucleotide analogues that competitively inhibit the enzyme reverse transcriptase,
only found in retroviruses like HIV. These drugs are therefore effective treatments for AIDS.

Raltegravir inhibits the viral enzyme integrase, used by retroviruses to integrate their DNA into the
host DNA.

Tamiflu inhibits the viral enzyme neuraminidase that many viruses use to escape from cells. So this drug
stops the viruses reproducing and spreading.

Ribavirin is a RNA nucleotide analogue that inserts itself into viral RNA, thus stopping its translation.
Even with these drugs, some viral diseases are almost impossible to treat and rely on combination therapy,
where three of four different antiviral drugs are used together. These have shown great success, especially
with AIDS, but are very expensive and so not available in many countries.
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Viral Disease Epidemics
An epidemic is when an infectious disease spreads far more quickly and widely than usual. If an epidemic
spreads across continents it is called a pandemic. So the most important strategy for controlling epidemics
is to limit the spread of the disease, especially for viral diseases, which are so difficult to treat with drugs. A
good example is the 2014 outbreak of Ebola disease in Guinea, West Africa. The outbreak quickly spread
to neighbouring countries Sierra Leone, Liberia, Mali and Sengal. The epidemic remains today (2015), with a
total of 11,000 deaths so far.
There are three stages in preventing the spread of an epidemic:
1. Identify the outbreak and respond quickly. In the 2013 Philippines Earthquake the health implications
were immediately obvious and emergency health teams were on site within days. But in the 2014 West
Africa Ebola outbreak, it took six months before the World Health Organisation (WHO) coordinated
an international response.
2. Reduce spread by enforcing simple cultural and hygiene practices, such as hand-washing by healthcare
workers and others in contact with patients and victims. About two-thirds of the Ebola cases in Guinea
were caused by the local practice washing dead bodies, which are still infectious. This practice has now
been stopped.
3. Impose travel restrictions to and from the region to prevent the infection from spreading to other
countries. Many countries (including the USA) still impose restrictions on those travelling to and from
the Ebola region. On 8 August 2014 a cordon sanitaire (or quarantine area) was established round a
triangular area between Guinea, Liberia, and Sierra Leone, where 70% of the known cases had been
found. No one was allowed to enter or leave, in order to stop further spread. The quarantine has since
been reduced to a series of checkpoints for hand-washing and measuring body temperature on major
roads throughout the region.
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BLANK PAGE
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DNA and Chromosomes
The DNA molecule in a single human cell is 1 m long, so is 10 000 times longer than the cell in which it
resides (< 100m). (Since an adult human has about 1014 cells, all the DNA is one human would stretch
about 1014 m, which is a thousand times the distance between the Earth and the Sun.) In order to fit into
the cell nucleus the DNA in eukaryotes is cut into shorter lengths and each length is tightly wrapped up
with histone proteins to form a complex called chromatin.
Just before cell division the DNA is replicated, and more histone proteins are synthesised, so there is
temporarily twice the normal amount of chromatin. Following replication the chromatin then coils up even
tighter to form short fat bundles called chromosomes. These are about 100 000 times shorter than fully
stretched DNA, and therefore 100 000 times thicker, so are thick enough to be seen with the light
microscope. Each chromosome is roughly X-shaped because it contains two replicated copies of the DNA.
The two arms of the X are therefore identical. They are called chromatids, and are joined at the
centromere. (Do not confuse the two chromatids with the two strands of DNA.) The complex folding of
DNA into chromosomes is shown below.
one chromatid
centromere
micrograph of a single chromosome
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Karyotypes and Homologous Chromosomes
If a dividing cell is stained with a special fluorescent
dye and examined under a microscope during cell
division,
the
individual
chromosomes
can
be
distinguished. They can then be photographed and
studied. This is a difficult and skilled procedure, and
it often helps if the chromosomes are cut out and
arranged in order of size.
This display is called a karyotype, and it shows
several features:
 Different species have different number of chromosomes, but all members of the same species have the
same number. Humans have 46 (this was not known until 1956), chickens have 78, goldfish have 94, fruit
flies have 8, potatoes have 48, onions have 16, and so on. The number of chromosomes does not appear
to be related to the number of genes or amount of DNA.
 Each chromosome has a characteristic size, shape and banding pattern, which allows it to be identified
and numbered. This is always the same within a species. The chromosomes are numbered from largest
to smallest.
 Chromosomes come in pairs, with the same size, shape and banding pattern, called homologous pairs
("same shaped"). So there are two chromosome number 1s, two chromosome number 2s, etc., and
humans really have 23 pairs of chromosomes.
 One pair of chromosomes is different in males and females. These are called the sex chromosomes, and
are non-homologous in one of the sexes. In humans the sex chromosomes are homologous in females
(XX) and non-homologous in males (XY). In other species it is the other way round. The non-sex
chromosomes are called autosomes, so humans have 22 pairs of autosomes, and 1 pair of sex
chromosomes.
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It is important to understand exactly what homologous chromosomes are. We have two copies of each
chromosome because we inherit one copy from each parent, so each homologous pair consists of a
maternal and paternal version of the same chromosome. Since the homologous chromosomes contain the
same genes, this also means we have two copies of each gene (again, one from each parent). This is why we
write two letters for each gene in a genetic cross. The two homologous chromosomes may have the same
versions (or alleles) of the gene (e.g. AA), or they may have different alleles, because one copy is a mutation
(Aa).
Sometimes the chromosomes in a cell nucleus are represented by rods
called ideograms, although these structures never actually exist because
the chromatin is usually uncoiled. Each ideogram represents the long
coiled DNA molecule in one chromosome. This diagram shows a pair of
homologous chromosomes with two genes marked. The plant cell
containing these chromosomes is homozygous for the seed shape gene
(RR) and heterozygous for the flower colour gene (Pp).
The only time chromosomes can actually be seen is during cell division.
At this point in the cell cycle each chromosome is made of two identical
chromatids, because each DNA molecule has now been replicated. This
diagram shows the same pair of homologous chromosomes during
mitosis. The two chromatids in each chromosome contain the same
alleles because they're exact replicas of each other. But again the two
homologous chromosomes contain the same genes but different alleles.
Chromatin
DNA + histone complex during interphase
Chromosome
compact X-shaped form of chromatin formed (and visible) during mitosis
Chromatids
the two arms of an X-shaped chromosome. The two chromatids are identical since they
are formed by DNA replication.
Homologous
chromosomes
two chromosome of the same size and shape, one originating from each parent. They
contain the same genes, but different alleles.
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The Cell Cycle
Cell theory states that new cells are always formed by division of old cells. The normal form of cell division
in eukaryotes is mitosis, which forms two genetically-identical “daughter cells”. Mitosis is used to make new
cells for:
 Growth, when an organism is growing in mass. A human’s 1014 body cells are made by mitosis from
one original zygote.
 Replacement, to replace cells that are lost. Each day humans replace 107 gut epithelial cells in faeces;
107 skin epidermal cells in house dust; and 1011 red blood cells that are recycled.
 Repair, to replace cells that are damaged by injury e.g. to mend a broken bone or a cut in the skin.
 Asexual Reproduction, in some eukaryotic organisms (e.g. yeast, strawberry, starfish).
 Artificial Cloning of plants and animals in biotechnology.
The life of a cell from one division to the next is called the cell cycle, and has three main phases:
1. Interphase. This is when the cell grows and carries out its normal functions (e.g. respires, synthesises
molecules, secretes hormones, contracts, transmits nerve impulses, etc.). Typically 90% of the cell cycle
is spent in interphase. Interphase is sub-divided into:
 Gap phase G1, where the cell grows back to its original size. Genes are expressed into whatever
proteins are needed by this cell, and organelles are replicated.
 Synthesis phase S, where DNA and histones are replicated in preparation for mitosis. This can
take a few hours.
 Gap phase G2, where spindle proteins are synthesised, ready for mitosis.
2. Mitosis Phase. This is where the nucleus divides to make two nuclei with identical copies of the DNA
that was replicated during the previous S phase. The mitotic phase can be sub-divided into the four
phases: prophase, metaphase, anaphase and telophase (details below).
3. Cytokinesis. This is division of the cytoplasm, to form two daughter cells with one nucleus each.
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Control of the Cell Cycle
The cell cycle is tightly controlled, so that cells only divide when they need to. The cycle is controlled
through checkpoints, where the cell is checked to see if it is safe to proceed to the next phase of the cycle:

The G1 checkpoint is only passed if there is a need for more cells, signalled by an external factor such
as a growth hormone. This prevents excessive cell division.

The G2 checkpoint is only passed if DNA has been replicated correctly and there is the correct number
of chromosomes.

The M checkpoint is only passed if mitosis has been completed successfully and there are two identical
nuclei.
Sometimes the checkpoints can fail, perhaps due to mutations or viral infection. This failure allows cells to
divide uncontrollably, leading to cancer.
The cell cycle can last from hours to years in different cell types. For example embryonic cells divide very
quickly with no growth in between, so they have very short interphases, just long enough to replicate the
DNA. Skin cells divide about every 12 hours on average, liver cells every 2 years, and muscle cells never
divide at all after maturing, so remain in the G1 phase for decades.
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Mitosis
The point of mitosis is to make two genetically-identical cells, and the purpose of the stages shown below is
to ensure that the two daughter cells each receive a replicated copy of the DNA. This cell has just two
pairs of homologous chromosomes.
Interphase
 no chromosomes visible
 DNA, histones and centrioles all replicated
Prophase
 chromosomes condensed and visible
 centrioles at opposite poles of cell
 nucleolus disappears
Metaphase
 nuclear envelope disappears
 chromosomes align along equator of cell
 spindle fibres (microtubules) connect centrioles to
chromosomes
Anaphase
 centromeres split, allowing chromatids to separate
 microtubules shorten, pulling chromatids towards poles,
centromeres first
 Other microtubules lengthen, pushing poles apart and
elongating cell.
Telophase
 spindle fibres disperse
 nuclear envelopes form
 chromatids uncoil and become too thin to see
Cytokinesis
 In animal cells a ring of actin filaments forms round the
equator of the cell, and then tightens to form a cleavage
furrow, which splits the cell in two.
 In plant cells vesicles move to the equator, line up and
fuse to form two membranes called the cell plate. A new
cell wall is laid down between the membranes, which
fuses with the existing cell wall.
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Meiosis
Meiosis is the special cell division used by sexually-reproducing organisms to make gametes. It starts with
DNA replication, like mitosis, but then proceeds with two divisions one immediately after the other.
Meiosis therefore results in four daughter cells rather than the two cells formed by mitosis. Each division
has four phases, like mitosis, but, while meiosis II is just like mitosis, meiosis I has a number of significant
differences.
Division I
Division II
Interphase
Meiosis division II
proceeds immediately
without any interphase.
There is no replication of
DNA.
DNA, histones and
centrioles all replicated
Prophase I
Prophase II
Chromosomes condense
and homologous
chromosomes join
together to form
bivalents.
Metaphase I
Centrioles move to form
new poles at 90° to
original poles.
Chromosomes already
coiled.
Metaphase II
Bivalents line up on the
equator.
Anaphase I
Telophase I and
Cytokinesis I
HGS Biology A-level notes
Bivalents separate and
homologous
chromosomes move to
opposite poles.
Chromatids do not
separate.
Two nuclei with
replicated
chromosomes.
Chromosomes don’t
uncoil. Cells divide, but
remain joined.
Chromosomes line up on
new equator.
Anaphase II
Centromeres finally split,
separating chromosomes.
Chromatids move to
opposite poles.
Telophase II and
Cytokinesis II
Nuclear envelopes form
around each set of
chromosomes. Cells
divide, but remain joined
to form a tetrad.
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Meiosis differs from mitosis in two important aspects (apart from the two divisions):
Firstly, in meiosis the chromosome number is halved from the normal diploid number (2n) to the haploid
(half) number (n). This is necessary so that the chromosome number remains constant from generation to
generation, so in sexual reproduction meiosis is always followed by fertilisation at some point in the life
cycle:
The halving is done in a particular way: meiosis ensures that each haploid cell has one of each homologous
pair of chromosomes. So for example human gametes have 23 chromosomes: one of each homologous
pair. Remember that other species have different haploid numbers. The changes in chromosome number
can also be shown as a graph of DNA mass over time:
Secondly, in meiosis the chromosomes are re-arranged during meiosis to form new combinations of alleles.
This genetic recombination is vitally important and is a major source of genetic variation. It means for
example that of all the millions of sperm produced by a single human male, the probability is that no two
will be identical. The whole point of meiosis and sex is to introduce genetic variation, which allows species
to adapt to their environment and so to evolve. There are two sources of genetic variation in meiosis:
crossing over and Independent assortment. We’ll look at each of these in turn.
Crossing Over
This happens at prophase I of meiosis, when the bivalents first form. While the two homologous
chromosomes are joined in a bivalent, bits of one chromosome are swapped (crossed over) with the
corresponding bits of the other chromosome.
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The points at which the chromosomes actually cross over are called chiasmata (singular chiasma), and they
involve large, multi-enzyme complexes that cut and join the DNA. There is always at least one chiasma in a
bivalent, but there are usually many, and it is the chiasmata that actually hold the bivalent together. The
chiasmata can be seen under the microscope and they can give the bivalents some characteristic strange
shapes. There are always equal amounts crossed over, so the chromosomes stay the same length.
Crossing over means that maternal and paternal alleles can be combined even though they are on physically
different chromosomes. In the example in the diagram some gametes will have the new genotype
combinations Br or bR. This potentially allows any combination of alleles to form and, since there are
around 20 000 genes in humans, there is the potential to make an astronomically large number of
combinations.
Independent Assortment
This happens during metaphase I of meiosis, when the homologous chromosomes join together to form
bivalents that line up on the equator. Each bivalent is made up of two homologous chromosomes, which
originally came from two different parents (they’re often called maternal and paternal chromosomes). Since
they can line up in any orientation on the equator, the maternal and paternal versions of the different
chromosomes can be mixed up in the final gametes.
In this simple example with 2 homologous chromosomes (n=2) there are 4 possible different gametes (22).
In humans with n=23 there are over 8 million possible different gametes (223). This is just the number of
different arrangements of chromosomes. When we consider all the different rearrangements of genes
within chromosomes due to crossing over we can understand why every gamete produced by an individual
is genetically unique.
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Chromosome Mutations
Earlier we looked at gene mutations, where a few bases in DNA were changed, leading to new alleles. But
mutations can also happen on a larger scale, when large regions of a chromosome, perhaps containing
hundreds of genes, can be changed. These large mutations are known as chromosome mutations, and
include nondisjunction (which changes the number of chromosomes) and translocation, which changes the
structure of chromosomes.
Nondisjunction
Nondisjunction is a chromosome mutation that changes the number of chromosomes in a cell. It is caused
by a fault in anaphase of meiosis, where the chromatids or chromosomes normally separate and move to
opposite poles. In nondisjunction the chromatids or chromosomes remain stuck together and move
together to one pole of the cell. This nondisjunction can happen at anaphase I or anaphase II, but in both
cases it results in some gametes with an extra chromosome, and some with a missing chromosome.
If an abnormal gamete with too many or too few chromosomes is fertilised then the resulting zygote will
show polysomy in that chromosome, since there will be the wrong number of chromosomes. Some
examples of polysomy are shown below.
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Trisomy, e.g. Down syndrome
If a gamete with an extra chromosome is fertilised by a normal
gamete then the resulting zygote will show trisomy in that
chromosome, since there will be three copies instead of two.
Trisomy is almost always fatal early in embryo development, as
it appears that the extra chromosome presents too great a
burden on cell division and gene expression. The only common
exception
is
trisomy
in
the
smallest
chromosome
–
chromosome 21 (21 is actually smaller than 22). Trisomy 21
causes Down syndrome, which, although not fatal, does have severe consequences on the individual’s
phenotype. Symptoms of Down syndrome include particular recognisable facial features, short height, heart
defects, poor vision, severe learning difficulties and a shorter life expectancy.
Monosomy e.g. Turner syndrome
If a gamete with a missing chromosome is fertilised by a normal
gamete then the resulting zygote will show monosomy in that
chromosome, since there will only be one copy instead of two.
Monosomy is almost always fatal early in embryo development,
since a full complement of genes is required for normal cell
function. The only exception is the X chromosome: it is possible
to survive with a single copy of the X chromosome (after all that’s
all males have!). Monosomy X (often written as X0 in contrast to
the normal XX) is called Turner syndrome and occurs about once in every 5000 births. X0 individuals
appear female but their sex organs do not mature at adolescence, and they are sterile. They have normal
intelligence.
Polyploidy
Polyploidy happens when nondisjunction causes all the chromosomes to remain together, forming a gamete
with two copies of every chromosome (in other words a diploid gamete). If this gamete is fertilised by a
normal haploid gamete the resulting zygote has three copies of the entire genome – it’s triploid.
Surprisingly, polyploidy has little adverse effect on phenotype and is quite common in nature, especially in
the plant kingdom. For example apples are triploid (3n)
and wheat is hexaploid (6n). It seems that carrying an
extra set of chromosomes (polyploidy) is easier than
carrying just one extra chromosome (polysomy).
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Translocation
A translocation is a chromosome mutation where one part of a chromosome is swapped with a part of a
different chromosome. This sounds a bit like crossing over in meiosis, but in crossing over equal parts are
swapped between homologous chromosomes, whereas in translocation the swapped parts can be unequal
in size and they are swapped between non-homologous chromosomes. The result is different-sized
chromosomes, and indeed translocations can be identified by their appearance in a karyotype.
In this example the bottom part of a chromosome 1 (regions labelled C and D) is swapped with the bottom
part of a chromosome 2 (region labelled F). This is an unequal translocation, so chromosome 1 gets smaller
and chromosome 2 gets bigger. Exactly the same genes and alleles are still present, albeit some are now
located on different chromosomes. Translocation mutations are therefore usually harmless, with little or
no effect on the phenotype. Around 1 in 500 people are thought to contain translocation mutations. There
are however two possible complications caused by a translocation:
1. Cells with a translocation can undergo mitosis without any problems, but meiosis doesn’t work
properly since some of the homologous chromosomes are no longer truly homologous and so cannot
form bivalents properly. Even if meiosis completes, some of the gametes produced will be missing
certain regions of some chromosomes and therefore hundreds of genes (see diagram below). If these
gametes are fertilised the resulting embryo will be unviable. So individuals with a translocation may be
perfectly normal but are unsuccessful in having children, often resulting in miscarriage. This difficulty in
having children can be the first time someone may realise they have a translocation.
2. When the translocated regions are cut and swapped into their new locations they may disrupt a gene.
This disrupted gene will no longer function. Several different kinds of cancer have been found to be
caused by translocations in genes that regulate the cell cycle (pxx). Alternatively a new “fusion gene”
may be formed by joining two parts of genes together, which can cause unpredictable effects of the
phenotype.
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Sexual Reproduction
Sexual reproduction is the production of offspring from two parents using gametes. Sexual reproduction
involves two stages: meiosis, which halves the chromosome number, and fertilisation (the fusion of two
gametes to form a zygote), which restores it again.
Gametes
Gametes are the haploid sex cells that will fuse together to form a new diploid individual. In sexuallyreproducing species there are two kinds of gametes – male and female.
Male gametes
Female gametes
Male gametes are small cells that can move. If they Female gametes (ova or eggs in animals, ovules in
can propel themselves they are called motile (e.g. plants) are relatively large cells and they tend to be
animal sperm) but if they can easily be carried by the stationary. They contain food reserves (lipids,
wind or animals they are called mobile (e.g. plant proteins, carbohydrates) to nourish the embryo
pollen). They are produced in very large numbers. after fertilisation, and they are produced in fairly
Human males for example release about 108 sperm small numbers. Human females for example release
in one ejaculation.
Male: Many, Mobile, Minute
about 500 ova in a lifetime.
Female: Few, Fixed, Food
It is this difference in gametes that actually defines the sex of an individual. Those individuals that produce
small mobile gametes are the males, and those that produce the larger gametes are the females. In some
species (such as most flowering plants) the same individual organisms can produce both male and female
gametes, so they do not have distinct sexes and are called hermaphrodites. In other species (such as
mammals) there are two distinct sexes, each producing their own gametes. These organisms are called
unisexual.
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Sexual Reproduction in Humans
Gametogenesis in Humans
The male gametes, sperms, are made in the testes of males, while the female gametes, eggs or ova, are
made in the ovaries of females. The differences between male and female gametes are illustrated by these
diagrams of sperm and ova, and in the way the gametes are made (gametogenesis).
Spermatogenesis
Sperm are made continually in the testes of males after puberty. Undifferentiated primordial germ cells
multiply by mitosis to make vast numbers of diploid precursor cells called spermatogonia, which grow until
they are called primary spermatocytes. The spermatocytes divide by meiosis to make haploid spermatids,
which differentiate to become spermatozoa (or sperm), which are capable of fertilising an ovum.
Oogenesis
Oogenesis is very different from spermatogenesis in several respects.

The process starts in the ovaries of a female before she is born! While in the uterus, the primordial
germ cells of a female fetus start dividing to produce a large number of oogonia, which then grow so
that a girl is born with a few million diploid primary oocytes already formed in her ovaries. No more
primary oocytes will be formed after birth.

After puberty, one primary oocyte starts to develop each month of the menstrual cycle. The primary
oocyte starts meiosis, but stops after the first division. The division is unequal, with one daughter cell
retaining almost all the cell cytoplasm and organelles while the other cell is much smaller: just a
nucleus surrounded by a small amount of cytoplasm. The small cell is called a polar body and has no
further role, often dying. The large cell is the secondary oocyte and is released from the ovary at
ovulation to move down the oviduct. The advantage of this unequal division is that the ovum contains
the nutrients in the cytoplasm to nourish the embryo after fertilisation.

The second division of meiosis does not occur until after the ovum has been fertilised by a sperm.
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Fertilisation in Humans
In humans fertilisation takes place near the top of the oviduct. The sperm are carried there by currents in
the uterus and oviducts. They only use their tails to swim when close to the ovum. On the millions of
sperm released, on a few hundred reach the egg.
1. Numerous sperm use their flagella to swim through the follicle cells.
2. The sperm release hydrolytic enzymes from their acrosomes by exocytosis (the acrocsome reaction).
These enzymes partially digest the zona pellucida, allowing many sperm to swim through. The enzymes
from many sperm are required to digest a path through the zona pellucida.
3. When the first sperm reaches the ovum cell membrane the two membranes fuse and the sperm nucleus
enters the cytoplasm of the ovum. The rest of the sperm cell degrades, so the sperm only contributes a
nucleus to the zygote. All the cytoplasm and organelles (Including the DNA in the mitochondria) are
contributed by the ovum.
4. The entry of the sperm nucleus triggers a series of reactions in the ovum (called the cortical reaction).
Cortical granules fuse with the cell membrane releasing enzymes that cause the elements of the zona
pellucida to cross-link, preventing any other sperm from entering the ovum and preventing polyspermy.
5. The entry of the sperm nucleus also stimulates the egg nucleus to complete meiosis II, forming a third
polar body and the haploid female nucleus.
6. The male and the female nuclei do not fuse immediately, but instead the DNA in both nuclei replicate
and condense to form chromosomes. Then both nuclear envelopes disappear leaving the two sets of
chromosomes free in the cytoplasm. The cell is now a diploid cell – the zygote, which immediately starts
its first mitosis.
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Embryo Development in Humans
Day 0
Fertilisation takes place near the top of the oviduct and the zygote continues to move down
the oviduct driven by the beating of the cilia that line the oviduct.
Day 1-3
As it moves the zygote divides repeatedly by cleavage – a special form of mitosis with short
interphase and no growth phase. The total mass of the cells is the same as the original zygote
(which is why the ovum had to be such a large cell).
Day 4
After four days and three divisions the ball of 8 cells (the morula) arrives in the uterus.
Day 5
After seven divisions there are 128 cells, arranged in a hollow ball called a blastocyst.
Day 8-9
This blastocyst embeds itself in the lining of the uterus (the endometrium) in a process called
implantation. This is the start of pregnancy and the embryo can now receive nutrients from the
mother’s cells, allowing it to grow.
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Sexual Reproduction in Flowering Plants
Flowering plants all reproduce sexually and the flower itself is their reproductive organ. Almost all
flowering plants are bisexual or hermaphrodite, which means that each plant’s flower contains both male
and female sex organs (stamens and carpels respectively). This diagram shows the main parts of a flower:
Gemetogenesis in Plants
The male gametes (microspores) are produced in the anthers, while the female gametes (megaspores) are
produced in the ovule, inside the ovary.
Microsporogenesis
Inside most anthers are four pollen sacs containing thousands of diploid cells called microsporophytes or
pollen mother cells. These cells divide by meiosis to form four haploid microspores, each of which will
develop into a pollen grain. To form a pollen gradin the cell develops a tough thick wall (which helps in the
dispersal of the pollen grains to other plants), and the nucleus undergoes mitosis to form two haploid
nuclei called the generative nucleus and the pollen tube nucleus. The generative nucleus will divide once
more by mitosis to form two haploid male gametes, but only after the pollen grain lands on a female stigma
and germinates.
Megasporogenesis
Inside each ovule is a large diploid megasporocyte (or megaspore mother cell). The megasporocyte divides
by meiosis to form four haploid megaspores, but the division is uneven, with one megaspore containing
almost all the cytoplasm of the mother cell. The other three megaspores are very small and disintegrate.
The remaining megaspore grows until it fills most of the ovule and its nucleus divides three times by mitosis
to form eight haploid nuclei. Membranes then form splitting the embryo sac into seven cells: one female
gamete; two polar nuclei sharing the most of the cytoplasm and five smaller cells.
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Fertilisation in Plants
1. The pollen grains (immature male gametophytes) are released from the anthers and dispersed by wind
or insects to other flowers. If pollen lands on the stigma of a plant of the same species, that plant is
pollinated. Unlike animals, a plant can pollinate itself (self-pollination), but more usually the pollen lands
on a different plant of the same species (cross-pollination).
2. If the pollen grain has landed on a suitable stigma, it will germinate by absorbing water, swelling and
splitting open. The generative nucleus divides by mitosis to form two haploid male gamete nuclei. The
pollen grain is now a mature gametophyte.
3. A pollen tube emerges from the split and grows down the style towards the ovary. The pollen tube
secretes enzymes to digest the tissue in front of it, and uses these digested products to grow. The
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pollen tube nucleus stays at the tip of the pollen tube, controlling its growth. The two male gamete
nuclei also move down the tube behind the pollen tube nucleus.
4. When the pollen tube reaches the ovary it enters the female embryo sac through a small opening
called the micropyle. The pollen tube nucleus has now done its job and it degenerates, while the two
male gametes enter the embryo sac.
5. Both male gametes now fertilise female cells in the embryo sac in a process unique to sexual
reproduction in flowering plants called double fertilisation.

One male gamete fuses with the female gamete to form a diploid zygote.

The other male gamete fuses with the two polar nuclei to form a triploid primary endosperm cell.
6. Following fertilisation the flower develops into a seed that can survive in a dormant state and be
dispersed.

This zygote develops into the embryo. Once it has been dispersed it will germinate and form
shoots and roots and eventually grow into a new plant.

The primary endosperm grows and develops into a food store called the endosperm. This
nourishes the developing embryo. Coconut “meat” is an example of endosperm tissue.

The embryo sac develops into the seed surrounding the embryo and protecting it during
dispersal and germination.

The ovary wall develops into a fruit to aid seed dispersal. Different fruits have different
properties depending on their method of dispersal (e.g. wind, animals, self-dispersal, etc.)
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Classification
There are millions of different types of organisms on the planet, from large animals and plants to
microscopic fungi and bacteria, and each type is unique. We call each different type a species. Biologists
have so far found and named around 1.5 million different species, and the more we find, the more we
realise how few we know. Our knowledge is patchy: we know a lot about vertebrate animals and flowering
plants, but not much about bacteria, fungi or worms. We know a lot about species living in temperate
lands, but little about those living in the tropics, where 75% of all species probably live. The total number of
living species may well exceed 10 million, and if we include extinct species the figure is increased by a factor
of 100. A good classification system is needed to keep track of all this variety. The science of classification is
called taxonomy.
Principles of Taxonomy
Many different classification systems have been devised, but the one biologists use is based on the work of
the Swede Carolus Linnaeus in the mid-18th century. Linnaeus introduced three important innovations.
 He devised a hierarchical structure for classification. In a hierarchy organisms with similar characteristics
are grouped together, and these groups are contained within larger composite groups. There is no
overlap between groups, and a species can only appear once. These two diagrams represent hierarchical
classifications, and both forms are used in biology.
 He gave each rank in the hierarchy a standard name. There are seven ranks or levels in the biologists’
classification. The smallest group of similar organisms is the species; closely related species are grouped
into genera (singular genus), genera into families, families into orders, orders into classes, classes into
phyla (singular phylum), and phyla into kingdoms. (Some modern classifications also include a higher rank
called the domain, as we shall see.) So you need to remember KPCOFGS.
 He introduced the binomial nomenclature for naming organisms unambiguously. This simply consists of
the first two ranks: the generic name (with a capital letter) and the specific name (with a small letter),
and he used Latin names rather than different local-language names. The binomial names are italicised
when printed (or underlined if hand-written) and after a first mention, the generic name can be
abbreviated to the first letter e.g. Panthera leo (lion) and P.tigris (tiger). For most purposes the binomial
name is enough to identify an organism, but a full 7-rank lineage can be given to avoid confusion.
Linnaeus’s universal standard system replaced non-standard common and local names, and is still universally
used today.
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What is a Species?
For such a fundamental and apparently simple concept, the species is surprisingly hard to define. Here are
four different definitions, each focussing on a different aspect of the “species”:
1. Morphology definition: members of the same species have similar characteristics. These
characteristics could include their appearance or morphology (e.g. body shape, number of legs, leaf
shape, etc.); the structure of their cells (e.g. composition of organelles, ribosomes, cell walls); their
biochemistry (e.g. particular metabolic pathways or storage molecules). This is the most obvious
definition, and is still useful, but it can be misleading for determining a phylogeny. It can be difficult to
distinguish between homologous structures (which look similar because they arose from a common
ancestor, e.g. the pentadactyl limb); and analogous structures (which look similar because they arose
independently to do a similar job, e.g. a wing).
2. Ecological definition: members of the same species have the same ecological niche. An organism’s
niche is its role in the ecosystem: where it lives, how it feeds, what conditions it needs to survive, who
it competes with, how it reproduces and so on. Members of the same species must have the same
niche, since that is what they are adapted to.
3. Reproduction definition: members of the same species can breed together in their natural
environment to produce fertile offspring but cannot breed with members of other species. This is the
most useful definition, as it can be tested fairly easily, and applies directly to the process of speciation
itself (pxx), but it too has its limitations. For one thing, this definition doesn’t apply to the millions of
asexually-reproducing organisms, nor to extinct species. And even for sexually-reproducing species,
two organisms that would not normally breed in the wild (perhaps because they live in different parts
of the world) may be able to interbreed in the artificial environment of a zoo, a garden or a lab. This is
particularly true of plants, which can quite easily be hybridised. Even in the wild, some closely-related
species can interbreed, such as a horse and donkey to produce a mule, but the offspring is usually
sterile, so is not a viable organism.
4. Evolutionary definition: members of the same species share a unique common ancestor with each
other but not with members of any other species. This is the most modern definition of a species and
is the basis of phylogenetics – placing species in their correct evolutionary family tree. This can only be
done by analysing the DNA sequences of different species to see how closely related they really are
(see below). It can still be difficult to deduce relationships accurate since some genes can be
transferred from one species to another by horizontal gene transfer.
Biologists will no doubt continue to debate this species problem, but that doesn’t stop us using the species
as a useful basic unit of classification.
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Phylogenetics
We now know that all life on Earth is related, and all species have arisen through evolution. So the aim of
taxonomists today is to develop phylogenies; family trees representing true evolutionary relationships,
rather than just convenient groupings. Phylogenies are represented by
phylogenetic trees, just like family trees. Branching points on phylogenetic
trees can be interpreted in two ways:

Going forward in time from the past, they represent speciation events,
when one species splits in two (see pxx).

Going backward in time from the present, they represent the most
recent common ancestor (MRCA) of all the species after the branch.
In the tree shown here, species A and B are closely related because they have
a recent common ancestor (at point Y), while they are more distantly related
to species C because their common ancestor with C lived further in the past
(at point X).
This diagram (from the Ancestor’s Tale) shows the phylogeny of some mammals. The numbers are the
times in My between branches:
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Classification using DNA
Phylogenies and true ancestry can only be determined by comparing the DNA of different species, since it
is only DNA that is actually passed down from generation to generation. DNA can be extracted from the
cells of living organisms and the sequence of bases read using sequencing machines (see below). Over long
periods of evolutionary time, DNA slowly accumulates random mutations.
 Closely related living species have similar DNA sequences, because they have a recent common
ancestor and their DNA has only been separated for a short time.
 More distantly related species have more different sequences because their common ancestor lived
longer ago and their DNA has been separated for a longer time, so has had more time to accumulate
different mutations.
Because the DNA mutations happen at a steady and predictable rate, the number of differences in DNA
sequences can be used as a molecular clock to estimate the time since the last common ancestor. For
example, the gene for the protein cytochrome C mutates at an average rate of 1 base every 4 million years,
so if two living species have 3 bases differences in their genes for cytochrome C, they must have diverged
about 12 million years ago.
There are many millions of bases in the DNA of most organisms, so there is a vast amount of information
about the evolutionary history of each organism in its DNA. Various genes are used to compare species:
 The gene for the blood protein haemoglobin is found in all animals.
 The gene for the respiratory enzyme cytochrome C is found in all eukaryotes.
 The gene for the small subunit ribosomal RNA molecule (16S rRNA) is found in all known organisms.
This 16S rRNA molecule is ideal for classification studies because it and accumulates mutations only very
slowly (since its role is so fundamental), so its sequence can be compared over long periods of time. The
16S rRNA gene is only about 1500 bases long, small enough to be sequenced easily using early, crude
sequencing techniques but also large enough to retain organism-specific information.
HGS Biology A-level notes
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page 77
Sequencing DNA
How can we find the base sequence of a piece of DNA? DNA sequencing is based on a beautifully elegant
technique developed by Fred Sanger in Cambridge in 1975, and now called Sanger Sequencing.
1. Label 4 test tubes A, T, C and G. Into each test tube add: a
sample of the DNA to be sequenced (containing many millions
of individual molecules); a radioactive primer (so the DNA can
be visualised later on the gel); the four DNA nucleotides and
the enzyme DNA polymerase.
2. In each test tube add a small amount of a special modified
dideoxy nucleotide that cannot form a phosphodiester bond
and so stops further synthesis of DNA. Tube A has dideoxy A
(A*), tube T has dideoxy T (T*), tube C has dideoxy C (C*)
and tube G has dideoxy G (G*). The dideoxy nucleotides are
present at about 1% of the concentration of the normal
nucleotides.
3. Let the DNA polymerase synthesise many copies of the DNA
sample. From time to time at random a dideoxy nucleotide will
be added to the growing chain and synthesis of that chain will
then stop. A range of DNA molecules will be synthesised
ranging from full length to very short. The important point is
that in tube A, all the fragments will stop at an A nucleotide. In
tube T, all the fragments will stop at a T nucleotide, and so on.
4. The different fragment of DNA are now going to be separated
by electrophoresis – a form of chromatography used to
separate different pieces of DNA on the basis of their length.
To do this the DNA samples from the four tubes are placed
into four wells at one end of a thin slab of gel made of agarose,
and covered in a buffer solution.
5. An electric current is passed through the gel. Each nucleotide
in a molecule of DNA contains a negatively-charged phosphate
group, so DNA is attracted to the anode. As the molecules
move through the gel, and longer lengths of DNA are retarded
by the gel so move more slowly than shorter lengths. So the
shorter the DNA molecule, the further it will move in a given
time. At the end of the run the current is turned off.
HGS Biology A-level notes
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page 78
6. The DNA on the gel cannot be seen, so it must be visualised.
This can be done with a coloured stain, but this is not very
sensitive. Usually the DNA samples at the beginning are labelled
with a radioactive isotope such as 32P, then visualised using
autoradiography. Photographic film is placed on the finished gel
in the dark for a few hours, and the radiation from any
radioactive DNA on the gel exposes the film, so the position of
the DNA shows up as dark bands on the film. This method is
extremely sensitive.
7. The dark bands show the DNA fragments, sorted by length.
Since every fragment in present, from 1 nucleotide to the full
length, the sequence can simply be read off the gel starting with
the smallest fragment (just one nucleotide) at the bottom and
reading upwards.
Sequencing Whole Genomes
Sanger’s sequencing method was used to sequence the16,569 base pairs of human mitochondrial DNA and
the 48,502 base pairs of bacteriophage λ. His method was later modified and automated, so DNA
sequencing machines can now read gels automatically to give a digital sequence in a computer.
Such sequencing machines were used in the 13-year international project to sequence the complete
3-billion base sequence of the human genome, completed in 2003 at a cost of around $100 million.
Sequencing technology is improving all the time and modern machines can sequence one million bases per
second, equivalent to 1 human genome per hour! It is now possible for individuals to have their personal
genomes sequenced.
The entire genomes of thousands of species have now been
sequenced, allowing more detailed phylogenies to be deduced.
By 2016, the genomes of some 1,000 eukaryotic species and
10,000 prokaryotic species had been published. There are a
number of projects to organise the sequencing of different
groups of life on Earth. For example the Global Genome
Initiative aims to sequence at least one species from every one
of the 9,500 known animal and plant families.
HGS Biology A-level notes
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AS Biology Unit 1
page 79
Bioinformatics
These genome sequences represent vast amounts of data. Bioinformatics is a new branch of biology
concerned with storing, analysing, using and making sense of all that biological data. The sheer quantity of
data is so large that the field has required the development of powerful new
computers; novel mathematical algorithms for searching and analysing the databases;
and statistical analysis techniques. All new DNA sequences are deposited in the
international database GenBank, which is maintained by the US National Centre for
Biotechnology Information (NCBI). Anyone can access and search GenBank, and NCBI
provide numerous tools for searching and analysing sequence data.
Some of the goals of bioinformatics are:
 to assemble large complete chromosome sequences from the sequences of the short fragments used in
sequencing machines.
 to compare the DNA sequences of different species, in order to draw phylogenetic trees and deduce
evolutionary history.
 to find genes within a sequence of DNA, by comparison with known genes.
 to predict the amino acid sequence of proteins from their gene sequence and so predict the protein’s
structure and even function.
 to develop interesting and informative new ways of visualising all this complex data.
 to design new drugs and enzyme inhibitors
DNA and human classification
Comparison of DNA sequences have led to a new phylogeny for humans. It used to be thought that
humans were separate from all other apes, but we now know that humans are closely related to
chimpanzees, and indeed some taxonomists think that humans should be reclassified as the "third
chimpanzee":
HGS Biology A-level notes
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page 80
Classifying Life on Earth
Many different systems have been tried to classify life on Earth as this chart shows:
1735
Linnaeus
1866
Haeckel
1938
Copeland
1969
Whittaker
1990
Woese
2 kingdoms
3 kingdoms
4 kingdoms
5 kingdoms
3 domains
Monera
Prokaryota
Protoctista
Protoctista
Protista
Fungi
Fungi
Plantae
Plantae
Animalia
Animalia
Protista
Vegetabile
Plantae
Plantae
Animale
Animalia
Animalia
Bacteria
Bacteria
Archaebacteria
Archaea
Eucarya
From Greek times until the 19th century, living things have been divided into just two groups: animals (if
they moved) and vegetables (if they didn’t). The great German biologist Haeckel first introduced a kingdom
of microbes (called Protista), and in the 20th century the division between prokaryotes and eukaryotes was
finally recognised. In the 1960s the American biologist Whittaker introduced a 5 kingdom system, which
has proved to be influential and is still the system most used in biology education.
Five Kingdoms
The five kingdoms are: prokaryotae (or monera), protoctista (or protista), fungi, plantae and animalia. This
table summarises the main features of each kingdom, and the diagram on the next page shows a few of the
main phyla. The three “higher” kingdoms are defined mainly by their niches: absorption for fungi,
production for plants and consumption for animals. The protoctista kingdom is not well defined, and simply
contains all the eukaryotes that aren’t animals, plants or fungi.
Kingdom
Prokaryotae
Kingdom
Protoctista
Kingdom
Fungi
Kingdom
Plantae
Kingdom
Animalia
prokaryotic
eukaryotic
eukaryotic
eukaryotic
eukaryotic
Body form
unicellular or
colonial
unicellular or
multicellular
mostly
multicellular
multicellular
with
differentiation
multicellular
with extensive
differentiation
Cell wall
peptidoglycan
varied
chitin
cellulose
none
Nutrition
very varied
varied
absorption
photosynthesis
ingestion
absent
absent
absent
absent
present
present in some
present in some
absent
absent
present
Characteristic
Cell type
Nervous system
Motility
Named species
4,000
81,000
72,000
270,000
1,300,000
Est total species
1,000,000
600,000
1,500,000
320,000
10,000,000
HGS Biology A-level notes
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HGS Biology A-level notes
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Three Domains
In the 1970s the American microbiologist Carl Woese realised that there was enormous diversity within
the prokaryote kingdom and proposed that it be split in two. Furthermore the differences between the two
groups of prokaryotes were even bigger than the differences between the prokaryotes and the eukaryotes.
Woese therefore proposed that all life should be divided into three domains: The Archaea (or ancient life);
the Bacteria (or true bacteria) and the Eukarya (or eukaryotes).
In the simplest version of the three domain classification (shown above), the domains Archaea and Bacteria
contain one kingdom each, while the domain Eukarya contains the four eukaryotic kingdoms. However,
DNA studies are showing that few of the traditional kingdoms are phylogenetically correct, so biologists
are moving to a more accurate phylogenetic tree like this:
The principle differences between the three domains are summarised in this table:
Bacteria
Archaea
Eukarya
nucleus and organelles
none
none
present
Ribosomes
70S
70S
80S
circular without histones
circular with histones
linear with histones
no
sometimes
frequent
formyl-methionine
methionine
methionine
D-glycerol, ester link
L-glycerol, ether link
D-glycerol, ester link
peptidoglycan
proteinaceous S-layer
cellulose/chitin
DNA
introns
initiator amino acid
membrane phospholipids
cell wall
HGS Biology A-level notes
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AS Biology Unit 1
page 83
The Archaea
The initial classification of the Archea as a separate domain was based on the sequences of the 16S rRNA
gene, which was very different from both eukaryotes and bacteria. Further study has shown that the
Archaea share some features with bacteria (they’re both prokaryotes and have 70S ribosomes) and some
features with the Eukarya (they use the same enzymes for transcription and translation). But some features
are unique to the Archaea domain. For example the Archaea have a unique phospholipid in their cell
membranes:
Many of the Archaea are extremophiles i.e. they live in extreme environments. They include:
Thermophiles
Cells that live in environments hotter than 60°C, such as hot springs and deep-sea
thermal vents. The current record for the hottest growth temperature is 122°C, for
Methanopyrus kandleri.
Psychrophiles
Cells that live in environments colder than -15°C, such as permafrost and polar ice.
Halophiles
Cells that live in environments with a salt concentration higher than 15% (compared
to 3% in seawater), such as the Dead Sea in Israel and the Great Salt Lake in Utah.
Acidophiles and
Alkaliphiles
Cells that live in environments with pH<3 or pH>9 respectively.
Barophiles
Cells that live in environments under high pressure, such as deep oceans with
pressures exceeding 300 atmospheres.
Xerophiles
Cells that live in very dry environments, such as arid desert soils and rocks.
The Archea play an important role in both the carbon and nitrogen cycles. The nitrogen fixing bacteria and
the nitrifying bacteria are Archeans. The methanogens use a unique respiration that reduces carbon dioxide
to methane using hydrogen gas.
CO2 + 4H2 → CH4 + 2H2O
These methanogens are obligate anaerobes, poisoned by traces of oxygen. They are found in swamps,
marshes, and the intestines of mammals, and are responsible for creating natural gas and the methane gas
that is currently contributing to the greenhouse effect.
HGS Biology A-level notes
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AS Biology Unit 1
page 84
Evolution and Natural Selection
We have seen that there are millions of different species currently living on our planet. Each species is
different and has found a unique way of living; of solving the problems of surviving and reproducing
successfully. Where did all this diversity come from?
17th Century
Most people believed in Creationism, which considered that all life was created just as it
is now. This was not based on any evidence, but was instead a belief.
18th Century
Naturalists began systematic classification systems and noticed that groups of living
things had similar characteristics and appeared to be related. So their classifications
looked a bit like family trees.
European naturalists travelled more widely and discovered more fossils, which clearly
showed that living things had changed over time, so were not always the same.
Extinctions were also observed (e.g. dodo), so species were not fixed.
Selective
breeding was widely practised and it was realised that species (like crops, working dogs,
racing pigeons) could be changed dramatically by selection.
19th Century
Lamark (1809) proposed a hypothesis that living things changed by inheriting acquired
characteristics. e.g. giraffes stretched their necks to reach food, and their offspring
inherited stretched necks. This is now known to be wrong, since many experiments (and
experience) have shown that acquired characteristics are not inherited. Nevertheless
Lamark's theory was one of the first to admit that species changed, and to try to explain
the change.
Charles Darwin (1859) published "On the origin of
species by means of natural selection, or the preservation of
favoured races in the struggle for life", which proposed
the idea of natural selection, a far better explanation
for the changes in species. “Origin” has since been
recognised as one of the most important books ever
written. A very similar theory was also proposed by
Alfred Wallace, and Darwin and Wallace agreed to
publish at the same time.
20th Century
Mendel’s work on genetics was rediscovered and combined with Darwin’s theory to
form modern Darwinism. Many new techniques, like fossil dating, DNA sequencing,
molecular biology, microbiology and mathematical modelling, gradually formed an
extensive and overwhelming body of experimental evidence for Darwinism.
HGS Biology A-level notes
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AS Biology Unit 1
page 85
Darwin's Theory of Evolution by Natural Selection
Darwin observed that most organisms have far more offspring than survive to maturity – most organisms
die young from predation, disease and competition, so that populations are usually fairly constant in size.
Darwin realised that the organisms that die young were not random, but were selected by their
characteristics. He concluded that individuals that were better adapted to their environment compete
better than the others, survive longer and reproduce more, so passing on more of their successful genes to
the next generation.
Darwin explained the giraffe's long neck as follows:
1. In a population of horse-like animals there would be random genetic variation in neck length.
2. Animals with long necks were slightly better adapted as they could reach more leaves, and so were
better-nourished. These longer-necked animals lived longer, through more breeding seasons, and so had
more offspring.
3. The shorter-necked animals would be more likely to lose the competition for food, so would be poorly
nourished and would probably die young from predation or disease. They would have few, if any,
offspring.
4. So in the next generation there were more long-neck alleles than short-neck alleles in the population. If
this continued over very many generations, then in time the frequency of long-neck alleles would
increase and so the average neck length would increase.
[Today it is thought more likely that the selection was for long legs to run away from predators faster, and
if you have long legs you need a long neck to be able to drink. But the process of selection is just the same.]
Darwin wasn't the first to suggest evolution of species, but he was the first to suggest a plausible
mechanism for the evolution - natural selection, and to provide a wealth of evidence for it. Darwin used
the analogy of selective breeding (or artificial selection) to explain natural selection. In selective breeding,
desirable characteristics are chosen by humans, and only those individuals with the best characteristics are
used for breeding. In this way species can be changed over a period of time. All domesticated species of
animal and plant have been selectively bred like this, often for thousands of years, so that most of the
animals and plants we are most familiar with are not really natural and are nothing like their wild relatives
(if any exist). The analogy between artificial and natural selection is a very good one, but there is one
important difference - Humans have a goal in mind; nature does not.
Summary of Natural Selection
1. There is genetic variation in a characteristics within a population
2. Individuals with characteristics that make them less well adapted to their environment will die young
from predation, disease or competition.
3. Individuals with characteristics that make them well adapted to their environment will survive and
reproduce.
4. The allele frequency will change in each generation.
HGS Biology A-level notes
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AS Biology Unit 1
page 86
Natural Selection and Adaptation
The result of Natural Selection is that organisms who survive are well-adapted to their ecological niche. A
species’ niche means its role in its particular habitat. This role might include its position in the food chain
(producer, herbivore, predator, parasite); its use of resources (e.g. to build nests); its method of
reproduction (e.g. coloured flowers to attract insects); its contribution to matter cycles (e.g. nitrogen
fixing, decomposer); etc.
Successful organisms have features (called adaptations or adaptive traits) that make them good at surviving
in their niche, because natural selection has selected for these traits, so these traits help the organism to
survive and reproduce. These adaptive traits can take different forms:
 Anatomical adaptations. For example a cactus plant has tiny spine-shaped leaves, large roots and a
swollen stem to allow it to survive in a dry habitat.
 Physiological adaptations. For example the thermophilic archaea have special proteins and
membrane lipids that are stable at high temperatures and do not denature like normal proteins.
 Behavioural adaptations. For example the mating behaviour of many birds (including singing, dancing
and nest-building) help them to find a mate and so reproduce successfully.
All these adaptations arose by means of natural selection. Note that you can’t say that an individual
organism adapts to its surroundings in the way that a human might adapt to a new job. The process of
biological adaptation (developing adaptive traits like wings) applies to whole populations and takes place
gradually over long, evolutionary timescales of hundreds of thousands of years. An organism can be adapted
to its niche, or have an adaptation, but it can’t become adapted, and certainly not in its lifetime.
Adaptive traits give the appearance of design, but this is misleading and in fact close examination often
reveal design flaws. For example the vertebrate eye is an anatomical adaptation that has the appearance of
being designed, but the retina is actually rather poor, with an unnecessary blind spot. The explanation for
this poor design is that the eye was created by evolution from simpler structures, not designed from
scratch.
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page 87
Examples of Natural Selection
We’ll look at some examples of natural selection in action. In fact most things you’ll study in the biology
course (like protein structure, lung anatomy, the nitrogen cycle, disease, anything) are examples of natural
selection. It has been said that nothing in biology makes sense, except in the light of evolution.
Bacterial resistance to antibiotics.
We learnt in Unit 2 how antibiotics kill bacteria, but now some antibiotics no longer work because the
bacteria are resistant to them. This resistance is a good example of natural selection. Sometimes, at
random, mutations occur when bacterial DNA is replicated. These mutations may have any effect (and
most will be fatal), but just occasionally a mutation occurs that makes that bacterium resistant to an
antibiotic. For example the mutation might modify an enzyme so that it can bind and hydrolyse penicillin – it
is a penicillinase enzyme. So this cell is now resistant to penicillin. What happens next?
Imagine the mutation happened in bacterial cell living in your
gut. The mutated cell will reproduce by binary fission and
pass on its resistance gene to its offspring, forming a new
strain of bacteria in your gut. If there is no antibiotic present
in your gut (most likely) this mutated strain may well die out
due to competition with all the other bacteria, and the
mutation will be lost again. However, if you are taking
penicillin, then penicillin will be present in the bacteria's
environment, and these mutated cells are now at a selective
advantage: the antibiotic kills all the normal bacterial cells,
leaving only the mutant cells alive. These cells can then
reproduce rapidly without competition and will colonise the
whole environment. The mutant cells have been selected by the environment and so the frequency of the
resistance allele in the population will increase. Remember that the original mutation is a random event, not
caused by the presence of the antibiotic, but the spread of the allele is due to selection by an antibiotic-rich
environment.
There are now so many strains of antibiotic-resistant bacteria that it is getting more difficult to treat
disease. One of the most famous is MRSA (methicillin-resistant Staphylococcus aureus), a bacterium
responsible for a variety of diseases from staphylococcal food poisoning to toxic shock syndrome, and now
resistant to penicillin, methicillin and vancomycin. These bacteria are effectively untreatable at present. The
best solution is to minimise the use of antibiotics so that the resistant strain has no selective advantage, and
may die out.
HGS Biology A-level notes
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page 88
The Peppered Moth.
These light-coloured moths are well camouflaged from bird predators against pale lichen-covered bark of
trees, while rare mutant dark moths are easily picked off. During the industrial revolution in the 19th
century, birch woods near industrial centres became black with pollution. In this changed environment the
black moths had a selective advantage and became the most common colour, while the pale moths were
easily predated and became rare. Kettlewell tested this by releasing dark and light moths in polluted and
unpolluted environments and observing selective predation. Since pollution has cleared up in the 20th
century the selection has revered again and pale moths are now favoured again over dark ones.
Lactose tolerance in humans.
Some people are lactose intolerant, and feel ill (including diarrhoea and vomiting) when they drink milk. In
fact globally most human adults are lactose intolerant and this is the normal condition: lactose tolerance in
adults is a mutation. All infant mammals make lactase to digest lactose in their mother’s milk, and they all
stop producing lactase after they are weaned (its production is switched off at about age four in most
humans).
Around 10,000 years ago humans gradually changed from being mainly hunter-gatherers to being mainly
farmers, and for the first time animal milk was available as a food source. Humans who, through a chance
mutation, could drink milk without feeling ill were at an advantage, as they could supplement their normal
diet with milk in harsh times (and farming was very unreliable in the early days). By natural selection they
survived and their genes spread in their populations. As a result in human societies that adopted pastoral
(animal) farming (such as most Europeans, northern Indians and some Africans) are generally lactose
tolerant today, while the rest (most Asians, Africans, native Americans and Australians) remain lactose
intolerant as adults.
HGS Biology A-level notes
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page 89
HIV resistance in humans.
The AIDS virus HIV first arose in human populations in the 1930s in West Africa, where it spread from
primates through the practice of killing and eating “bush meat”. Since then it has gradually spread around
the world. Why is HIV so fatal to humans, but has so little effect on chimps? It turns out that chimps are
resistant because they have a protein (called CCL3) that stops HIV entering and infecting white blood cells.
Now some humans have this protein too, and it seems that the more copies of the gene for CCL3 you
have, the more resistant you are to
HIV. Chimps have on average 11 copies
non-African
humans
of the CCL3 gene, African humans have
Africa people who, by chance, have
many copies are favoured and will
Frequency
on average 6 copies, and non-African
humans have on average 2 copies. In
chimps
African
humans
reproduce, while those with few copies
die young without reproducing. So
natural selection in humans explains the
frequency of the CCL3 gene. A
thousand years in the future, if we have
0
2
4
6
8
10
12
14
16
Number of copies of CCL3L gene
not developed a medical cure for HIV, the whole human population will probably have evolved to possess
around 11 copies of CCL3.
HGS Biology A-level notes
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page 90
Types of Natural Selection
Populations change over time as their environment changes. These changes can be recorded as changing
histograms of a particular phenotype (which of course is due to changes in the underlying alleles). These
histograms show three kinds of natural selection, depending on which phenotypes are selected by the
environment. The shaded areas represent the phenotypes that are favoured.
Allele frequency
Allele frequency
Measure of phenotype
Measure of phenotype
Measure of phenotype
Allele frequency
Allele frequency
Allele frequency
Measure of phenotype
Stabilising Selection
Allele frequency
Disruptive Selection
Directional Selection
Measure of phenotype
Measure of phenotype
 Directional Selection occurs when one extreme phonotype (e.g. tallest) is favoured over the other
extreme (e.g. shortest). This happens when the environment changes in a particular way. "Environment"
includes biotic as well as abiotic factors, so organisms evolve in response to each other. e.g. if predators
run faster there is selective pressure for prey to run faster, or if one tree species grows taller, there is
selective pressure for other to grow tall. Most environments do change (e.g. due to migration of new
species, or natural catastrophes, or climate change, or to sea level change, or continental drift, etc.), so
directional selection is common.
 Disruptive (or Diverging) Selection. This occurs when both extremes of phenotype are selected over
intermediate types. For example in a population of finches, birds with large and small beaks feed on large
and small seeds respectively and both do well, but birds with intermediate beaks have no advantage, and
are selected against.
 Stabilising (or Normalising) Selection. This occurs when the intermediate phenotype is selected over
extreme phenotypes, and tends to occur when the environment doesn't change much. For example
birds’ eggs and human babies of intermediate birth weight are most likely to survive. Natural selection
doesn't have to cause a directional change, and if an environment doesn't change there is no pressure
for a well-adapted species to change. Fossils suggest that many species remain unchanged for long
periods of geological time.
HGS Biology A-level notes
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page 91
Speciation – The Origin of New Species
We’ve seen how species can change through natural selection, but how does this change lead to new
species? The simple answer is that new species are formed when an existing species splits into two separate
groups. For the two groups to become two species they must be reproductively isolated:
If members of the two groups can still interbreed If interbreeding between the two groups is
then there will be gene flow between them and they somehow prevented, then there is no gene flow and
will not be reproductively isolated. They will remain the two groups are reproductively isolated. They
members of the same species.
could become different species.
Reproductive Isolating Mechanisms
How can two groups of organisms be reproductively isolated? There are many different mechanisms:
 Geographical isolation. Populations obviously can’t interbreed of they can’t reach each other,
because they’re separated by oceans, mountains, rivers, deserts, land masses, etc.
 Ecological isolation. Populations can be isolated if they have different niches within the same area. For
example in India lions hunt in open grassland, while tigers hunt in forests.
 Behavioural isolation. This particularly applies to courtship behaviour, which allows members of the
same species to identify themselves and synchronise mating. For example female birds will only mate
with male birds with the correct plumage, song and dance.
 Mechanical isolation. Animals can’t interbreed if they can’t physically mate, and even plants can’t
interbreed if their flowers are different shapes so the same insects can’t visit them to transfer pollen.
 Temporal isolation. Many plants and animals have reproductive seasons and if these seasons don’t
match then breeding is impossible. For example plants that flower at different times, insects that pupate
at different times or mammals that come into oestrus at different times.
 Genetic isolation. This covers a range of developmental problems that occur even if fertilisation can
take place between two different species. In most of these cases the embryo will fail to develop beyond
a certain stage because the genes are somehow incompatible. The chromosomes may not replicate;
mitosis may not take place correctly or genes required for embryo development may not be present.
Even if a hybrid is born it may be weak and nor survive, or it will be sterile, like the mule.
There are two ways that speciation can come about: allopatric speciation and sympatric speciation.
HGS Biology A-level notes
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page 92
Allopatric Speciation
Allopatric means “different fatherland”, and allopatric speciation happens when two groups become
geographically separated. It is by far the most common kind of speciation.
1. Start with an interbreeding population of one species.
2. The population becomes divided by a physical barrier such as water,
mountains, desert, or just a large distance. This can happen in various ways:
 When some of the population migrates or is dispersed to a new area, such
as an isolated island or the other side of a mountain or large river. The
dispersal must be over an unusually large distance so that it is not
repeated and the new group remains isolated. Land birds, insect and plant
seeds can be carried unusually large distances in a storm and small animals
can unintentionally “raft” across oceans on fallen trees. Isolated islands like
Madagascar, Hawaii and the Galapagos were colonised this way.
 When the geography changes catastrophically e.g. an earthquake could
create an impassable rift; the lava flow from a volcano could create an
impassable barrier, rising sea level could create islands; or falling sea level
could isolate lakes.
 When the geography changes gradually e.g. erosion could create a deep
valley like the Grand Canyon; plate tectonic movements cause whole
continents to move apart (which explains why similar species are found in
South America and Africa).
The isolated population could be as small as a few seeds or a single pregnant
female. The populations must be reproductively isolated, so that there is no
gene flow between the groups.
3. If the environments (abiotic or biotic) are different in the two places (and
they almost certainly will be), then different characteristics will be selected by
natural selection and the two populations will evolve differently. Even if the
environments are similar, the populations may still change by random genetic
drift, especially if the population is small. The allele frequencies in the two
populations will become different.
4. Much later, if the barrier is now removed and the two populations meet
again, they are now so different that they can no longer interbreed. They
therefore remain reproductively isolated and are two distinct species. They
may both be different from the original species, if it still exists elsewhere.
HGS Biology A-level notes
NCM/2/16
AS Biology Unit 1
page 93
Sympatric Speciation
Sympatric means “same fatherland”, and sympatric speciation happens when two groups become
reproductively isolated even though they share the same geographical location. It is much rarer than
allopatric speciation, but there are some examples, mainly in plants.
Sympatric speciation in plants is based on three processes; hybridisation, polyploidy and self-fertilisation.
Firstly the gametes of two different species fertilise each other to form a hybrid (or mixed) plant. This
hybrid plant is not viable (i.e. it’s sterile) because the chromosomes from different species don’t form
homologous pairs. This means bivalents can’t form, meiosis can’t take place and gametes can’t be made.
Mitosis can take place, so the plant can grow. Now suppose that complete nondisjunction takes place in
one cell division so that the chromosome number is doubled (see pxx). This new cell now has matching
homologous chromosomes, so it can undergo meiosis and it can make gametes. These gametes can only
fertilise gametes from the same plant, but that’s not a problem since plants can self-fertilise, since they are
hermaphrodites (pxx). So, in one step a new species has been made that can reproduce with itself but not
with any other species. This is how hexaploid wheat was formed.
Sympatric speciation in animals is rarer, but one well-described example is the fruit fly Rhagoletis pomonella
in the United States. The original species lives on hawthorn bushes, but a new population has arisen that
feeds on apple trees instead. This new population arose from a mutant fly that was able to digest apples and
now the population of “apple flies” has a new niche in apple orchards so are effectively isolated from the
“hawthorn flies”. This isolation means the two populations are evolving independently and have already
altered their breeding seasons to match the different fruiting seasons of the two plants. They have become
two separate species in the same geographical area.
HGS Biology A-level notes
NCM/2/16
AS Biology Unit 1
page 94
Summary of Speciation
1. A population becomes separated into two groups that are reproductively isolated, so that there is no
gene flow between the groups. The isolation can be geographical (allopatric) or some other method
(sympatric).
2. The two groups’ environments are different, so natural selection favours different characteristics.
3. The allele frequencies in the two groups will change in different ways.
4. Eventually the two populations will be unable to interbreed, so will be different species.
Populations of the same species that are currently isolated are called subspecies (or sometimes breeds,
varieties or races) and they may in time become distinct species, or they may remain an interbreeding
single species. It is meaningless to say that one species is absolutely better than another species, only that it
is better adapted to that particular environment. A species may be well-adapted to its environment, but if
the environment changes, then the species must evolve or die. In either case the original species will
become extinct. Since all environments change eventually, it is the fate of all species to become extinct
(including our own).
Deep Time and the Origin of Life
It takes time to evolve 100 million species and it is now known that the earth is 4,600 million years old. Life
(in the form of prokaryotic cells) arose quite quickly, and has existed for around 4,000 million years. These
huge spans of time are almost impossible to comprehend, and are often referred to as deep time. This
chart illustrates some of the events in the history of the Earth.
No one knows how life arose in the first place, but the conditions in the early Earth were very different
from now, and experiments have shown that biochemicals like amino acids and nucleotides could be
synthesised from inorganic molecules under primordial conditions. We saw on pxx how lipid bilayers can
form spontaneously, so perhaps that’s how cellular life arose.
HGS Biology A-level notes
NCM/2/16
AS Biology Unit 1
page 95
Biodiversity
Biodiversity simply means the variety of all the life on Earth. The 1992 United Nations Earth Summit in Rio
de Janeiro defined biodiversity as "the variability among living organisms from all sources, including, 'inter alia',
terrestrial, marine, and other aquatic ecosystems, and the ecological complexes of which they are part: this includes
diversity within species, between species and of ecosystems". This definition is adopted by the United Nations
Convention on Biological Diversity. There are thus three levels of biodiversity:
All three levels of biodiversity are important because all living organisms are inter-related and depend upon
each other in numerous ways. All three levels are also linked, for example genetic diversity is necessary to
maintain species diversity, and vice versa.
Conservation
Human activities are reducing biodiversity at all three levels. Ecosystem diversity is reduced by
deforestation, mining, and building; species diversity is reduced by farming, hunting and habitat destruction;
and genetic diversity is reduced by selective breeding and competition with humans. Understanding our
impact on biodiversity has led to conservation – the attempt to conserve biodiversity worldwide.
Conservation applies to all three levels. The global gene pool is a resource for learning more about life on
Earth, and some genes may be able to provide us with useful products for medicine and biotechnology. To
maintain the gene pool we need to preserve species diversity and to conserve species diversity we must
provide suitable niches for all species by preserving habitat diversity.
Conservationists recognise that humans need to exploit the natural world in order to survive. So the aim of
conservation today is not to preserve pristine untouched nature, but is the management of our
environment to keep the land as a productive resource, but in a sustainable way that maintains biodiversity
and will continue to do so in the future for all our descendants.
HGS Biology A-level notes
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AS Biology Unit 1
page 96
Justifying Conservation
Why is conservation important? Some reasons are based on the ethics of whether particular actions are
right or wrong, while other reasons are based on the practicalities of benefits to humans. In conservation
there are no simple answers and decisions are based on a balance between opposing views, for example:

the needs of humans to use and exploit nature for our survival

the needs of other animals to live their lives without human interference

the needs of humans to enjoy the natural beauty of untouched nature

the needs of our descendants to enjoy or use ecosystems as we do

the needs of everyone on the planet to avoid catastrophes such as global warming or mass extinctions
Conservation is expensive, and, in order to persuade governments and businesses to take an interest, the
concept of Ecosystem Services has been devised. The aim of ecosystem services is to teach politicians and
other non-scientists some basic ecology and make them more aware of the benefits of the natural world
and so hopefully to encourage them to put effort and money into conserving them. Ecosystem services are
grouped into four categories:
1. Supporting services. These include the basic functions of all ecosystems, such as nutrient cycling (the
carbon cycle, nitrogen cycle, water cycle, etc.), primary production (i.e. CO2 fixing by photosynthesis),
decay by decomposers and soil formation. These functions underlie everything else.
2. Regulating services. These are also basic functions of all ecosystems, framed so as to emphasise their
importance in regulating the natural world. They include:

the carbon cycle, which “regulates” the carbon dioxide concentration in the air, and so has an
impact on climate change.

decay, which gets rid of organic “waste” and “toxins”. Decomposers are used for example in
sewage treatment to provide clean water.

population dynamics, such as predator-prey interactions, which “regulate” pest and pathogen
populations.
3. Provisioning services. These are all the useful products humans obtain from the natural world. There
are of course millions of these, including:

food, including farmed food and wild food.

water

raw materials for building, clothing and industry.

minerals, including fossil fuels, metal ores, jewels, raw materials for the chemical industry.

medicinal resources, including pharmaceuticals and microbes

genetic resources, i.e. genes that can be used in biotechnology

energy, including biomass fuels, natural gas, hydro-electric power, etc.
4. Cultural services. These are the non-material benefits nature provides for people, including recreation
(e.g. sports and tourism); education and cultural (e.g. inspiration for literature or art).
HGS Biology A-level notes
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AS Biology Unit 1
page 97
In-Situ Conservation
This means conserving whole natural ecosystems and the various species they contain. In practice this
means setting up Protected Areas, such as National Parks. In-Situ Conservation automatically means
protecting all three levels of biodiversity – ecosystem, species and genetic.
The International Union for the Conservation of Nature (IUCN) oversees the organisation and
classification of Protected Areas worldwide. Almost every country in the world has set up Protected Areas
to conserve local habitats and their wildlife. There are over 161,000 protected areas in the world with
more added daily, representing about 15% of the world's land surface area and 1% of the world's oceans.
There are laws forbidding or controlling human activities like building, hunting, farming, mining, industry and
other exploitation of Protected Areas, though the specific limitations vary enormously depending of the
area. Tourism and education are generally encouraged, though with strict controls.
In the UK Protected Areas include: National Parks (NP); Environmentally Sensitive Areas (ESA); Sites of
Special Scientific Interest (SSSI); Marine Nature Reserves (MNR); National Nature Reserves (NNR); Local
Nature Reserves; Ramsar Wetland Sites (RWS); and World Heritage Sites (WHS). There are 15 National
Parks in the UK, and, in contrast to National Parks in other countries, considerable human activity is
allowed in National Parks, including farming and towns. The aim is to allow economic development while
still protecting the countryside.
Ex-Situ Conservation
This means conserving species away from their natural habitats. In practice this means collecting specimens
in zoos, aquaria, botanical gardens, seed banks and gene banks. Zoos were originally established for
entertainment, but now serve the more useful functions of education and conservation. International
breeding programs allow endangered species to reproduce safely in captivity and, where possible, released
into the wild.
Endangered plants may be grown in botanical gardens, and can also be preserved in seed banks, where
samples of seeds are dried and preserved cryogenically at -40°C. Large numbers of seeds can be preserved
this way for centuries without losing their fertility. The Millennium Seed Bank (MSB), run by the Royal
Botanic Gardens, Kew, currently (2015) holds nearly 2 billion seeds from 34,088 wild plant species. Their
aim is to conserve the seeds from 75,000 species of plants (25% of known plants) by 2020, including all the
UK’s native plants. There is now an attempt to preserve endangered animal species in gene banks, by
storing embryos cryogenically.
HGS Biology A-level notes
NCM/2/16
AS Biology Unit 1
page 98
Genetic Diversity
Genetic diversity means the variety of alleles within a species’ gene pool. Members of the same species all
have the same genes, but different combinations of alleles. New alleles arise through mutation and existing
alleles are recombined by meiosis and random fertilisation during sexual reproduction so that every
individual within a species is genetically unique. The number of different alleles present within a species is
called the genetic diversity of the species. By using modern genome sequencing techniques on a sample of
individuals in a population we can actually quantify the genetic diversity.
Genetic diversity is important because it is the basis of evolution and survival of a species. A species with a
high genetic diversity is likely to have some individuals with the characteristics required to survive a change
in the environment, so some members of the species will survive. Low genetic diversity means a species is
more likely to become extinct due to environmental changes. Genetic diversity is generally higher in large
populations and lower in small populations. Some populations have very low genetic diversity, due to
natural or human causes.
One cause of low genetic diversity is a genetic bottleneck, which happens when a population is drastically
reduced in size (perhaps due to a natural catastrophe or when a few individuals colonise an island). The few
individuals left will only have a small range of alleles between them, so if they reproduce and the population
increases again there will be reduced genetic diversity. Many of the original variety of alleles will have been
lost in individuals who didn’t survive. Cheetahs are a threatened species partly due to their very low genetic
diversity, probably due to a genetic bottleneck at the end of the last glacial period ten thousand years ago.
Selective breeding of farm animals and crops also leads to reduced genetic diversity, since only a few useful
characteristics (and therefore alleles) are chosen. Conservation efforts aim to increase genetic diversity.
HGS Biology A-level notes
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AS Biology Unit 1
page 99
Species Diversity
All the organisms living in a habitat are collectively called its community, and species diversity means the
variety of species in a community. Species diversity is useful because it tell us about the complexity, quality
and stability of an ecosystem.
In order to measure species diversity we need to take samples of a population. For example we could place
a number of random quadrats in the area. The same sampling technique must be used in all areas that are
to be compared. The simplest measurement is just to count the number of species in the samples - the
species richness. However richness alone is not a good measure of diversity because it doesn’t take into
account the size of each species population – its abundance. For example a wild meadow and a wheat field
might both have 25 species, but in the meadow the species are equally abundant, while in the wheat field
95% of all the plants are the single species of wheat. A good measure of diversity takes into account the
species richness and their abundance. One common measure is the Simpson Diversity Index (D):
Simpson
Diversity
Index
D
N(N  1)
 n( n  1)
where N = total number of individuals (total abundance)
n = number of individuals in each species
The higher the index, the higher the species diversity. A community where one species is dominant over
others has a lower diversity than one where the species are more equitable. For example these two
communities each have 100 individuals in 3 species:
(a)
species
A
B
C
total
abundance
90
5
5
100
n(n-1)
8010
20
20
D
100  99
8050
 1.23
8050
(b)
species
abundance
n(n-1)
A
34
1122
100  99
D
 3.06
B
33
1056
3234
C
33
1056
total
100
3234
So (b) is more diverse than (a). A few dominant species tend to decrease the diversity index.
Harsh habitats tend to have low species diversity, as only a few species are adapted to the harsh
environment. Mild habitats support a high species diversity. The many plant species will lead to high
productivity (due to a lot of photosynthesis), which will support a large, complex food web.
HGS Biology A-level notes
NCM/2/16