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Download Add 200 mg of proteinase K to a 15ml polypropylene tube - IB-USP
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Transcript
20 MG/ML PROTEINASE K Add 200 mg of proteinase K to a 15ml polypropylene tube containing 9,5ml of H2O. To reduce denaturation, always add protein to an aqueous solution instead of adding an aqueous solution to a protein. Gently rock the capped tube until the proteinase K is completely dissolved. Do not mix by vortexing (this causes foaming, which indicates protein denaturation). Adjust the final volume to 10 ml. Divide into aliquots and store at -20°C. In general, this solution is not sterilized.
