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This site contains high quality jpeg images (~1-2Mb each) of each scanned in situ hybridization slide as they will appear in the Jackson Lab Gene Expression Database. Full resolution, lossless jpeg2000 images (~20Mb each) for each image will be made available for download on our local server, but require ~150 Gb of storage and were hence not feasible for review purposes. The images were acquired using a Nikon Coolscan film scanner. This does not have the dynamic range of a microscope. In order to more easily see localized expression for some genes, it is useful to open the files in Photoshop and modify their levels (under image adjustments: autolevel). The naming conventions of the image files are as follows. Whole mount e10.5 in situ hybridization: Example: 0967d = XXXX P XXXX= MTF number (0967) P – Permeablization conditions a = condition 1 (3’ proteinase K treatment) b = condition 2 (30’ proteinase K treatment) d = One embryo at condition 1 and the other at condition 2 Slide sections e13.5, P0, and cerebellum P7, P15, and P21: Example: T0042111000 = T XXXX YZA BLL T = Mahoney Transcription Factor (MTF) XXXX= MTF number Y – Mouse stage 1 = E13.5 2 = P0 3 = Cerebellum at different stages (all on same slide) P7 P15 P21 4 = E10.5 whole mount Z – Slide number for brains that require more than one slide to complete 1 = rostral 2 = caudal 3 = further caudal A – replicate number Some genes have multiple replicates B – not used LL – image type aa jpeg 00 jpeg2000 included here quality 10; size 100% Lossless and large in size, but they are not