Download Role of the Neural Crest in Vertebrates Development and Evolution

iBioSeminars: Nicole Le Douarin,
March 2008
Role of the Neural Crest in Vertebrates
Development and Evolution
ROLE of the NEURAL CREST
in
Vertebrates Development and Evolution
Nicole LE DOUARIN
CNRS and
Collège de France
PARIS
The pioneer of the neural crest
(1868) Wilhem His (1868) In chick
A band of particular material lying between the neural tube and the superficial ectoderm
‘ Zwischenstrang ’
Cells migrating from the neural primordium aggregate to form the dorsal root ganglia
‘Ganglionic crest’
The American Society for Cell Biology
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iBioSeminars: Nicole Le Douarin,
March 2008
Role of the Neural Crest in Vertebrates
Development and Evolution
Tracing the migratory cells
in the developing avian embryo
by constructing Quail-Chick Chimeras
Feulgen staining of DNA
Q
C
Q
The QCPN Mab
The American Society for Cell Biology
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iBioSeminars: Nicole Le Douarin,
March 2008
Role of the Neural Crest in Vertebrates
Development and Evolution
THE NEURAL CREST
CONTRIBUTION
to VERTEBRATES ONTOGENY and
EVOLUTIONARY SIGNIFICANCE
The Neural Crest (NC) is part
of the neural primordium
Dorsal
ectoderm
Neural
fold
Neural
epithelium
Cephalic neural plate
Truncal NT
Early neural crest cells
10 ss
5‐ss
Marker genes
Snail2/Slug
Sox8
Sox9
Sox10
FoxD3
Pax3
...
Cell surface marker
HNK1
The American Society for Cell Biology
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iBioSeminars: Nicole Le Douarin,
March 2008
Role of the Neural Crest in Vertebrates
Development and Evolution
GRAFTING PROCEDURE
The neural tube has been removed in the recipient embryo
Quail neural tube isolated before grafting
The American Society for Cell Biology
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iBioSeminars: Nicole Le Douarin,
March 2008
Role of the Neural Crest in Vertebrates
Development and Evolution
Quail Neural Tube grafted into a chick embryo
NCC migrating from the grafted quail Neural Tube
Feulgen staining
A Quail-Chick neural chimera
The American Society for Cell Biology
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iBioSeminars: Nicole Le Douarin,
March 2008
Role of the Neural Crest in Vertebrates
Development and Evolution
An adult Quail-Chick neural chimera
The derivatives of the neural crest
• Neurones :PNS sensory, sympathetic and enteric ganglia)
• Glial cells :PNS satellite glia and nerve Schwann cells
• Pigment cells
• Endocrine cells
Adrenal medulla
C cells of the thyroid
Cartilages and bones
• Mesectoderm
Dermis
Connective tissues
Vascular smooth muscle cells
Adipocytes
Diversity of NC-derived cell types
according to their level of origin along the neuraxis
The American Society for Cell Biology
6
iBioSeminars: Nicole Le Douarin,
March 2008
Role of the Neural Crest in Vertebrates
Development and Evolution
Origin of the autonomic nervous system
The suprarenal gland of a chimeric bird:The catecholamine-containing
cells are fluorescent and carry the quail nuclear marker
Quail to chick isotopic graft
of the “vagal” NT
QUAIL
CHICK
The American Society for Cell Biology
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iBioSeminars: Nicole Le Douarin,
March 2008
Role of the Neural Crest in Vertebrates
Development and Evolution
(E2.5)
(E2.5)
The American Society for Cell Biology
8