Download Unit 3 Exam Enzymes REVIEW

Unit 3 Exam: Tuesday, November 22nd: Enzymes:
REVIEW TOPICS: All questions be answered and must be turned in on the day of the test in order to be
allowed to do the test make-up.
Free Energy POGIL: Explain the Gibbs Free Energy equation. A reaction that increases entropy is/is not
(choose one) spontaneous? Explain why. Given a delta G less than 0, will the reaction release or absorb
energy? Explain why.
Metabolism: Compare anabolism and catabolism. Give an example of each. Explain how anabolic and
catabolic reactions differ in their transfer of energy. What is a metabolic pathway and how are enzymes
involved? What is the 1st law of thermodynamics? Give some examples from nature and class. What is the
2nd law of thermodynamics? Give some examples from nature and class.
Protein Structure (3.6): How is a protein polymerized? What makes 1 protein different than another?
Compare primary and tertiary structure. Why do some amino acids’ R groups interact with each other but
others don’t? Choose 3 amino acids to compare/contrast. What can cause R groups to no longer interact?
Sickle Cell Poster: Compare Hemoglobin A vs Hemoglobin S. Why is Hemoglobin S different and what
impact does this difference have on people with sickle cell anemia? Explain the role of genetics in causing
change to proteins.
Modeling Activity: Protein Structure: Explain how proteins fold. Explain why denaturation cannot be
undone. Compare the egg before and after heating. What has happened to the protein?
Enzyme Structure and Function (5.4 and 5.5): How do enzymes work? Why are enzymes substrate
specific? Use tertiary structure of a protein to explain. What environmental factors can influence the
shape of an enzyme? Explain how. Explain what the optimal environment is given the graph below.
Research each of these three enzymes and explain how their function matches their optimal pH.
Penny Flipase Modeling Activity: Explain the role of denaturation, coenzymes, and competitive inhibitors
in influencing enzymatic reaction rates. Calculate rate for time 0-5 and 10-20 for the 3 trends on the
graph below. Explain what’s happening at the molecular level during these two time periods for these 3
different environments.
Acids and Bases (2.6): What does an acid/base do to an enzyme? How? Draw 4 molecules in solution:
NaOH, HCl, vinegar, and ammonia. Explain how each caused a pH change in the solution. Explain how pH
is measured.
The Spit Lab: Part 1: Describe what a serial dilution is and what the value of it is. Explain how the
indicator iodine works to indicate the presence of starch. Use the equation for the line of the known
amylase concentrations to determine the unknown amylase concentration of a student’s spit where the
student’s spit digested 275mm2 of starch.
Area of Digested Starch (mm2)
350
y = 255x + 45.424
300
250
200
150
100
50
0
0
0.2
0.4
0.6
0.8
1
1.2
Concentration of Amylase (%)
The Spit Lab Part 2: Factors Affecting Enzyme Function: What factors influence enzyme shape? How can
this be measured? Use amylase, iodine, amylose, and maltose in your explanation.