Download Enzymes

Biology 20
Pre-Lab #5 Enzymes
Name:
Please type your answers below and submit them at the beginning of lab Tuesday.
Please read the lab and answer the following questions:
Transport Review:
1. What is osmosis?
2. For dialysis to occur, solutes move from a low concentration to higher concentration? True or False
3. In the experiment with the red agar blocks (small, medium or large):
A) which cell size obtained the MOST vinegar in 10 minutes
B) which cell size (small, medium or large) was the most efficient (no or very little red remaining)?
4. In the osmosis demo with the 10% and 60% glucose solutions, which one had the greatest amount of water that
diffused into the osmometer and WHY? This is a two part question.
5. Molecules diffuse from an area of [high]  [low] OR [low]  [high]? (note [ ] = concentration)
Enzymes Lab:
6. What is an enzyme?
7. What do enzymes work on? And where?
8. In Part A of the enzyme lab, which is the enzyme and which is the substrate (sucrose & sucrase)?
9. What do you need to do with the Benedict’s solution in order for it to work properly? Where will you dispose of
the used Benedict’s solution?
10. Will an enzymatic reaction take place in the test tube placed at 100°C? Why or why not?
11. Enzymes such as succinic acid dehydrogenase can be found in what specific location in which specific organelle?