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Toothpickase PreLab Resource: Toothpickase lab worksheet Pre-lab: Complete the following parts on a separate sheet of paper prior to conducting the laboratory. Part 1: Title Do not develop a title until you have completed the pre-lab. Then develop a title for this laboratory that is in the form of a question. Part 2: Objectives (write them down) By the end of this lab I will be able to: Explain the general functions and activities of enzymes; Identify the relationship between the structure and function of enzymes; Explain how changes in temperature, pH, enzyme concentration, and substrate concentration can affect the reaction rates of enzyme-catalyzed reactions; Explain how environmental factors affect the rate of enzyme-catalyzed reactions. Part 3: Pre-lab Questions (write the questions and the answers) 1) What is a very important role that enzymes have in cells? 2) Explain the participants in an enzyme catalyzed reaction. Diagram an enzyme and label the active site. 3) The following are factors that affect an enzyme’s rate of reaction. For each, explain how it interferes. a. pH b. temperature c. cofactors d.inhibitors 4) Write the chemical equation for the decomposition of a toothpick by Toothpickase. Part 4: Procedure Read the procedures carefully. Diagram the procedures in a flow chart (i.e. the grilled cheese). The flow chart should be detailed enough that an elementary school student can follow them and complete the lab.