Download Table S5. Proteins specifically induced or repressed during A

Supporting information
Figure S1. Analysis of the jr1 T-DNA insertion mutant used for the thermotolerance
assay.
(A) Genomic organization of JR1. Exons are indicated as rectangles. The triangle marks the
region of T-DNA insertion. (B) qPCR analysis of JR1 expression in Col-0 or in the JR1 mutant
line described in (A). Accumulation of the JR1 transcripts is expressed as fold change
values related to the control sample (Col-0), which was arbitrarily assigned to 1 after
normalization to TUB5.
Table S1. Primer sequences used for qRT-PCR analyses.
Table S2. Description of the reported thermotolerance phenotypes associated to gain
and loss of function mutants of some genes whose proteins show a significant change in
the iTRAQ analysis.
This table displays the AGI number of these identified proteins, the name of the proteins
according to TAIR, the number of peptides identified in each independent experiment, the
changes of protein level observed in the iTRAQ analysis (average ± standard deviation) and
a short description of the phenotypes observed under heat stress for the corresponding
lack or gain of function mutants. Significant changes among the two biological replicates
are highlighted in grey. (SAT) Short acquired thermotolerance, (HS) Heat stress, (BT) Basal
thermotolerance, (AT) Acquired thermotolerance, (HT) Heat tolerance, (LAT) Long-term
acquired thermotolerance.
Table S3– Proteins significantly induced and repressed during the general heat stress
response
The AGI number, the name of the protein according to TAIR description, the number of
peptides identified in each independent experiment and the protein quantification by
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iTRAQ (average ± standard deviation) are shown for each protein coordinately induced or
repressed during A, CS and CD compared to C. Only those proteins with a significant
change (|Zq|≥ 2) in both experiments are included.
Table S4. Proteins coordinately changed during A and CS and CS and CD, respectively.
The AGI number, the name of the protein according to TAIR description, the number of
peptides identified for each protein in each independent experiment and the fold change
of each protein in each condition compared to C (shown as average ± standard deviation)
are shown. Values of Zq ≤ -2 and Zq ≥ 2 that correspond to proteins induced or repressed
in each experiment are highlighted in red and blue, respectively. The intensity of the color
correlates with the change in the Zq value.
Table S5. Proteins specifically induced or repressed during A, CS or CD treatments.
The AGI number, the name of the protein according to TAIR description, the number of
peptides identified for each protein in each independent experiment and the fold change
of each protein in each condition compared to C (shown as average ± standard deviation)
are shown. Values of Zq ≤ -2 and Zq ≥ 2 that correspond to proteins induced or repressed
in each experiment are highlighted in red and blue, respectively. The intensity of the color
correlates with the change in the Zq value.
Table S6. Proteins significantly changed in the iTRAQ data and whose mRNA levels are
regulated by the HsfA2.
The AGI number, the name of the protein according to TAIR description, the number of
peptides identified for each protein in each independent experiment and the fold change
of each protein in each condition compared to C (shown as average ± standard deviation)
are shown.
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