Download Publication JournalArticle (Originalarbeit in einer wissenschaftlichen

Publication
The YPWM motif links Antennapedia to the basal transcriptional
machinery
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
ID 152848
Author(s) Prince, Frédéric; Katsuyama, Tomonori; Oshima, Yoshiteru; Plaza, Serge; Resendez-Perez, Diana;
Berry, Meera; Kurata, Shoichiro; Gehring, Walter J
Author(s) at UniBasel Gehring, Walter Jakob;
Year 2008
Title The YPWM motif links Antennapedia to the basal transcriptional machinery
Journal Development
Volume 135
Number 9
Pages / Article-Number 1669-79
Keywords ANTP, HOX, bip2, dTAF3, Drosophila, eye-to-wing transformation, homeotic transformation
HOX genes specify segment identity along the anteroposterior axis of the embryo. They code for transcription
factors harbouring the highly conserved homeodomain and a YPWM motif, situated amino terminally to it.
Despite their highly diverse functions in vivo, HOX proteins display similar biochemical properties in vitro,
raising the question of how this specificity is achieved. In our study, we investigated the importance of the
Antennapedia (Antp) YPWM motif for homeotic transformations in adult Drosophila. By ectopic
overexpression, the head structures of the fly can be transformed into structures of the second thoracic
segment, such as antenna into second leg, head capsule into thorax (notum) and eye into wing. We found that
the YPWM motif is absolutely required for the eye-to-wing transformation. Using the yeast two-hybrid system,
we were able to identify a novel ANTP-interacting protein, Bric-a-brac interacting protein 2 (BIP2), that
specifically interacts with the YPWM motif of ANTP in vitro, as well as in vivo, transforming eye to wing tissue.
BIP2 is a TATA-binding protein associated factor (also known as dTAFII3) that links ANTP to the basal
transcriptional machinery.
Publisher Company of Biologists
ISSN/ISBN 0950-1991
edoc-URL http://edoc.unibas.ch/dok/A5257274
Full Text on edoc
No
Digital Object Identifier DOI 10.1242/dev.018028
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/18367556
ISI-Number WOS:000254917900011
Document type (ISI) Journal Article