Download Production of : Enterovirus type 71 Virus using TideCell Bioreactor

Production of Enterovirus type
71 Virus using TideCell
Bioreactor System
Production case study using Vero Cells
(R1) High cell density is required to
achieve high titer, and reduce medium
consumption.
High surface area provided in TideCell
increases cell density up to 10 folds and
save culture medium up to 90%.
Roller bottle:low cell density due to
limitation of surface area
Microcarrier system: the bead density is
limited
Fixed bed system: scale is limited.
Hollow fiber system: scale is limited.
(R2) Cells tend to detach during postinfection period.
TideCell provides extremely low shear
stress culture environment in which cells
are not easy to detach after infection and
thus increase the productivity.
Other system: cells tend to detach due to
agitation or circulation resulted in higher
shear stress.
(R3) Infection time is crucial. Sampling of
carrier is required for cell count and
observation
Easy to take carrier sample for cell
counting and MOI calculation.
Other packed bed system cannot do
carrier sampling.
(R4) Require to adjust matrix to culture
medium ratio
Easy to do with TideCell for increasing cell
attachment and infection efficiency
Cannot do with all other systems
(R5) Require to reduce impurities to
increase the recovery rate.
Low shear in TideCell causes less
impurities and the matrix tends to entrap
the cell debris
Other agitated systems tend to disrupt the
cells and cause more impurities
3D growth of Vero Cell Culture
After infection
Results
TCID50: log 7.5
Microcarrier system: Log 7.0