Download Nguyễn Thị Trang1, Nguyễn Thị Giang1, Vũ Thị Thu Hiền1 1Viện Di

Nguyễn Thị Trang1, Nguyễn Thị Giang1, Vũ Thị Thu Hiền1
1
Viện Di Truyền Nông nghiệp
Study on the phenotypic variation and SSR markers/gene associated with seedling
vigour under submergence at the seedling stage of Vietnamese lowland rice germplasms
Abstract
In direct-seeded rice ecosystem, seedling vigour under submergence is one of the
most important traits for stable stand establishment. In this study, a set of 150 Vietnamese
lowland rice cultivars was collected for studying of the “elongation strategy” by using the test
tube bioassay screening method. LD (linkage disequilibrium) mapping method was applied to
analyze the correlation between marker genotype and phenotype. The results showed that 7
SSR markers were detected association at or 1% either 5% significant level with the seedling
vigour under submerged condition by ANOVA analysis. This trait is considered to be
controlled by polygenic systems such as a key enzyme in ethylene biosynthesis (OsACSs).
The results showed that expression level of OsACS gene family highly induced under
submergence by RT-PCR and Real-Time PCR/qPCR analysis. The expression of gene
OsACS1 might be a key gene responsible for rice seedling vigour under submergence at postgermination stage. Our results provided useful information for future breeding and genetic
study to improve seedling vigour under submergence that supports for direct-seeded rice
ecosystem in Vietnam.
Key words: genetic diversity, simple sequence repeat (SSR) markers, allele frequency,
seedling vigour, submergence stress, rice (Oryza sativa L.).