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ENERGY Physiology Function:worklive -grows(mitosis) -reproduce(meiosis+fertilization) -metabolism(photosynthesis and respiration) -inheritance(generation) Definition of Energy 2 Laws of Energy: 1.Conservation of Energy- energy is neither created nor destroyed, only transformed(1st law) *forms of energy: solar, potential, mechanical 2.Entropy- during the transformation of energy, some energy become disordered & becomes heat(2nd law) *heat: “disordered energy” Coupled Reactions and Chemical Bonds *”coupled”: with partner *coupled reactions= redox reactions 1.Reduction- gains electrons -creates chemical bond -stores energy in molecule -Photosynthesis(anabolic) 2.Oxidation-loses electrons -breaks chemical bonds -releases energy -Respiration *electron must go to or come from somewhere *no energy=dead(kaya malamig ung bangkay) Life processes are driven by energy Plants are dynamic metabolic systems. *1000 reactions/sec Processes can be: -energy consuming (endergonic) or -energy releasing (exergonic) and -catabolic (breakdown) or -anabolic (synthesis) *endergonic: energy must be supplied *exergonic: energy must be released The most common and important forms of cellular energy: 1.Chemical bonds (e.g. ATP and CH2O) 2.Electrons (redox reactions) 3.Electrochemical gradients *”gradients”:difference of something *A–P~P~P A–P~P+P Source of energy: Sun *can’t recycle energy because of 2nd law *energy flow: one direction only * only matters and/or chemicals can be recycled PHOTSYNTHESIS AND RESPIRATION ~PIC1 Overview: The process that Feeds the Biosphere Photosynthesis-process that converts solar energy to chemical Energy -occurs in plants, algae, certain other protists and some prokaryotes *no photosyntehsis, we won’t have food *cyanobacteria (dati tawag sa kanila blue green algae meron din sabi bacteria so nagging cyanobacteria) Plants and other autotrophs – producers of the biosphere Plants are photoautotrophs -they use the energy of sunlight to make organic molecules from water and carbon dioxide Heterotrophs-obtain their organic material from other Organisms -are the consumers of the biosphere *herbivores, carnivores, omnivores (consumers) Concept 1: Photosynthesis converts light energy to the chemical energy of food *Photosynthesis: CO2 + H2O GLUCOSE, SUGAR + O2 The leaves of plants-major sites of photosynthesis *some stems (herbaceous plants) and roots: site of photosynthesis Stomata ~PIC2&3 Chloroplasts-organelles in which photosynthesis occurs -contain thylakoids and grana *parasitic plants: not photosynthetic Photosynthesis is summarized as: 6 CO2 + 12 H2O + LIGHT ENERGY C6H12O6 + 6 O2 + 6 H2O Photosynthesis- generally a reduction process (may oxidized: water and reduced:CO2) Chloroplasts split water into Hydrogen and oxygen, incorporating the electrons of hydrogen into sugar molecules Photosynthesis is a redox process -Water is oxidized, carbon dioxide is reduced Photosynthesis consists of two processes -the light reactions (light dependent reaction) -the Calvin cycle (light independent reaction)(or Melvin cycle) ~PIC4 ~PIC6 Concept 2: The light reactions convert solar energy to the chemical energy of ATP and NADPH ATP Produced in 3 ways 1.Photophosphorylation-source of energy is sunlight -involving light and ATP 2.Substrate level phosphorylation-bigger chemicals can be split (ex. Glucose) 3.Oxidative phosphorylation-goping through different gradientbreakdown of molecule to ATP NADPH: NADP + H + e NADPH (in thylakoid and grana) (pag NADPH na, punta sa Stroma) *NADPH:vehicle lang Light-form of electromagnetic energy, which travels in Waves Wavelength-distance between the crests of waves -determine the type of electromagnetic energy Electromagnetic Spectrum-the entire range of electromagnetic energy or radiation *UV: more energy *shorter wavelength: more energy *longer wavelength: less energy *Impt in photoshynthesis: visible light(ROYGBIV) *light: absorbed or reflected *what you see: reflected light *Absorbed: all except what you see *White: reflected all colors, absorbed black *black: absorbed all colors, reflected none Pigments- substances that absorb visible light -light deceptors - reflect light, which include the colors we see *melanin:pigment in people, the more the darker skin *blue/red: maximum absorption of plants *chlorophyll A: mas bida *pag tinamaan ng light, absorb ng pigment esp. electron The absorption of spectra of three types of pigments in chloroplasts *the different experiments helped reveal which wavelengths of light are photosynthetically important *Result: ~PIC5 Chlorophyll A-main photosynthetic pigment Chlorophyll B- accessory pigment Other Accessory Pigments-absorb different wavelengths of light and pass the energy to chlorophyll A’ When a pigment absorbs light -it goes from a ground state to an excited state, which is Unstable *pag excited yung electron, may energy absorbed *Fluorescence: with small amount of light *excited state must release energy Photosystem- a reaction center associated with lightharvesting complexes -comnposed of a reaction center surrounded by a number of lightharvesting complexes -When a reaction-center chlorophyll molecule absorbs energy, one of its electrons gets bumped up to a primary electron acceptor -The light-harvesting complexes -consist of pigment molecules bound to particular Proteins -funnel the energy of photons of light to the reaction center ~PIC7 Noncyclic electron flow- primary patrhway of energy transformation in the light reactions -produces NADPH, ATP, O2 *pigment Molecules: magkukulang ng isang electron *pag kulang ng 1 electron, parang adik *most convenient source of e: water Water splits(through photolysis: breakdown using light) into electron(to regain lost e) Oxygen(ung hinihinga natin) and Hydrogen(for the reduction of ATP) *water breaks down in the presence of light *splitting of water: only in the presence of chlorophyll and light Light Reaction (NonCyclic Electron Flow) Reactants Products H2O O2(air) ADP + iP ATP(Calvin Cycle) NADP NADPH(Calvin Cycle) *iP= inorganic phosphate ~PIC8 Under certain conditions, photoexcited electrons take an alternative path. Cyclic electron flow- only photosystem I is used -only ATP is produced *electron will return to it original source *in some bacteria, only product: ATP ~PIC9 Concept 3: the calvin cycle uses ATP and NADPH to convert CO2 to sugar Calvin Cycle- similar to the citric acid cycle -Occurs in stroma *mas matipid(cycle in stroma: stroma reactions) *Melvin Calvin used paper chromatography and isotopes The Calvin cycle has three phases -Carbon Fixation: CO2 is fixed to an acceptor(RuBP) *RuBP: 5 carbon sugar * RuBP + CO2(from air) -Reduction -Regeneration of the CO2 acceptor ~PIC10 Calvin Cycle Reactants Products CO2 C6H12O6 / 3GP ATP ADP + iP NADPH NADP *C3 Photosynthesis: kc C3 first product *all plants have C3 Photosynthesis Concept 4: Atlernative mechanisms of carbon fixation have evolved in hot, arid climates *stomata open during day to allow gasses to enter but water evaporates On hot, dry days, plants close their stomata -conserving water but limiting access to CO2 -causing oxygen to build up *photorespiration: result of building up of oxygen Photorespiration – O2 substitutes for CO2 in the active site of enzyme rubisco -photosynthetic rate is reduced Rubisco- enzymes responsible for RuBP + CO2 Carbon *minsan nagkakamali ung rubisco so… RuBP + O2 photorespiration *masisira RuBP, will not continue Calvin cycle *disadvantage to plants so plants have ways to adapt Calvin Cycle: RuBP + CO2 3GP + 3GP (glucose) *RuBP = C5 Adaptations 1.C4 plants- minimize the cost of photorespiration by incorporating CO2 into four Carbon compounds in mesophyll cells *“C4” kasi initial product 4 C *These four carbon compounds are exported to bundle sheath cells, where they release CO2, used in Calvin Cycle ~PIC11 2.CAM Plants- open their stomata at night, incorporating CO2 into organic acids -“Crassulean Acid Metabolism” -looks like cactus -in hot areas -During the day, the stomata closes and the CO2 is released from organic acids for use in the Calvin Cycle *The CAM pathway is similar to C4 pathway ~PIC12 *all with Calvin Cycle, some CAM, some C4 *Carbon Dioxide- during day lang nagagamit kasi with sunlight for light reaction *No photosynthesis, no body of plants, no food/rice for us Organic compounds produced by photosynthesis -provide the energy and building material for ecosystems C3 CAM C4 Mechanism Mechanism Mechanism CO2 CO2 CO2 NADP NADP Reactants NADP ADP + iP ADP + iP ADP + iP H2O H2O NADPH NADPH NADPH ATP ATP ATP Products O2 O2 O2 C6H12O6 C6H12O6 C6H12O6 Initial CO2 PEP(at PEP(mesophyll) fixation night) RuBP RuBP(bundle Final CO2 RuBP(at sheath) Fixation daytime) Example Rice Sugarcane pineapple *NADPH, O2, ATP :produced in light *C6H12O6: produced in calvin *Glucose: w/ many energy but relatively large *Glucose to ATP(Cellular Respiration) Cellular Respiration Harvesting chemical energy So we see how energy enters food chains(via autotrophs) we can look at how organisms use that energy to fuel their bodies Plants and animals both use products of photosynthesis(glucose) for metabolic fuel Heterotrophs: must take in energy from outside sources, cannot make their own e.g. animals When we take in glucose (or other carbs) proteins, and fats-these foods don’t come to us the way our cells can use them Cellular Respiration overview Transformation of chemical energy in food into chemical energy cells can use:ATP These reactions proceed the same way in plants and animals. Process is called cellular respiration Overall reaction: C6H12O6 + 6 O2 6 CO2 + 6 H2O Cellular Respiration- chemical-bond energy in sugars is converted to energy-rich compound ATP which can then be used for metabolic reactions *break down of glucose *oxidation *lose electron, break bonds, release energyATP *Supposedly.. C6H12O6 + 6 O2 6 CO2 + 6 H2O + ATP * C6H12O6 + 6 O2 6 CO2 + 6 H2O + ATP (photosynthesis) *Photosynthesis is not the opposite of respiration *they only use each other’s products Stages of respiration: 1.Glycolysis -in cytoplasm -carbohydrates -breakdown *if O2 Absent, hanggang ditto nalang)(Anaerobic) *If O2 present…(Aerobic) 2. Krebs 3.ATC(Electron transport Chain) Breakdown of glucose begins in the cytoplasm: the liquid matrix inside the cell At this point life diverges into two forms and two pathways -Anaerobic cellular respiration (aka fermentation) -Aerobic cellular respiration Cellular respiration reactions Glycolysis -series of reactions which break the 6-carbon glucose molecule down into 3-carbonm molecules called pyruvate -process is an ancient one-all organisms from simple bacteria to humans perform it the same way -yields 2 ATP molecules for every one glucose molecule broken down -yields 2 NADH per glucose molecule Aerobic Cellular reaction Oxygen-required=aerobic 2 more sets of reactions which occur in a specialized structure within the cell called the mitochondria -1.krebs cycle -2.Electron Transport chain ~PIC13 and 14 Kreb’s cycle Completes the breakdown of glucose -takes the pyruvate (3-carbons) and breaks it down the carbon and oxygen atoms end up in CO2 and H2O -hydrogens and electrons are stripped and loaded onto NAD+ and FAD to produce NADH and FADH2 Production of only 2 more ATP but loads up the coenzymes with H+ and electrons which move to the 3rd stage *NADP + H + C NADPH*NAD + H + C NADH (respiration) *FAD + 2 h + 2 c FADH2 (respiration) Electron Transport Chain Electron carriers loaded with electrons and protons from the Kreb’s cycle move to this chain-like a series of steps(staircase) As electrons drop down stairs, energy released to form a total of 32 ATP Oxygen waits at bottom of staircase, picks up electrons and protons and in doing so becomes water Energy tally 36 ATP for aerobic vs. ATP for anaerobic -Glycolysis 2 ATP -Kreb’s 2 ATP -ETC 32 ATP Anaerobic organisms can’t be too energetic but are important for global recycling of carbon ~PIC15 *1 glucose= 36 ATP *each cell needs thousands of ATP Anaerobic cellular respiration Some organisms thrice in environments with little or no oxygen -marches, bogs, gut of animals, sewage treatment ponds No oxygen used=”an”aerobic Results in no more ATP, final steps in these pathways serve only to regenerate NAD+ so it can return to pick up more electrons and hydrogens in glycolysis End products such as ethanol and CO2 (single cell fungi(yeast) in beer/bread) or lactic acid(muscle cells) *Ethanol:small amount of ATP9only 21 so yeast will die) -15% yeast will die(kaya sealed wine) *fermentation product=yeast *happens in yeast and cheese *in animals, will feel cramps (due to presence or building up of lactic acid)(focus of oxygen brain nalang) *need CO2:photosyntehsis *need O2: cellular respiration *leaves: photosynthesis and respiration *roots: mainly respiration only *woody trees(stem):respiration *non-photosynthetic parts:respiration *hole in the bark:lenticel(entry of gasses) *O2:most important for respiration Reactants Products Stages Aerobic C6H12O6 + 6 O2 6 CO2 + 6 H2O =36 ATPS Glycolysis Krebs ETC Glycolysis Reactants C6H12O6 Products Pyruvate CO2 Anaerobic C6H12O6 Ethanol/lactic acid 2 atp CO2 glycolysis Kreb’s Pyruvate Acetyl COa NAD FAD NADH FADH2 CO2 ETC ADP + iP NADH FADH2 ATP H2O Reactants Products Events Where Photosynthesis Respiration CO2 + H2O C6H12O6 + 6O2 O2 6 CO2 glucose 2 H2O Glycolysis Light reaction Kreb’s cycle Calvin cycle ETC Mesophyll Mitochondria Bundle sheath cytoplasm chloroplast