Download Lecture Notes with Key Figures

Lecture Notes with Key Figures
PowerPoint® Presentation for
BROCK
BIOLOGY OF
MICROORGANISMS
ELEVENTH EDITION
MICHAEL T. MADIGAN
JOHN M. MARTINKO
CHAPTER 2
An Overview of Microbial Life
Copyright © 2006 Pearson Prentice Hall, Inc.
Chapter 2
An Overview of Microbial Life
PART I Cell Structure and Evolutionary History,
p. 22
2.1 Elements of Cell and Viral Structure, p. 22
2.2 Arrangement of DNA in Microbial Cells, p. 24
2.3 The Tree of Life, p. 26
PART II Microbial Diversity, p. 28
2.4 Physiological Diversity of Microorganisms, p.
28
2.5 Prokaryotic Diversity, p. 30
2.6 Eukaryotic Microorganisms, p. 35
PART I Cell Structure and
Evolutionary History, p. 22
2.1 Elements of Cell and Viral
Structure, p. 22
• All microbial cells share certain basic
structures in common, such as cytoplasm, a
cytoplasmic membrane, ribosomes, and
(usually) a cell wall.
• Two structural types of cells are recognized:
the prokaryote and the eukaryote.
Prokaryotic cells have a simpler internal
structure than eukaryotic cells, lacking
membrane-enclosed organelles (Figure 2.1).
• Viruses are not cells but depend on cells for
their replication (Figure 2.3c).
• Ribosomes—the cell's protein-synthesizing
factories—are particulate structures composed
of RNA (ribonucleic acid) and various
proteins suspended in the cytoplasm.
• Ribosomes interact with several cytoplasmic
proteins and messenger and transfer RNAs in
the key process of protein synthesis
(translation) (Figure 1.4).
2.2 Arrangement of DNA in
Microbial Cells, p. 24
• Genes govern the properties of cells, and a
cell's complement of genes is called its
genome. DNA is arranged in cells to form
chromosomes. In prokaryotes, there is
usually a single circular chromosome;
whereas in eukaryotes, several linear
chromosomes exist.
• Plasmids are circular extrachromosomal
genetic elements (DNA), nonessential for
growth, found in prokaryotes.
• The nucleus is a membrane-enclosed
structure that contains the chromosomes in
eukaryotic cells. The nucleoid, in contrast, is
the aggregated mass of DNA that constitutes
the chromosome of cells of Bacteria and
Archaea (Figure 2.4).
2.3 The Tree of Life, p. 26
• Comparative ribosomal RNA sequencing
has defined the three domains of life:
Bacteria, Archaea, and Eukarya.
• Molecular sequencing has also shown that
the major organelles of Eukarya have
evolutionary roots in the Bacteria and has
yielded new tools for microbial ecology and
clinical microbiology.
• Although species of Bacteria and Archaea
share a prokaryotic cell structure, they differ
dramatically in their evolutionary history.
• Evolution is the change in a line of descent
over time leading to new species or varieties.
The evolutionary relationships between life
forms are the subject of the science of
phylogeny.
• In addition to the genome in the
chromosomes of the nucleus, mitochondria
and chloroplasts of eukaryotes contain their
own genomes (DNA arranged in circular
fashion, as in Bacteria) and ribosomes.
• Using ribosomal RNA sequencing
technology (Figure 2.6), these organelles
have been shown to be highly derived
ancestors of specific lineages of Bacteria
(Figure 2.7).
• Mitochondria and chloroplasts were thus
once free-living cells that established stable
residency in cells of Eukarya eons ago. The
process by which this stable arrangement
developed is known as endosymbiosis.
PART II Microbial Diversity, p.
28
2.4 Physiological Diversity of
Microorganisms, p. 28
• All cells need carbon and energy sources.
Chemoorganotrophs obtain their energy
from the oxidation of organic compounds.
Chemolithotrophs obtain their energy from
the oxidation of inorganic compounds.
Phototrophs contain pigments that allow
them to use light as an energy source. (Figure
2.8)
• Autotrophs use carbon dioxide as their
carbon source, whereas heterotrophs use
organic carbon.
• Extremophiles thrive under environmental
conditions in which higher organisms cannot
survive. Table 2.1 gives classes and examples
of extremophiles.
2.5 Prokaryotic Diversity, p. 30
• Several lineages are present in the domains
Bacteria and Archaea, and an enormous
diversity of cell morphologies and
physiologies are represented there.
• Retrieval and analysis of ribosomal RNA
genes from cells in natural samples have
shown that many phylogenetically distinct but
as yet uncultured prokaryotes exist in nature.
• The Proteobacteria is the largest division
(called a phylum) of Bacteria (Figure 2.9).
• The Cyanobacteria (Figure 2.12) are
phylogenetic relatives of gram-positive
bacteria and are oxygenic phototrophs.
• There are two lineages of Archaea, the
Euryarchaeota and the Crenarchaeota (Figure
2.18).
2.6 Eukaryotic
Microorganisms, p. 35
• Microbial eukaryotes are a diverse group
that includes algae, protozoa, fungi, and slime
molds (Figure 2.22).
• Collectively, microbial eukaryotes are
known as the Protista. Some protists, such as
the algae, are phototrophic.
• Cells of algae and fungi have cell walls,
whereas the protozoa do not.
• Some algae and fungi have developed
mutualistic associations called lichens.