Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Monoclonal antibody wikipedia , lookup
Lymphopoiesis wikipedia , lookup
Molecular mimicry wikipedia , lookup
Adaptive immune system wikipedia , lookup
Cancer immunotherapy wikipedia , lookup
Innate immune system wikipedia , lookup
Polyclonal B cell response wikipedia , lookup
Psychoneuroimmunology wikipedia , lookup
Get reliable results in your T cell differentiation culture Polarizing TH cells from naive CD4+ T cells T helper cells – important subsets regulating immune responses stimulates immune responses and leads to antibody production of B cells, immunoglobulin class switch, and macrophage activation. Their crucial impact on immune responses and distinct role in disease, makes TH cells a focus of many researchers studying immune regulation. The various TH cell subsets can be differentiated from naive CD4+ T cells in vitro using specific combinations of cytokines (fig. 1). CD4+ T helper (TH) cells play a central role in the adaptive immune system by controlling a variety of cellular responses, defending against intra-vesicular agents (TH1) and external pathogens (TH2). Their cytokine secretion suppresses or Naive CD4 T cell IL-2* Anti-CD3 Anti-CD28 IL-4* IL-6* IL-31 IL-23 TSLP IL-25 Anti-IL-12 Anti-IFN-γ IL-12 IL-18 IL-27 IFN-γ Anti-IL-4 TH1 cell TH2 cell TGF-β1 IL-4* Anti-IFN-γ TH9 cell TGF-β1 IL-1β* IL-6* IL-21 IL-23 Anti-IL-2 Anti-IL-4 Anti-IFN-γ TGF-β1 iTreg cell TH17 cell IL-6* IL-21* CXCL13 Tfh cell IL-6* TNF-α* TH22 cell CD4, CD94, CD119 (IFN-γ), CD183 (CXCR3), CD195 (CCR5), Tim-3 CD4, CD119 (IFN-γ), CD193 (CCR3), CD194 (CCR4), Tim-1 CD4, TCRα/β CD196 (CCR6) CD4, CD25, CD127 CD4, CD161, CD194 (CCR4), CD196 (CCR6) CD4, TCRα/β, CD185 (CXCR5), CD197 (CCR7), CD69 CD4, CD194 (CCR4), CD196 (CCR6), CCR10 T-bet, STAT1, STAT4 GATA3, STAT6 IRF4, GATA3, STAT6 FoxP3, STAT5, Smad2 RORγt, STAT3 STAT3, Bcl6 BNC2, FOXO4 IL-2, IL-10, IFN-γ, TNF-α IL-3, IL-4, IL-5, IL-6, IL-10, IL-13 IL-9, IL-10 IL-10, TGF-β1, IL-35 IL-17A, IL-17F, IL-21, IL-22, IL-26 IL-6, IL-10, IL-12, IL-21 IL-13, IL-22, TNF-α Surface marker profile Transcription factors Polarizing cytokines Blocking or neutralizing antibodies Activating antibodies * also available in GMP grade Figure 1: Polarization of naive CD4 + T cells into TH cell subsets. Secreting cytokines Polarizing T helper cells from naive CD4+ T Cells Miltenyi Biotec provides the tools and support you need to differentiate T helper cells – from sample preparation, cell enrichment and cell culture, to analysis. • Eliminate variability in cell culture with premium MACS® Cytokines • Serum-free T cell culture medium for standardized cell culture conditions • Comprehensive technical support from the experts in T cells Standardize your cell culture Increase reproducibility in your polarization experiments with TexMACS™ Medium, an optimized serum-free T cell culture medium available in research and GMP grade. Get consistent cell growth and eliminate lot-to-lot variability with premium MACS Cytokines to ensure identical unit dosage. Start with gentle sample preparation Get high yields of viable cells with preserved epitopes for your downstream applications. The gentleMACS™ Dissociators with MACS Tissue Dissociation Kits enable gentle and effective dissociation of many tissue types, including tumor and skin. Figure : TexMACS Medium and MACS Cytokines enable consistent polarization of naive CD + T cells into TH subsets. Figure 2: The gentleMACS Family for standardized tissue dissociation or homogenization. Isolate highly pure naive CD4+ T cells In about 18 minutes, you can have pure, viable naive CD4+ T cells for high-quality results. Automate the process with the autoMACS® Pro Separator for greater convenience and reproducibility. Need to process multiple samples in parallel? Choose the MultiMACS™ Cell24 Separator Plus. Figure 3: The MultiMACS Cell24 Separator Plus and autoMACS Pro Separator enable automated multisample cell separation for reproducible results. Sensitive flow cytometry Detect and enrich viable cytokine-secreting cells without fixation of dead cells by using MACS Cytokine Secretion Assays. For fast cytokine detection, check out the Rapid Cytokine Inspector Kits. Also, detect multiple cytokines in a single sample with MACSplex Cytokine Kits and the MACSQuant® Analyzer 10. Our flow cytometer’s pre-installed Express Mode enables full automation of acquisition, gating strategy, and analysis. Figure : MACSplex Cytokine Kit analysis template using the MACSQuant Analyzer’s Express Mode configuration. See the whole solution for polarizing T helper cells from naive CD4+ T cells at Miltenyi Biotec GmbH | Phone +49 2204 8306-0 | Fax +49 2204 85197 | [email protected] | www.miltenyibiotec.com Miltenyi Biotec provides products and services worldwide. Visit www.miltenyibiotec.com/local to find your nearest Miltenyi Biotec contact. Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use. autoMACS, gentleMACS, MACS, MACSQuant, MultiMACS and TexMACS are either registered trademarks or trademarks of Miltenyi Biotec GmbH. Copyright © 2015 Miltenyi Biotec GmbH. All rights reserved. 130-107-541 miltenyibiotec.com/polarizing