Download T helper cell polarization_150305.indd

Get reliable results in your T cell differentiation culture
Polarizing TH cells from naive CD4+ T cells
T helper cells – important subsets
regulating immune responses
stimulates immune responses and leads to antibody production
of B cells, immunoglobulin class switch, and macrophage
activation. Their crucial impact on immune responses and
distinct role in disease, makes TH cells a focus of many
researchers studying immune regulation. The various TH cell
subsets can be differentiated from naive CD4+ T cells in vitro
using specific combinations of cytokines (fig. 1).
CD4+ T helper (TH) cells play a central role in the adaptive
immune system by controlling a variety of cellular responses,
defending against intra-vesicular agents (TH1) and external
pathogens (TH2). Their cytokine secretion suppresses or
Naive CD4 T cell
IL-2*
Anti-CD3
Anti-CD28
IL-4*
IL-6*
IL-31
IL-23
TSLP
IL-25
Anti-IL-12
Anti-IFN-γ
IL-12
IL-18
IL-27
IFN-γ
Anti-IL-4
TH1 cell
TH2 cell
TGF-β1
IL-4*
Anti-IFN-γ
TH9 cell
TGF-β1
IL-1β*
IL-6*
IL-21
IL-23
Anti-IL-2
Anti-IL-4
Anti-IFN-γ
TGF-β1
iTreg cell
TH17 cell
IL-6*
IL-21*
CXCL13
Tfh cell
IL-6*
TNF-α*
TH22 cell
CD4, CD94,
CD119 (IFN-γ),
CD183 (CXCR3),
CD195 (CCR5),
Tim-3
CD4,
CD119 (IFN-γ),
CD193 (CCR3),
CD194 (CCR4),
Tim-1
CD4, TCRα/β
CD196 (CCR6)
CD4, CD25,
CD127
CD4, CD161,
CD194 (CCR4),
CD196 (CCR6)
CD4, TCRα/β,
CD185 (CXCR5),
CD197 (CCR7),
CD69
CD4,
CD194 (CCR4),
CD196 (CCR6),
CCR10
T-bet, STAT1,
STAT4
GATA3, STAT6
IRF4, GATA3,
STAT6
FoxP3, STAT5,
Smad2
RORγt, STAT3
STAT3, Bcl6
BNC2, FOXO4
IL-2, IL-10,
IFN-γ, TNF-α
IL-3, IL-4, IL-5,
IL-6, IL-10,
IL-13
IL-9, IL-10
IL-10, TGF-β1,
IL-35
IL-17A, IL-17F,
IL-21, IL-22,
IL-26
IL-6, IL-10,
IL-12, IL-21
IL-13, IL-22,
TNF-α
Surface marker profile
Transcription factors
Polarizing cytokines
Blocking or neutralizing antibodies
Activating antibodies
* also available in GMP grade
Figure 1: Polarization of naive CD4 + T cells into TH cell subsets.
Secreting cytokines
Polarizing T helper cells from naive CD4+ T Cells
Miltenyi Biotec provides the tools and support you need
to differentiate T helper cells – from sample preparation,
cell enrichment and cell culture, to analysis.
• Eliminate variability in cell culture with premium
MACS® Cytokines
• Serum-free T cell culture medium for standardized
cell culture conditions
• Comprehensive technical support from the experts
in T cells
Standardize your cell culture
Increase reproducibility in your polarization experiments
with TexMACS™ Medium, an optimized serum-free T cell
culture medium available in research and GMP grade.
Get consistent cell growth and eliminate lot-to-lot
variability with premium MACS Cytokines to ensure
identical unit dosage.
Start with gentle sample preparation
Get high yields of viable cells with preserved epitopes
for your downstream applications. The gentleMACS™
Dissociators with MACS Tissue Dissociation Kits enable
gentle and effective dissociation of many tissue types,
including tumor and skin.
Figure : TexMACS Medium and MACS Cytokines enable consistent
polarization of naive CD + T cells into TH subsets.
Figure 2: The gentleMACS Family for standardized tissue dissociation
or homogenization.
Isolate highly pure naive CD4+ T cells
In about 18 minutes, you can have pure, viable naive CD4+
T cells for high-quality results. Automate the process with
the autoMACS® Pro Separator for greater convenience
and reproducibility. Need to process multiple samples in
parallel? Choose the MultiMACS™ Cell24 Separator Plus.
Figure 3: The MultiMACS Cell24 Separator Plus and autoMACS Pro
Separator enable automated multisample cell separation for
reproducible results.
Sensitive flow cytometry
Detect and enrich viable cytokine-secreting cells without
fixation of dead cells by using MACS Cytokine Secretion
Assays. For fast cytokine detection, check out the Rapid
Cytokine Inspector Kits. Also, detect multiple cytokines
in a single sample with MACSplex Cytokine Kits and
the MACSQuant® Analyzer 10. Our flow cytometer’s
pre-installed Express Mode enables full automation of
acquisition, gating strategy, and analysis.
Figure : MACSplex Cytokine Kit analysis template using the
MACSQuant Analyzer’s Express Mode configuration.
See the whole solution for polarizing T helper cells from
naive CD4+ T cells at
Miltenyi Biotec GmbH | Phone +49 2204 8306-0 | Fax +49 2204 85197 | [email protected] | www.miltenyibiotec.com
Miltenyi Biotec provides products and services worldwide. Visit www.miltenyibiotec.com/local to find your nearest Miltenyi Biotec contact.
Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use. autoMACS, gentleMACS, MACS,
MACSQuant, MultiMACS and TexMACS are either registered trademarks or trademarks of Miltenyi Biotec GmbH. Copyright © 2015 Miltenyi Biotec GmbH. All rights reserved.
130-107-541
miltenyibiotec.com/polarizing