Download Chow, Lu-Ping 周綠蘋 - 臺大基因體醫學研究中心

Chow, Lu-Ping 周 綠 蘋
蛋白 體暨蛋白 質功能核心 實驗 室
Proteomics and Protein Function Core Laboratory
所長 ，臺 大 醫學 院 生物化 學 暨分 子生 物學 研究 所
N T U R e s e a rc h Ce n te r Fo r M e d i c a l E xce l l e n ce
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EDUCATION AND POSITIONS HELD:
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B.D. Department of Botany, National Chung Hsing University, Taiwan, 1980
Master D. Institute of Plant Biology, National Taiwan University, Taiwan, 1983
Ph.D. Department of Pharmaceutical Sciences, Tokyo University of Science, Tokyo, 1996
Instructor, Institute of Biochemistry & Molecular Biology College of Medicine, National Taiwan University, 1987- 1996
Associate Professor, Institute of Biochemistry & Molecular Biology, College of Medicine, National Taiwan University, 1996- 2001
Vice director, Department of Medical Genetics, National Taiwan University Hospital, 2001- 2007
Professor, Institute of Biochemistry & Molecular Biology, College of Medicine, National Taiwan University, 2001- 2008
Professor and chairman, Institute of Biochemistry & Molecular Biology, College of Medicine, National Taiwan University, 2008/12- Present
HONORS:
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Biochemical J. reviewer
Proteomics reviewer
J. Proteome Research reviewer
FEBS J. reviewer
RESEARCH INTERESTS:
1. Bacterial virulence factors and cancer development
2. Host-pathogen interaction and cancer biomarkers discovery
3. Biomarkers of gastric diseases
4. Hepatocellular carcinoma (HCC)-related serum biomarkers
SELECTED PUBLICATIONS:
1. Chan CH, Ko CC, Chang JG, Chen SF, Wu MS, Lin JT, and Chow LP*. Subcellular and Functional Proteomic Analysis of the Cellular
Responses Induced by Helicobacter pylori. Molecular & Cellular Proteomics 2006, 5(4) :702–13.
2. Lin YF, Wu MS, Chang CC, Lin SW, Lin JT, Sun YJ, Chen DS, and Chow LP*. Comparative immunoproteomics of identification and
characterization of virulence factors from Helicobacter pylori related to gastric cancer. Molecular & Cellular Proteomics 2006,
5(8) :1484-96.
3. Lin YF, Chen CY, Tsai MH, Wu MS, Wang YC, Chuang EY, Lin JT, Yang PC, and Chow LP*. Duodenal ulcer-related antigens from
Helicobacter pylori: immunoproteome and protein microarray approaches. Molecular & Cellular Proteomic s 2007, 6(6) : 1018-26.
4. Chiu LL, Lee KL, Lin YF, Chu CY, Su SN, and Chow LP*. Secretome analysis of novel IgE-binding proteins from Penicillium citrinum.
Proteomics-clinical applications 2008, 2(1): 33-45.
5. Wu MS, Chow LP*, Lin JT, and Chiou SH. Proteomic identification of biomarkers related to Helicobacter pylori-associated
gastroduodenal disease: challenges and opportunities. J Gastroenterol Hepatol . 2008, 23(11) : 1657-61.
蛋白體暨蛋白質功能核心實驗室
Proteomics and Protein Function Core Laboratory
Proteomics, a subset of functional
genomics, is the study of the proteins expressed
by an organism. These studies link genome
sequence data and gene function. More than
200 proteomics facilities have been established
worldwide. As a leading academic institution
in Taiwan, National Taiwan University's core
of services can provide fast, high-sensitivity
identification of proteins by mass spectrometry
performed with two instruments. MALDI-TOF
(Matrix-assisted laser desorption/ionization
time-of-flight) mass spectrometry identifies
proteins by peptide mass fingerprinting. Liquid
chromatography-tandem mass spectrometry
(using Q-STAR) enables researchers to analyze
peptide sequence and post-translational
modification, using monosaccharide analysis,
oligosaccharide profiling, glycosylation sites and
detection of phosphorylated amino acids.
Furthermore, the facility offers consultation
and support for protein separation techniques,
proteome -related bioinformatics and data
management. Our ongoing projects are
designed to identify all the components of
cancer and infectious disease proteomes,
providing a basis for functional studies. We
will also address identification of functional
components of selected subcellular components.
The overarching goal of achieving new
understanding of diseases should yield important
new targets for therapeutic interpretation and
markers for early detection as well. The stated
mission of the Proteome Core Facility is to
provide comprehensive technological support
for biomedical research, both basic and with
potential clinical applications, on topics ranging
from cancer and pathogenesis of infectious
diseases to drug and vaccine design.
Our services include:
1. Two-dimensional electrophoresis (2-DE)
analysis (CBB, Silver Stain, Ruby, Cy3/Cy5
labling)
2. Protein digestion (In gel, In solution)
3. Identification of protein/peptide molecular
weight by mass spectrometry (MALDI-TOF)
4. Identification of peptide sequence and posttranslational modification (LC-MS/MS)
5. Prediction of unknown protein by mass
spectrometry database search with MASCOT
software (MALDI-TOF, LC-MS/MS)
│ Proteomics and Protein Function Core Laboratory
The completion of the genomes for a
large number of organisms has begun to
revolutionize experimental approaches to a
variety of problems in biology and medicine.
The proteome is of intense interest to
investigators because proteins are the major
functional components of the cell. In the era
of genomic research, characterization of a
protein links it directly to its corresponding
gene; understanding the function of a particular
protein leads directly to gene function.
Lu-Ping Chow, Ph.D
蛋白體暨蛋白質功能核心實驗室
教授 周綠蘋
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N T U R e s e a rc h Ce n te r Fo r M e d i c a l E xce l l e n ce
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Figure 1. Proteomics two-dimensional electrophoresis (2-DE) equipments
(A)
(B)
(D)
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2-d
(C)
(E)
Figure 2. Hardware equipments and core services. (A) Fluorescence Microscopy; (B) Akta FPLC
purification system; (C) Typhoon 9400 scanner; (D) MALDI-TOF mass spectrometry; (E) LC-MS/MS.
蛋白體暨蛋白質功能核心實驗室
Strategies for glycoprotein identification and characterization
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Figure 4. Strategies for glycoprotein identification and characterization.
│ Proteomics and Protein Function Core Laboratory
Figure 3. Strategy of protein profile alteration between normal and abnormal cells.
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N T U R e s e a rc h Ce n te r Fo r M e d i c a l E xce l l e n ce
申請服務流程
Cells (state B)
Cells (state A)
Heavy AA
Light AA
SILAC labeling
選擇特定的服務細項<二維電泳分析、
蛋白質水解分析、微量蛋白質定序(LCMS/MS)、基質輔助雷射脫附游離/飛行
時間質譜(MALDI-TOF MS)>下載其申請表
格，來電預約後，將填妥之申請表與檢
體一併親自送至蛋白質暨蛋白質功能核
心實驗室。
Tryptic digestion of cellular proteins
Phosphopeptide enrichment
初步核定分析樣品之品質與申請表格填
寫之完整度後，依收件順序進行測試。
LC-MS/MS
MASCOT analysis
Ingenuity Pathways Analysis
Figure 5. Strategies for phosphoprotein
identification and characterization.
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Contact information
信箱 : [email protected]
聯絡人: 周綠蘋
聯絡分機 : 88682