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Netherlands
Proteomics
Platform
FALL Meeting Netherlands Proteomics Platform
Friday Oct 13 2006, LUMC (Collegezaal 4), Leiden
Hot topics in proteomics: quantification and
phosphorylation
9.00-9.30
Registration
1. Quantitative proteomics
1.1 Label-free MS data production and analysis
9.30-10.00
Introduction LC-MS & GLP issues
 Nico van de Merbel (PharmaBioResearch). Quantitative determination of
biomarkers by LC-MS/MS in a regulated environment
10.00-10.30
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10.30-11.00
Elena Marchiori (VU). Introduction to data pre-processing and statistical
validation of multi-variate data sets generated by LC-MS
Coffee and tea
11.00-12.00
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Elwin Verheij, (TNO Kwaliteit van Leven) Automated processing of
comprehensive LC-MS data
Peter Horvatovich (RUG) Comprehensive data processing and multivariate
statistical analysis for liquid chromatography – mass spectrometry data
Alex Henneman (LUMC): Data preprocessing of LC/CE-MS datasets
Arjen Lommen (WUR): MetAlign as a tool for alignment of multiple
hyphenated mass spectrometry data sets
12.00-12.30
General Discussion
12.30-13.30
Lunch + Posters
1.2 Stable-isotope labeling
13.30-14.30
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Ka Wan Li (VU): Application of quantitative proteomics to drug addiction
research
Maarten Loos (VU): Multiplex iTRAQ data analysis
Wilma Dormeyer (UU) Differential analysis of plasma membrane proteins of
embryonic stem cells and embryonic stem cell-derived cardiomyocytes
Marco de Groot (UU) Anaerobic or aerobic Saccharomyces cerevisiae
chemostats: effect on the proteome
14.30-15.00
General Discussion
15.00-15.30
Coffee and tea
2. Phosphoproteomics
15.30-16.30
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Simone Lemeer (UU). Identification of phosphotyrosine signaling proteins in
the embryonic development of the Zebrafish, Danio Rerio
Iris Stulemeijer (WUR) Analysis of the phosphoproteome of tomato in
response to the plant pathogen Cladosporium fulvum.
Eef Dirksen (UU). Investigating dynamic interactions between nuclear
proteins and histones upon DNA damage induction using an immobilized
peptide approach
Oleg Klychnikov (VU) Proteomic analysis of protein complexes and
phosphorylation patterns in the synapse.
16.30-17.00
General Discussion
17.00..
Drinks