Download ULLA Summer School 2005

ULLA Summer School 2005
Themes and Courses
Theme 2 - Proteomics and analytical chemistry - Per Andrén
Course 2:1 Proteomics and Peptidomics
Course leader: P Andrén, R van Heijden
Last for
2 days
Level
Basic to Advanced
Instructors:Dr. Per Andrén, Uppsala University, Sweden; Dr. Rob van der Heijden, LACDR, Leiden; Dr. Andy
Wilderspin, School of Pharmacy, London
Description: Proteomics is the process of identification of all proteins expressed in a given cell or tissue. Sensitive and
accurate measurement and identification of complex mixtures of proteins and peptides can be made using highresolution protein separation techniques and identification by mass spectrometry. This allows an insight into the
expression of proteins in complex biological systems under a variety of circumstances. For example, a normal cell can
be compared with a diseased cell such as a tumour cell. The course will cover sample preparation and fractionation;
theoretical and practical aspects of protein purification, characterization and production; separation techniques such as
two-dimensional gel electrophoresis and multidimensional liquid chromatography; mass spectrometry methods such as
MALDI imaging, quantitative proteomics; bioinformatics; and different proteomics applications.
Objectives: The aim with the course is to introduce the students into the field of proteomics and peptidomics, protein
purification and characterization. The course is aimed at researchers with a background in biology and/or chemistry
who wish to gain an insight into the most up-to-date methodologies used in proteomics.
Prerequisites: Intermediate level of biology and chemistry.
Maximum numbers of students: 20
Course 2:2 Novel bioanalytical techniques
Course leader: D Westerlund.
Last for
1 day
Level
Maximum number of students: No limit
Instructors: Henk Lingeman, Douglas Westerlund
Description: Novel techniques and methods for the qualitative and quantitative analysis of drugs and biomolecules in
biological materials from sample preparation through separation and detection will be considered. The most powerful
sample preparation technique at the moment is solid-phase extraction (SPE) in all its forms. Recent developments in
SPE with respect to new sorbent materials (e.g., mixed-mode sorbents, restricted-access materials, molecular-imprinted
materials), new techniques (e.g., solid-phase micro-extraction, stir-bar extraction, solid-phase dynamic extraction), the
potential of at-line automation (e.g., robotic systems, micro-titre plate format), the combination in on-line coupled
systems (e.g., LC-LC, SPE-LC, SPE-CE) as well as the potential of in-line SPE techniques (e.g. SPE-CE) will be
discussed. The prospective of monolithic columns and electrodriven separation techniques will also be highlighted.
Possibilities and limitations of different detection techniques will be treated with a special emphasis on mass
spectrometry and the potential of bio-specific detection modes. Procedures for metabolic studies with a focus on
applications of LC-MS/MS will be treated. The possibilities to speed up the total analytical procedure, to clean samples,
to concentrate the analytes, to perform a phase transfer and to avoid incompatibilities between the sample treatment step
and the separation step, and the separation and the detection step, will be discussed.
Obectives: Students will learn about the state-of-the-art and current trends in bioanalytical methodologies. The
knowledge will be important for the development of methods in the areas of pharmacokinetics, metabolism,
pharmacology and toxicology in pre-clinical and clinical studies. Participants will acquire knowledge in order to select
suitable sample preparation, separation and detection techniques, with emphasis on mass spectrometry, for actual
bioanalytical problems.
Prerequisites: Basic knowledge on organic chemistry, analytical chemistry, metabolism, pharmacology, immunology
and biochemistry