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BIOGRAPHICAL SKETCH
Provide the following information for the Senior/key personnel and other significant contributors in the order
listed on Form Page 2.
Follow this format for each person. DO NOT EXCEED FOUR PAGES.
NAME
Ying Ge
POSITION TITLE
Assistant Professor
eRA COMMONS USER NAME (credential, e.g.,
agency login)
Yingge
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as
nursing, include postdoctoral training and residency training if applicable.)
INSTITUTION AND LOCATION
Peking University, Beijing, P.R. China
Cornell University, Ithaca, NY
DEGREE
(if applicable)
BS
PhD
MM/YY
FIELD OF STUDY
1997
Chemistry
2002
Chemistry
A. Personal Statement
My career goal is to redefine molecular mechanisms in heart failure and cardiac regeneration
through systems biology approaches and translate the bench discoveries to the clinic. My research is
highly interdisciplinary at the interface of chemistry, biology and medicine, based on my unique
training and diverse research experiences. I was trained as an analytical chemist and a biochemist at
Cornell University under the joint supervision of Fred McLafferty (a pioneer in mass spectrometry) and
Tadhg Begley (a renowned enzymologist) working on biological mass spectrometry (MS) and thiamin
biosynthesis. After graduate school, I explored a career in pharmaceutical industry where I have the
opportunity to interact with a diverse group of scientists in drug discovery and development.
Nevertheless, this industry experience convinced me that my ultimate interest is in academia where I
can have the freedom to explore my own research interests and mentor young generation of
scientists. At the University of Wisconsin-Madison first as an Assistant Scientist and now as an
Assistant Professor, I have developed a keen interest in myocardial biology/heart failure and
established a vibrant and externally funded research program in cardiovascular proteomics and
systems biology. By creatively integrating my expertise in chemistry/proteomics with cardiac
biology/medicine, I aim to develop fundamental principles that can provide transformative insights into
the understanding of cardiac diseases and regeneration, to identify new molecular targets for
diagnosis, and ultimately provide novel treatments for heart failure. It is my belief that in order to
make significant impact in molecular medicine, we need to combine technological advances with in
vitro and in vivo functional studies and bridge the silos between basic and translational/clinical
research.
In the past seven years as an independent investigator, I have published 43 papers and am the
corresponding author for 25 of them. I have been awarded a Scientist Development Grant by
American Heart Association and two NIH R01 grants. Moreover, I have been an ad hoc reviewer for
NIH and AHA, reviewer for NSF and other domestic and international grant agencies. I have trained or
co-mentored 4 graduate students, 3 post-doc fellows, 7 undergraduate students, 1 medical school
student, and 2 high school students. I am currently mentoring 3 graduate student, 5 post-doc fellows,
and 4 undergraduate students.
I am very passionate about education and find genuine fulfillment in inspiring young scientists. My
satisfaction comes when I see students develop critical thinking and problem solving ability and thrive
in their career development stages. In my lab, I aim to create a stimulating and nurturing research
environment to train the young generation of scientists from diverse backgrounds. I am mentoring
students from chemistry, biology and medical scientist graduate programs.
B. Positions and Honors
Positions and employment
1998-2002 Research Assistant, Department of Chemistry, Cornell University
2002-2003 Research Scientist III, Department of Chemical Technologies, Wyeth Research
2003-2004 Senior Research Scientist I, Department of Chemical Technologies, Wyeth Research
2004-2006 Research Scientist, Group Leader, Department of Analytical Development, PPD, Inc.
2006-present Director of Mass Spectrometry, Human Proteomics Program, School of Medicine and
Public Health, University of Wisconsin-Madison
2006-2011 Assistant Scientist, Department of Physiology, School of Medicine and Public Health,
University of Wisconsin-Madison
2011-2012 Assistant Scientist, Department of Cell and Regenerative Biology, School of Medicine
and Public Health, University of Wisconsin-Madison
2012-present Assistant Professor, Department of Cell and Regenerative Biology, School of Medicine
and Public Health, University of Wisconsin-Madison
Honors and professional activities
Awards: Shaw Scientist Finalist (2014); The Academy of Cardiovascular Research Excellence
Young Investigator Award (2011); Scientist Development Grant from the American Heart
Association Greater Midwest Affiliate (2007-2010); University of Kentucky Cardiovascular
Research Center Visiting Professor (2009); Canadian Society for Mass Spectrometry Student
Award (2001); North American FT-ICR Society Student Award (2001)
Reviewer for: NIH Study Section (Myocardial Ischemia and Metabolism) (2010, 2012, 2014); AHA
Cardiac Biology Regulation –Bsci6 Review Panel (2013, 2014); NIH Program Project Review
Panel (2014); Swiss Science Foundation (2012), NIH Special Emphasis Panel (Cardiovascular
and Respiratory Sciences) (2011); National Science Foundation Major Research Instrumentation
(MRI) Program (2011); Canada Foundation for Innovation Leaders Opportunity Fund; Pacific
Northwest National Laboratory EMSL Peer Review Panel (2009); Ontario Research Fund - Global
Leadership Round in Genomics & Life Sciences Competition (2009).
Reviewer for: Nature, PNAS, Molecular and Cellular Proteomics, Circulation Research, Circulation
Cardiovascular Genetics, Journal of the American Society for Mass Spectrometry, Journal of
Proteome Research, Proteomics, Proteomics-clinical Application, Analytical Chemistry,
International Journal of Mass Spectrometry, Chemical Research in Toxicology, Journal of Mass
Spectrometry, Journal of Muscle Research and Cell Motility, Journal of Cellular and Molecular
Cardiology, Journal of Chromatography A
Guest editor for a special issue on “Cardiovascular Proteomics in Clinical and Translational
Application” for Proteomics-clinical application (2014)
Conference organizer for: University of Wisconsin Human Proteomics Symposium (2006, 2007);
University of Wisconsin Proteomics workshop (2010); Wisconsin Human Proteomics Symposium
(2011, 2013); The Federation of Analytical Chemistry and Spectroscopy Society Annual Meeting,
Session on “Tandem MS Big & Small” (2013); American Society for Mass Spectrometry Annual
Conference, Workshop on Consortium of Top-down Proteomics (2013); American Society for
Mass Spectrometry Annual Conference, Oral Session on “Phosphoproteomics in Disease” (2014)
C. Selected Peer-reviewed Publications (57 publications total)
1. Peng, Y.; Gregorich Z. R.; Valeja, S. G.; Zhang, H.; Cai, W.; Chen, Y.; Guner, H.; Chen, A. J.;
Schwahn, D. J.; Hacker, T. A.; Liu, X.; Ge, Y. Top-down proteomics reveals concerted reductions
in myofilament and Z-disc protein phosphorylation after acute myocardial infarction, Mol. Cell.
Proteomics, 2014, Epub ahead of print. (Ge, Y. Corresponding author)
2. Xiu, L.; Valeja, S. G.; Alpert, A. J.; Jin, S.; Ge, Y. Effective protein separation by coupling
hydrophobic interaction and reverse phase chromatography for top-down proteomics, Anal.
Chem. 2014, Epub ahead of print. (Ge, Y. Corresponding author)
3. Peng, Y.; Chen, X.; Zhang, H.; Xu, Q.; Hacker, T. A.; Ge, Y. Top-down targeted proteomics for
deep sequencing of tropomyosin isoforms, J. Proteome Res. 2013, 12, 187-198. PMCID:
PMC3596867 (Ge, Y. Corresponding author)
4. Ge, Y.; Moss, R. L. Nitroxyl, redox switches, cardiac myofilaments, and heart failure: A prequel to
novel therapeutics? Circ. Res. 2012, 111, 954-956.
5. Dong, X.; Sumandea, C. A.; Chen, Y.; Garcia-Cazarin, M. L.; Zhang, J.; Balke, C. M.; Sumandea,
M. P.; Ge, Y. Augmented phosphorylation of cardiac troponin I in hypertensive heart failure, J.
Biol. Chem. 2012, 287, 848-857. PMCID:PMC3256890 (Ge, Y. Corresponding author)
6. Zhang, H.; Ge, Y. Comprehensive analysis of protein modifications by top-down mass
spectrometry, Circ. Cardiovasc. Genet. 2011, 4, 711. PMCID:PMC3320739 (Ge, Y.
Corresponding author)
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Zhang, J.; Guy, J. M.; Norman, H. A.; Chen, Y.; Dong, X.; Wang, S.; Kohmoto, T.; Young, K. H.;
Moss, R. L.; Ge, Y. Top-Down quantitative proteomics identified phosphorylation of cardiac
troponin I as a candidate biomarker for chronic heart failure, J. Proteome Res. 2011, 10, 40544065. PMCID:PMC3170873 (Ge, Y. Corresponding author)
Zhang, J.; Zhang, H.; Ayaz-Guner, S.; Chen, Y.; Dong, X.; Xu, Q.; Ge, Y. Phosphorylation, but
not alternative splicing or proteolytic degradation, is conserved in human and mouse cardiac
troponin T, Biochemistry, 2011, 50, 6081-6092. PMCID:PMC3170873 (Ge, Y. Corresponding
author)
Sancho Solis, R.; Ge, Y.; Walker, J. W. A preferred AMPK phosphorylation site in the inhibitory
loop of cardiac and skeletal troponin I, Protein Sci. 2011, 20, 894-907. PMCID: PMC3125873
(Ge, Y. Corresponding author)
Nelson, C. A.; Szezech, J. R.; Dooley, C. J.; Xu, Q.; Lawrence, M. J.; Zhu, R.; Jin, S.; Ge, Y.
Effective enrichment and mass spectrometry analysis of phosphopeptides using mesoporous
metal oxide nanomaterials, Anal. Chem. 2010, 82, 7193-7201. (Ge, Y. Corresponding author)
Zhang, J.; Dong, X.; Hacker, T. A.; Ge, Y. Deciphering modifications in swine cardiac troponin I
by Top-down high-resolution tandem mass spectrometry, J. Am. Soc. Mass. Spectrom. 2010,
21, 940-948. PMCID:PMC3056346 (Ge, Y. Corresponding author)
Ayaz-Guner, S.; Zhang, J.; Li, L.; Walker, J. W.; Ge, Y. In vivo phosphorylation site mapping in
mouse cardiac troponin I by high resolution top-down electron capture dissociation mass
spectrometry: Ser22/23 are the only sites basally phosphorylated, Biochemistry, 2009, 48, 81618170. (Ge, Y. Corresponding author)
Ge, Y.; Rybakova, I.; Xu, Q.; Moss, R. L. Top-down high resolution mass spectrometry of
cardiac myosin binding protein C revealed that truncation alters protein phosphorylation state,
Proc. Natl. Acad. Sci. U. S. A. 2009,106, 12658-12663. PMCID: PMC2722289 (Ge, Y.
Corresponding author) *This article is a PNAS Direct Submission (Track II).
Nelson, C. A.; Szczech, J. R.; Xu, Q.; Jin, S.; Ge, Y. Mesoporous Zirconium oxide nanomaterials
effectively enrich phosphopeptides for mass spectrometry-based phosphoproteomics, Chem.
Commun. 2009, 6607-6609. PMCID: PMC2790071
Zabrouskov, V.; Ge, Y.; Schwartz, J.; Walker, J. W. Unraveling molecular complexity of
phosphorylated human cardiac troponin I by top down electron capture dissociation/electron
transfer dissociation mass spectrometry, Mol. Cell. Proteomics 2008, 7, 1838-1849. (Ge, Y.
Corresponding author)
D. Research Support
Current
"Top-Down Proteomics of Myofilaments in Heart Failure"
Principal Investigator: Ge
Agency: NIH/NHLBI
Type: R01
Period: 8/5/2011 - 7/30/2016
Goals: To develop top-down mass spectrometry-based proteomics technologies for analysis of
key myofilament regulatory proteins and to understand the disease mechanism in left ventricular
hypertrophy and failure using a pressure overload animal model.
Impact/Priority Score: 18, Percentile: 8.0 (reviewed by CCHF study section)
"Deciphering Myofilament Modifications in Ischemic Cardiomyopathy"
Principal Investigator: Ge
Agency: NIH/NHLBI
Type: R01
Period: 3/1/2011 - 2/28/2017
Goals: To understand the molecular mechanism in ischemic cardiomyopathy and identify novel
targets for diagnosis and treatment of ischemic heart diseases through identification of ischemiainduced myofilament protein modifications.
Impact/Priority Score: 17, Percentile: 6.0 (reviewed by MIM study section)
“Nanotechnology Enabled Top-Down Mass Spectrometry-Based Phosphoproteomics”
Principal Investigator: Jin (Ge, co-PI)
Agency: NIH/NIBIB
Type: R21
Period: 2/01/2012-1/30/2014
Goals: To solve the challenge towards a comprehensive analysis of the human phosphoproteome
by developing a class of smart multivalent nanoparticle reagents for capturing phosphoproteins
globally out of human proteome followed by "top-down" proteomic analysis of intact
phosphoproteins.
Impact/Priority Score: 20, Percentile: 4.0 (reviewed by NANO study section)
“Bioenergetics in Hypertrophied and Remodeled Left Ventricle”
Principal Investigator: Zhang (University of Minnesota); (Ge, Co-I)
Agency: NIH/NHLBI
Type: R01
Period: 8/10/2012-5/31/2016 Goals: To determine the energetics in hypertrophied and remodeled
left ventricle towards a better understanding of the underlying mechanism in heart failure.
Impact/Priority Score: 11, Percentile: 3.0 (reviewed by CCHF study section)
Completed
“Deciphering Cardiac Troponin Modifications for Diagnosis of Heart Diseases”
Principal Investigator: Ge
Agency: American Heart Association Greater Midwest Affiliate
Type: Scientist Development Grant
Period: 07/1/07-6/30/11 (with no cost extension)
Goals: To gain a better understanding of the molecular mechanism in the development of heart
failure via characterization of modifications in cardiac troponin associated with heart diseases
using top down mass spectrometry based methodologies.