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By Jonathan Haltiwanger and
Teresa Leslie
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Analytical technique to determine the elemental
composition of a sample
Vaporization ionization electric field
mass-to-charge (m/z)
MS/MS- Tandem Mass Spectrometry
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Fragment specific ions to identify resulting fragment ions
Data is used to form structure
Can find specific compounds in complex mixture
http://upload.wikimedia.o
rg/wikipedia/commons/e
/eb/MS_MS.png
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Q- Quadrupole mass analyzer
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Filters sample ions (m/z)
TOF- Time-of-flight mass analyzer
Velocity depends on m/z
 q- Radio frequency collision
 B- Magnetic sector
 E- Electric sector
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http://www.ich.ucl.ac.uk/services_and_facilities/lab_services/mass_spectrometry/images/HPLC_ESI_MS_3.gif
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Precursor ion scan
Product ion scan
Neutral loss scan
Selected reaction monitoring
Fourier Transform Mass
Spectrometer (FTMS)
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Ionization issues
Ion suppression
Interfering compounds- “iso baric”
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Accurate Molecular Weight Measurements
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Reaction Monitoring
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Identification of protein using database “tag”
Oligonucleotide Sequencing
Post-Translation Modifications
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Monitor enzymes
Amino Acid Sequencing
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Sample confirmation, purity
Glycosylation, phosphorylation, disulfide
bridges
Protein Structure
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Protein-protein, protein-ligand, protein-DNA
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Screens for 30+ diseases
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Usually rare, inherited metabolic disorders
Measures acylcarnitine and amino acid
levels
Fast, accurate, cheap, and noninvasive
Star-G Project- Information on
disorders
Examples: Sickle cell disease,
phenylketonuria, maple syrup urine
disease
http://www.dshs.state.tx.us/lab/nbsHeelstick.jpg
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Difficulty breaking down phenylalanine
Enzyme phenylalanine hydroxylase absent
Leads to mental retardation, eczema,
seizures, and possibly death
http://www.bmb.leeds.ac.uk/illingworth/bioc1
010/image017.gif
http://media.wiley.com/CurrentProtocols/HG/h
g1701/hg1701-fig-0002-1-full.jpg
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Biotechnology
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Pharmaceutical
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Neonatal screening, hemoglobin analysis, drug
testing
Environmental
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Drug discovery, drug metabolism
Clinical
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Analysis of proteins, oligonucleotides
Water quality, food contamination
Geological
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Oil composition
http://www.astbury.leeds.ac.uk/facil/MStut/mstutorial.ht
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Mørtz E, O'Connor PB, Roepstorff P, Kelleher NL, Wood TD,
McLafferty FW, Mann M (1996). "Sequence tag identification of
intact proteins by matching tanden mass spectral data against
sequence data bases". Proc. Natl. Acad. Sci. U.S.A. 93 (16): 8264–7.
doi:10.1073/pnas.93.16.8264. PMID 8710858.
Sleno L, Volmer DA (2004). "Ion activation methods for tandem
mass spectrometry". Journal of mass spectrometry : JMS 39 (10):
1091–112. doi:10.1002/jms.703. PMID 15481084.
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Pictures from Wikipedia.com
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Other sources are already cited