Abstract_Metabolomic_RFMF
... By definition, metabolites are intermediates and products from different metabolisms. More commonly, they are small compounds (between 100 and 1000 Da) found in organisms that have an important part in cells live and survival. They can be identified with different technics such as Nuclear Magnetic R ...
... By definition, metabolites are intermediates and products from different metabolisms. More commonly, they are small compounds (between 100 and 1000 Da) found in organisms that have an important part in cells live and survival. They can be identified with different technics such as Nuclear Magnetic R ...
12866_2017_1009_MOESM5_ESM
... (twice, 20 sec for each time, at 7000 rpm) Mycelia lysate Centrifugation (5800 g, 10 min) Precipitate (Debris) ...
... (twice, 20 sec for each time, at 7000 rpm) Mycelia lysate Centrifugation (5800 g, 10 min) Precipitate (Debris) ...
Imaging Molecules using Ultra
... laser pulse (1013 W cm-2) is used to transfer a condensed phase sample into the vapor state for analysis. When femtosecond laser vaporization is combined with electrospray ionization mass spectrometry, a universal detector is produced that requires no sample work up and operates under ambient condit ...
... laser pulse (1013 W cm-2) is used to transfer a condensed phase sample into the vapor state for analysis. When femtosecond laser vaporization is combined with electrospray ionization mass spectrometry, a universal detector is produced that requires no sample work up and operates under ambient condit ...
Mass spectrometry
... Hans Dehmelt and Wolfgang Paul for the development of the ion trap technique in the 1950s and 1960s. • In 2002, the Nobel Prize in Chemistry was awarded to John Bennett Fenn for the development of electrospray ionization (ESI) and Koichi Tanaka for the development of soft laser desorption (SLD) and ...
... Hans Dehmelt and Wolfgang Paul for the development of the ion trap technique in the 1950s and 1960s. • In 2002, the Nobel Prize in Chemistry was awarded to John Bennett Fenn for the development of electrospray ionization (ESI) and Koichi Tanaka for the development of soft laser desorption (SLD) and ...
PDF
... to which an array of capture proteins is bound. The detection is usually performed with a specific probe which is labeled with a fluorescent dye following registration by a laser scanner [4]. Expression Proteomics is based on 2-dimensional (2-D) gel electrophoresis and mass spectrometry. 2-D gel ele ...
... to which an array of capture proteins is bound. The detection is usually performed with a specific probe which is labeled with a fluorescent dye following registration by a laser scanner [4]. Expression Proteomics is based on 2-dimensional (2-D) gel electrophoresis and mass spectrometry. 2-D gel ele ...
Structural Properties of Enzymes
... FAB-MS (Fast Atom Bombardment-Mass Spectrometry) is a relatively new technique in which the protein of interest in dissolved in glycerol, and bombarded with high energy Xe or Ar atoms. The gas-phase ions thus created are propelled in electrical and magnetic fields proportional to their charge and ma ...
... FAB-MS (Fast Atom Bombardment-Mass Spectrometry) is a relatively new technique in which the protein of interest in dissolved in glycerol, and bombarded with high energy Xe or Ar atoms. The gas-phase ions thus created are propelled in electrical and magnetic fields proportional to their charge and ma ...
Matrix-assisted Laser Desorption/Ionization Mass Spectrometry in
... Several theories have been developed to explain desorption of large molecules by MALDI. The thermal-spike model.6/ proposes that the matrix molecules sublime from the surface as a result of local heating at low laser fluence, but above a certain laser intensity, a rapid rise in desorption efficiency ...
... Several theories have been developed to explain desorption of large molecules by MALDI. The thermal-spike model.6/ proposes that the matrix molecules sublime from the surface as a result of local heating at low laser fluence, but above a certain laser intensity, a rapid rise in desorption efficiency ...
Protocol S3 – Proteomic analysis
... recombination using a selectable marker cassette essentially as previously described [1]. Briefly, cell-free soluble protein extracts were prepared by sonication of pelleted cells harvested from log-phase cultures grown in rich (LB) media using standard lysis buffer [1]. Half of each purified protei ...
... recombination using a selectable marker cassette essentially as previously described [1]. Briefly, cell-free soluble protein extracts were prepared by sonication of pelleted cells harvested from log-phase cultures grown in rich (LB) media using standard lysis buffer [1]. Half of each purified protei ...
field desorption (in mass spectrometry)
... decomposition. Most commercially available MALDI mass spectrometers now have a pulsed nitrogen laser of wavelength 337 nm. ...
... decomposition. Most commercially available MALDI mass spectrometers now have a pulsed nitrogen laser of wavelength 337 nm. ...
Mass spectrometry - Justin Benesch
... Mass-spectrometry based proteomics - Example 2 • Leptospira interrogans : pathogenic bacterium that causes leptospirosis • 83% of the proteome quantified, including proteins <10 copies per cell ...
... Mass-spectrometry based proteomics - Example 2 • Leptospira interrogans : pathogenic bacterium that causes leptospirosis • 83% of the proteome quantified, including proteins <10 copies per cell ...
Proteomics
... organelles, and then analyzing that portion. • This is because often proteins of interest, regulatory proteins are in low abundance. • The most commonly used method is 2dimensional gel electrophoresis. – Consists of using isoelectric focusing with SDS polyacrylamide gel electrophoresis ...
... organelles, and then analyzing that portion. • This is because often proteins of interest, regulatory proteins are in low abundance. • The most commonly used method is 2dimensional gel electrophoresis. – Consists of using isoelectric focusing with SDS polyacrylamide gel electrophoresis ...
Proteomics - OpenWetWare
... Step 1: Isolate cell or other protein source Step 2: Lyse cells and isolate proteins Step 3: Break up proteins into smaller (but still relatively large) amino acid chains Step 4: Separate chains (2D gel, gas or liquid chromatography) Step 5: Analyze separated protein parts by mass spectrometry ...
... Step 1: Isolate cell or other protein source Step 2: Lyse cells and isolate proteins Step 3: Break up proteins into smaller (but still relatively large) amino acid chains Step 4: Separate chains (2D gel, gas or liquid chromatography) Step 5: Analyze separated protein parts by mass spectrometry ...
An_explanation_of_the_pH_scale
... A base is fundamentals foundation or a main ingredient. It is the starting place and acts upon the acid. A base is the compound that reacts with the acid to form a salt because the molecules (ions) are able to take a proton from the acid and share a pair of electrons with the acid. (A salt consists ...
... A base is fundamentals foundation or a main ingredient. It is the starting place and acts upon the acid. A base is the compound that reacts with the acid to form a salt because the molecules (ions) are able to take a proton from the acid and share a pair of electrons with the acid. (A salt consists ...
Supplemental Methods
... source. The recovered peptides for ApoA-I, ApoB, ApoE, ApoC1, ApoC2, ApoC3, and PCSK9 were resolved by reverse-phase chromatography on a 1 x 50 mm Acquity UPLC BEH column packed with 1.7 µm C18 particles (Waters) that was maintained at 50ºC. Sample injection volumes varied from 2-8 µL. Peptides were ...
... source. The recovered peptides for ApoA-I, ApoB, ApoE, ApoC1, ApoC2, ApoC3, and PCSK9 were resolved by reverse-phase chromatography on a 1 x 50 mm Acquity UPLC BEH column packed with 1.7 µm C18 particles (Waters) that was maintained at 50ºC. Sample injection volumes varied from 2-8 µL. Peptides were ...
Attomole Detection of Proteins in a Complex Mixture Using the
... Low abundance proteins are often of biological interest and as such, sensitivity and low limits of quantification are key parameters in modern proteomic experiments. The SYNAPT® G2-S System provides improved sensitivity, resulting in the routine detection of attomole levels of tryptically-digested p ...
... Low abundance proteins are often of biological interest and as such, sensitivity and low limits of quantification are key parameters in modern proteomic experiments. The SYNAPT® G2-S System provides improved sensitivity, resulting in the routine detection of attomole levels of tryptically-digested p ...
Diseases of the immune system
... highthroughput mode. ELSD is sensitive to the mass of an analyte, rather than its absorbance or ionization efficiency . The advantage is that a more uniform response is obtained from small-molecule libraries with ELSD compared with UV absorbance. ELSD suffers from the limitation of not being abl ...
... highthroughput mode. ELSD is sensitive to the mass of an analyte, rather than its absorbance or ionization efficiency . The advantage is that a more uniform response is obtained from small-molecule libraries with ELSD compared with UV absorbance. ELSD suffers from the limitation of not being abl ...
problem set: atomic structure
... a) The electron affinity of Fluorine is more negative than Oxygen. b) The electron affinity of Magnesium is a positive value. c) The electron affinity for Phosphorus is less negative than Silicon. d) The electron affinity of Neon is a positive value. 4) a) Describe what you would see if you added: i ...
... a) The electron affinity of Fluorine is more negative than Oxygen. b) The electron affinity of Magnesium is a positive value. c) The electron affinity for Phosphorus is less negative than Silicon. d) The electron affinity of Neon is a positive value. 4) a) Describe what you would see if you added: i ...
Taícia P. Fill1, Edson Rodrigues Filho1
... formula C24H26N2O6. Sodium adduct ([M+Na]+) was also detected at m/z 461. The MS spectrum obtained from the extract of the experiment (13C-Phe added) contains the peaks at m/z 441 and 463 in addition to 439 and 461, previously ascribed to [M+H]+ and [M+Na]+ of standard brasiliamide A. These two unit ...
... formula C24H26N2O6. Sodium adduct ([M+Na]+) was also detected at m/z 461. The MS spectrum obtained from the extract of the experiment (13C-Phe added) contains the peaks at m/z 441 and 463 in addition to 439 and 461, previously ascribed to [M+H]+ and [M+Na]+ of standard brasiliamide A. These two unit ...
Chapter 2: Target/decoy search strategy for increased
... derived from set numbers of correct and incorrect PSMs. This program exploited the target-decoy principle and therefore relied on the same assumptions explained previously, namely that all decoy hits are incorrect, and that there are equal numbers of incorrect target and decoy hits. The program too ...
... derived from set numbers of correct and incorrect PSMs. This program exploited the target-decoy principle and therefore relied on the same assumptions explained previously, namely that all decoy hits are incorrect, and that there are equal numbers of incorrect target and decoy hits. The program too ...
Amino Acid Single and Three letter codes Name of Amino Acid
... can be made? Fmoc solid-phase synthesis What level of purification is necessary? What analysis can be done? ...
... can be made? Fmoc solid-phase synthesis What level of purification is necessary? What analysis can be done? ...
MALDI Imaging of Formalin-Fixed Paraffin
... of FFPE showed positive results on blocks stored for less than 2 days. However, most samples are stored for more than one year, and thus our objective was to establish a novel strategy using as a model system 6-hydroxydopamine (6-OHDA) treated rat brain tissues stored in FFPE blocks for more than 9 ...
... of FFPE showed positive results on blocks stored for less than 2 days. However, most samples are stored for more than one year, and thus our objective was to establish a novel strategy using as a model system 6-hydroxydopamine (6-OHDA) treated rat brain tissues stored in FFPE blocks for more than 9 ...
Matrix-assisted laser desorption/ionization
Matrix-assisted laser desorption/ionization (MALDI) is a soft ionization technique used in mass spectrometry, allowing the analysis of biomolecules (biopolymers such as DNA, proteins, peptides and sugars) and large organic molecules (such as polymers, dendrimers and other macromolecules), which tend to be fragile and fragment when ionized by more conventional ionization methods. It is similar in character to electrospray ionization (ESI) in that both techniques are relatively soft ways of obtaining ions of large molecules in the gas phase, though MALDI produces far fewer multiply charged ions.MALDI methodology is a three-step process. First, the sample is mixed with a suitable matrix material and applied to a metal plate. Second, a pulsed laser irradiates the sample, triggering ablation and desorption of the sample and matrix material. Finally, the analyte molecules are ionized by being protonated or deprotonated in the hot plume of ablated gases, and can then be accelerated into whichever mass spectrometer is used to analyse them.
