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... described previously [9]. The IPs were automatically summed up with their substrates during the MALDI-TOF scoring of peptide libraries, since their masses were identical, but in the HPLC analysis we were able to quantify the IPs as well, using Equations 1, 2 and 3. Eq. 4 was used in cases when the i ...
... described previously [9]. The IPs were automatically summed up with their substrates during the MALDI-TOF scoring of peptide libraries, since their masses were identical, but in the HPLC analysis we were able to quantify the IPs as well, using Equations 1, 2 and 3. Eq. 4 was used in cases when the i ...
Propionic Acid Degradation by Syntrophic Bacteria During
... to maintain continuous and efficient biogas production. The real reasons for this are not yet fully described and as cultivation of propionate degraders is difficult, the knowledge about syntrophic organisms capable of anaerobic oxidation of propionic acid is restricted. Research allowing better underst ...
... to maintain continuous and efficient biogas production. The real reasons for this are not yet fully described and as cultivation of propionate degraders is difficult, the knowledge about syntrophic organisms capable of anaerobic oxidation of propionic acid is restricted. Research allowing better underst ...
Article The Evolution of Momordica Cyclic Peptides
... Precursors of Momordica Cyclic and Acyclic Peptides Polymerase chain reaction (PCR) of Momordica genomic DNA using the primers that amplified TIPTOP genes from Momordica cochinchinensis and M. sphaeroidea (Mylne et al. 2012) resulted in new TIPTOP genes from two Asian Momordica, that is, one from M. ...
... Precursors of Momordica Cyclic and Acyclic Peptides Polymerase chain reaction (PCR) of Momordica genomic DNA using the primers that amplified TIPTOP genes from Momordica cochinchinensis and M. sphaeroidea (Mylne et al. 2012) resulted in new TIPTOP genes from two Asian Momordica, that is, one from M. ...
Full-Text PDF
... 2. Mass Spectrometric (MS) Techniques for Analysis of Ricin 2.1. Determination of Molecular Weight of the Intact Ricin Protein by Mass Spectrometric Analysis The simplest technique for analysis of ricin by mass spectrometry is to detect the intact ricin protein with an approximate molecular weight o ...
... 2. Mass Spectrometric (MS) Techniques for Analysis of Ricin 2.1. Determination of Molecular Weight of the Intact Ricin Protein by Mass Spectrometric Analysis The simplest technique for analysis of ricin by mass spectrometry is to detect the intact ricin protein with an approximate molecular weight o ...
PDF Links - Journal of the Korean Ceramic Society
... with the samples. The corresponding (201) peak intensity was reduced with the addition of Asn or Gly, indicating the decrease of crystal development. It may be said that the addition of Asn or Gly contributed to enhancing the organic-inorganic interaction between HAp and the GEL through the modifica ...
... with the samples. The corresponding (201) peak intensity was reduced with the addition of Asn or Gly, indicating the decrease of crystal development. It may be said that the addition of Asn or Gly contributed to enhancing the organic-inorganic interaction between HAp and the GEL through the modifica ...
The Amino-acid Sequence in the Phenylalanyl Chain of Insulin
... slow-moving peptides it was necessary to run the solvent considerably farther than the end of the filter paper. This was accomplished by attaching a second folded sheet of filter paper to the front of the chromatogram. Where this was done the number of lengths of filter paper through which the solve ...
... slow-moving peptides it was necessary to run the solvent considerably farther than the end of the filter paper. This was accomplished by attaching a second folded sheet of filter paper to the front of the chromatogram. Where this was done the number of lengths of filter paper through which the solve ...
The Amino Acid Sequences of the Fd Fragments of Two Human y1
... tion of the tryptic and chymotryptic peptides are The sequence of fragment 5a is complete as shown shown in Table 4. The sequence of fragment 5a in Fig. 2. Fragment 6a. This was digested with trypsin was determined, as shown in Fig. 2. Tryptic peptide T4.a was digested with chymotrypsin and (100lg/, ...
... tion of the tryptic and chymotryptic peptides are The sequence of fragment 5a is complete as shown shown in Table 4. The sequence of fragment 5a in Fig. 2. Fragment 6a. This was digested with trypsin was determined, as shown in Fig. 2. Tryptic peptide T4.a was digested with chymotrypsin and (100lg/, ...
1. dia
... Ordering of piperidine might need allowance, because it is the starting material in the synthesis of morphine. ...
... Ordering of piperidine might need allowance, because it is the starting material in the synthesis of morphine. ...
Mutational analysis of sickle haemoglobin (Hb
... this interaction. After this initial event, other intertetrameric lateral and longitudinal interactions strengthen the aggregate. The locations of many of these interactions have been identified by several methods, including procedures that utilize the sparing effect of other mutant Hb on the gelati ...
... this interaction. After this initial event, other intertetrameric lateral and longitudinal interactions strengthen the aggregate. The locations of many of these interactions have been identified by several methods, including procedures that utilize the sparing effect of other mutant Hb on the gelati ...
Protein Sequencing and Identification With Mass
... An Introduction to Bioinformatics Algorithms ...
... An Introduction to Bioinformatics Algorithms ...
Final Thesis Solid-phase bio-organic synthesis to create intelligent surfaces Patrik Nygren
... This thesis uses solid-phase synthesis to synthesize molecules that will arrange themselves as monolayers. The use of solid-phase synthesis will hopefully increase the yields in every step and also make it easier to synthesize more complex molecules. There are a vast number of different surfaces tha ...
... This thesis uses solid-phase synthesis to synthesize molecules that will arrange themselves as monolayers. The use of solid-phase synthesis will hopefully increase the yields in every step and also make it easier to synthesize more complex molecules. There are a vast number of different surfaces tha ...
The Primary Structure of the Calcium Ion
... The isolation and characterization of the soluble peptides from the CNBr digest of the calcium ion-transporting adenosine triphosphatase protein of rabbit skeletal sarcoplasmic reticulum are described. The 562 unique residues of the protein were placed in sequences. The remaining part of the protein ...
... The isolation and characterization of the soluble peptides from the CNBr digest of the calcium ion-transporting adenosine triphosphatase protein of rabbit skeletal sarcoplasmic reticulum are described. The 562 unique residues of the protein were placed in sequences. The remaining part of the protein ...
A Chemical Approach To Illustrate the Principal of Signal
... In nature, cellular functions are propagated by cascades of molecules, which interact with one another for signal transduction. Generally, the sequential process is initiated by the binding of an extracellular signal to a receptor culminating in one or more specific cellular responses In this way, a ...
... In nature, cellular functions are propagated by cascades of molecules, which interact with one another for signal transduction. Generally, the sequential process is initiated by the binding of an extracellular signal to a receptor culminating in one or more specific cellular responses In this way, a ...
Biosignature storage in sulfate minerals
... This dissertation represents the final research work for my Ph.D conducted from 2004 – 2009 at the University of Montana Geosciences Department. What follows are five chapters and two appendices. Chapter 1 provides a general introduction to the subjects covered within the research chapters including ...
... This dissertation represents the final research work for my Ph.D conducted from 2004 – 2009 at the University of Montana Geosciences Department. What follows are five chapters and two appendices. Chapter 1 provides a general introduction to the subjects covered within the research chapters including ...
Purification and Some: Characteristics of a Monomeric Racemase
... was compared with the relative mobility of protein standards (phosphorylase b, M, 94,000; bovine serum albumin, M, 67,000; ovalbumin, M, 43,000; carbonic anhydrase, M, 30,000; soybean trypsin inhibitor, Mr 20,100; rw-lactalbumin, Mr 14,400) to estimate the subunit molecular weight of the enzyme. Mat ...
... was compared with the relative mobility of protein standards (phosphorylase b, M, 94,000; bovine serum albumin, M, 67,000; ovalbumin, M, 43,000; carbonic anhydrase, M, 30,000; soybean trypsin inhibitor, Mr 20,100; rw-lactalbumin, Mr 14,400) to estimate the subunit molecular weight of the enzyme. Mat ...
T. Takahashi, B. C. Vo Ngo, L. Xiao, G. Arya, and M. J. Heller
... of Peptide 14 obtained during one of the simulations, with labeled Cys-His and end-to-end distances. Figure 6b shows a plot of the Cys-His distance vs. time over a 60 ns simulation run. Note that we performed five such simulations per peptide. The two main differences between our model peptides and t ...
... of Peptide 14 obtained during one of the simulations, with labeled Cys-His and end-to-end distances. Figure 6b shows a plot of the Cys-His distance vs. time over a 60 ns simulation run. Note that we performed five such simulations per peptide. The two main differences between our model peptides and t ...
Cleavage, Deprotection and Isolation of Peptides after Fmoc Synthesis
... describe a variety of cleavage and deprotection methods for peptides synthesized with an Fmoc/tBu ...
... describe a variety of cleavage and deprotection methods for peptides synthesized with an Fmoc/tBu ...
Amino Acid Sequences Containing Cysteine or Cystine Residues in
... Preparation of ovalbumin from turkey eggs commenced within 12 h of laying of the eggs. Crystallization by the method of Warner (1954) of ovalbumin from the isolated egg white was unsuccessful. The egg-white solution containing ovalbumin was recovered after the second precipitation with ammonium sulf ...
... Preparation of ovalbumin from turkey eggs commenced within 12 h of laying of the eggs. Crystallization by the method of Warner (1954) of ovalbumin from the isolated egg white was unsuccessful. The egg-white solution containing ovalbumin was recovered after the second precipitation with ammonium sulf ...
Amino Acid Sequences containing Cysteine or Cystine Residues in
... carboxymethy1cellulose columns, and is known to have a high cystine content. No stringent repurification was applied to the samples used for sequence work and no peptide amino acid sequences were found in recovered peptides, other than those that related to the sequences given in this paper. For goo ...
... carboxymethy1cellulose columns, and is known to have a high cystine content. No stringent repurification was applied to the samples used for sequence work and no peptide amino acid sequences were found in recovered peptides, other than those that related to the sequences given in this paper. For goo ...
Influence of free linoleic acid on the fatty acids profile of fermentation
... been reported. The dietary sources of CLA are milk fat, natural and processed cheeses, meat products and plant oils. Animal sources are richer in CLA than are plant sources [8]. Basically CLA can be produced by chemical synthesis or biosynthesis. Synthetic CLA is synthesized from linoleic acid by al ...
... been reported. The dietary sources of CLA are milk fat, natural and processed cheeses, meat products and plant oils. Animal sources are richer in CLA than are plant sources [8]. Basically CLA can be produced by chemical synthesis or biosynthesis. Synthetic CLA is synthesized from linoleic acid by al ...
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... Silica gel column chromatography Paper chromatography of silica gel chromatography fractions Thin layer chromatography of the trans-methyiglutaconate shunt intermediates Determination of alcohol dehydrogenase activity in the postmitochondrial supernatant Isolation and characterization of proteins an ...
... Silica gel column chromatography Paper chromatography of silica gel chromatography fractions Thin layer chromatography of the trans-methyiglutaconate shunt intermediates Determination of alcohol dehydrogenase activity in the postmitochondrial supernatant Isolation and characterization of proteins an ...
Amino Acid Sequences of Peptides from a Tryptic Digest of a Urea
... separated into 32 peptide fractions by cation-exchange resin chromatography. 2. Most of these fractions have been resolved into their component peptides by a combination of the techniques of eation-exchange resin chromatography, paper chromatography and paper electrophoresis. 3. The amino acid compo ...
... separated into 32 peptide fractions by cation-exchange resin chromatography. 2. Most of these fractions have been resolved into their component peptides by a combination of the techniques of eation-exchange resin chromatography, paper chromatography and paper electrophoresis. 3. The amino acid compo ...
working with peptides
... vortexing after every drop until the peptide dissolves. The drop wise addition of the organic solvent can also be used for peptides that do not respond to pH adjustment. Peptides that are >75% hydrophobic are unlikely to dissolve in aqueous solution alone and may require solubilization in a stronger ...
... vortexing after every drop until the peptide dissolves. The drop wise addition of the organic solvent can also be used for peptides that do not respond to pH adjustment. Peptides that are >75% hydrophobic are unlikely to dissolve in aqueous solution alone and may require solubilization in a stronger ...
CHAPTER 4 HF` Cleavage and Deprotection Procedures for
... cyclohexylamine) in dimethylformamide (20 mL/g of resin). 2. Stir or mix this suspension for 1 h at room temperature. 3. Filter on fritted-glass funnel, and wash resin with dimethyl formamide, followed by isopropanol, followed by dichloromethane. 4. Dry the resin overmght in a vacuum desiccator. Whe ...
... cyclohexylamine) in dimethylformamide (20 mL/g of resin). 2. Stir or mix this suspension for 1 h at room temperature. 3. Filter on fritted-glass funnel, and wash resin with dimethyl formamide, followed by isopropanol, followed by dichloromethane. 4. Dry the resin overmght in a vacuum desiccator. Whe ...
Matrix-assisted laser desorption/ionization
Matrix-assisted laser desorption/ionization (MALDI) is a soft ionization technique used in mass spectrometry, allowing the analysis of biomolecules (biopolymers such as DNA, proteins, peptides and sugars) and large organic molecules (such as polymers, dendrimers and other macromolecules), which tend to be fragile and fragment when ionized by more conventional ionization methods. It is similar in character to electrospray ionization (ESI) in that both techniques are relatively soft ways of obtaining ions of large molecules in the gas phase, though MALDI produces far fewer multiply charged ions.MALDI methodology is a three-step process. First, the sample is mixed with a suitable matrix material and applied to a metal plate. Second, a pulsed laser irradiates the sample, triggering ablation and desorption of the sample and matrix material. Finally, the analyte molecules are ionized by being protonated or deprotonated in the hot plume of ablated gases, and can then be accelerated into whichever mass spectrometer is used to analyse them.