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Analysis of Immune Deviation Elicited by Antigens Injected into the Subretinal Space Hartmut Wenkel andj. Wayne Streilein determine whether the subretinal space supports the induction of deviant immune responses to cell-associated and soluble antigens and to elucidate factors influencing the immunologic properties of the subretinal space. PURPOSE. TO P815 mastocytoma cells were used as cell-associated antigens and were inoculated into the anterior chamber (AC), the vitreous cavity (VC), the subretinal space, and subconjunctivally in H2-compatible, but minor H-incompatible, BALB/c mice. Ovalbumin, as a soluble antigen, was similarly injected into eyes, after which recipient animals were immunized with ovalbumin and complete Freund's adjuvant. Delayed-type hypersensitivity (DTH) was assessed by ear challenge. To alter the conditions in the subretinal space, the outer blood-retinal barrier was disrupted by compromising retinal pigment epithelial (RPE) cells with a systemic injection of sodium iodate. Immune privilege in the AC was abolished by mild corneal cauterization. METHODS. Antigen-specific DTH did not develop in mice in which alloantigenic tumor cells or ovalbumin was injected into the AC, the VC, or the subretinal space, and the mice's spleens contained lymphocytes capable of suppressing DTH when adoptively transferred into naive mice. When RPE cells were compromised with sodium iodate, tumor cells or ovalbumin injected into the subretinal space or the VC did not induce immune deviation, although the AC of these eyes still promoted AC-associated immune deviation. By contrast, when immune privilege in the AC was abolished by corneal cauterization, neither tumor cells nor ovalbumin injected into the subretinal space or the VC of eyes elicited immune deviation. RESULTS. The subretinal space supports immune deviation for histoincompatible tumor cells and soluble protein antigens by actively suppressing antigen-specific DTH. Acute loss of immune privilege in the subretinal space and the VC does not cause loss of privilege in the AC, but abolition of immune privilege in the AC eliminates the capacity of the subretinal space and the VC to support immune deviation to antigens injected locally. (Invest Ophthalmol Vis Set. 1998;39:1823-1834) CONCLUSIONS. A deviant immune response to cell-associated alloantigens and soluble antigens injected into the anterior chamber (AC) has been described in detail in the literature.1"7 The main characteristics of anterior chamber-associated immune deviation (ACAID) are impaired delayed-type hypersensitivity (DTH), T cells that suppress DTH, and the inability to produce complement-fixing antibodies.1 Antigenspecific immune deviation can be adoptively transferred into naive animals by an intravenous injection of splenocytes derived from mice that have had antigen injected into the AC. Splenic regulatory T cells that are responsible for immune deviation bear Thy 1.2 and CD8. A minority of these cells also express CD4 surface marker.3 So far, only a small number of stvidies have been reported that involve immune deviation elicited by antigens injected into the posterior part of the eye. 89 Jiang and Streilein showed From the Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts. Supported in part by Grant WE 1462/3-1 from Deutsche Forschungsgemeinschaft and by Grant EY 09595 from the United States Public Health Service, Washington, DC. Submitted for publication January 22, 1998; revised May 5, 1998; accepted June 4, 1998. Proprietary interest category: N. Reprint requests: J. Wayne Streilein, The Schepens Eye Research Institute, 20 Staniford Street, Boston, MA 02114. Investigative Ophthalmology & Visual Science, September 1998, Vol. 39, No. 10 Copyright © Association for Research in Vision and Ophthalmology Downloaded From: http://iovs.arvojournals.org/ on 06/17/2017 in 1991 that immune privilege is extended to allogeneic tumor cells injected into the vitreous cavity (VC).10 Because an important goal of our research is to transplant retinal tissues into the subretinal space, our interests were focused on immune reactions after inoculation of antigens into the subretinal space. Experiments reporting long-term survival of grafts and suppression of DTH after transplantation of neonatal ocular tissue into the subretinal space 1112 suggest that the subretinal space elicits immune deviation, resembling ACAID. However, the transplanted tissues in those experiments were eye derived and therefore probably represent immune-privileged tissues, which may considerably alter the observed immune responses. Anatomically, the subretinal space is a potential space located between the retinal pigment epithelial (RPE) layer and the photoreceptor outer segments. This space becomes visible in retinal detachment when the photoreceptors lose contact with the RPE cell layer. Because there is free fluid exchange between the photoreceptor layer and the subretinal space, the functional borders of the subretinal space are defined by the RPE layer and by the outer limiting membrane of the retina. The RPE lining the outer border of the subretinal space forms the outer blood-retinal barrier and allows exchange of molecules and cells only through the RPE layer itself.13 The inner barrier is formed by close intercellular contacts (zonula adherens) among Miiller cell processes with a pore size of approximately 300 nm to 360 nm, permitting the exchange of smaller 1823 1824 Wenkel and Streilein FA Downloaded From: http://iovs.arvojournals.org/ on 06/17/2017 10VS, September 1998, Vol. 39, No. 10 IOVS, September 1998, Vol. 39, No. 10 molecules for the nutrition of the outer part of the neurosensory retina. Normally, the space is filled with the so-called interphotoreceptor matrix, a complex mixture of soluble and nonsoluble substances, including hyaluronic acid, chondroitin sulfate, interphotoreceptor retinol-binding protein, and transforming growth factor-/3. However, the exact composition is still unknown.13 Importantly, this space is supposed to be devoid of bone marrow- derived cells. Although the main function of the interphotoreceptor matrix is the support and maintenance of photoreceptor segments, the space may also have immunologic functions, because the subretinal space has a strategic location between the retina and the choroid. Many immunologic and infectious diseases of the posterior part of the eye take place at this site. Our experimental goal was to determine whether a deviant immune response, similar to ACAID,2'6 could be induced after injection of cell-associated or soluble antigens into the subretinal space. In addition, we sought to identify parameters essential for the capacity of the subretinal space to induce antigen-specific immune deviation. Sodium iodate was used to disrupt the outer blood-retinal barrier by compromising RPE cells,14 and mild corneal cauterization was used to abolish ACAID and immune privilege in the anterior chamber.'5 MATERIALS AND METHODS Animals Adult female BALB/c mice aged 6 to 8 weeks were obtained from the animal facilities at the Schepens Eye Research Institute or from Jackson Laboratories (Bar Harbor, ME). Mice were maintained in a common room of a vivarium. Inoculations, injections, clinical examinations, and enucleations were conducted in animals under anesthesia induced by intraperitoneal injection of 0.075 mg/kg ketamine (Ketalar; Parke Davis, Paramus, NJ), and 0.006 mg/kg xylazine (Rompun; Phoenix Pharmaceutical, St. Joseph, MO). All experimental procedures conformed to the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research. Five mice comprised each group, and experiments were repeated at least twice. Intraocular Injections For AC, VC, and subconjunctival injections, a 0.3-mm penetrating wound was made with a 30-gauge needle in the peripheral portion of the cornea, 1 mm posterior to the limbus, or in the fornix of the conjunctiva, respectively. For injections in the subretinal space, the temporal conjunctiva was opened parallel to the limbus, and the eye was rotated to expose the posterior part of the sclera. A 0.3-mm tangential sclerotomy was made with a 30-gauge needle, and a retinal bleb was created with 0.5 Subretinal Space-Associated Immune Deviation 1825 /xl Hank's balanced salt solution. Tumor cells or ovalbumin were injected into the wound. Tumor Cells and Injections P815 mastocytoma cells (DBA/2 origin) were grown as previously described.4 For experiments, 2 X 105 P815 cells in a volume of 2 /xl were slowly injected into the ocular wound using a glass micropipette connected to a 10-/LXI syringe. Fifty micrograms ovalbumin in Hank's balanced salt solution was used as a soluble antigen and was injected in a volume of 2 /xl into the eyes of experimental animals. Sodium Iodate Injections and Horseradish Peroxidase Method Sodium iodate, a substance especially toxic to RPE cells' 4 was used to disrupt the outer blood-retinal barrier. Experimental animals received a single injection of 40 mg/kg sodium iodate (0.08 M at pH 7.4) in a tail vein 30 or 2 days before antigen injection. For evaluation of outer bloodretinal barrier breakdown through extravasation of horseradish peroxidase (HRP), control animals (no tumor cell injection) received an intravenous (IV) injection of 200 mg/kg HRP type II 15 minutes before they were killed at different time points (6, 12, and 24 hours and 2, 3, 7, 14, and 21 days). Eyes were enucleated and fixed for 30 minutes in ice-cold 10% buffered formalin. Tissue was then processed for frozen sections and 5-/xm sections were cut. Sections were reacted with 3,3-diaminobenzidine and hydrogen peroxidase and evaluated by means of light microscopy."' Cauterization of the Cornea To alter immune privilege in die AC, the left cornea of experimental animals was cauterized as previously described.15 Briefly, the midperiphery of the cornea was cauterized at five points by means of electrocautery, until a white discoloration was seen. Seven days after cauterization, antigens were inserted into the AC, VC, or subretinal space. Clinical Evaluation of Tumor Growth of P815 Cells The anterior segment of eyes after tumor cell inoculation was examined clinically with a slit lamp (Topcon; Tokyo Optical, Tokyo, Japan), and the posterior segment was examined with a dissecting microscope through a coverslip. All eyes were examined at 3, 7, and 14 days after injection of tumor cells. Histopathologic Evaluation of Tumor Growth of P815 Cells For histologic evaluation, tumor-bearing control eyes of each group were enucleated at different time points (3, 7, FIGURE 1. Photomicrographs of sections of the retina of mice enucleated 15 minutes after systemic injection of horseradish peroxidase to evaluate the integrity of the blood-retinal barrier. After sections were reacted with 3,3-diaminobenzidine and hydrogen peroxidase, they were evaluated for extravascular detection of horseradish peroxidase. (A) Retina of mouse eye enucleated 12 hours after systemic injection of sodium iodate. Extravascular dark granular staining of the subretinal space and the photoreceptor segments show leakage of horseradish peroxidase through the outer blood-retinal barrier. (B) Retina of mouse eye enucleated 48 hours after systemic injection of sodium iodate. Positive peroxidase staining is visible extravascularly throughout the entire retina. (C) Retina of mouse eye enucleated 21 days after systemic injection of sodium iodate. Positive staining of endogenous peroxidase is seen only in intravascular erythrocytes of retinal and choroidal vessels. No extravascular leakage of horseradish peroxidase into the retina was detected. Downloaded From: http://iovs.arvojournals.org/ on 06/17/2017 1826 Wenkel and Streilein IOVS, September 1998, Vol. 39, No. 10 and 14 days), fixed with 10% buffered formalin, processed for routine histopathology and stained with hematoxylin- eosin. Assay for DTH Delayed-type hypersensitivity for cell-associated antigens was evaluated 14 days after inoculation of P815 tumor cells. For soluble antigen, 7 days after intraocular inoculation of ovalbumin, animals were immunized subcutaneously with 100 /Ltg ovalbumin and complete Freund's adjuvant. Ear-swelling analysis was performed 7 days later. Delayed-type hypersensitivity was measured based on ear swelling, as previously described.12 Briefly, 5 X 105 P815 tumor cells (irradiated with 2000 R) or 200 jttg ovalbumin in 10 /u.1 Hanks' balanced salt solution were injected into the left ear pinnae of the mice. The right ear served as untreated control. Both ear pinnae were measured immediately before injection and 24 hours later with an engineer's micrometer (Mitutoyo, Tokyo, Japan). The measurements were performed in triplicate. Results were expressed as specific ear swelling = (24-hour measurement — 0-hour measurement in the experimental ear — 24 hour measurement — 0-hour measurement in the negative control ear) X 10~3 mm. A two-tailed Student's £-test was used, with significance assumed at P < 0.05. For graphic illustrations, the mean of negative control measurements was subtracted from single values in each experiment. Adoptive Transfer Assay for Suppression of DTH For adoptive transfer studies for cell-associated antigens, splenocytes obtained from ocular (P815 cell) tumor-bearing mice were injected IV (6 X 107 spleen cells) into naive BALB/c mice, as previously described.10 In negative and positive control animals, the same amount of spleen cells of naive BALB/c mice was infused IV. To control for antigen-specificity of adoptive transfer, a control group of mice received an injection of a nonrelevant antigen (ovalbumin) into the subretinal space; spleen cells from these mice were later transferred into naive mice. Within 2 hours all experimental mice, the specificity control animals, and the positive control animals received a subconjunctival injection of 2 X 105 P815 cells. Negative control mice received no subconjunctival tumor cell injection. Delayedtype hypersensitivity was assayed 10 days after transfer of spleen cells. For soluble antigens, naive mice received an IV injection of splenocytes (6 X 107 cells) derived from animals that had received 50 jag ovalbumin intraocularly 7 days before adoptive transfer. Twenty-four hours later, all experimental mice and the positive control mice were immunized with 100 /xg ovalbumin and CFA subcutaneously. Negative control mice were not immunized. Ten days later DTH was assayed. 60 . Scon AC SR Naive 2. Delayed-type hypersensitivity measurement after injection of P815 cells into different ocular sites. Ear-swelling analysis 24 hours after ear challenge with P815 cells in animals with inoculation of P815 cells into the anterior chamber (AC) or the subretinal space (SR) 14 days earlier. As a positive control, animals had received P815 cells subconjunctivally (Scon). Naive animals that underwent ear challenge only served as negative control. *Significantly lower mean response than in positive control animals (P < 0.05). FIGURE Downloaded From: http://iovs.arvojournals.org/ on 06/17/2017 Subretinal Space-Associated Immune Deviation IOVS, September 1998, Vol. 39, No. 10 1827 80 - Naive AC SR Control OVA FIGURE 3. Adoptive transfer of impaired delayed-type hypersensitivity response induced by P815 cells in allogeneic BALB/c mice. Naive animals had received spleen cells intravenously from mice bearing P815 tumor cells in the anterior chamber (AC) or the subretinal space (SR) for 12 days. Control groups received spleen cells from naive animals. Specificity, control group (OVA) mice received spleen cells harvested from animals after ovalbumin injection into the subretinal space. One hour later, all recipients of spleen cells received 2 X 105 P815 cells into the subconjunctival space. Negative control animals (Control) received no subconjunctival tumor cell injection. Ten days later, delayed-type hypersensitivity was evaluated by ear-swelling analysis. *Significantly lower mean response than positive control (Naive; P < 0.05). RESULTS Alteration of Outer Blood-Retinal Barrier After IV injection of sodium iodate, breakdown of the outer blood-retinal barrier was detected by extravascular HRP within 12 hours (Fig. 1A). During the first 24 hours after inoculation, leakage of HRP was confined to the subretinal space and the photoreceptor region. After 2 days, peroxidase staining was detected throughout the entire retina (Fig. IB). There was no leakage of HRP out of retinal vessels or through vessels of the ciliary body or iris. Slowly, the barrier re-formed, so that by day 14, intravenous injected HRP only weakly stained the retina. By day 21, no extravascular HRP was detected in the retina. (Fig. 1C). Sodium iodate caused no gross morphologic changes in the RPE, examined by light microscopy, and no macrophages or lymphocytes were observed in the subretinal space. In addition, no choroiditis was detected after sodium iodate injection. Immune Deviation to Cell-Associated Antigens in the Subretinal Space Mice bearing P815 cells in the AC, the subretinal or the subconjunctival space were evaluated for DTH by ear challenge Downloaded From: http://iovs.arvojournals.org/ on 06/17/2017 assay 14 days after tumor cell inoculation. Results shown in Figure 2 indicate that after inoculation of tumor cells into AC or subretinal space, respectively, no DTH was detectable. By contrast, recipients of subconjunctivally injected tumor cells displayed intense DTH. Moreover, spleen cells adoptively transferred from animals bearing intraocular tumors into naive recipients suppressed DTH when the latter mice received a subconjunctival immunization with P815 cells (Fig. 3). In contrast, control animals that had received spleen cells from naive donors or from donors that received a nonrelevant antigen (ovalbumin) intraocularly showed intense DTH after subconjunctival immunization with P815 cells. Growth Patterns of Tumor Cells Injected Intracamerally P815 cells that have been injected into AC and VC develop into a progressively growing intraocular neoplasm.410 We compared the growth potential of P815 cells injected into the subretinal space with cells similarly injected into the AC. We observed that tumor cells inoculated into both sites grew progressively in eyes of all mice through day 14, at which time the eyes were enucleated (data not shown). As visualized by indirect ophthalmoscopy, early tumor growth in the subretinal 1828 Wenkel and Streilein Positive IOVS, September 1998, Vol. 39, No. 10 Naive AC VC SR 4. Delayed-type hypersensitivity measurements after inoculation of ovalbumin (OVA) in different ocular sites. Ear-swelling analysis was performed 24 hours after ear challenge with ovalbumin in animals that had had subcutaneous immunization with ovalbumin and complete Freund's adjuvant 7 days earlier. One week before immunization, ovalbumin was injected into the anterior chamber (AC), the vitreous cavity (VC), or the subretinal space (SR). Negative control animals (Naive) were not immunized. Positive control mice were immunized only. *Significantly lower mean response than in positive control animals (P < 0.05). FIGURE space (days 3-7) proceeded mainly horizontally (underneath the retina). Later, the cells penetrated into the choroid and into the vitreous cavity. By contrast, tumor cells injected into the subconjunctival space were rejected promptly. After an initial growth that was detectable by day 7, the subconjunctival masses quickly disappeared. Immune Deviation to Soluble Antigens in the Subretinal Space After injection of ovalbumin in the AC, the VC, the subretinal space, or the subconjunctival space, animals were immunized with ovalbumin and CFA at day 7. Evaluation by ear challenge analysis was performed 10 days later. No DTH was detectable after intraocular injection (AC, VC, or subretinal space) of ovalbumin (Fig. 4). By contrast, mice immunized with ovalbumin-CFA without prior intraocular injection of ovalbumin displayed intense DTH. Moreover, spleen cells adoptively transferred from animals after intraocular injection of ovalbumin suppressed DTH when the latter mice received a subcutaneous immunization with ovalbumin and CFA (Fig. 5). In contrast, control animals that had received spleen cells from naive mice showed intense DTH after subcutaneous immunization. Downloaded From: http://iovs.arvojournals.org/ on 06/17/2017 Effect of Sodium Iodate on Immune Privilege and Deviation in the Subretinal Space As shown earlier, IV injection of sodium iodate transiently compromised the outer blood-retinal barrier in mice by affecting RPE cell function. In the next experiments, we evaluated whether antigens injected into the subretinal space of these mice could support induction of immune deviation. Ovalbumin or P815 cells were injected into the subretinal space of eyes of mice treated two days earlier with sodium iodate. Recipients of P815 cells underwent ear challenge 10 days later with irradiated P815 cells. Recipients of ovalbumin were immunized subsequently with ovalbumin plus CFA, and ear challenge was conducted as before. Neither ovalbumin nor P815 cells injected into eyes compromised by sodium iodate acquired immune deviation (Figs. 6, 7). Instead, these mice showed intense, antigen-specific DTH. Moreover, tumor cells injected into the subretinal space after sodium iodate treatment failed to establish progressively growing tumor masses, and were eventually rejected (data not shown). When ovalbumin was injected into eyes of mice treated with sodium iodate 30 days earlier, the mice mounted strong DTH responses (Fig. 7); that is, they displayed no immune deviation. Subretinal Space—Associated Immune Deviation IOVS, September 1998, Vol. 39, No. 10 Naive AC SR 1829 Control FIGURE 5- Adoptive transfer of impaired delayed-type hypersensitivity response to ovalbumin. Naive animals received spleen cells intravenously from mice that had undergone anterior chamber (AC) or subretinal (SR) injection of ovalbumin 7 days earlier. Control groups received spleen cells from naive animals. One day later, all recipients of spleen cells were immunized with ovalbumin and complete Freund's adjuvant subcutaneously. Negative control animals (Control) were not immunized. Ten days later, delayed-type hypersensitivity was evaluated by ear-swelling analysis. 'Significantly lower mean response than in positive control (Naive) animals (/> < 0.05). Effect of Sodium Iodate on Immune Privilege in the Anterior Chamber and the Vitreous Cavity The previous experiments indicate that immune privilege in the subretinal space was lost for at least 30 days after sodium iodate treatment of mice. Even though we found no histologic evidence of compromise of the other vascular barriers in the eye (retinal vessels, vessels of iris and ciliary body), we wondered whether loss of immune privilege in the subretinal space would interfere with immune privilege in the anterior segment or the VC. Accordingly, groups of mice that had been pretreated with sodium iodate received inoculations of P815 cells or ovalbumin into the AC or the VC (only tested for ovalbumin), respectively. Subsequently, ovalbumin recipients were immunized with ovalbumin in CFA. Ear challenge of mice after injection of ovalbumin into the VC resulted in vigorous DTH (Fig. 7). When ears of mice after AC injection were challenged with P815 cells or ovalbumin, they displayed impaired DTH (Figs. 6, 7, respectively). Moreover, P815 cells injected into the AC of eyes of sodium iodate-treated mice grew progressively, as they do in eyes of normal mice.16 These results indicate that transient loss of immune privilege in the subretinal space correlated with loss of immune privilege in the VC, but did not simultaneously disrupt immune privilege in the AC. Downloaded From: http://iovs.arvojournals.org/ on 06/17/2017 Effect of Loss of Immune Privilege in the Anterior Chamber on Immune Privilege and Deviation in the Subretinal Space and the Vitreous Cavity Immune privilege in the AC can be abrogated by a variety of experimental manipulations, including light cauterization of the central corneal surface. Injection of antigens into the AC of eyes with corneas cauterized 1 week previously does not induce ACAID1517 (Figs. 8, 9). To determine whether loss of privilege in the AC has a disruptive effect on immune privilege in the subretinal space or the VC, corneal surfaces of eyes of BALB/c mice received light cautery. One week later, ovalbumin or P815 cells were injected into the subretinal space or the VC (only performed for ovalbumin). Subsequently, the ovalbumin recipients were immunized with ovalbumin and CFA. When ears of these mice were challenged with ovalbumin and P815 cells, intense DTH was observed (Fig. 8, 9). Moreover, growth of tumor cells in the subretinal space of eyes with cauterized corneas was severely curtailed, compared with the unrestricted growth of tumors in the subretinal space of normal mice. DISCUSSION Our experiments show that the subretinal space is an immunologically privileged site for cell-associated and soluble anti- 1830 Wenkel and Streilein IOVS, September 1998, Vol. 39, No. 10 80 - 0- Scon SR SR/SI AC AC/SI Naive FIGURE 6. Delayed-type hypersensitivity measurement after intraocular injection of P815 cells with or without pretreatment with sodium iodate. Delayed-type hypersensitivity was assessed by ear-swelling analysis 24 hours after ear challenge with P815 cells. Before ear challenge, animals were injected with P815 cells into subretinal (SR) space or anterior chamber (AC). Groups of animals had received systemic administration of sodium iodate (AC/SI; SR/SI) 2 days earlier. As a positive control, animals received P815 cells subconjunctivally (Scon). Naive animals with ear challenge only served as a negative control. *Significantly lower mean response than in positive control animals (JP < 0.05). gens. After injection into the subretinal space, allogeneic tumor cells grow progressively, and DTH is suppressed, compared with the effect of tumor cells injected into the subconjunctival space. The characteristics discovered so far resemble the findings in the AC: prolonged survival of allogeneic cells, suppressed DTH expression (immune deviation), and acquisition of splenocytes that can adoptively suppress DTH in naive animals.' "7 Although we did not analyze further the regulatory cells responsible for immune deviation in the subretinal space, we assume that the relevant splenic cells bear Thy 1.2 and CD8, as reported for the AC.3 However, there are certainly immune differences between the AC and the subretinal space. For example, we know that aqueous humor drains directly into the blood, which explains why the spleen serves as the focus of ACAID-inducing signals from the AC. We do not know how the signals that generate immune deviation after antigen injection into the subretinal space or the VC escape from the eye. It is possible that the antigen-bearing signals responsible for immune privilege from the subretinal space escape through the choroid and therefore travel through lymph vessels to cervical lymph nodes. Egan et al.18 reported T-cell expansion in the draining lymph nodes after intravitreal injection of antigen. In addition, they found greater T-cell expansion within the periarteriolar lymphoid splenic sheaths of VC injected mice, compared with that in mice immunized Downloaded From: http://iovs.arvojournals.org/ on 06/17/2017 through a conventional site. Whether these findings indicate differences between immune deviation in the AC and in the posterior part of the eye must await further investigations. Another issue is the antigen-specific signal within the blood that confers immune deviation in naive mice. For the AC, this signal proved to be F4/80+ monocytes that seem to arise from the stroma of iris, ciliary body, and trabecular meshwork.l9 The potential equivalents in the posterior part of the eye may be resident dendritic cells in the choroid, or microglia present within the neuroretina. Our current experiments explore these possibilities. Our present findings support the results of other investigators who found prolonged survival of neuroretinal or RPE grafts in the subretinal space. 1112 We are mindful of the likelihood that neuronal retinal tissue and RPE (used in previous experiments) may themselves represent immune-privileged tissues, and conclusions about immune privilege in the subretinal space based on experiments using immune-privileged tissues as grafts must therefore be circumspect. Sertoli cells are a case in point. These cells normally reside in an immune-privileged site, the testis. However, Sertoli cells show prolonged survival when implanted in non-immune-privileged sites, such as beneath the kidney capsule.20 In the present experiments we used P815 cells, which are non-immune-privileged cells that IOVS, September 1998, Vol. 39, No. 10 Subretinal Space-Associated Immune Deviation 1831 60 (/) 0) C o 20 10 Positive AC/SI SR/SI(30d) Naive FIGURE 7. Delayed-type hypersensitivity measurement after intraocular injection of ovalbumin with or without pretreatment with sodium iodate. Ear-swelling analysis was performed 24 hours after ear challenge with ovalbumin in animals immunized subcutaneously with ovalbumin and complete Freund's adjuvant 7 days earlier. One week before immunization, ovalbumin was injected into the anterior chamber (AC), the vitreous cavity (VC), or the subretinal space (SR). Groups of animals had received systemic administration of sodium iodate (SR/SI; VC/SI; AC/SO 2 days earlier. One group of animals had received a single systemic injection of sodium iodate 30 days before subretinal inoculation of ovalbumin (SR/SI[30 d]). Negative control animals (Naive) were not immunized. Positive control mice were immunized only. 'Significantly lower mean response than in positive control animals (P < 0.05). are readily rejected at conventional sites.16 Nonetheless, P815 cells showed prolonged survival in the subretinal space. Based on our experimental results and the findings of others, we conclude that the subretinal space is an immuneprivileged site. However, the site seems unable to confer indefinite survival on foreign tissue grafts placed within it, whether the experiments are conducted in animals or in human eyes.">2' Especially because retinal transplantation will eventually be considered for diseased human eyes, the privileged nature of this site may be open to question. Therefore, it seemed important to determine the extent to which immune privilege and immune deviation are lost when the properties of the subretinal space are altered experimentally. As a case in point, in the human disease age-related macular degeneration, the rejection rates of allogeneic human RPE grafts are reported to be lower in nonexudative than in neovascular age-related macular degeneration.21 To evaluate the immune consequences of altering immune privilege in the subretinal space, two techniques were used in our study: The RPE layer was compromised with a prior IV injection of sodium iodate, which led to a breakdown of the outer blood-retinal barrier, whereas immune privilege in the AC was broken by corneal thermal injury. Downloaded From: http://iovs.arvojournals.org/ on 06/17/2017 To test the influence of a disrupted outer blood-retinal barrier, we injected sodium iodate IV before inoculation of antigen. Sodium iodate is known to be almost exclusively toxic to RPE cells, resulting in a rapid loss of barrier function.1'* In general, effects on the systemic immune system of experimental animals treated with sodium are thought to be trivial.22 For example, experimental allergic encephalomyelitis develops unimpeded in animals pretreated with sodium iodate.23 However, sodium iodate may have an effect on S-antigen-induced experimental autoimmune uveitis. High doses of sodium iodate decreased the rate of experimental autoimmune uveitis developing in a susceptible rat strain,23 whereas low-dose sodium iodate in a primarily unsusceptible rat strain promoted the development of experimental autoimmune uveitis.2'1 These dose-dependent effects may be caused by different degrees of RPE damage and blood-retinal barrier breakdown, which could alter the course of experimental autoimmune uveitis. Whether the impairment of the RPE cells by sodium iodate has a direct effect on immune mechanisms in the eye can only be speculated. In our animals the outer blood-retinal barrier was disrupted for approximately 14 days after sodium iodate injection, after which repair began, and the barrier was eventually re- 1832 Wenkel and Streilein IOVS, September 1998, Vol. 39, No. 10 70 • Scon SR SR/Cau AC AC/Cau Naive 8. Delayed-type hypersensitivity measurement after intraocular injection of P815 cells with or without previous corneal cauterization. Delayed-type hypersensitivity was assessed by ear-swelling analysis 24 hours after ear challenge with P815 cells. Before ear challenge, animals were injected with P815 cells into the subretinal (SR) space or anterior chamber (AC). Corresponding groups of animals had received corneal cauterization 7 days before anterior chamber (AC/Cau) or subretinal (SR/Cau) injection of P815 tumor cells. As a positive control, animals received P815 cells subconjunctivally (Scon). Naive animals that had ear challenge only served as a negative control. *Significantly higher mean response than corresponding group of animals without previous corneal cauterization (P < 0.05). FIGURE established. We found that injection of sodium iodate a few days before antigen inoculation into the subretinal space abolished immune privilege and abrogated immune deviation to cell-associated and soluble antigens in the subretinal space and the VC. Experiments performed with ovalbumin in mice pretreated with sodium iodate 30 days earlier showed persistently impaired immune deviation, even though the blood-retinal barrier is believed to re-form within 3 weeks. This suggests that loss of immune privilege after injection of sodium iodate is not simply caused by breakdown of the blood-retinal barrier. One possible explanation for sustained loss of immune privilege after sodium iodate may be the entry of plasma proteins into the subretinal space. Serum proteinases could lower the concentration of transforming growth factor-/3 (or other immunosuppressive factors) in the subretinal space, thwarting the induction of immune deviation. Transforming growth factor-j3 is a major factor known to be important in immune deviation in the AC,25 and it is also present in die vitreous body.26 Re-establishment of this subretinal microenvironment after breakdown of the outer blood-retinal barrier could require a prolonged period. Another possible explanation for sustained loss of immune privilege in the subretinal space after sodium iodate injection could be prolonged impairment of RPE cell function. Retinal pigment epithelial cells are probably involved in creating the Downloaded From: http://iovs.arvojournals.org/ on 06/17/2017 appropriate microenvironment in the subretinal space through secretion of relevant cytokines and growth factors. However, until now very little has been known about the factors that promote immune deviation in the posterior part of the eye or their cellular origin. Therefore, a functionally intact RPE layer with an undisturbed outer blood-retinal barrier seems to be critical in the maintenance of the local microenvironment required for induction of immune deviation in the posterior part of the eye. Breakdown of this barrier may consequently cause the failure of allografts placed in the subretinal space. It is interesting that short-term abrogation of the outer blood-retinal barrier had little or no effect on immune privilege and deviation in the AC. Although tumor cells did not establish successful tumors in subretinal spaces with disrupted outer blood-retinal barriers, they grew well in the AC. In addition, immune deviation to minor histoincompatibility antigens and to soluble ovalbumin was readily induced through the AC of eyes of sodium iodate-treated mice. It has been reported that ACAID cannot be induced by injecting exogenous antigens into die AC of eyes of transgenic mice with selective expression of interferon-7 in photoreceptor cells.27 This report clearly indicates that alterations in the cytokine milieu of the subretinal space can interfere with ACAID in the AC. In these transgenic mice, expression of interferon-y is Subretinal Space-Associated Immune Deviation IOVS, September 1998, Vol. 39, No. 10 1833 70 - Positive SR SR/Cau VC VC/Cau AC AC/Cau Naive 9- Delayed-type hypersensitivity measurement after intraocular injection of ovalbumin with or without previous corneal cauterization. Ear-swelling analysis was performed 24 hours after ear challenge with ovalbumin in animals immunized subcutaneously with ovalbumin and complete Freund's adjuvant 7 days earlier. One week before immunization, ovalbumin was injected into the anterior chamber (AC), the vitreous cavity (VC), or the subretinal space (SR). Corresponding groups of animals had received corneal cauterization 7 days before anterior chamber (AC/Cau), intravitreal (VC/Cau), or subretinal (AR/Cau) injection of ovalbumin. Negative control animals (Naive) were not immunized. Positive control mice were immunized only. 'Significantly higher mean response than in corresponding group of animals without previous corneal cauterization (P < 0.05). FIGURE constitutive and constant, once the rhodopsin gene is upregulated. Therefore, we suspect that retention of immune privilege in the AC in the face of loss of the outer blood-retinal barrier may relate to the transient nature of the disruption caused in our experiment. Perhaps, a sustained loss of this barrier would lead eventually to loss of privilege in the vitreous cavity and the AC, but we have not yet achieved a stable disruption of the outer blood-retinal barrier to test this possibility. The potential for communication among the subretinal space, the VC, and the AC is relevant to ocular immune privilege, because our experiments indicate that abrogation of immune privilege in the AC (by corneal cauterization) can lead to loss of privilege and immune deviation in the subretinal space and the VC. Our experiments gave no hint of why loss of privilege in the AC should spread to the subretinal space, but we consider two possible explanations worthy of inquiry. First, after corneal cauterization, cells of the iris and ciliary body in that eye lose their capacity to secrete immunosuppressive factors.15 It is possible that modulators constitutively released from these cells reach the posterior compartment, even the subretinal space, and contribute to the normal immunosuppressive microenvironment. Thus, a resultant deficit of immunosuppressive factors from iris and Downloaded From: http://iovs.arvojournals.org/ on 06/17/2017 ciliary body cells could rob the subretinal space of its immunomodulatory properties and thereby its immune privilege. Second, cautery of the cornea may alter indirectly the properties of the outflow tract through which antigenic information must pass to induce immune deviation. If the only route of escape for antigens from the subretinal space and the VC that induces immune deviation is anteriorly through the trabecular meshwork, and if this pathway is altered after corneal cauterization, antigenic signals from the posterior part of the eye probably will not promote immune deviation. Experiments to test these possibilities are currently under way. References 1. Streilein JW. Immune regulation and the eye: a dangerous compromise. FASEBJ. 1987; 1:199-208. 2. Streilein JW, Niederkorn JY, ShadduckJA. Systemic immune unresponsiveness induced in adult mice by anterior chamber presentation of minor histocompatibility antigens. / Exp Meet. 1980; 152: 1121-1125. 3. Streilein JW, Niederkorn JY. Characterization of the suppressor cell(s) responsible for anterior chamber-associated immune deviation (ACA1D) induced in BALB/c mice by P815 cells. JT Immunol. 1985;134:1381-1387. 1834 Wenkel and Streilein 4. Ksander B, Streilein JW. 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