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6646 Nucleic Acids Research, Vol. 19, No. 23 Minos, a new transposable element from Drosophila hydei, is a member of the Tc1-like family of transposons Gerald Franz1 + and Charalambos Savakis 12 * institute of Molecular Biology and Biotechnology, Research Center of Crete, FO.R.T.H, PO Box 1527, Heraklion 711 10, Crete and department of Medical Sciences, University of Crete Medical School, Heraklion, Crete, Greece EMBL accession no. X61695 Submitted September 23, 1991 We have cloned and sequenced a new repetitive element from Drosophila hydei. The element was isolated in a screen of a genomic library from D. hydei strain bbx (1), performed to recover clones containing non-ribosomal DNA adjacent to ribosomal DNA sequences. A sequence 1775 nucleotides long was found inserted within the external transcribed spacer the rDNA locus, between bases 4257 and 4258 of the published sequence (2) (Figure 1). Southern blots of restricted DNA from two D. hydei strains showed distinct banding patterns (Figure 1), suggesting that the element is, or has until recently been mobile. The element was named Minos, after the legendary king who inhabited the palace located near our laboratories. The sequence of Minos has the following salient features: 1. Perfect inverted repeats 255 nt long (nucleotides 1 to 255 and 1521 to 1775) are found at the ends of the element. 2. Two non-overlapping open reading frames exist on the same strand. The first, designated ORF1, is located between nucleotides 334 and 792, begins with an ATG, and can encode a 153 amino acid long peptide. The second, designated ORF2, is located between nucleotides 898 and 1476 and does not begin with an ATG. The peptide sequence encoded by ORF2 has significant similarity with the putative transposase encoded by the long open reading frame of the transposable element Tel of Caenorhabditis elegans (3). Of the 201 amino acid residues in the two aligned sequences, 64 (~ 32%) are identical and 95 (—47%) are related (Figure 2). Similar sequence homology was detected between ORF2 and regions of the other members of the Tel-like transposon family (4, 5, 6, 7). Based on the genomic distribution of the element, the presence of perfect terminal repeats, and the sequence similarity with Tel, we conclude that Minos is a newly discovered member of the widely dispersed class of Tel-like transposons. The sequenced element, because of the stop codon within the putative transposase gene, presumably cannot encode active transposase. REFERENCES 1. Franz.G., Kunz.W. and Grimm.Ch. (1983) Molec. Gen. Genet. 191, 74-80. 2. Tautz.D., Tautz.C, Webb,D. and Dover.G.A. (1987) J. Mol. Biol. 195, 525-542. 3. Rosenzweig,B., Liao,L.W. and Hirsh.D. (1983) Nucleic Acids Res. 11, 4201-4209. 4. Harris.L.J., Baillie.D.L and Rose.A.M. (1988) Nucleic Acids Res. 16. 5991-5997. 5. Harris.L.J., Prasad,S. and Rose.A.M. (1990)7. Mol. Evol. 30, 359-369. 6. Brierley.H L. and Potter.S.S. (1985) Nucleic Acids Res. 13, 485-500. 7. Brezinksy.L., Wang.G.V.L, Humphreys.T. and Hunt.J. (1990) Nucleic Acids Res. 18, 2053-2059. _t OflFJ . : ORF1 ft Figure 1. Minos sequences in the D. hydei genome. Top: Position of the cloned element within the rDNA repeat unit. Vertical arrows indicate the positions of £coRI (E) and Hindlll (H) sites; the horizontal arrow indicates the rDNA transcriptional start. The hatched box represents the HindUl-EcoRl fragment used for hybridization. Bottom: DNA from bb1 strain (lane A) and a wild-type strain (lane B) was digested with EcoRI, subjected to electrophoresis, blotted and hybridized with the Hindlll-EcoRl Minos DNA probe. The origin of electrophoresis is on the left. 3 LRCftQEIA ACKNOWLEDGEMENTS This work was supported by European Community Mediterranean Integrated Pregrain funds to the IMBB and by a European Community training fellowship to G.F. Figure 2. Alignment of Minos and Tel hypothetical peptides. Solid boxes between the sequences indicate identities and stippled boxes indicate conservative replacements. The Minos sequence begins with the first in-frame methionine upstream of the termination codon. * To whom correspondence should be addressed + Present address: Entomology Unit, IAEA Laboratories, A-244 Seibersdorf, Austria