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MAY 2011 TIGAR-TAT (Catalog #150-14T) Synonym: TP53-induced glycolysis and apoptosis regulator Description: TIGAR is a p53-inducible enzyme that catalyzes the hydrolysis of fructose-2-6-bisphosphate (F-2-6-BP) to fructose-6-phosphate and inorganic phosphate. F-2-6-BP is a powerful activator of 6-phosphofructose-1 kinase, the rate limiting enzyme of glycolysis. By lowering the intracellular level of F-2-6-BP, TIGAR expression leads to increased glucose processing via the pentose phosphate pathway, the major cellular source for NADPH. NADPH plays a key role in maintaining the cellular redox state by regenerating reduced glutathione, which is critical for cellular protection against mitochondrial-derived reactive oxygen species (ROS). Consequently, TIGAR expression modulates p53-induced apoptosis in response to ROS-associated DNA damage. Since elevated levels of F-2-6-BP are required for cell growth and Continued on next page Updated - PeproTech Posters Now available (see page 3 for ordering information) Pretreatment with recombinant human TIGAR-TAT for 4 hrs, using a concentration range 0.5 - 1.0 mg/ml, has been shown to protect U2OS cells from apoptosis induced by hydrogen peroxide. This activity demonstrates that recombinant human TIGAR-TAT can successfully enter the cell when added to exogenous media and, once in the cell, exerts the expected TIGAR biological activity. PeproTech’s QR code, scan with your Smart Phone. PeproTech USB Cytokine Index Chemokine Poster TNF Superfamily Poster Now available. This new USB drive Cytokine INDEX contains 312 pages of encyclopedic coverage of more than 700 cytokines. Get Social with PeproTech! See our latest products and promotions FGF Family Poster NEW - VEGF/PDGF Family Poster Join our social network! MANUFACTURER OF QUALITY CYTOKINE PRODUCTS • W W W . P E P R O T E C H . C O M TIGAR-TAT and vascular endothelium. It has been widely used as a molecular marker for the identification, isolation, and manipulation of hemopoietic stem cells and progenitors. CD34 can function as a regulator of hemopoietic cell adhesion by mediating the attachment of stem cells to bone marrow stromal cells or other bone marrow components The full length human CD34 is a 385 amino acid protein, consisting of a 31 amino acid signal sequence, a 74 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain and a 259 amino acid extracellular domain. Recombinant human sCD34 is a 259 amino acid polypeptide containing only the extracellular domain of the full length CD34 protein. Continued from page 1 proliferation, p53-induced TIGAR expression prevents outgrowth of cells harboring damaged DNA. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other intracellular proteins into primary as well as transformed cells. Recombinant human TIGAR-TAT expressed in E.coli is a 36 kDa protein containing 284 amino-acid residues, including the 271 residues of full-length TIGAR fused to a 13-residue C-terminal peptide containing the TAT transduction domain (GGYGRKKRRQRRR). GDF-7 (Catalog #120-37) Synonyms: Growth/Differentiation Factor-7, BMP-12 Description: GDF-7 belongs to the TGF-β superfamily of growth MANF (Catalog #450-06) and differentiation factors. It is expressed selectively by roof plate cells that are located in the developing embryonic central nervous system, and has been shown to influence the neuronal identity of cells within the central nervous system. GDF-7 has also been implicated in the formation, maintenance, and repair of certain cartilage and ligament tissue. Recombinant human GDF-7 is a 28 kDa disulfide linked homodimer containing two 129 amino acid residues Synonyms: ARMET, Arginine-rich protein (ARP) Description: MANF (Mesencephalic Astrocyte-derived Neurotrophic Factor) is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. It has been shown to promote survival, growth and function of dopamine specific neurons. MANF and its structural homolog CDNF, each contain an N-terminal saposin-like lipid binding domain, and a carboxyl-terminal domain, which is not homologous to previously characterized protein structures. MANF and CDNF can prevent 6-OHDA induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parkinson disease. Recombinant human MANF is an 18.1 kDa protein consisting of 158 amino acids including 8 cysteine residues. Fetuin A/AHSG (Catalog #140-13) Synonyms: Alpha-2-HS-glycoprotein, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotien Description: Fetuin A/AHSG is a human plasma glycoprotein belonging to the Cystatin family of proteases inhibitors. It is highly expressed in embryonic cells and adult hepatocytes, and is expressed to a lesser extent in monocytes/macrophages. Fetuin A/AHSG is a major serum protein component that exerts various calcium dependent physiological activities, and can mediate growth signaling in certain tumor cells. It also can act as a natural antagonist against specific TGF-β and BMP signaling proteins, Recombinant human HEK293 cell derived Fetuin-A/AHSG is a heterodimeric protein containing a 280 amino acid A chain and a 25 amino acid B chain. Due to glycosylation, recombinant Fetuin-A/AHSG migrates at an apparent molecular weight of 45 – 55 kDa by SDS-PAGE run under non-reducing conditions. sCD30 Ligand (Catalog #450-42) Synonyms: TNFSF8, CD153 , CD30L Description: CD30 ligand (CD30L) is a type-II membrane- associated glycoprotein protein belonging to the TNF superfamily and is expressed primarily on certain B cells, T cells, and monocytes. CD30L binds specifically to CD30 (receptor), which is expressed on activated, but not resting, B and T cells, in lymphomas and various chronically inflamed tissues. CD30L/ CD30 interactions initiate a signaling cascade that can ultimately lead to the activation of NF-KappaB. CD30L/CD30 signaling exerts pleiotropic effects on normal cells, including cell death, differentiation, or cell division. Certain diseases, including Hodgkins’s lymphoma, allergic inflammation, diabetes (in NOD mice), and mycobacterial infection can also be affected by CD30L/ CD30 signaling. The CD30L gene encodes for a 234 amino acid type II transmembrane protein, which contains a 37 amino acid cytoplasmic sequence, a 25 amino acid transmembrane domain and a 172 amino acid extracellular domain. Recombinant human soluble CD30L (sCD30L) is a 188 amino acid polypeptide corresponding to the extracellular domain, and contains an 8 residue N-terminal His -Tag. VAP-1 (Catalog #150-16) Synonyms: Vascular Adhesion Protein-1, SSAO, HPAO, Copper amine oxidase, Membrane primary amine oxidase Description: VAP-1 is a type II membrane cell adhesion protein sCD34 (Catalog #310-31) Synonyms: Hematopoietic progenitor cell antigen CD34 Description: CD34 is a highly glycosylated type I membrane protein that is selectively expressed on hematopoietic stem cells -2- belonging to the copper/topaquinone oxidase family. It is primarily expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. VAP-1 can catalyze the oxidative deamination of low molecular weight amines, and plays an important role in the migration of lymphocytes to inflamed tissue. Inhibition of VAP-1 can protect against inflammation related damage to certain injured tissues. Additionally, VAP-1 can function as a significant prognostic marker for certain cancers and cardiovascular diseases. Recombinant human VAP-1 is a mixture of monomeric and disulfide linked homodimeric forms of a 737 amino acid polypeptide corresponding to amino acids 27 to 763 of the VAP-1 precursor. PeproTech’s New Stem Cell Kits ESC/iPSC Characterization Kit Embryonic Stem or induced Pluripotent Stem (ES/iPS) cell characterization kits provide a convenient tool for examining the pluripotency of human or mouse ES/iPS cells using ES/iPS cell specific antibody markers. Immunofluorescence Analyses: Highlights Convenient: The ready-to-use kit contains all the buffers and solutions with optimized concentrations in addition to ES/iPS cell specific antibodies. Cost effective: The most competitive prices on the market Reliable: Each kit includes a panel of validated biomarkers: • For Human - anti-Oct4, Sox2, SSEA3, TRA-1-60, TRA-1-81, and Alkaline Phosphatase (AP) • For Mouse - anti-Oct4, Sox2, SSEA1, and AP Flexible: Various wavelength 2nd antibodies available upon request Product Oct4, mESC (A) Catalog # Human ESC/iPSC characterization kit (Green stain)........................................................ASK-3006G Human ESC/iPSC characterization kit (Red stain)............................................................ASK-3006R (20 reactions) Mouse ESC/iPSC characterization kit (Green stain).........................................................ASK-3005G Mouse ESC/iPSC characterization kit (Red stain).............................................................ASK-3005R (20 reactions) Antibodies - More New Products from PeproTech! Polyclonal/Biotinylated Development Kits New Animal-Free Proteins ELISA Human CTGF...................................... 900-K317 Human MIP-3a................................ AF-300-29A Human TGF-a................................. AF-100-16A Human IL-7.........................................AF-200-07 Human G-CSF....................................AF-300-23 Human NT-3.......................................AF-450-03 Human NT-4.......................................AF-450-04 Human RANTES................................AF-300-06 ELISA Buffer Kit .....................900-K00 Monoclonal Antibodies Anti-human IP-10................................. 500-M60 Anti-rat IL-10...................................... 500-M130 TRA-1-60, hESC (B) Murine IL-3............................................ 900-K48 Murine IL-21........................................ 900-K368 Murine RANTES................................. 900-K124 Rat RANTES......................................... 900-K72 Mini - ELISA Development Kits Human CTGF......................................900-M317 Murine IL-3............................................900-M48 Murine IL-21........................................900-M368 Murine RANTES.................................900-M124 Rat RANTES.........................................900-M72 -3- Anti-human sDLL-4.............................. 500-P279 Biotinylated Anti-hsDLL-4................500-P279bt Anti-human EGF receptor..................... 500-P306 Biotinylated Anti-hEGF receptor.......500-P306bt Anti-human IL-5................................... 500-P307 Biotinylated Anti-hIL-5.....................500-P307bt Anti-murine IL-5..................................... 500-P55 Biotinylated Anti-mIL-5......................500-P55bt Anti-human MEC.................................. 500-P297 Biotinylated Anti-hMEC....................500-P297bt Anti-human p16-INK4a-TAT..............500-P248T Biotinylated Anti-hp16-INK4a-TAT....500-P248Tbt Anti-human VCAM-1........................... 500-P300 Biotinylated Anti-hVCAM-1.............500-P300bt Anti-human Visfatin.............................. 500-P222 Biotinylated Anti-hVisfatin................500-P222bt Published by PeproTech 12 Hamada Street • Tamar Building Rehovot, 76703 • Israel Tel: +972 8 946 0948 • Fax: +972 8 946 0861 email: [email protected] www.peprotech.com